Case Study Conference Presentations


Characterizing the microcirculation of atopic dermatitis using angiographic optical coherence tomography

Robust measurements of subclinical inflammation in skin affected by atopic dermatitis using angiogra67th Annual meeting of the British Microcirculation Society 2017, Birmingham, UK

Background: The microcirculation within localised skin lesions often presents a unique morphology when compared to that of healthy skin. In the case of inflammatory conditions such as atopic dermatitis (AD), epidermal thickening is likely to influence both the depth and shape of the underlying vessels. Optical coherence tomography (OCT) provides a non-invasive view into the tissue, however structural measures of epidermal hyperplasia (thickening) are made challenging due to the lack of a delineated dermal-epidermal junction in AD patients. Instead, angiographic extensions to OCT may allow for direct measurement of vascular depth, potentially presenting a more robust method of estimating the degree of subclinical inflammation.

POSTER - Characterizing the microcirculation of atopic dermatitis using angiographic optical coherence tomography (pdf 3.9mb)

ABSTRACT - Characterizing the microcirculation of atopic dermatitis using angiographic optical coherence tomography (.docx 16kb)


Innovating imaging techniques to understand Atopic Dermatitis skin

9th Georg Rajka International Symposium on AD 2016, Sao Paulo, Brazil

Innovating imaging techniques to understand Atopic Dermatitis skin 9th Georg Rajka International SymBackground: Atopic dermatitis (AD) is a chronic relapsing condition that is commonly observed in primary care. The characteristics of such a condition is itching and redness of the skin, associated with breakdown of the skin barrier. Efforts to estimate the prevalence of AD are complicated due to the presence of few studies in the literature. Optical coherence tomography (OCT) provides a fast and non-invasive method of studying the skin barrier and the effects of treatments on it.

POSTER - Innovating imaging techniques to understand Atopic Dermatitis skin (pdf 467kb)

ABSTRACT - Innovating imaging techniques to understand Atopic Dermatitis skin (.doc 14kb)


An investigation of protease activity at non-lesional sites in atopic dermatitis

British Society for Investigative Dermatology annual meeting 2017, Manchester, UK

An investigation of protease activity at nonlesional sites in Atopic DermatitisBackground: Sustained protease hyperactivity within the stratum corneum (SC) is a feature of chronic skin disorders such as Atopic Dermatitis (AD). Animal models have provided a rich source of mechanistic evidence for protease-associated barrier breakdown in AD and related skin diseases, but to date, there is the requirement for further clinical studies in patients. To address this we have developed a simple, non-invasive assay allowing the quantification of a broad-spectrum of proteases at the skin surface. Using this assay we recently identified a subset of neonates at risk of developing AD, with desquamatory protease hyperactivity in normal appearing skin at birth. Here, to extend these findings, a cohort study was designed to investigate protease activity in non-lesional adult skin.

POSTER - An investigation of protease activity at nonlesional sites in Atopic Dermatitis (pdf 639kb)

ABSTRACT - An investigation of protease activity at nonlesional sites in Atopic Dermatitis (.doc 14kb)


Abnormal corneodesmosome distribution in atopic dermatitis and soap-induced xerosis

15th Skin Forum annual meeting 2016, London, UK

Abnormal corneodesmosome distribution in atopic dermatitis and soap-induced xerosisBackground: In the stratum corneum (SC), inter-corneocyte cohesion is provided by corneodesmosomes. In healthy skin, corneodesmosomes are distributed uniformly across the corneocyte surface in the basal SC layers, becoming degraded as the corneocyte matures, leaving only peripheral corneodesmosomes at the SC surface. This gradual loss of corneocyte cohesion is regulated by numerous SC proteases to facilitate desquamation. In atopic dermatitis and soap-induced xerosis, there is abnormal corneodesmosome degradation.

POSTER - Abnormal corneodesmosome distribution in atopic dermatitis and soap-induced xerosis (pdf 865kb)

ABSTRACT - Abnormal corneodesmosome distribution in atopic dermatitis and soap-induced xerosis (.doc 14kb)


Characterisation of the skin barrier defect in atopic dermatitis using in vivo ATR-FTIR molecular spectroscopy

9th Georg Rajka International Symposium on AD 2016, Sao Paulo, Brazil.

Characterisation of the skin barrier defect in atopic dermatitis using in vivo ATR-FTIR molecular spBackground: Attenuated total reflectance (ATR)-Fourier transform infrared (FTIR) spectroscopy is a molecular spectroscopic technique that can be used to investigate the surface properties of human skin in vivo.

POSTER - Characterisation of the skin barrier defect in atopic dermatitis using in vivo ATR-FTIR molecular spectroscopy (pdf 1506kb)

ABSTRACT - Characterisation of the skin barrier defect in atopic dermatitis using in vivo ATR-FTIR molecular spectroscopy (.doc 23kb)


The neonatal epidermal barrier: development of superficial chymotrypsin-like protease activity and natural moisturising factors

9th Georg Rajka International Symposium on AD 2016, Sao Paulo, Brazil.

The neonatal epidermal barrier: development of superficial chymotrypsin-like protease activity and nBackground: The infant stratum corneum (SC) is thinner, and immature in both structure and function compared to adults throughout the first year of life. This vulnerable period coincides with the onset (<1 year of age) of skin manifestations such as atopic dermatitis. From birth, the SC enters a maturation phase of rapid hydration and acid mantle formation. Considering the reported homeostatic action of these functional properties, the observations suggest underdeveloped or transitional mechanisms underlying neonatal epidermal barrier differentiation and desquamation.

POSTER - The neonatal epidermal barrier: development of superficial chymotrypsin-like protease activity and natural moisturising factors (pdf 602kb)

ABSTRACT - The neonatal epidermal barrier: development of superficial chymotrypsin-like protease activity and natural moisturising factors (.doc 14kb)


The effect of a humectant emollient cream containing 5% urea compared to a non-humectant emollient on the skin barrier in older people with dry skin

23rd World Congress of Dermatology 2015, Vancouver, Canada

The effect of a humectant emollient cream containing 5% urea compared to a non-humectant emollient oBackground: Xerosis affects between 30 and 75% of older people. The development of xerotic skin conditions, such as atopic dermatitis, asteatotic eczema and winter xerosis, is associated with a skin barrier defect. This defect is characterised by reduced levels of natural moisturising factor (a collection of natural humectants including urea, pyrrolidone carboxylic acid [PCA], and lactate), abnormal intercellular lipid levels in the stratum corneum (SC), and elevated skin surface pH (linked to increased skin barrier breakdown). Emollients are widely used to treat xerosis, however there is limited mechanistic evidence of their effects on the skin barrier.

POSTER - The effect of a humectant emollient cream containing 5% urea compared to a non-humectant emollient on the skin barrier in older people with dry skin (pdf 667kb)

ABSTRACT - The effect of a humectant emollient cream containing 5% urea compared to a non-humectant emollient on the skin barrier in older people with dry skin (.doc 14kb)


Comparing the effect of tacrolimus (0.1%) ointment and betamethasone (0.1%) valerate on the epidermal barrier: a twice-weekly maintenance dose

23rd World Congress of Dermatology 2015, Vancouver, Canada

Comparing the effect of tacrolimus (0.1%) ointment and betamethasone valerate (0.1%) cream on the epBackground: The proactive use of topical anti-inflammatories is an effective method of addressing the subclinical inflammation associated with the remission phase of atopic dermatitis (AD). To date, the interaction of this treatment dose with the subclinical epidermal barrier defect in AD is yet to be determined.

POSTER - Comparing the effect of tacrolimus (0.1%) ointment and betamethasone (0.1%) valerate on the epidermal barrier: a twice-weekly maintenance dose (pdf 514kb)

ABSTRACT - Comparing the effect of tacrolimus (0.1%) ointment and betamethasone (0.1%) valerate on the epidermal barrier: a twice-weekly maintenance dose (.doc 14kb)