Dr. David Buttle BSc PhD

Emeritus Fellow

Dave ButtleDepartment of Infection, Immunity & Cardiovascular Disease
University of Sheffield
Medical School
Beech Hill Road
Sheffield
S10 2RX
United Kingdom

Tel: +44 (0)114 215 9516
Email: d.j.buttle@sheffield.ac.uk
Fax: +44 (0)114 271 1863

Biography:

I began my career as a technician before obtaining, in 1978, a Research Fellowship at Shriners Burns Institute & Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, U.S.A. From 1982 I held research posts in the Department of Biochemistry, Strangeways Research Laboratory, Cambridge, after which I became a post-doctoral Research Fellow with the Arthritis & Rheumatism Council. In 1993 I was appointed to a Senior Lecturer post at The University of Sheffield Medical School then as Reader in Matrix Biology in 2000. I retired from the University in September 2017.

Research Interests:

My major interest is in proteolytic enzymes and extracellular matrix turnover, with principal funding from Arthritis Research UK, The Wellcome Trust, MRC and the Dunhill Medical Trust.

Professional Activities:

I am a member of the following Learned Societies:

  • The Biochemical Society.
  • British Society for Matrix Biology (formerly British Connective Tissue Society).

Current Projects:

  • Glycosaminoglycans as modifiers of protein localisation and function.
  • Developing new aids for cartilage regeneration.
  • Proteoglycans – functions and turnover.
  • Developing plant cysteine proteinases as anthelmintics.
  • The importance of the stromal compartment in cancer progression.
  • Proteinases in kidney fibrosis.

Key Publications:

  1. Ikpegbu E, Basta L, Clements DN, Fleming R, Vincent TL, Buttle DJ, Pitsillides AA, Staine KA, Farquharson C. (2018) FGF-2 promotes osteocyte differentiation through increase E11/podoplanin expression. J Cell Physiol doi:[10.1002/jcp.26345] In press
  2. Njom VS, Winks T, Diallo O, Lowe A, Behnke J, Dickman MJ, Duce I, Johnstone I, Buttle DJ. The mechanism of attack by plant cysteine proteinases on the nematode cuticle. In preparation.
  3. Buttle DJ, Haycock JW, Crawford A. Exploitation of the glycosaminoglycan (heparin) interactome in the potential repair and regeneration of skeletal tissues. In preparation.
  4. Staines KA, Prideaux M, Allen S, Buttle DJ, Pitsillides AA, Farquharson C. (2016) E11/podoplanin protein stabilization through inhibition of the proteasome promotes osteocyte differentiation in murine in vitro models. J Cell Physiol 231:1392-1401.
  5. Levecke B, Buttle DJ, Behnke JM, Duce IR, Vercruysse J. (2014) Cysteine proteinases from papaya (Carica papaya) in the treatment of experimental Trichuris suis infection in pigs: two randomized controlled trials. Parasites Vectors 7:255.
  6. Robinson DE, Buttle DJ, Short RD, McArthur SL, Steele DA, Whittle JD. (2012) Glycosaminoglycan (GAG) binding surfaces for characterizing GAG-protein interactions. Biomaterials 33:1007-1016.
  7. Buttle DJ, Behnke JM, Bartley Y, Elsheikha HM, Bartley DJ, Garnett MC, Donnan AA, Jackson F, Lowe A, Duce IR. (2011) Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus. Parasites Vectors 4:36.
  8. Pockert AJ, Richardson SM, Le Maitre CL, Lyon M, Deakin JA, Buttle DJ, Freemont AJ, Hoyland JA. (2009) Modified expression of the ADAMTS enzymes and tissue inhibitor of metalloproteinases 3 during human intervertebral disc degeneration. Arthritis Rheum 60:482-491.
  9. Vankemmelbeke MN, Jones GC, Fowles C, Ilic MZ, Handley CJ, Day AJ, Knight CG, Mort JS, Buttle DJ. (2003) Selective inhibition of ADAMTS-1, -4 and -5 by catechin gallate esters. Eur J Biochem 270:2394-2403.
  10. Vankemmelbeke MN, Holen I, Wilson AG, Ilic MZ, Handley CJ, Kelner GS, Clark M, Liu C, Maki RA, Burnett D, Buttle DJ. (2001) Expression and activity of ADAMTS-5 in synovium. Eur J Biochem 268:1259-1268.
  11. Kafienah W, Brömme D, Buttle DJ, Croucher LJ, Hollander AP. (1998) Human cathepsin K cleaves native type I and II collagens at the N-terminal end of the triple helix. Biochem J 331:727-732.
  12. O'Hara BP, Hemmings AM, Buttle DJ, Pearl LH. (1995) Crystal structure of glycyl endopeptidase from Carica papaya: a cysteine endopeptidase of unusual substrate specificity. Biochemistry 34:13190-13195.
  13. Farndale RW, Buttle DJ, Barrett AJ. (1997) Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 883:173-177.
  14. Buttle DJ, Abrahamson M, Burnett D, Mort JS, Barrett AJ, Dando PM, Hill SL. (1991) Human sputum cathepsin B degrades proteoglycan, is inhibited by α2-macroglobulin and is modulated by neutrophil elastase cleavage of cathepsin B precursor and cystatin C. Biochem J 276:325-331.
  15. Buttle DJ, Dando PM, Coe PF, Sharp SL, Shepherd ST, Barrett AJ. (1990) The preparation of fully active chymopapain free of contaminating proteinases. Biol Chem Hoppe-Seyler 371:1083-1088.
  16. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T. (1981) Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. Science 211:1052-1054.
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