Dr Rebecca Corrigan
Sir Henry Dale Research Fellow
0114 222 4238
Honours and Distinctions
Microbiology, S. aureus, nucleotide signalling, (p)ppGpp signalling
My research involves an in-depth characterisation of nucleotide signalling systems in the Gram-positive pathogen Staphylococcus aureus. S. aureus is a human pathogen responsible for a vast array of disease and morbidity worldwide, a problem that is exacerbated by the spread of antibiotic-resistant strains such as methicillin resistant S. aureus (MRSA). When this bacterium invades a human host it encounters a number of different stresses, such as nutrient limitation. The bacteria respond to these stresses by switching on a nucleotide signalling system called the stringent response. This response results in the synthesis of two small nucleotides, collectively referred to as (p)ppGpp, which can be made in the cell by three different enzymes – RSH, RelP and RelQ. These nucleotides are the effectors of the stringent response and function by binding to target proteins leading to the bacterial cells shutting down active growth and entering a persistent state that promotes survival.
Figure 1: Synthesis and degradation of (p)ppGpp in S. aureus. In the Firmicutes the RelA/SpoT homologue RSH is responsible for (p)ppGpp synthesis (syn) and hydrolysis (HD) in response to amino acid deprivation. The genome also encodes for two additional monofunctional synthases, RelP and RelQ.
My previous research has led to the development of a genome-wide approach to analyse nucleotide-protein interactions. The current focus of the lab is on utilising this methodology, in conjunction with biochemical assays, to identify binding targets for (p)ppGpp in S. aureus in order to precisely map how these nucleotides function in a bacterial cell.
Figure 2: Flowchart outlining the steps involved in a genome-wide nucleotide-protein interaction screen.
By mapping of the (p)ppGpp signalling network this research will provide key insights into the functioning of (p)ppGpp and so generate important mechanistic data on the pathogenesis of S. aureus.
Level 3 Modules
|Joining the Lab||
If you are interested in joining my group you can contact me directly by email at firstname.lastname@example.org
Funded PhD studentships will be advertised here as they become available but well qualified graduates, including those intending to self fund, should contact me directly to discuss possible projects.
Postdocs who wish to apply for a fellowship to join the group are more than welcome. Please contact me directly to discuss potential projects.
Project Students and Internships:
Students wishing to apply for summer internships are welcome and should send a CV describing their motivations and interests.
- Schuster CF, Bellows LE, Tosi T, Campeotto I, Corrigan RM, Freemont P & Grundling A (2016) The second messenger c-di-AMP inhibits the osmolyte uptake system OpuC in Staphylococcus aureus. Science Signaling, 9(441). View this article in WRRO
- Corrigan R, Bellows LE, Wood A & Grundling A (2016) ppGpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in Gram-positive bacteria. Proceedings of the National Academy of Sciences of the United States of America, 113(12), E1710-E1719. View this article in WRRO
- Corrigan RM, Bowman L, Willis AR, Kaever V & Gründling A (2015) Cross-talk between Two Nucleotide-signaling Pathways in Staphylococcus aureus. Journal of Biological Chemistry, 290(9), 5826-5839. View this article in WRRO
- Reichmann NT, Piçarra Cassona C, Monteiro JM, Bottomley AL, Corrigan RM, Foster SJ, Pinho MG & Gründling A (2014) Differential localization of LTA synthesis proteins and their interaction with the cell division machinery in Staphylococcus aureus. Molecular Microbiology, 92(2), 273-286. View this article in WRRO
- Reichmann NT, Cassona CP, Monteiro JM, Bottomley AL, Corrigan RM, Foster SJ, Pinho MG & Gruendling A (2014) Differential localization of LTA synthesis proteins and their interaction with the cell division machinery in Staphylococcus aureus. NEW ZEALAND JOURNAL OF ZOOLOGY, 41(1), 273-286.
- Corrigan RM & Gründling A (2013) Cyclic di-AMP: another second messenger enters the fray. Nature Reviews Microbiology, 11(8), 513-524.
- Corrigan RM, Campeotto I, Jeganathan T, Roelofs KG, Lee VT & Grundling A (2013) Systematic identification of conserved bacterial c-di-AMP receptor proteins. Proceedings of the National Academy of Sciences, 110(22), 9084-9089. View this article in WRRO
- Smith EJ, Corrigan RM, van der Sluis T, Gründling A, Speziale P, Geoghegan JA & Foster TJ (2012) The immune evasion protein Sbi of Staphylococcus aureus occurs both extracellularly and anchored to the cell envelope by binding lipoteichoic acid. Molecular Microbiology, 83(4), 789-804. View this article in WRRO
- Corrigan RM, Abbott JC, Burhenne H, Kaever V & Gründling A (2011) c-di-AMP Is a New Second Messenger in Staphylococcus aureus with a Role in Controlling Cell Size and Envelope Stress. PLoS Pathogens, 7(9), e1002217-e1002217. View this article in WRRO
- Xia G, Corrigan RM, Winstel V, Goerke C, Grundling A & Peschel A (2011) Wall Teichoic Acid-Dependent Adsorption of Staphylococcal Siphovirus and Myovirus. Journal of Bacteriology, 193(15), 4006-4009.
- Wörmann ME, Corrigan RM, Simpson PJ, Matthews SJ & Gründling A (2011) Enzymatic activities and functional interdependencies of Bacillus subtilis lipoteichoic acid synthesis enzymes. Molecular Microbiology, 79(3), 566-583. View this article in WRRO
- Geoghegan JA, Corrigan RM, Gruszka DT, Speziale P, O'Gara JP, Potts JR & Foster TJ (2010) Role of Surface Protein SasG in Biofilm Formation by Staphylococcus aureus. Journal of Bacteriology, 192(21), 5663-5673.
- Corrigan RM & Foster TJ (2009) An improved tetracycline-inducible expression vector for Staphylococcus aureus. Plasmid, 61(2), 126-129.
- Corrigan RM, Miajlovic H & Foster TJ (2009) Surface proteins that promote adherence of Staphylococcus aureus to human desquamated nasal epithelial cells. BMC Microbiology, 9(1), 22-22. View this article in WRRO
- Traber KE, Lee E, Benson S, Corrigan R, Cantera M, Shopsin B & Novick RP (2008) agr function in clinical Staphylococcus aureus isolates. Microbiology, 154(8), 2265-2274.
- Corrigan RM, Rigby D, Handley P & Foster TJ (2007) The role of Staphylococcus aureus surface protein SasG in adherence and biofilm formation. Microbiology, 153(8), 2435-2446.
- Wright JS, Traber KE, Corrigan R, Benson SA, Musser JM & Novick RP (2005) The agr Radiation: an Early Event in the Evolution of Staphylococci. Journal of Bacteriology, 187(16), 5585-5594.