Dr Tom Sheard

School of Biosciences

Research Associate

Tom Sheard
Profile picture of Tom Sheard

Full contact details

Dr Tom Sheard
School of Biosciences
Firth Court
Western Bank
S10 2TN

Career history

  • 2021-present – Postdoctoral research associate, School of Biosciences, University of Sheffield, UK
  • 2016-2021 – PhD, Faculty of Biological Sciences, University of Leeds, UK
  • 2015-2016 – MPhil, Faculty of Biology, Medicine and Health, University of Manchester, UK
Research interests
Super-resolution microscopy imaging techniques allow scientists to see nanoscale structures. These techniques often require specialist equipment and knowledge, rendering them inaccessible to a significant number of scientists in the field.
In my post-doctoral research position I will develop accessible imaging techniques, making use of the novel super-resolution technique called expansion microscopy (ExM), which uses expanding hydrogels to physically inflate a sample to increase the resolution of imaging.
I will innovate the ExM methodology, investigating new gel chemistries, fluorescent probes, and understanding the even-ness (isotropy) of expansion with calibration structures.
I am interested in implementing ExM in novel applications which will benefit from improved resolution to see biological targets such as proteins, lipids and genetic material.

Journal articles


Conference proceedings papers

  • Sheard TMD, Spencer ME, Shakespeare T, Seehra RS, Suen KM & Jayasinghe I (2023) Diverse labelling of cellular compartments with NHS esters in expansion microscopy. Biophysical Journal, Vol. 122(3) (pp 275a-275a) RIS download Bibtex download
  • Diaz YDLMZ, Andersen C, Kure JL, Eriksen MH, Lovatt AL, Lagerholm C, Morales S, Sehayek S, Sheard TMD, Wiseman PW & Arnspang EC (2023) Super resolution techniques combined with image spatial correlation analysis to study Angiotensin II type 1 receptor' dynamic and organization in renal cells. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol. 52(SUPPL 1) (pp S167-S167) RIS download Bibtex download
  • Holmes M, Hurley ME, Sheard TMD, Stevenson-Cocks HJ, Benson A, Jayasinghe I & Colman MA (2020) Sub-cellular Heterogeneity in SERCA Determines Spatial Calcium Dynamics in Cardiomyocytes. BIOPHYSICAL JOURNAL, Vol. 118(3) (pp 172A-173A) RIS download Bibtex download
  • Hurley ME, Sheard TM, Norman R, Kirton HM, Shah SS, Pervolaraki E, Yang Z, Steele DS, Gamper N, White E & Jayasinghe I (2020) Optical Superresolution Analysis of Intracellular Calcium Handling Proteins and Correlating Calcium Signal Morphology. BIOPHYSICAL JOURNAL, Vol. 118(3) (pp 172A-172A) RIS download Bibtex download