Dr Nicola GreenDr Nicola Green

Research Fellow in Materials Science and Engineering
PhD Biochemistry (University of Kent)

Tel: 0114 2223932
Email: n.h.green@sheffield.ac.uk
Address: Kroto Research Institute, North Campus, University of Sheffield, Broad Lane, Sheffield S3 7HQ

Nicola is a Research Fellow in Materials Science and Engineering. She joined the department in 2006 as a postdoctoral researcher, working with Prof Sheila MacNeil on the development of a tissue engineered model of the human oesophageal mucosa. She obtained her first degree from the University of Nottingham and her PhD from the University of Kent. She has worked as a Research Fellow at the University of Nottingham, in the Laboratory of Biophysics and Surface Analysis, and as a Senior Scientist in a Nottingham University spin-off company. Since 2010 she has also been in charge of the Kroto Imaging Facility.

Nicola’s interdisciplinary research focuses on tissue engineering of soft tissues, with particular emphasis upon the oeosphagus, for both translation to the clinic and as more biologically-relevant experimental models for use in the laboratory. She also has interests in developing novel imaging methods and applying established imaging techniques to the field of tissue engineering, for improved characterization of cell growth and behaviour in 3D cultures throughout the culture period. Current research projects include the use of novel DNA probes for imaging cells and the use of second harmonic generation imaging collagen formation in tissue engineered constructs.

Further Information

Key Projects
  • Developing and using a tissue engineered oesophageal model for the study of Barrett’s Oesophagus and oesophageal adenocarcinoma
  • Development of an oesophageal replacement for clinical use
  • Imaging of biomaterials and tissue engineered constructs
Group Members


Caroline Glover
Marwah A Alsharif

Research Interests
  • Oeosphageal cancer/ Barrett’s Oeosphagus
  • Tissue Engineering
  • Bioimaging
External Links

Confocal Imaging at the Kroto Institute

Nicola's Research Gate profile

Nicola's LinkedIn Profile

Find Nicola on Twitter

  1. Bostan, L.E., Noble, C., Smulders, N., Lewis, R., Carré, M.J., Franklin, S., Green, N.H., MacNeil, S. Measurement of Friction-induced Changes in Pig Aorta Fibre Organization by Non-invasive Imaging as a Model for Detecting the Tissue Response to Endovascular Catheters, Biotribology, 2017.
  2. Oxygen Mapping of Melanoma Spheroids using a Small Molecule Platinum Probe and Phosphorescence Lifetime Imaging Microscopy, Raza, A, Colley, H.E, Baggaley, E., Sazanovich, I.V., Green, N.H., Weinstein, J.A., Botchway, S.W., MacNeil, S.1, Haycock, J.W. – accepted by Scientific Reports
  3. Cox-Nowak, K., Al-Yamani, O., Grant, C.A, Green, N.H., Rimmer, S. Poly(n-butyl Methacrylate) with Primary Amine End Groups for Supporting Cell Adhesion and Proliferation of Renal Epithelial Cells, International Journal of Polymeric Materials and Polymeric Biomaterials, 66(15):762-767, 2017
  4. Narice, B.F., Green, N.H., MacNeil, S., Anumba, D. Second Harmonic Generation microscopy reveals collagen fibres are more organised in the cervix of postmenopausal women, Reprod Biol Endocrinol, 14:70, 2016
  5. Developing Repair Materials for Stress Urinary Incontinence to Withstand Dynamic Distension Hillary CJ, Román S, Bullock AJ, Green NH, Chapple CR and MacNeil S. PLoS ONE 11(3):e0149971(2016)
  6. Creating a model of diseased artery damage and failure from healthy porcine aorta Noble C, Smulders N, Green NH, Lewis R, Carré MJ, Franklin SE, MacNeil S and Taylor ZA. J. Mec. Behav. Biomed. Mat. 60, 378 (2016)
  7. Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering Owen R, Sherborne C, Paterson T, Green NH, Reilly GC and Claeyssens F. J. Mec. Behav. Biomed. Mat. 54, 159 (2016)
  8. Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue engineered melanoma models Yorucu C, Lau K, Mittar S, Green NH, Raxa A, Rehman IU and MacNeil S. Appl. Spectrosc. Rev. 51, 263 (2016)
  9. Thermoresponsive, stretchable, biodegradable and biocompatible poly(glycerol sebacate)-bsed polyurethane hydrogels Frydrych M, Román S, Green NH, MacNeil S and Chen B. Polym. Chem. 6, 7974 (2015)
  10. Production and characterization of a 3D tissue-engineered human oesophageal mucosal model Green NH, Corfe BM, Bury JP and MacNeil S. JoVE 99 (2015) doi:10.3791/52693
  11. Characterisation of the physical composition (cells, carbohydrates and proteins) and community structure (bacterial, archaeal and fungal) of biofilms within Drinking Water Distribution Systems Fish KE, Collins R, Green NH, Sharpe RL, Douterelo I, Osborn AM and Boxall JB. PLoS ONE 10(2):e0115824 (2015)
  12. Rocking media over ex vivo corneas improves this model and allows the study of proinflammatory cytokines on wound healing Deshpande P, Ortega I, Sefat F, Sangwanb VS, Green NH, Claeyssens F and MacNeil S IOVS 56, 1553 (2015)
  13. Pulsatile exposure to simulated reflux leads to changes in gene expression in a 3D model of oesophageal mucosa Green NH, Nicholls Z, Heath PR, Cooper-Knock J, Corfe BM, MacNeil S and Bury JP. Int. J. Exp. Pathol., 95, 216 (2014)
  14. Dinuclear ruthenium(II) complexes as two-photon, time-resolved emission microscopy probes for cellular DNA Baggaley E, Gill MR, Green NH, Turton D, Sazanovich IV, Botchway SW, Smythe C, Haycock JW, Weinstein JA and Thomas JA. Angew. Chem. Int. Edit., 53, 3367 (2014)
  15. Monitoring fibrous scaffold guidance of three-dimensional collagen organisation using minimally-invasive second harmonic generation Delaine-Smith R, Green NH, Matcher SJ, MacNeil A and Reilly GC. PLoS ONE, 9, e89761 (2014)
  16. NF-κB is activated in oesophageal fibroblasts in response to a paracrine signal generated by acid-exposed primary oesophageal squamous cells Green N, Huang Q, Corfe B, Bury J and MacNeil S. Int. J. Exp. Pathol., 92, 345 (2011)
  17. The development and characterisation of an organotypic tissue engineered human oesophageal mucosal model Green N, Huang Q, Khan L, Battaglia G, Corfe B, MacNeil S and Bury J. Tissue Eng. Part A., 16, 1053 (2010)
  18. A new anti-cancer glycolipid monoclonal antibody, SC104, which directly induces tumour cell apoptosis Durrant LG, Harding SJ, Green NH, Buckberry LD and Parsons T. Cancer Res., 66, 11 (2006)
  19. Single molecule investigations of RNA dissociation Green NH, Williams PM, Wahab O, Davies MC, Roberts CJ, Tendler SJB and Allen S. J., 86, 3811 (2004)
  20. Force sensing and mapping by atomic force microscopy Green NH, Allen S, Davies MC, Roberts CJ, Tendler SJB and Williams PM. , 21, 65 (2002)
  21. Constraints on the transport and glycosylation of recombinant IFN-γ in Chinese hamster ovary cells and insect cells Hooker AD, Green NH, Baines AJ, Bull AT, Jenkins N, Strange PG and James DC. Bioeng., 63, 559 (1999)
  22. Efficient human IFN-γ expression in the mammary gland of transgenic mice Lagutin OV, Dobrovolsky VN, Vinogradova TV, Kyndiakov BN, Khodarovich YM, Jenkins N, James DC, Markham N and Larionov OA. J J. Interf. Cytok. Res., 19, 137 (1999)
  23. N-Glycans of recombinant human IFN-γ change during batch culture of Chinese hamster ovary cells Hooker AD, Goldman MH, Markham NH, James DC, Ison AP, Bull AT, Strange PG, Salmon I, Baines AJ, and Jenkins N. Biotechnol. Bioeng, 48, 639 (1995)