Dr Christa Walther

School of Biosciences

Senior Experimental Officer – Super-resolution Microscopy

c.walther@sheffield.ac.uk
+44 114 222 2733

Firth Court

Full contact details

Dr Christa Walther
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN

Research interests

Application and implementation of super-resolution imaging (Structured Illumination Microscopy and Single Molecule Localisation Microscopy) together with users of the Wolfson Light Microscopy Facility in a variety of research projects, eg, novel stains for organelles, cell division in bacteria, Weibel Palade bodies formation and secretion, cell division in bacteria, localisation and clustering of tetraspanins in mammalian cells, correlative approach combining STORM with atomic force microscopy (AFM)
Implementation of newly emerging image analysis methods for super-resolution imaging

Publications

Singh H, Sreedharan S, Tiwari R, Walther C, Smythe C, Pramanik SK, Thomas JA & Das A (2018) A Fluorescent Chemodosimeter for Organelle-Specific Imaging of Nucleoside Polyphosphate Dynamics in Living Cells. Crystal Growth and Design, 18(11), 7199-7206. View this article in WRRO
Lund VA, Wacnik K, Turner RD, Cotterell BE, Walther CG, Fenn SJ, Grein F, Wollman AJM, Leake MC, Olivier N , Cadby A et al (2018) Molecular coordination of staphylococcus aureus cell division. eLife, 7. View this article in WRRO
Walther C, Whitfield R & James DC (2015) Importance of Interaction between Integrin and Actin Cytoskeleton in Suspension Adaptation of CHO cells. Applied Biochemistry and Biotechnology, 178(7), 1286-1302.

Walther CG, Hoiczyk E & Lawrence EG (2017) Use of mNeonGreen as label for Single Molecule Localisation Microscopy (SMLM). mmc 2017
Walther CG, Vahldieck M, Robinson D & Foster S (2015) New green emitting fluorochromes for Fluorescence Microscopy, specifically Structured Illumination Microscopy. Microscopy Microscience Congress 2015 (MMC 2015) (pp P2608-P2608). Manchester, 29 June 2015 - 2 July 2015.
Walther CG & Walther T () Sub-diffraction limited imaging of nano-rulers using Structured Illumination Microscopy. Frontiers in BioImaging, 14 July 2016 - 15 July 2016.

Walther CG (2017) Focus in Microscopy 2017
Walther CG & Walther T (2014) Book review of ‘Fundamentals of Fluorescence Microscopy’ by Partha Pratim Mondal and Alberto Diaspro, Springer, Dordrecht, 2014, ISBN: 978-94-007-7544-2

Professional activities and memberships

School of Biosciences