Biophysical and Photophysical Chemistry

Our work develops new methods to characterise chemical and biological systems and their behaviour; explores the mechanisms of charge and energy transfer in molecules and material; and develops new analytical methodologies for the characterisation of molecular systems, biomolecules, and materials.

Research themes in the biophysical and photo physical group
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Major Themes

We are a dynamic, interdisciplinary group of people sharing passion for light-driven phenomena and (supra)molecular interactions at the interface of chemistry, physics and biology - collaborators welcome.

  • Ultra-fast processes, light matter interactions: charge and energy transfer in molecular systems and biomolecules, artificial photosynthesis and light-harvesting, photocatalysis, photodynamic therapy
  • Biophysical chemistry: single-molecule spectroscopy and microscopy, energy transfer in molecular systems, repurposing of natural systems for synthetic biology applications, self-assembling proteins and biological membranes, plant signalling
  • Surfaces and materials: plasmonics, surface spectroscopy, bionanotechnology, photonics
  • Applied spectroscopy: development of new analytical spectroscopic techniques and its applications, to study the metabolism of bacteria, and sensing of nutrients in soils.

Methods

Ultrafast laser spectroscopy: transient absorption and time-resolved infrared, 2D-IR, time-correlated single-photon counting, fluorescence upconversion spectroscopy. 

Ultrasensitive techniques: single-molecule FRET, cavity ring-down spectroscopy, Raman spectroscopy, direct singlet oxygen detection.

Imaging techniques: Nanolithography, surface patterning, AFM, Molecular photonics breadboards, Optical Coherence Tomography (OCT), FLIM and phosphorescence lifetime imaging microscopy in cells and tissues.

Protein engineering: rational design of self-assembling proteins and immunofluorescence microscopy.

Key publications

Ambrose, B., Baxter, J.M., Cully, J. et al (2020). The smfBox is an open-source platform for single-molecule FRET. Nat Commun, 11, 5641. 

G.D. Metcalfe, S. Alahmari, T.W. Smith, and M. Hippler (2019). Cavity-Enhanced Raman and Helmholtz Resonator Photoacoustic Spectroscopy to Monitor the Mixed Sugar Metabolism of E. coli. Anal. Chem., 91 13096-13104. 

A. Lishchuk, G. Kodali, J. A. Mancini, M. Broadbent, B. Darroch,O. A. Mass, A. Nabok, P. L. Dutton, C. N. Hunter, P. Törmä and G. J. Leggett (2018). A Synthetic Biological Quantum Optical System. Nanoscale, 10, 13064 - 13073.

A. Graf, J. Finkel, A. Chauvet & S. Rtimi (2019). Deciphering the mechanisms of bacterial inactivation on HiPIMS sputtered CuxO-FeOx-PET surfaces: from light absorption to catalytic bacterial death. Appl. Mater. Interfaces, 11, 48, 45319–45329.

Booth A, Marklew CJ, Ciani B & Beales PA (2019). In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension. iScience, 15, 173-184.

Delor M, Scattergood PA, Sazanovich IV, Parker AW, Greetham GM, Meijer AJHM, Towrie M & Weinstein JA (2014). Toward control of electron transfer in donor-acceptor molecules by bond-specific infrared excitation. Science, 346(6216), 1492-1495.

Key Facilities

Lord Porter Laser Laboratory

Surface Analysis Center

Single-molecule FRET and TCSPC

Collaborations within the University

We are part of many cross-faculty initiatives:

Grantham Center for Sustainable Futures, The Florey Institute, SinFoNia, Imagine: Imaging Life, Centre for Membrane Interactions and Dynamics.

People

For further information about Biophysical and Photophysical Chemistry at Sheffield please see the staff page of individual researchers below.

Professor Julia Weinstein

Dr Tim Craggs

Dr Adrien Chauvet

Dr Barbara Ciani

Dr Michael Hippler

Professor Graham Leggett

Members of other research clusters active in biophysical and photophysical chemistry:

Professor Steve Armes FRS

Professor Jane Grasby

Professor Anthony Meijer

Dr Grant Hill

Dr Ahmed Iraqi

Dr Natalia Martsinovich

Dr Ben Partridge

Dr Peter Portius

Professor Jim Thomas

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