Professor Peter Monk BSc PhD
Professor of Immunology
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield
Beech Hill Road
I began my career in the Biochemistry Department in Sheffield in 1988 as a post-doctoral researcher, working for Professor Banks on chemoattractant signalling in monocytes, before working with Dr Birgit Helm on mast cell degranulation. In 1992, I became an independent researcher, funded by Arthritis Research UK to study the important pro-inflammatory mediator, complement fragment 5A (C5a).
In 1995, Arthritis Research UK awarded me a post-doctoral fellowship to establish my own laboratory, working on the interactions between C5a and its receptor. We were the first group to characterise the second C5a receptor, C5L2, now recognised to have important roles in inflammation and neurodegeneration. During this time, I also began work on the tetraspanin family of membrane proteins, identifying the hepatitis C virus E2 glycoprotein binding site on CD81.
I became a lecturer in the Department of Neurosciences in 2001 and was promoted to Senior Lecturer in 2007, before moving to the Department of Infection and Immunity in 2009. I became Reader in Immunology in 2013 and Professor in 2017.
Complement fragment receptors
The complement cascade is a series of blood proteins forming a part of the immune system that responds to infection. Complement protein fragments (C4a, C3a) produced by activation of the cascade actually cause inflammation which is normally a good thing but, when excessively produced, they exacerbate inflammatory diseases and there are no drugs currently available to prevent this. We are investigating the molecular interactions that underlie complement fragment receptor (C5aR, C3aR, C5L2) functions with the aim of developing new drugs for anti-inflammatory therapy.
Tetraspanins have been implicated in many of the fundamental processes involved in the development and functioning of multicellular organisms, from sponges to man. These include cell fusion, migration, secretion, adhesion and signalling. Recently, several tetraspanins have been shown to have roles in microbial infections, including hepatitis C, HIV-1 and Chlamydia. Cell ingress and egress mechanisms involving tetraspanins are exploited by these intracellular pathogens. These mechanisms can be blocked by tetraspanin-specific reagents, suggesting that tetraspanins may be valuable drug targets. However, the molecular details of tetraspanin functions have yet to be fully elucidated. We are using mammalian and zebrafish models to further our understanding of this fascinating protein family.
- MSc Molecular Medicine MED6006 Complement; Allergy and Imunotherapy.
- Preclinical Medicine Innate Immunity and Allergy; Laboratory-based SSC; Student PATS tutor.
- MSc Genomic Medicine MEDT32/33 Complement Genetics.
- MBB311 Molecular Immunology (innate immunity).
- MBB360 Third Year Undergraduate projects.
- MBB460 Fourth Year Undergraduate Projects.
- Member of British Society for Immunology; Biochemical Society, British Inflammation Research Association.
- INSERM scientific evaluation Infection and Immunity panel member.
- Co-organiser for international research conferences on tetraspanins.
- Associate editor for BMC Immunology, Frontiers of Immunology.
- Industry Consultancies.
- Analysis of the C5a receptor ligand binding site.
- Control of C5a function by the second C5a receptor, C5aR2.
- Development of C3aR and C5aR2 antagonists.
- Tetraspanin function in monocyte giant cell formation.
- Tetraspanins and intracellular pathogens.
For key publications see below. For a full list of publications click here.
- Ventress JK, Partridge LJ, Cozens D, Read RC, MacNeil S & Monk PN (2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes. PLoS One, 11(7). View this article in WRRO
- Croker DE, Monk PN, Halai R, Kaeslin G, Schofield Z, Wu MCL, Clark RJ, Blaskovich MAT, Morikis D, Floudas CA, Cooper MA & Woodruff TM (2016) Discovery of functionally selective C5aR2 ligands: novel modulators of C5a signaling. Immunology and Cell Biology, 94, 787-795. View this article in WRRO
- Halai R, Bellows-Peterson ML, Branchett W, Smadbeck J, Kieslich CA, Croker DE, Cooper MA, Morikis D, Woodruff TM, Floudas CA & Monk PN (2014) Derivation of ligands for the complement C3a receptor from the C-terminus of C5a. European Journal of Pharmacology, 745, 176-181. View this article in WRRO
- Cain SA & Monk PN (2002) The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg(74).. J Biol Chem, 277(9), 7165-7169.