Olivia Mozley

Tel: 0114 222 7594

Biography

Prior to studying in Sheffield, I completed a BSc (hons) Biotechnology at the University of Edinburgh and an EPSRC funded PGDip Energy and Environment at the University of Leeds. My PhD is funded by the Sheffield A*STAR Research Attachment Programme.

Supervisor:
Professor David James

Research Group:
Biological and Environmental Systems Group

Research

A molecular dissection of polyethylenimine mediated transfection in CHO cells. My research is applicable to biopharmaceutical production through transient gene expression, for which large scale transfection of mammalian cells is required.

Publications

Thompson, B. , Segarra, C., Mozley, O., Daramola, O., Field, R., Levison, P. and James, D. (2012) Cell line Specific control of PEI-mediated transfection optimized with Design of Experiments methodology. Biotechnology Progress, 28, 179-187.

Presentations

Cell Line Development and Engineering, Vienna, February 2013. “A Mechanistc Dissection of Polyethylenimine Mediated Transfection of CHO cells. “ [Oral Presentation]
Cell Culture Engineering XIII, Arizona, USA - April 2012. “Control of polyplex mediated transfection of CHO cells”. [Poster]
ESACT-UK - January 2012 (poster prize winner) – “Recombinant protein production by transient gene expression: the mechanism of polyethylenimine mediated transfection of CHO-S cells”. [Poster]

Prizes

ESACT-UK poster prize 2012