Dr. Rahul Vijay Kapoore

Post-Doctoral Research Associate

Email: R.kapoore@sheffield.ac.uk or rahulkapoore@gmail.com

Advance Biomanufacturing Centre

Department of Chemical and Biological Engineering
University of Sheffield
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD

Tel: +44(0)114 222 7594

Fax: +44 (0)114 222 7501

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Research

Biography

I am a member of the SuBB research project, led by Dr Raman Vaidyanathan. My current research interest areas are;

  1. Developing an optimised workflow for microalgal metabolomics. Microalgae not only play an important ecological role, but are also of commercial importance and therefore call for an in depth knowledge of basic biological functions through metabolomics. With the current technologies only a portion of the metabolome can be analysed. As a result there is a drive to direct significant analytical efforts towards capturing metabolome or changes in the metabolome reliably and reproducibly. We are investigating and developing the optimized method for metabolomics using MS based hyphenated techniques. Apart from analytical challenges, the other challenges we are addressing include development of appropriate methodologies to quench the metabolism, techniques for reliable and comprehensive extraction of metabolites, data interpretation using statistical approaches, metabolite identification, preliminary construction of mass spectral libraries and well-curated metabolite databases and finally quality control and validation of all the steps involved in metabolomics pipeline.
  2. Photoautotrophic carbon uptake by microalgae for biotransformation’s
  3. Further fine tuning of biological species by genetic or metabolite engineering in combination with other systems biology approaches.
  4. In oncology, capturing an accurate metabolite signatures presents a challenge particularly for the evaluation of adherent cultures. The biology and pathways involved in TNBC is still very poorly understood, therefore there is demand for a comprehensive approach that will aid in defining the relative contribution of major metabolic pathways in TNBC. Given this, our current research mainly focuses on evaluating the coverage of major breast cancer pathways in the analysed metabolome for the TNBC cell lines with our optimised methods.

Research interests

• Bioanalytical technologies
• Systems biology approaches (Metabolomics, proteomics and transcriptomics)
• Lipidomics
• Bio-energy
• Oncology

Originally from Nashik (India), I obtained first class degree in Bachelor of Pharmacy in 2006 from M.G.V’s College of Pharmacy, Nashik (Pune University). Subsequent to the completion of my degree, I came to U.K. in 2006 to peruse my MSc in Pharmacology and Biotechnology at Sheffield Hallam University, which I graduated from in November, 2007.

I completed my PhD in metabolomics at The University of Sheffield in 2014 under the supervision of Dr Raman Vaidyanathan. My doctoral research involved development and optimisation of mass spectrometry based hyphenated techniques for microalgal and mammalian metabolomics. Simultaneously, I was also working as a Sheffield Graduate Trainee (Sept/10-July/14) at The University of Sheffield, where I was involved in numerous demonstration duties in chemical and biological engineering discipline. Briefly, I have conducted demonstration duties for masters and undergraduate students covering topics such as bioreactors, spectrophotometry, protein assays, biology tutorials, computing tutorials and engineering design weeks. I have also successfully conducted workshops for masters and PhD students on chromatographic and mass spectrometric techniques.

I joined the Department of Chemical and Biological Engineering at The University of Sheffield in August 2014 as a Postdoctoral Research Associate (PDRA) on a BBSRC funded project: “Sustainable Bioenergy from Microalgae: A Systems Perspective”. My current research involves metabolome level characterisations of microalgae cultures and thereby developing a systems level understanding in combination with other systems biology approaches (proteomics and transcriptomics), that will lead to sustainable processes for bio-energy generation from microalgae. Current research is ongoing in collaboration with other researchers in the Department and those in the Department of Molecular Biology and Biotechnology at Sheffield. In addition, the work also involves collaboration with researchers at the University of Cambridge (UK), The Scottish Association for Marine Science (Scotland) and two Indian Institutes (NFMC, Bharatidasan University, Tiruchirapalli (Funded by Department of Biotechnology (DBT), Govt. of India) and Lady Doak College, Madurai).

Principal Investigator

 Dr Raman Vaidyanathan

 

Publications

Key publications

Hounslow E, Kapoore RV, Vaidyanathan S, Gilmour DJ, Wright P (2016) “The Search for a Lipid Trigger: The Effect of Salt Stress on the Lipid Profile of the Model Microalgal Species Chlamydomonas reinhardtii for Biofuels Production” (CBIOT; DOI: 10.2174/2211550105666160322234434)

Chen Y, Tang X, Kapoore RV, Xu C, and Vaidyanathan S. (2015)
Influence of nutrient status on the accumulation of biomass and lipid in Nannochloropsis salina and Dunaliella salina.
(Energy Conversion and Management; DOI: 10.1016/j.enconman.2015.09.025)

Kapoore RV, Coyle R, Staton CA, Brown NJ, and Vaidyanathan S. (2015)
Cell line dependence of metabolite leakage in metabolome analyses of adherent normal and cancer cell lines.
Metabolomics, 11, 1743-1755.

Oral and Poster presentations

[P – Poster presentation; O/P – Both oral and poster presentation; C – Conferences attended]
[P1] Deeptanshu Pandey1, Rahul Vijay Kapoore1, Katarzyna Okurowska2, Seetharaman Vaidyanathan*
“Photoautotrophic carbon uptake by microalgae for biotransformation” Carbon Dioxide Utilisation: Faraday Discussion, The University of Sheffield, Sheffield, U.K., 7 – 9 September, 2015

[P2] Babakar Mevan1, Kapoore Rahul2, Pugh John2, Salim Malinda2, Vaidyanathan Seetharaman*
“Biochemical microscopy: ToF SIMS” KTN Industry Day, The University of Sheffield, Sheffield, U.K., Feb. 2014

[O1/P3] Kapoore Rahul1, Coyle Rachael2, Brown Nicola*, Vaidyanathan Seetharaman*
“Development & Optimization of GC-MS based global metabolite profiling of adherent mammalian cells” Metabolomics 2013, 9th Annual International Conference of the Metabolomics Society, Glasgow, Scotland, U.K., July 2013

[C1] Algae Biotechnology Sheffield Network First Annual Conference.
“Research in Algae Biotechnology at the University of Sheffield”
Idea Space, Chemical and Biological Engineering, Sheffield, U.K., July 2013

[O2/P4] Kapoore Rahul1, Chen Yimin2, Longworth Joseph3, Vaidyanathan Seetharaman*
“GC-MS based metabolite profiling in microalgae” Metabolomics 2012, Wardman Marriott Park Hotel, Washington D.C., U.S.A., June 2012

[P5] Pugh John1, Salim Malinda2, Kapoore Rahul3, Vaidyanathan Seetharaman*
“In situ Metabolomics via Vapour-mediated Ion Activation Enhanced SIMS” Metabolomics 2012, Wardman Marriott Park Hotel, Washington D.C., U.S.A., June 2012

[P6] Longworth Joseph1, Kapoore Rahul2, Wright Phillip*, Vaidyanathan Seetharaman*
“Carbon source effects on Chlamydomonas reinhardtii: A proteomic & metabolic investigation” ChELSI Conference, Sheffield, U.K., 2012

[C2] “RECENT ADVANCES AND FUTURE DIRECTIONS IN APPLIED METABOLOMICS” Agilent Applied Metabolomics. University of Manchester, Centre of Excellence in Biopharmaceuticals, Manchester, U.K., Feb. 2012