bulloughpProfessor Per Bullough

Professor of Structural Biology

Room: E36e
Tel: 0114 222 4245
Email: p.bullough@sheffield.ac.uk

Overview

Research Precis

Bacillus Spore assemblyOur laboratory focuses on two challenging areas of structural biology - membrane proteins and very large macromolecular complexes. Our main technique is high resolution cryoelectron microscopy, which we often use to complement other structural techniques such as X-ray crystallography.

Current projects fall under the following main headings:

  • Structure and assembly of large protein complexes at two dimensional interfaces- bacterial spore assembly, bacterial cell surfaces, photosynthetic proteins, bacterial toxins.
  • Energy transduction in membrane systems - photosynthesis, transporters.
  • Electron microscopy of macromolecular assemblies.

Research Keywords

Structural biology, electron microscopy, cryoEM, X-ray crystallography, bacteriology

Career History

  • 2001 - present: Professor, Dept. of Molecular Biology and Biotechnology, University of Sheffield
  • 2002 - 2005: BBSRC Research Development Fellow
  • 1995 - 2001: Lecturer, Dept. of Molecular Biology and Biotechnology, University of Sheffield
  • 1994 - 1995: Staff Scientist, MRC Laboratory of Molecular Biology, Cambridge
  • 1990 - 1994: Research Associate, Department of Biochemistry and Molecular Biology, Harvard University, USA
  • 1989 - 1990: Research Associate, Howard Hughes Medical Institute, Harvard University, USA
  • 1988 - 1989: Staff Scientist, MRC Laboratory of Molecular Biology, Cambridge
  • 1985 - 1988: PhD, MRC Laboratory of Molecular Biology and Darwin College, University of Cambridge
Research

fig1Our laboratory focuses on two challenging areas of structural biology - membrane proteins and very large macromolecular complexes. Our main technique is high resolution cryoelectron microscopy, which we often use to complement other structural techniques such as X-ray crystallography.

Current projects fall under the following main headings:

  • Structure and assembly of large protein complexes at two dimensional interfaces- bacterial spore assembly, bacterial cell surfaces, photosynthetic proteins, bacterial toxins.
  • Energy transduction in membrane systems - photosynthesis, transporters.
  • Electron microscopy of macromolecular assemblies.
Joining the lab

I welcome applications from prospective home / EU / overseas PhD students and post-doctoral fellows.
Contact me at p.bullough@sheffield.ac.uk for further information.

Journal articles

Conference proceedings papers

  • Baboolal TG, Conroy MJ, Gill K, Bullough P & Lakey JH (2005) The colicin N- OmpF translocation complex. BIOPHYSICAL JOURNAL, Vol. 88(1) (pp 50A-51A)
  • Bullough PA, Jamieson SJ, Walz T, Hunter CN, Bowers CM, Karrasch S, Ghosh R, Henderson R & Subramaniam S (1999) Energy production in biological systems probed by electron crystallography. ELECTRON MICROSCOPY AND ANALYSIS 1999(161) (pp 127-132)
  • Bullough PA (1996) Conformational changes in influenza haemagglutinin at the pH of membrane fusion. OPTIONS FOR THE CONTROL OF INFLUENZA III, Vol. 1123 (pp 307-314)
  • Karrasch S, Bullough PA & Ghosh R (1995) 8.5 angstrom projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits. PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL I (pp 81-84)
  • SKEHEL JJ, STEINHAUER D, WHARTON SA, BULLOUGH PA, HUGHSON FM, WATOWICH SJ & WILEY DC (1994) RECEPTOR BINDING AND MEMBRANE FUSION BY INFLUENZA HEMAGGLUTININ. CELLULAR RECEPTORS FOR ANIMAL VIRUSES (pp 187-193)