Dr Patrick Baker

School of Biosciences

Senior Lecturer

p.baker@sheffield.ac.uk
+44 114 222 2725

Firth Court

Full contact details

Dr Patrick Baker
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN

Profile

Career history

2010 - Senior Lecturer, School of Biosciences, The University of Sheffield
2015 - 2019 Director Of Studies and Deputy Head of Department, Dept of Molecular Biology and Biotechnology, The University of Sheffield
2010: Visiting Lecturer, Dept Bioquimica, Faculdad Sciencias, The University of Alicante, Spain
2008 -2010: Lecturer, Dept. of Molecular Biology and Biotechnology, The University of Sheffield
1993 -2010: Senior Experimental Officer (X-ray facility manager) in the Krebs Institute, University of Sheffield
1987-1993: Postdoctoral Research Associate, Dept. of Biochemistry, The University of Sheffield
1984-1987: PhD. Student, Dept. of Biochemistry, The University of Sheffield
1982-1984: Research Assistant, Dept. of Clinical Haematology, University College, London

Research interests

In my group we use X-ray crystallography to determine the atomic structure of biological macromolecules and thus to elucidate their structure/function relationships. We work closely with the other structural biology groups in Sheffield and have strong collaborations with numerous research groups across the world. Our research can be divided into three broad areas:

developing new antimicrobial targets
substrate specificity and chiral synthesis in enzymes
the molecular basis of extreme stability in proteins from extremophiles

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Cruz-Migoni A, Hautbergue GM, Artymiuk PJ, Baker PJ, Bokori-Brown M, Chang C-T, Dickman MJ, Essex-Lopresti A, Harding SV, Mahadi NM , Marshall LE et al (2011) A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.. Science, 334(6057), 821-824. View this article in WRRO
Tomlinson JH, Green VL, Baker PJ & Williamson MP (2010) Structural origins of pH-dependent chemical shifts in the B1 domain of protein G.. Proteins, 78(14), 3000-3016.
Baker PJ, Britton KL, Fisher M, Esclapez J, Pire C, Bonete MJ, Ferrer J & Rice DW (2009) Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily.. Proc Natl Acad Sci U S A, 106(3), 779-784.
Haydon DJ, Stokes NR, Ure R, Galbraith G, Bennett JM, Brown DR, Baker PJ, Barynin VV, Rice DW, Sedelnikova SE , Heal JR et al (2008) An inhibitor of FtsZ with potent and selective anti-staphylococcal activity.. Science, 321(5896), 1673-1675.
Xu L, Sedelnikova SE, Baker PJ & Rice DW (2008) Cloning, purification and preliminary crystallographic analysis of a putative DNA-binding membrane protein, YmfM, from Staphylococcus aureus.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 64(Pt 7), 656-658.
Xu L, Sedelnikova SE, Baker PJ & Rice DW (2006) Cloning, purification and preliminary crystallographic analysis of a conserved hypothetical protein, SA0961 (YlaN), from Staphylococcus aureus.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 62(Pt 8), 778-780. View this article in WRRO

All publications

Journal articles

Mobbs GW, Aziz AA, Dix SR, Blackburn GM, Sedelnikova SE, Minshull TC, Dickman MJ, Baker PJ, Nathan S, Raih MF & Rice DW (2022) Molecular basis of specificity and deamidation of eIF4A by Burkholderia Lethal Factor 1. Communications Biology, 5(1).
Ramly NZ, Dix SR, Ruzheinikov SN, Sedelnikova SE, Baker PJ, Chow Y-P, Tomley FM, Blake DP, Wan K-L, Nathan S & Rice DW (2021) The structure of a major surface antigen SAG19 from Eimeria tenella unifies the Eimeria SAG family.. Commun Biol, 4(1), 376.
Churchill-Angus AM, Schofield THB, Marlow TR, Sedelnikova SE, Wilson JS, Rafferty JB & Baker PJ (2021) Characterisation of a tripartite α-pore forming toxin from Serratia marcescens.. Sci Rep, 11(1), 6447.
Churchill-Angus AM, Sedelnikova SE, Schofield THB & Baker PJ (2020) The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis. Acta Crystallographica Section F Structural Biology Communications, 76(12), 577-582.
Wilson JS, Churchill-Angus AM, Davies SP, Sedelnikova SE, Tzokov SB, Rafferty JB, Bullough PA, Bisson C & Baker PJ (2019) Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila. Nature Communications, 10(1). View this article in WRRO
Ji S, Dix SR, Aziz AA, Sedelnikova SE, Baker PJ, Rafferty JB, Bullough PA, Tzokov SB, Agirre J, Li F-L & Rice DW (2019) The molecular basis of endolytic activity of a multidomain alginate lyase from Defluviitalea phaphyphila, a representative of a new lyase family, PL39. Journal of Biological Chemistry, 294(48), 18077-18091.
Subramanian K, Mitusińska K, Raedts J, Almourfi F, Joosten H-J, Hendriks S, Sedelnikova SE, Kengen SWM, Hagen WR, Góra A , Martins dos Santos VAP et al (2019) Distant non-obvious mutations influence the activity of a hyperthermophilic Pyrococcus furiosus phosphoglucose isomerase. Biomolecules, 9(6). View this article in WRRO
Dix SR, Owen HJ, Sun R, Ahmad A, Shastri S, Spiewak HL, Mosby DJ, Harris MJ, Batters SL, Brooker TA , Tzokov SB et al (2018) Structural insights into the function of type VI secretion system TssA subunits.. Nature Communications, 9. View this article in WRRO
Dix SR, Sun R, Harris MJ, Batters SL, Sedelnikova SE, Baker PJ, Thomas MS & Rice DW (2018) TssA from Aeromonas hydrophila: Expression, purification and crystallographic studies. Acta Crystallographica Section F: Structural Biology Communications, 74(9), 578-582. View this article in WRRO
Owen HJ, Sun R, Ahmad A, Sedelnikova SE, Baker PJ, Thomas MS & Rice DW (2018) TssA from Burkholderia cenocepacia: expression, purification, crystallization and crystallographic analysis. Acta Crystallographica Section F Structural Biology Communications, 74(9), 536-542. View this article in WRRO
Rawson S, Bisson C, Hurdiss DL, Fazal A, McPhillie MJ, Sedelnikova SE, Baker PJ, Rice DW & Muench SP (2018) Elucidating the structural basis for differing enzyme inhibitor potency by cryo-EM. Proceedings of the National Academy of Sciences of the United States of America, 115(8), 1795-1800. View this article in WRRO
Bisson C, Adams NBP, Stevenson B, Brindley AA, Polyviou D, Bibby TS, Baker PJ, Hunter CN & Hitchcock A (2017) The molecular basis of phosphite and hypophosphite recognition by ABC-transporters. Nature Communications, 8(1). View this article in WRRO
Saryi NA, Hutchinson JD, Al-Hejjaj MY, Sedelnikova S, Baker P & Hettema EH (2017) Pnc1 piggy-back import into peroxisomes relies on Gpd1 homodimerisation.. Scientific Reports, 7. View this article in WRRO
Hollingshead S, Bliss S, Baker PJ & Hunter CN (2016) Conserved Residues in Ycf54 are required for Protochlorophyllide Formation in Synechocystis sp. PCC 6803. Biochemical Journal. View this article in WRRO
Denby KJ, Iwig J, Bisson C, Westwood J, Rolfe MD, Sedelnikova SE, Higgins K, Maroney MJ, Baker PJ, Chivers PT & Green J (2016) The mechanism of a formaldehyde-sensing transcriptional regulator.. Scientific Reports, 6. View this article in WRRO
Bisson C, Britton KL, Sedelnikova SE, Rodgers HF, Eadsforth TC, Viner RC, Hawkes TR, Baker PJ & Rice DW (2016) Mirror-Image Packing Provides a Molecular Basis for the Nanomolar Equipotency of Enantiomers of an Experimental Herbicide. Angewandte Chemie International Edition, 55(43), 13485-13489. View this article in WRRO
Bisson C, Britton KL, Sedelnikova SE, Rodgers HF, Eadsforth TC, Viner RC, Hawkes TR, Baker PJ & Rice DW (2016) Mirror-Image Packing Provides a Molecular Basis for the Nanomolar Equipotency of Enantiomers of an Experimental Herbicide. Angewandte Chemie, 128(43), 13683-13687. View this article in WRRO
Bisson C, Britton KL, Sedelnikova SE, Rodgers HF, Eadsforth TC, Viner RC, Hawkes TR, Baker PJ & Rice DW (2015) Crystal Structures Reveal that the Reaction Mechanism of Imidazoleglycerol-Phosphate Dehydratase Is Controlled by Switching Mn(II) Coordination. Structure, 23(7), 1236-1245. View this article in WRRO
Zhang R, Zeng X, Kim B, Bushby RJ, Shin K, Baker PJ, Percec V, Leowanawat P & Ungar G (2015) Columnar Liquid Crystals in Cylindrical Nanoconfinement. ACS Nano, 9(2), 1759-1766. View this article in WRRO
Almourfi FM, Rodgers HF, Sedelnikova SE & Baker PJ (2015) Crystallization and preliminary crystallographic analysis of the putative sugar-binding protein Msmeg_0515 (AgaE) fromMycobacterium smegmatis. Acta Crystallographica Section F Structural Biology Communications, 71(2), 189-193.
Esclapez J, Baker P, Rice DW, Pire C, Ferrer J & Bonete MJ (2014) Study of zinc protein ligands in a halophilic enzyme. Current Topics in Peptide and Protein Research, 15, 91-98.
Ramly NZ, Rouzheinikov SN, Sedelnikova SE, Baker PJ, Chow YP, Wan KL, Nathan S & Rice DW (2013) Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 69(Pt 12), 1380-1383.
Abd Aziz AG, Sedelnikova SE, Ruzheinikov SN, Thorpe S, Mohamed R, Nathan S, Rafferty JB, Baker PJ & Rice DW (2012) Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator, BPSL0128, from Burkholderia pseudomallei.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 68(Pt 8), 917-922.
Abd Aziz AG, Ruzheinikov SN, Sedelnikova SE, Mohamed R, Nathan S, Baker PJ & Rice DW (2012) Cloning, purification, crystallization and preliminary X-ray analysis of the Burkholderia pseudomallei L1 ribosomal protein.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 68(Pt 3), 347-350.
Cruz-Migoni A, Ruzheinikov SN, Sedelnikova SE, Obeng B, Chieng S, Mohamed R, Nathan S, Baker PJ & Rice DW (2011) Cloning, purification and crystallographic analysis of a hypothetical protein, BPSL1549, from Burkholderia pseudomallei.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 67(Pt 12), 1623-1626.
Cruz-Migoni A, Hautbergue GM, Artymiuk PJ, Baker PJ, Bokori-Brown M, Chang C-T, Dickman MJ, Essex-Lopresti A, Harding SV, Mahadi NM , Marshall LE et al (2011) A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.. Science, 334(6057), 821-824. View this article in WRRO
Tomlinson JH, Green VL, Baker PJ & Williamson MP (2010) Structural origins of pH-dependent chemical shifts in the B1 domain of protein G.. Proteins, 78(14), 3000-3016.
Domenech J, Baker PJ, Sedelnikova SE, Rodgers HF, Rice DW & Ferrer J (2009) Crystallization and preliminary X-ray analysis of D-2-hydroxyacid dehydrogenase from Haloferax mediterranei.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 65(Pt 4), 415-418.
Baker PJ, Britton KL, Fisher M, Esclapez J, Pire C, Bonete MJ, Ferrer J & Rice DW (2009) Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily.. Proc Natl Acad Sci U S A, 106(3), 779-784.
Haydon DJ, Stokes NR, Ure R, Galbraith G, Bennett JM, Brown DR, Baker PJ, Barynin VV, Rice DW, Sedelnikova SE , Heal JR et al (2008) An inhibitor of FtsZ with potent and selective anti-staphylococcal activity.. Science, 321(5896), 1673-1675.
Davies CL, Ruzheinikov SN, Sedelnikova SE, Baker PJ & Rice DW (2008) Molecular basis for inhibition and resistance of glutamate racemase to a family of D-glu analogues. Acta Crystallographica Section A Foundations of Crystallography, 64(a1), C349-C349.
Xu L, Sedelnikova SE, Baker PJ & Rice DW (2008) Cloning, purification and preliminary crystallographic analysis of a putative DNA-binding membrane protein, YmfM, from Staphylococcus aureus.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 64(Pt 7), 656-658.
Xu L, Sedelnikova SE, Baker PJ, Hunt A, Errington J & Rice DW (2007) Crystal structure of S. aureus YlaN, an essential leucine rich protein involved in the control of cell shape.. Proteins, 68(2), 438-445.
Macmaster R, Sedelnikova S, Baker PJ, Bolt EL, Lloyd RG & Rafferty JB (2006) RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity.. Nucleic Acids Res, 34(19), 5577-5584. View this article in WRRO
Xu L, Sedelnikova SE, Baker PJ & Rice DW (2006) Cloning, purification and preliminary crystallographic analysis of a conserved hypothetical protein, SA0961 (YlaN), from Staphylococcus aureus.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 62(Pt 8), 778-780. View this article in WRRO
Berrisford JM, Hounslow AM, Akerboom J, Hagen WR, Brouns SJJ, van der Oost J, Murray IA, Michael Blackburn G, Waltho JP, Rice DW & Baker PJ (2006) Evidence supporting a cis-enediol-based mechanism for Pyrococcus furiosus phosphoglucose isomerase.. J Mol Biol, 358(5), 1353-1366.
Britton KL, Baker PJ, Fisher M, Ruzheinikov S, Gilmour DJ, Bonete M-J, Ferrer J, Pire C, Esclapez J & Rice DW (2006) Analysis of protein solvent interactions in glucose dehydrogenase from the extreme halophile Haloferax mediterranei.. Proc Natl Acad Sci U S A, 103(13), 4846-4851.
Glynn SE, Baker PJ, Sedelnikova SE, Davies CL, Eadsforth TC, Levy CW, Rodgers HF, Blackburn GM, Hawkes TR, Viner R & Rice DW (2005) Structure and mechanism of imidazoleglycerol-phosphate dehydratase.. Structure, 13(12), 1809-1817.
Ruzheinikov SN, Taal MA, Sedelnikova SE, Baker PJ & Rice DW (2005) Substrate-induced conformational changes in Bacillus subtilis glutamate racemase and their implications for drug discovery.. Structure, 13(11), 1707-1713.
Esclapez J, Britton KL, Baker PJ, Fisher M, Pire C, Ferrer J, Bonete MJ & Rice DW (2005) Crystallization and preliminary X-ray analysis of binary and ternary complexes of Haloferax mediterranei glucose dehydrogenase. ACTA CRYSTALLOGR F, 61, 743-746.
Glynn SE, Baker PJ, Sedelnikova SE, Levy CW, Rodgers HF, Blank J, Hawkes TR & Rice DW (2005) Purification, crystallization and preliminary crystallographic analysis of Arabidopsis thaliana imidazoleglycerol-phosphate dehydratase.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 61(Pt 8), 776-778.
Taal MA, Sedelnikova SE, Ruzheinikov SN, Baker PJ & Rice DW (2004) Expression, purification and preliminary X-ray analysis of crystals of Bacillus subtilis glutamate racemase.. Acta Crystallogr D Biol Crystallogr, 60(Pt 11), 2031-2034.
Berrisford JM, Akerboom J, Brouns S, Sedelnikova SE, Turnbull AP, van der Oost J, Salmon L, Hardré R, Murray IA, Blackburn GM , Rice DW et al (2004) The structures of inhibitor complexes of Pyrococcus furiosus phosphoglucose isomerase provide insights into substrate binding and catalysis.. J Mol Biol, 343(3), 649-657.
Ruzheinikov SN, Das SK, Sedelnikova SE, Baker PJ, Artymiuk PJ, García-Lara J, Foster SJ & Rice DW (2004) Analysis of the open and closed conformations of the GTP-binding protein YsxC from Bacillus subtilis.. J Mol Biol, 339(2), 265-278.
Baker PJ (2004) From hyperthermophiles to psychrophiles: the structural basis of temperature stability of the amino acid dehydrogenases.. Biochem Soc Trans, 32(Pt 2), 264-268.
Hartley A, Glynn SE, Barynin V, Baker PJ, Sedelnikova SE, Verhees C, de Geus D, van der Oost J, Timson DJ, Reece RJ & Rice DW (2004) Substrate specificity and mechanism from the structure of Pyrococcus furiosus galactokinase.. J Mol Biol, 337(2), 387-398.
Das SK, Sedelnikova SE, Baker PJ, Ruzheinikov SN, Foster SJ & Rice DW (2004) Expression, purification, crystallization and preliminary crystallographic analysis of a putative GTP-binding protein, YsxC, from Bacillus subtilis.. Acta Crystallogr D Biol Crystallogr, 60(Pt 1), 166-168.
Rafferty JB, Bolt EL, Muranova TA, Sedelnikova SE, Leonard P, Pasquo A, Baker PJ, Rice DW, Sharples GJ & Lloyd RG (2003) The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold.. Structure, 11(12), 1557-1567.
Dall'Antonia F, Baker PJ & Schneider TR (2003) Optimization of selenium substructures as obtained from SHELXD. ACTA CRYSTALLOGR D, 59, 1987-1994.
de Geus D, Hartley AP, Sedelnikova SE, Glynn SE, Baker PJ, Verhees CH, van der Oost J & Rice DW (2003) Cloning, purification, crystallization and preliminary crystallographic analysis of galactokinase from Pyrococcus furiosus.. Acta Crystallogr D Biol Crystallogr, 59(Pt 10), 1819-1821.
Akerboom J, Turnbull AP, Hargreaves D, Fisher M, de Geus D, Sedelnikova SE, Berrisford JM, Baker PJ, Verhees CH, van der Oost J & Rice DW (2003) Purification, crystallization and preliminary crystallographic analysis of phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus.. Acta Crystallogr D Biol Crystallogr, 59(Pt 10), 1822-1823.
Berrisford JM, Akerboom J, Turnbull AP, de Geus D, Sedelnikova SE, Staton I, McLeod CW, Verhees CH, van der Oost J, Rice DW & Baker PJ (2003) Crystal structure of Pyrococcus furiosus phosphoglucose isomerase. Implications for substrate binding and catalysis.. J Biol Chem, 278(35), 33290-33297.
Slater NJ, Yamaguchi M, Rothwell DG, Baker P, Heyworth CM & Chopra R (2003) The human granulocyte/macrophage colony-stimulating factor receptor α2 isoform influences haemopoietic lineage commitment and divergence. British Journal of Haematology, 122(1), 150-158.
Irwin JA, Lynch SV, Coughlan S, Baker PJ, Gudmundsson HM, Alfredsson GA, Rice DW & Engel PC (2003) Alanine dehydrogenase from the psychrophilic bacterium strain PA-43: overexpression, molecular characterization, and sequence analysis. EXTREMOPHILES, 7(2), 135-143.
Aunpad R, Muench SP, Baker PJ, Sedelnikova S, Panbangred W, Doukyu N, Aono R & Rice DW (2002) Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin.. Acta Crystallogr D Biol Crystallogr, 58(Pt 12), 2182-2183.
Mehanni MM, Turnbull AP, Sedelnikova SE, Baker PJ, Foster S & Rice DW (2002) Crystallization and preliminary X-ray analysis of the ytxM gene product from Bacillus subtilis.. Acta Crystallogr D Biol Crystallogr, 58(Pt 12), 2138-2140.
Muranova TA, Ruzheinikov SN, Sedelnikova SE, Baker PJ, Pasquo A, Galkin A, Esaki N, Ohshima T, Soda K & Rice DW (2002) Crystallization and preliminary X-ray analysis of substrate complexes of leucine dehydrogenase from Thermoactinomyces intermedius.. Acta Crystallogr D Biol Crystallogr, 58(Pt 6 Pt 2), 1059-1062.
Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Slabas AR & Rafferty JB (2002) X-ray crystallographic studies on butyryl-ACP reveal flexibility of the structure around a putative acyl chain binding site.. Structure, 10(6), 825-835.
Bailey S, Sedelnikova SE, Blackburn GM, Abdelghany HM, Baker PJ, McLennan AG & Rafferty JB (2002) The crystal structure of diadenosine tetraphosphate hydrolase from Caenorhabditis elegans in free and binary complex forms.. Structure, 10(4), 589-600.
Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Rafferty JB & Slabas AR (2002) Crystallization and preliminary X-ray crystallographic studies on acyl-(acyl carrier protein) from Escherichia coli.. Acta Crystallogr D Biol Crystallogr, 58(Pt 2), 330-332.
Levy CW, Buckley PA, Sedelnikova S, Kato Y, Asano Y, Rice DW & Baker PJ (2002) Insights into enzyme evolution revealed by the structure of methylaspartate ammonia lyase.. Structure, 10(1), 105-113.
Ferrer J, Fisher M, Burke J, Sedelnikova SE, Baker PJ, Gilmour DJ, Bonete MJ, Pire C, Esclapez J & Rice DW (2001) Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Haloferax mediterranei.. Acta Crystallogr D Biol Crystallogr, 57(Pt 12), 1887-1889.
Levy CW, Buckley PA, Baker PJ, Sedelnikova S, Rodgers F, Li YF, Kato Y, Asano Y & Rice DW (2001) Crystallization and preliminary X-ray analysis of Citrobacter amalonaticus methylaspartate ammonia lyase.. Acta Crystallogr D Biol Crystallogr, 57(Pt 12), 1922-1924.
Ruzheinikov SN, Das SK, Sedelnikova SE, Hartley A, Foster SJ, Horsburgh MJ, Cox AG, McCleod CW, Mekhalfia A, Blackburn GM , Rice DW et al (2001) The 1.2 A structure of a novel quorum-sensing protein, Bacillus subtilis LuxS.. J Mol Biol, 313(1), 111-122.
Roujeinikova A, Raasch C, Burke J, Baker PJ, Liebl W & Rice DW (2001) The crystal structure of Thermotoga maritima maltosyltransferase and its implications for the molecular basis of the novel transfer specificity.. J Mol Biol, 312(1), 119-131.
Ruzheinikov SN, Burke J, Sedelnikova S, Baker PJ, Taylor R, Bullough PA, Muir NM, Gore MG & Rice DW (2001) Glycerol dehydrogenase. structure, specificity, and mechanism of a family III polyol dehydrogenase.. Structure, 9(9), 789-802.
Britton KL, Abeysinghe IS, Baker PJ, Barynin V, Diehl P, Langridge SJ, McFadden BA, Sedelnikova SE, Stillman TJ, Weeradechapon K & Rice DW (2001) The structure and domain organization of Escherichia coli isocitrate lyase.. Acta Crystallogr D Biol Crystallogr, 57(Pt 9), 1209-1218.
Das SK, Sedelnikova SE, Baker PJ, Ruzheinikov SN, Foster S, Hartley A, Horsburgh MJ & Rice DW (2001) Cloning, purification, crystallization and preliminary crystallographic analysis of Bacillus subtilis LuxS.. Acta Crystallogr D Biol Crystallogr, 57(Pt 9), 1324-1325.
Clements JM, Beckett RP, Brown A, Catlin G, Lobell M, Palan S, Thomas W, Whittaker M, Wood S, Salama S , Baker PJ et al (2001) Antibiotic activity and characterization of BB-3497, a novel peptide deformylase inhibitor. ANTIMICROB AGENTS CH, 45(2), 563-570.
Burke J, Ruzheinikov SN, Sedelnikova S, Baker PJ, Holmes D, Muir NM, Gore MG & Rice DW (2001) Purification, crystallization and quaternary structure analysis of a glycerol dehydrogenase S305C mutant from Bacillus stearothermophilus.. Acta Crystallogr D Biol Crystallogr, 57(Pt 1), 165-167.
Ruzheinikov SN, Sedelnikova S, Burke J, Baker PJ, Gore M & Rice DW (2000) The mechanism of the oxidation of glycerol to dihydroxyacetone in Bacillus stearothermophilus. Biochemical Society Transactions, 28(5), A331-A331.
Sedelnikova SE, Burke J, Buckley PA, Rice DW, Jackson JB, Cotton NP, Grimley RL & Baker PJ (2000) Crystallization of the dI component of transhydrogenase, a proton-translocating membrane protein.. Acta Crystallogr D Biol Crystallogr, 56(Pt 9), 1170-1172.
Buckley PA, Baz Jackson J, Schneider T, White SA, Rice DW & Baker PJ (2000) Protein-protein recognition, hydride transfer and proton pumping in the transhydrogenase complex.. Structure, 8(8), 809-815.
Burke J, Roujeinikova A, Baker PJ, Sedelnikova S, Raasch C, Liebl W & Rice DW (2000) Crystallization and preliminary X-ray crystallographic studies on maltosyltransferase from Thermotoga maritima.. Acta Crystallogr D Biol Crystallogr, 56(Pt 8), 1049-1050.
Britton K, Langridge S, Baker PJ, Weeradechapon K, Sedelnikova SE, De Lucas JR, Rice DW & Turner G (2000) The crystal structure and active site location of isocitrate lyase from the fungus Aspergillus nidulans.. Structure, 8(4), 349-362.
Roujeinikova A, Levy CW, Rowsell S, Sedelnikova S, Baker PJ, Minshull CA, Mistry A, Colls JG, Camble R, Stuitje AR , Slabas AR et al (1999) Crystallographic analysis of triclosan bound to enoyl reductase.. J Mol Biol, 294(2), 527-535.
Britton KL, Yip KS, Sedelnikova SE, Stillman TJ, Adams MW, Ma K, Maeder DL, Robb FT, Tolliday N, Vetriani C , Rice DW et al (1999) Structure determination of the glutamate dehydrogenase from the hyperthermophile Thermococcus litoralis and its comparison with that from Pyrococcus furiosus.. J Mol Biol, 293(5), 1121-1132.
Levy CW, Roujeinikova A, Sedelnikova S, Baker PJ, Stuitje AR, Slabas AR, Rice DW & Rafferty JB (1999) Molecular basis of triclosan activity. NATURE, 398(6726), 383-384.
Stillman TJ, Migueis AM, Wang XG, Baker PJ, Britton KL, Engel PC & Rice DW (1999) Insights into the mechanism of domain closure and substrate specificity of glutamate dehydrogenase from Clostridium symbiosum.. J Mol Biol, 285(2), 875-885.
Baker PJ, Sawa Y, Shibata H, Sedelnikova SE & Rice DW (1998) Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase.. Nat Struct Biol, 5(7), 561-567.
Sedelnikova S, Rice DW, Shibata H, Sawa Y & Baker PJ (1998) Crystallization of the alanine dehydrogenase from Phormidium lapideum.. Acta Crystallogr D Biol Crystallogr, 54(Pt 3), 407-408.
Pasquo A, Britton KL, Baker PJ, Brearley G, Hinton RJ, Moir AJ, Stillman TJ & Rice DW (1998) Crystallization of NAD+-dependent phenylalanine dehydrogenase from Nocardia sp239.. Acta Crystallogr D Biol Crystallogr, 54(Pt 2), 269-272.
Baker PJ, Waugh ML, Wang XG, Stillman TJ, Turnbull AP, Engel PC & Rice DW (1997) Determinants of substrate specificity in the superfamily of amino acid dehydrogenases.. Biochemistry, 36(51), 16109-16115.
Turnbull AP, Baker PJ & Rice DW (1997) Analysis of the quaternary structure, substrate specificity, and catalytic mechanism of valine dehydrogenase.. J Biol Chem, 272(40), 25105-25111.
Langridge SJ, Baker PJ, DeLucas JR, Sedelnikova SE, Turner G & Rice DW (1997) Isocitrate lyase from Aspergillus nidulans: crystallization and X-ray analysis of a glyoxylate cycle enzyme.. Acta Crystallogr D Biol Crystallogr, 53(Pt 4), 488-490.
Roe SM, Gormal C, Smith BE, Baker P, Rice D, Card G & Lindley P (1997) Crystallization and preliminary X-ray studies of nitrogenase component 1 (the MoFe protein) from Klebsiella pneumoniae. Acta Crystallographica Section D Biological Crystallography, 53(2), 227-228.
Baldock C, Rafferty JB, Sedelnikova SE, Baker PJ, Stuitje AR, Slabas AR, Hawkes TR & Rice DW (1996) A mechanism of drug action revealed by structural studies of enoyl reductase.. Science, 274(5295), 2107-2110.
Rafferty JB, Sedelnikova SE, Hargreaves D, Artymiuk PJ, Baker PJ, Sharples GJ, Mahdi AA, Lloyd RG & Rice DW (1996) Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction.. Science, 274(5286), 415-421.
Yip KS, Stillman TJ, Britton KL, Artymiuk PJ, Baker PJ, Sedelnikova SE, Engel PC, Pasquo A, Chiaraluce R & Consalvi V (1995) The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures.. Structure, 3(11), 1147-1158.
Rafferty JB, Simon JW, Baldock C, Artymiuk PJ, Baker PJ, Stuitje AR, Slabas AR & Rice DW (1995) Common themes in redox chemistry emerge from the X-ray structure of oilseed rape (Brassica napus) enoyl acyl carrier protein reductase.. Structure, 3(9), 927-938.
Nunn RS, Artymiuk PJ, Baker PJ, Rice DW & Hunter CN (1995) Purification and Crystallization of the Light Harvesting LH1 complex fromRhodobacter sphaeroides. Journal of Molecular Biology, 252(1), 153-153.
Wilkinson KW, Baker PJ, Rice DW, Rodgers HF, Stillman TJ, Hawkes T, Thomas P & Edwards L (1995) Crystallization and analysis of the subunit assembly and quaternary structure of imidazoleglycerol phosphate dehydratase from Saccharomyces cerevisiae.. Acta Crystallogr D Biol Crystallogr, 51(Pt 5), 845-847.
Wilkinson KW, Baker PJ, Rice DW, Stillman TJ, Gore MG, Krauss O & Atkinson T (1995) Crystallization of glycerol dehydrogenase from Bacillus stearothermophilus.. Acta Crystallogr D Biol Crystallogr, 51(Pt 5), 830-832.
SEAH SYK, BRITTON KL, BAKER PJ, RICE DW, ASANO Y & ENGEL PC (1995) ALTERATION IN RELATIVE ACTIVITIES OF PHENYLALANINE DEHYDROGENASE TOWARDS DIFFERENT SUBSTRATES BY SITE-DIRECTED MUTAGENESIS. FEBS LETT, 370(1-2), 93-96.
Baker PJ, Turnbull AP, Sedelnikova SE, Stillman TJ & Rice DW (1995) A role for quaternary structure in the substrate specificity of leucine dehydrogenase.. Structure, 3(7), 693-705.
Britton KL, Baker PJ, Borges KM, Engel PC, Pasquo A, Rice DW, Robb FT, Scandurra R, Stillman TJ & Yip KS (1995) Insights into thermal stability from a comparison of the glutamate dehydrogenases from Pyrococcus furiosus and Thermococcus litoralis.. Eur J Biochem, 229(3), 688-695.
Teller JK, Baker PJ, Britton KL, Engel PC, Rice DW & Stillman TJ (1995) Correlation of intron-exon organisation with the three-dimensional structure in glutamate dehydrogenase.. Biochim Biophys Acta, 1247(2), 231-238.
Yip KS, Baker PJ, Britton KL, Engel PC, Rice DW, Sedelnikova SE, Stillman TJ, Pasquo A, Chiaraluce R, Consalvi V & Scandurra R (1995) Crystallization of the NAD(P)-dependent glutamate dehydrogenase from the hyperthermophile Pyrococcus furiosus.. Acta Crystallogr D Biol Crystallogr, 51(Pt 2), 240-242.
Wang XG, Britton KL, Baker PJ, Martin S, Rice DW & Engel PC (1995) Alteration of the amino acid substrate specificity of clostridial glutamate dehydrogenase by site-directed mutagenesis of an active-site lysine residue.. Protein Eng, 8(2), 147-152.
Henriksen A, Gajhede M, Baker P, Smith AT & Burke JF (1995) Crystallization and preliminary X-ray studies of recombinant horseradish peroxidase. Acta Crystallographica Section D Biological Crystallography, 51(1), 121-123.
Wang XG, Dean JL, Engel PC, Baker PJ, Britton KL, Stillman TJ & Rice DW (1994) The changed pattern of substrate specificity in the K89L mutant of glutamate dehydrogenase of Clostridium symbiosum.. Biochem Soc Trans, 22(3), 320S.
Dean JL, Wang XG, Teller JK, Waugh ML, Britton KL, Baker PJ, Stillman TJ, Martin SR, Rice DW & Engel PC (1994) The catalytic role of aspartate in the active site of glutamate dehydrogenase.. Biochem J, 301 ( Pt 1), 13-16.
Turnbull AP, Ashford SR, Baker PJ, Rice DW, Rodgers FH, Stillman TJ & Hanson RL (1994) Crystallization and quaternary structure analysis of the NAD(+)-dependent leucine dehydrogenase from Bacillus sphaericus.. J Mol Biol, 236(2), 663-665.
KORBER FCF, RIZKALLAH PJ, ATTWOOD TK, WOOTTON JC, MCPHERSON MJ, NORTH ACT, GEDDES AJ, ABEYSINGHE ISB, BAKER PJ, DEAN JLE , ENGEL PC et al (1993) CRYSTALLIZATION OF THE NADP+-DEPENDENT GLUTAMATE-DEHYDROGENASE FROM ESCHERICHIA-COLI. J MOL BIOL, 234(4), 1270-1273.
Britton KL, Baker PJ, Engel PC, Rice DW & Stillman TJ (1993) Evolution of substrate diversity in the superfamily of amino acid dehydrogenases. Prospects for rational chiral synthesis.. J Mol Biol, 234(4), 938-945.
Stillman TJ, Baker PJ, Britton KL & Rice DW (1993) Conformational flexibility in glutamate dehydrogenase. Role of water in substrate recognition and catalysis.. J Mol Biol, 234(4), 1131-1139.
BAKER PJ, BRITTON KL, RICE DW, ROB A & STILLMAN TJ (1993) STRUCTURAL CONSEQUENCES OF SEQUENCE PATTERNS IN THE FINGERPRINT REGION OF THE NUCLEOTIDE-BINDING FOLD (VOL 228, PG 662, 1992). JOURNAL OF MOLECULAR BIOLOGY, 232(3), 1012-1012.
Baker PJ, Britton KL, Rice DW, Rob A & Stillman TJ (1993) Erratum: Structural consequences of sequence patterns in the fingerprint region of the nucleotide binding fold (J. Mol. Biol. (1992) 228, 662-671). Journal of Molecular Biology, 232(3), 1012.
Nunn RS, Artymiuk PJ, Baker PJ, Rice DW & Hunter CN (1992) Purification and crystallization of the light harvesting LH1 complex from Rhodobacter sphaeroides.. J Mol Biol, 228(4), 1259-1262.
Baker PJ, Britton KL, Rice DW, Rob A & Stillman TJ (1992) Structural consequences of sequence patterns in the fingerprint region of the nucleotide binding fold. Implications for nucleotide specificity.. J Mol Biol, 228(2), 662-671.
Britton KL, Baker PJ, Rice DW & Stillman TJ (1992) Structural relationship between the hexameric and tetrameric family of glutamate dehydrogenases.. Eur J Biochem, 209(3), 851-859.
Stillman TJ, Baker PJ, Britton KL, Rice DW & Rodgers HF (1992) Effect of additives on the crystallization of glutamate dehydrogenase from Clostridium symbiosum. Evidence for a ligand-induced conformational change.. J Mol Biol, 224(4), 1181-1184.
Baker PJ, Britton KL, Engel PC, Farrants GW, Lilley KS, Rice DW & Stillman TJ (1992) Subunit assembly and active site location in the structure of glutamate dehydrogenase.. Proteins, 12(1), 75-86.
Lilley KS, Baker PJ, Britton KL, Stillman TJ, Brown PE, Moir AJ, Engel PC, Rice DW, Bell JE & Bell E (1991) The partial amino acid sequence of the NAD(+)-dependent glutamate dehydrogenase of Clostridium symbiosum: implications for the evolution and structural basis of coenzyme specificity.. Biochim Biophys Acta, 1080(3), 191-197.
Abeysinghe SI, Baker PJ, Rice DW, Rodgers HF, Stillman TJ, Ko YH, McFadden BA & Nimmo HG (1991) Use of chemical modification in the crystallization of isocitrate lyase from Escherichia coli.. J Mol Biol, 220(1), 13-16.
BAKER PJ, FARRANTS GW, STILLMAN TJ, BRITTON KL, HELLIWELL JR & RICE DW (1990) ISOMORPHOUS REPLACEMENT WITH OPTIMIZED ANOMALOUS SCATTERING APPLIED TO PROTEIN CRYSTALLOGRAPHY. ACTA CRYSTALLOGR A, 46, 721-725.
Baker PJ, Farrants GW, Rice DW & Stillman TJ (1987) Recent progress on the structure and function of glutamate dehydrogenase.. Biochem Soc Trans, 15(4), 748-751.
STILLMAN TJ, BAKER PJ, RODGERS HF & RICE DW (1987) INTERPRETATION OF THE 2.5 A RESOLUTION CRYSTAL-STRUCTURE OF GDH. PROTEIN ENG, 1(3), 248-248.
Rice DW, Baker PJ, Farrants GW & Hornby DP (1987) The crystal structure of glutamate dehydrogenase from Clostridium symbiosum at 0.6 nm resolution.. Biochem J, 242(3), 789-795.
BAKER PJ, THOMAS DH, BARTON CH, RICE DW & BAILEY E (1987) CRYSTALLIZATION OF AN NADP+-DEPENDENT MALIC ENZYME FROM RAT-LIVER. J MOL BIOL, 193(1), 233-235.
Rice CA, Campbell SJ, Bisson C, Owen HJ, Sedelnikova SE, Baker PJ, Rice DW, Henriquez FL & Roberts CW () Structural and functional studies of histidine biosynthesis in Acanthamoeba spp. demonstrates a novel molecular arrangement and target for antimicrobials. PLOS ONE, 13(7), e0198827-e0198827. View this article in WRRO

Chapters

Simon JW, Gilroy J, Roujenikova A, Rafferty J, Baldock C, Baker PJ, Rice DW, Stuitje AR & Slabas AR (2003) Determination of the X-Ray Crystallographic Structure of E.coli Butyryl-ACP, Advanced Research on Plant Lipids (pp. 139-142). Springer Netherlands
Bisson C, Baker PJ & Rice DW () Imidazoleglycerol-Phosphate Dehydratase (IGPD) (pp. 1-13). John Wiley & Sons, Ltd

Conference proceedings papers

Asano Y, Kato Y, Levy C, Baker P & Rice D (2004) Structure and Function of Amino Acid Ammonia-lyases. Biocatalysis and Biotransformation, Vol. 22(2) (pp 133-140)
Bailey S, Abdelghany HM, Sedelnikova SE, Blackburn GM, Baker PJ, Rafferty JB & McLennan AG (2002) Crystal structure and mutational analysis of the diadenosine tetraphosphate (Ap4A) 'Nudix' hydrolase from Caenorhabditis elegans. DRUG DEVELOPMENT RESEARCH, Vol. 56(4) (pp 567-567)
Buckley PA, Jackson JB, Rice DW, Sedelnikova SE, Burke J, Shneider T, Roth M & Baker PJ (2000) The Crystallization and structure analysis of the dI Component of Transhydrogenase, a Proton-Translocating Membrane Protein. Acta Crystallographica Section A Foundations of Crystallography, Vol. 56(s1) (pp s270-s270)
Wang XG, Britton LK, Baker PJ, Rice DW & Engel PC (1996) Improving the engineered activity of mutants of clostridial glutamate dehydrogenase towards monocarboxylic substrates: Substitution of Ala163 with glycine. BIOCHEMICAL SOCIETY TRANSACTIONS, Vol. 24(1) (pp S126-S126)

Research group: Structural Biology

Teaching activities

Level 4 modules

MBB403 Extended Research Project
MBB402 Advanced Literature Review

Level 3 modules

MBB340 The Microbiology of Extreme Environments
MBB361 Literature Review
MBB380 Project

Level 2 modules

MBB261 Biochemistry 2 (Module coordinator)
MBB265 Practical Molecular Bioscience 2
MBB266 Biostructures, Energetics and Synthesis

Level 1 modules

MBB161 Biochemistry
MBB165 Practical Molecular Bioscience 1