Research |
Research PrecisCellular morphogeneisis in the human pathogen Candida albicans
The focus of our current research is to understand how hyphal growth is controlled by the action of protein kinases. We have identified the particular kinases and the proteins they target. We have investigated the physiological role of these phosphorylation events by mutating the phosphoacceptor sites in the target proteins to residues that either mimic phosphorylation, so the proteins behave as if they were permanently phosphorylated, or to non-phosphorylatable residues to investigate the consequences of the absence of phosphorylation. We are now employing Mass Spectroscopy-based methods to carry out unbiased, proteome-wide surveys of the targets of kinases known to be essential for hyphal growth.
Research KeywordsMicrobiology, fungal pathogenesis, Candida albicans, hyphal morphogenesis, polarised growth, cell biology, phosphorylation, kinases |
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Teaching |
Level 3 ModulesMBB320 Human Genetics 1 (Module Coordinator) Level 2 ModulesMBB265 Practical Molecular Bioscience 2 Level 1 ModulesMBB162 Genetics |
Career History |
Career History
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Publications
Books
- Sudbery P (2009) Human Molecular Genetics. Benjamin-Cummings Pub Co.
- Sudbery P (2002) Human Molecular Genetics. Prentice Hall.
- Sudbery P (1998) Human Molecular Genetics. Prentice Hall.
Journal articles
- Kaneva IN, Sudbery IM, Dickman MJ & Sudbery PE (2019) Proteins that physically interact with the phosphatase Cdc14 in Candida albicans have diverse roles in the cell cycle. Scientific Reports, 9. View this article in WRRO
- Kaneva IN, Longworth J, Sudbery PE & Dickman MJ (2018) Quantitative Proteomic Analysis in Candida albicans
Using SILAC-Based Mass Spectrometry. PROTEOMICS, 18(5-6), 1700278-1700278. View this article in WRRO
- Greig JA, Sudbery IM, Richardson JP, Naglik JR, Wang Y & Sudbery PE (2015) Cell Cycle-Independent Phospho-Regulation of Fkh2 during Hyphal Growth Regulates Candida albicans Pathogenesis. PLOS Pathogens, 11(1), e1004630-e1004630. View this article in WRRO
- Caballero-Lima D, Hautbergue GM, Wilson SA & Sudbery PE (2014) In C andida albicans hyphae, Sec2p is physically associated with SEC2 mRNA on secretory vesicles. Molecular Microbiology, 94(4), 828-842. View this article in WRRO
- Caballero-Lima D & Sudbery PE (2014) In Candida albicans, phosphorylation of Exo84 by Cdk1-Hgc1 is necessary for efficient hyphal extension.. Mol Biol Cell, 25(7), 1097-1110. View this article in WRRO
- Caballero-Lima D, Kaneva IN, Watton SP, Sudbery PE & Craven CJ (2013) The spatial distribution of the exocyst and actin cortical patches is sufficient to organize hyphal tip growth.. Eukaryot Cell, 12(7), 998-1008. View this article in WRRO
- Alaalm L, Bermejo G, Yin Z, Argimon S, Walker J, Aldana C, Brown A, Correa-Bordes J & Sudbery PE (2012) In Candida albicans the transcriptional regulator Nrg1 is regulated at multiple post-transcriptional levels by kinase action. MYCOSES, 55, 12-12.
- Sudbery PE (2011) Growth of Candida albicans hyphae.. Nat Rev Microbiol, 9(10), 737-748.
- Kim J & Sudbery P (2011) Candida albicans, a major human fungal pathogen.. J Microbiol, 49(2), 171-177.
- Sudbery P (2011) Fluorescent proteins illuminate the structure and function of the hyphal tip apparatus. Fungal Genetics and Biology, 48(9), 849-857.
- Chapa-y-Lazo B, Lee S, Regan H & Sudbery P (2011) The mating projections of Saccharomyces cerevisiae and Candida albicans show key characteristics of hyphal growth. Fungal Biology.
- Jones LA & Sudbery PE (2010) Spitzenkorper, exocyst, and polarisome components in Candida albicans hyphae show different patterns of localization and have distinct dynamic properties.. Eukaryot Cell, 9(10), 1455-1465.
- Bishop A, Lane R, Beniston R, Chapa-y-Lazo B, Smythe C & Sudbery P (2010) Hyphal growth in Candida albicans requires the phosphorylation of Sec2 by the Cdc28-Ccn1/Hgc1 kinase.. EMBO J, 29(17), 2930-2942.
- Grubb SEW, Murdoch C, Sudbery PE, Saville SP, Lopez-Ribot JL & Thornhill MH (2009) Adhesion of Candida albicans to endothelial cells under physiological conditions of flow.. Infect Immun, 77(9), 3872-3878.
- Butler G, Rasmussen MD, Lin MF, Santos MAS, Sakthikumar S, Munro CA, Rheinbay E, Grabherr M, Forche A, Reedy JL , Agrafioti I et al (2009) Evolution of pathogenicity and sexual reproduction in eight Candida genomes.. Nature, 459(7247), 657-662.
- Leadsham JE, Miller K, Ayscough KR, Colombo S, Martegani E, Sudbery P & Gourlay CW (2009) Whi2p links nutritional sensing to actin-dependent Ras-cAMP-PKA regulation and apoptosis in yeast.. J Cell Sci, 122(Pt 5), 706-715.
- Gladfelter AS & Sudbery P (2008) Septins in Four Model Fungal Systems: Diversity in Form and Function, 125-146.
- Grubb SEW, Murdoch C, Sudbery PE, Saville SP, Lopez-Ribot JL & Thornhill MH (2008) Candida albicans-endothelial cell interactions: a key step in the pathogenesis of systemic candidiasis.. Infect Immun, 76(10), 4370-4377.
- Sudbery PE (2008) Regulation of polarised growth in fungi. Fungal Biology Reviews, 22(2), 44-55. View this article in WRRO
- Sudbery PE & Gladfelter AS (2008) Pathocycles.. Fungal Genet Biol, 45(1), 1-5.
- Sudbery P (2007) Morphogenesis of a human fungal pathogen requires septin phosphorylation.. Dev Cell, 13(3), 315-316.
- Court H & Sudbery P (2007) Regulation of Cdc42 GTPase activity in the formation of hyphae in Candida albicans.. Mol Biol Cell, 18(1), 265-281.
- Crampin H, Finley K, Gerami-Nejad M, Court H, Gale C, Berman J & Sudbery P (2005) Candida albicans hyphae have a Spitzenkörper that is distinct from the polarisome found in yeast and pseudohyphae.. J Cell Sci, 118(Pt 13), 2935-2947.
- Chapa y Lazo B, Bates S & Sudbery P (2005) The G1 cyclin Cln3 regulates morphogenesis in Candida albicans.. Eukaryot Cell, 4(1), 90-94.
- Sudbery P, Gow N & Berman J (2004) The distinct morphogenic states of Candida albicans.. Trends Microbiol, 12(7), 317-324.
- Ceccato-Antonini SR & Sudbery PE (2004) Filamentous growth in Saccharomyces cerevisiae. BRAZ J MICROBIOL, 35(3), 173-181.
- Wightman R, Bates S, Amornrrattanapan P & Sudbery P (2004) In Candida albicans, the Nim1 kinases Gin4 and Hsl1 negatively regulate pseudohypha formation and Gin4 also controls septin organization.. J Cell Biol, 164(4), 581-591.
- Care A, Vousden KA, Binley KM, Radcliffe P, Trevethick J, Mannazzu I & Sudbery PE (2004) A synthetic lethal screen identifies a role for the cortical actin patch/endocytosis complex in the response to nutrient deprivation in Saccharomyces cerevisiae.. Genetics, 166(2), 707-719.
- Sudbery P (2003) Hansenula polymorpha: Biology and Applications. Edited by G. Gellissen. Wiley-VCH, Weinham, 2002. Yeast, 20(15), 1307-1308.
- Martinez-Anaya C, Dickinson JR & Sudbery PE (2003) In yeast, the pseudohyphal phenotype induced by isoamyl alcohol results from the operation of the morphogenesis checkpoint.. J Cell Sci, 116(Pt 16), 3423-3431.
- Berman J & Sudbery PE (2002) Candida Albicans: a molecular revolution built on lessons from budding yeast.. Nat Rev Genet, 3(12), 918-930.
- Sudbery P (2002) Cell biology. When wee meets whi.. Science, 297(5580), 351-352.
- West A, Duffy K, Binley K, Radcliffe P & Sudbery P (2001) SIW14, a putative tyrosine phosphatase involved in cell integrity in Saccharomyces cerevisiae.. YEAST, 18, S206-S206.
- Sudbery PE (2001) The germ tubes of Candida albicans hyphae and pseudohyphae show different patterns of septin ring localization.. Mol Microbiol, 41(1), 19-31.
- Cox H, Mead D, Sudbery P, Eland RM, Mannazzu I & Evans L (2000) Constitutive expression of recombinant proteins in the methylotrophic yeast Hansenula polymorpha using the PMA1 promoter.. Yeast, 16(13), 1191-1203.
- Leng P, Sudbery PE & Brown AJ (2000) Rad6p represses yeast-hypha morphogenesis in the human fungal pathogen Candida albicans.. Mol Microbiol, 35(5), 1264-1275.
- Care RS, Trevethick J, Binley KM & Sudbery PE (1999) The MET3 promoter: a new tool for Candida albicans molecular genetics.. Mol Microbiol, 34(4), 792-798.
- Binley KM, Radcliffe PA, Trevethick J, Duffy KA & Sudbery PE (1999) The yeast PRS3 gene is required for cell integrity, cell cycle arrest upon nutrient deprivation, ion homeostasis and the proper organization of the actin cytoskeleton.. Yeast, 15(14), 1459-1469.
- Radcliffe P, Trevethick J, Tyers M & Sudbery P (1997) Deregulation of CLN1 and CLN2 in the Saccharomyces cerevisiae whi2 mutant.. Yeast, 13(8), 707-715.
- Radcliffe PA, Binley KM, Trevethick J, Hall M & Sudbery PE (1997) Filamentous growth of the budding yeast Saccharomyces cerevisiae induced by overexpression of the WHi2 gene.. Microbiology, 143 ( Pt 6), 1867-1876.
- Sudbery PE (1996) The expression of recombinant proteins in yeasts.. Curr Opin Biotechnol, 7(5), 517-524.
- Yavuz MO, Ashton SM, Deakin ED, Spencer ME & Sudbery PE (1996) Expression of the major bean proteins from Theobroma cacao (cocoa) in the yeasts Hansenula polymorpha and Saccharomyces cerevisiae.. J Biotechnol, 46(1), 43-54.
- MANNAZZU I, SUDBERY PE, BERARDI E & FATICHENTI F (1995) PROMOTER ISOLATION IN HANSENULA-POLYMORPHA. ANN MICROBIOL ENZIM, 45, 209-218.
- Sudbery PE (1994) The non-Saccharomyces yeasts.. Yeast, 10(13), 1707-1726.
- Wedlock N, Furmaniak J, Fowler S, Kiso Y, Bednarek J, Baumann-Antczak A, Morteo C, Sudbery P, Hinchcliff A & Rees Smith B (1993) Expression of human thyroid peroxidase in the yeasts Saccharomyces cerevisiae and Hansenula polymorpha.. J Mol Endocrinol, 10(3), 325-336.
- Hodgkins M, Mead D, Ballance DJ, Goodey A & Sudbery P (1993) Expression of the glucose oxidase gene from Aspergillus niger in Hansenula polymorpha and its use as a reporter gene to isolate regulatory mutations.. Yeast, 9(6), 625-635.
- Veale RA, Giuseppin ML, van Eijk HM, Sudbery PE & Verrips CT (1992) Development of a strain of Hansenula polymorpha for the efficient expression of guar alpha-galactosidase.. Yeast, 8(5), 361-372.
- Veale RA, Giuseppin MLF, Van Eijk HMJ, Sudbery PE & Verrips CT (1992) Development of a strain of hansenula polymorpha for the efficient expression of guar α‐galactosidase. Yeast, 8(9), 813-813.
- Fellinger AJ, Verbakel JM, Veale RA, Sudbery PE, Bom IJ, Overbeeke N & Verrips CT (1991) Expression of the alpha-galactosidase from Cyamopsis tetragonoloba (guar) by Hansenula polymorpha.. Yeast, 7(5), 463-473.
- Sudbery P (1991) Gene cloning, 2nd edn by T. A. Brown, Chapman & Hall, 1990. £12.95 (xii + 286 pages) ISBN 0 412 34210 3. Trends in Biotechnology, 9(1), 212-213.
- Mountain HA & Sudbery PE (1990) The relationship of growth rate and catabolite repression with WHI2 expression and cell size in Saccharomyces cerevisiae.. J Gen Microbiol, 136(4), 733-737.
- Mountain HA & Sudbery PE (1990) Regulation of the Saccharomyces cerevisiae WHI2 gene.. J Gen Microbiol, 136(4), 727-732.
- GLEESON MA & SUDBERY PE (1988) GENETIC-ANALYSIS IN THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA. YEAST, 4(4), 293-303.
- Sudbery PE, Gleeson MA, Veale RA, Ledeboer AM & Zoetmulder MC (1988) Hansenula polymorpha as a novel yeast system for the expression of heterologous genes.. Biochem Soc Trans, 16(6), 1081-1083.
- Rahman DR, Sudbery PE, Kelly S & Marison IW (1988) The effect of dissolved oxygen concentration on the growth physiology of Saccharomyces cerevisiae whi2 mutants.. J Gen Microbiol, 134(8), 2241-2248.
- Kelly DE, Trevethick J, Mountain H & Sudbery PE (1988) Transcript characterisation, gene disruption and nucleotide sequence of the Saccharomyces cerevisiae WH12 gene.. Gene, 66(2), 205-213.
- GLEESON MA & SUDBERY PE (1988) THE METHYLOTROPHIC YEASTS. YEAST, 4(1), 1-15.
- Rahman DR, Sudbery PE, Kelly S & Marison IW (1988) The effect of dissolved oxygen concentration on the growth physiology of Saccharomyces cerevisiae whi2 mutants.. Journal of general microbiology, 134(8), 2241-2248.
- GLEESON MA, ORTORI GS & SUDBERY PE (1986) TRANSFORMATION OF THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA. J GEN MICROBIOL, 132, 3459-3465.
- SAUL DJ, Walton EF, SUDBERY PE & CARTER BLA (1985) Saccharomyces cerevisiae whi2 Mutants in Stationary Phase Retain the Properties of Exponentially Growing Cells. Microbiology, 131(9), 2245-2251.
- Saul DJ & Sudbery PE (1985) Molecular cloning of WHI2, a gene involved in the regulation of cell proliferation in Saccharomyces cerevisiae.. J Gen Microbiol, 131(7), 1797-1806.
- SAUL DJ, WALTON EF, SUDBERY PE & CARTER BLA (1985) SACCHAROMYCES-CEREVISIAE WHI2 MUTANTS IN STATIONARY PHASE RETAIN THE PROPERTIES OF EXPONENTIALLY GROWING-CELLS. J GEN MICROBIOL, 131(SEP), 2245-2251.
- CARRAU JL, DEAZEVEDO JL, SUDBERY P & CAMPBELL D (1982) METHODS FOR RECOVERING FUSION PRODUCTS AMONG ENOLOGICAL STRAINS OF SACCHAROMYCES-CEREVISIAE AND SCHIZOSACCHAROMYCES-POMBE. REV BRAS GENET, 5(1), 221-226.
- Sudbery PE, Goodey AR & Carter BL (1980) Genes which control cell proliferation in the yeast Saccharomyces cerevisiae.. Nature, 288(5789), 401-404.
- Carter BL & Sudbery PE (1980) Small-sized mutants of Saccharomyces cerevisiae.. Genetics, 96(3), 561-566.
- SUDBERY P, HAUGLI K & HAUGLI F (1978) ENRICHMENT AND SCREENING OF HEAT-SENSITIVE MUTANTS OF PHYSARUM-POLYCEPHALUM. GENET RES, 31(1), 1-12.
- Sudbery PE & Grant WD (1976) The control of mitosis in Physarum polycephalum: the effect of delaying mitosis and evidence for the operation of the control mechanism in the absence of growth.. J Cell Sci, 22(1), 59-65.
- Sudbery PE & Grant WD (1975) The control of mitosis in Physarum polycephalum. The effect of lowering the DNA: mass ratio by UV irradiation.. Exp Cell Res, 95(2), 405-415.
- Fantes PA, Grant WD, Pritchard RH, Sudbery PE & Wheals AE (1975) The regulation of cell size and the control of mitosis.. J Theor Biol, 50(1), 213-244.
- MCCULLOU.CH , COOKE DJ, FOXON JL, SUDBERY PE & GRANT WD (1973) NUCLEAR DNA CONTENT AND SENESCENCE IN PHYSARUM-POLYCEPHALUM. NATURE-NEW BIOL, 245(148), 263-265.
Chapters
- Haugli FB, Cooke D & Sudbery P (1980) V. The Genetic Approach in the Analysis of the Biology of Physarum polycephalum, Growth and Differentiation in Physarum Polycephalum (pp. 129-156). Princeton University Press
- Sudbery PE () Genetic Engineering of Yeast, Biotechnology Set (pp. 507-528). Wiley-VCH Verlag GmbH
- Sudbery PE () Genetic Engineering of Yeast, Biotechnology (pp. 507-528). Wiley-VCH Verlag GmbH
- Sudbery P & Court H () Polarised Growth in Fungi, Biology of the Fungal Cell (pp. 137-166). Springer Berlin Heidelberg