Dr Rachid Tazi-Ahnini

Department of Infection, Immunity and Cardiovascular Disease

Lecturer in Dermatology

r.taziahnini@sheffield.ac.uk
+44 114 215 9556

Full contact details

Dr Rachid Tazi-Ahnini
Department of Infection, Immunity and Cardiovascular Disease
The Medical School
Beech Hill Road
Sheffield
S10 2RX
Profile

I studied for my Bachelor´s and Master´s degrees in molecular and cellular biology at the University Paul Sabatier, Toulouse III-France.  In 1994 I began PhD studies in human genetics at the laboratories of "Centre National de Recherche Scientifique", Toulouse. 

As part of my PhD, I worked on positional cloning of multigenic family butyrophilin, located in the distal part of the major histocompatibility complex (MHC).  I moved to Britain for a postdoctoral appointment in 1997at the University of Sheffield. Because of my background in MHC structure, evolution and genetics, I subsequently focussed most of my work on the genetics of the MHC in psoriasis and alopecia areata.

In January 2000, I was appointed by the University of Sheffield to the post of Lecturer in Molecular Dermatology to develop genetic studies of inflammatory skin diseases including alopecia areata, psoriasis, atopic dermatitis and vitiligo.

 Recently, I become very interested in understanding the mechanism underlining the pathogenesis of some autoimmune diseases such as alopecia areata, vitiligo and thyroid diseases.  I am particularly interested in identifying auto-antigens involved in the pathogenesis of these diseases.

Research interests

My research interests are determination of the function of the autoimmune regulator (AIRE) gene and its role in the pathogenesis of some autoimmune diseases such as alopecia areata, vitiligo and thyroid diseases.

In our department, we are using Autoimmune Polyglandular Syndrome type I (APS-1) as a model to study the pathogenesis of these disorders.  APS-1 is a rare disorder characterised by an unusual pathogenesis within the range of autoimmune disorders, most of which are more typically polygenic traits such as hypoparathyroidism and Addison´s disease, diabetes, alopecia areata and vitiligo.  APS-1 is an autosomal recessive disorder caused by loss-of-function mutations of the Autoimmune Regulator (AIRE) gene located at chromosome 21q22.3.

I am also interested in the genetics of some skin diseases such as psoriasis, atopic dermatitis, alopecia areata and vitiligo.  We use a genome-wide association study to identify genetic loci of these diseases.  Candidate genes within susceptibility loci are screened for single nucleotide polymorphisms (SNPs) and the distribution of each SNP is then compared between cases and controls.  We also used transmissions disequilibrium tests (TDT) to test for transmission distortion of SNPs or haplotypes using trios (patient and parents).

Current projects

  • AIRE encodes for a transcription factor that has been found to regulate the promiscuous expression of tissue restricted antigens in the thymus.  This function increases the repertoire of antigens presented in the thymus for negative selection of immature thymocytes in the central tolerance process.  We are investigating the effects of polymorphisms in the AIRE promoter on AIRE expression as well as the mechanism of AIRE regulated gene expression.  We are using site-specific recombination techniques to generate a variety of different human cell line models with which to investigate the function and regulation of AIRE.
  • The role of the autoimmune regulator (AIRE) gene is fundamental to alopecia areata (AA) arising in the autoimmune polyendocrine syndrome type 1 (APS-1) and we are currently analysing the role of AIRE in sporadic AA cases using a cellular model of thymic cell gene expression.  Demonstration that autoantigens identified in AA cases non-associated with APS-1 are also regulated by the AIRE gene would strongly support the role of AIRE in the pathogenesis of AA.
    Refinement of the identity and role of autoantigens in this complex polygenic disorder also has the potential to provide new insights into the pathogenesis of a spectrum of organ-specific autoimmune diseases which occur more commonly in patients and families with AA such as vitiligo and autoimmune thyroid disease.
Publications

Show: Featured publications All publications

Journal articles

All publications

Journal articles

Chapters

  • Cork MJ, Moustafa M, Danby S, Vasilopoulos Y, Tazi-Ahnini R, Ward SJ, Hadgraft J, Lane ME, Guy R & MacGowan A (2009) Skin Barrier Dysfunction in Atopic Dermatitis, Basic and Clinical Dermatology (pp. 211-240). Informa Healthcare RIS download Bibtex download
  • Cork MJ, Danby SG, Vasilopoulos Y, Hadgraft J, Lane M, Moustafa M, Guy R, MacGowan A, Tazi-Ahnini R & Ward SJ (2009) Epidermal Barrier Dysfunction in Atopic Dermatitis In Rawlings L (Ed.), Skin Moisturization USA: Informa Healthcare USA Inc.. RIS download Bibtex download
  • McDonagh AJG, Tazi-Ahnini R & Messenger AG (2003) Alopecia areata: An update on etiology and pathogenesis, Skin, Hair, and Nails: Structure and Function (pp. 359-375). RIS download Bibtex download

Conference proceedings papers

  • Lovewell TRJ, McDonagh AJ & Tazi-Ahnini R (2010) Use of a novel integrated reporter system to measure the effect of polymorphisms on AIRE gene promoter activity. BRITISH JOURNAL OF DERMATOLOGY, Vol. 162(4) (pp 941-941) RIS download Bibtex download
  • Lovewell TRJ, McDonagh AJG & Tazi-Ahnini R (2008) Single nucleotide polymorphisms in the AIRE promoter can affect its transcriptional efficiency. BRITISH JOURNAL OF DERMATOLOGY, Vol. 158(4) (pp 894-895) RIS download Bibtex download
  • Kemp EH, McDonagh AJG, Wengraf DA, Messenger AG, Gawkodger DJ, Cork MJ & Tazi-Ahnini R (2007) Severe alopecia areata is associated with a functional polymorphism in the PTPN22 gene. IMMUNOLOGY, Vol. 120 (pp 70-70) RIS download Bibtex download
  • Simon M, Tazi-Ahnini R, Jonca N, Cork MJ & Serre G (2006) Reduced and abnormal proteolysis of corneodesmosomal proteins in psoriatic skin. JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol. 126 (pp 116-116) RIS download Bibtex download
  • Vasilopoulos Y, Cork MJ, Sagoo GS, Ward SJ, Duff GW & Tazi-Ahnini R (2006) A synonymous single nucleotide polymorphism in human cystatin A, a candidate gene in the PSORS5 locus, confers susceptibility to psoriasis. BRITISH JOURNAL OF DERMATOLOGY, Vol. 155(1) (pp 235-235) RIS download Bibtex download
  • Lovewell TRJ, Cork MJ, Wengraf DA, Messenger AG, McDonagh AJG & Tazi-Ahnini R (2006) A study on the effect of genetic variants of the autoimmune regulator (AIRE) promoter, on AIRE activity and downstream Aire-regulated genes. BRITISH JOURNAL OF DERMATOLOGY, Vol. 155(1) (pp 250-250) RIS download Bibtex download
  • Cork MJ, Robinson DA, Vasilopoulos Y, Ferguson A, Moustafa M, Tazi-Ahnini R & Ward SJ (2005) Treatment of atopic dermatitis from a skin barrier perspective. JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol. 125(3) (pp 611-611) RIS download Bibtex download
  • Cork MJ, Robinson D, Vasilopoulos Y, Ferguson A, Moustafa M, Tazi-Ahnini R & Ward SJ (2005) Skin barrier damage: Cause or consequence of atopic dermatitis?. JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol. 125(3) (pp 605-605) RIS download Bibtex download
  • Cork MJ, Timmins J, Holden C, Carr J, Berry V, Tazi-Ahnini R & Ward SJ (2004) An audit of adverse drug reactions to aqueous cream in children with atopic eczema. BRITISH JOURNAL OF DERMATOLOGY, Vol. 151 (pp 57-58) RIS download Bibtex download
  • Simon M, Tazi-Ahnini R, Cork MJ & Serre G (2003) Abnormal proteolysis of corneodesmosin in psoriatic skin. JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol. 121(6) (pp 1568-1568) RIS download Bibtex download
  • Tazi-Ahnini R, Vasilopoulos Y, Henderson JK, Murphy R, Williams H, Ward SJ, Duff GW & Cork MJ (2003) Role of variants within genes regulating the skin barrier in the pathogenesis of atopic eczema. BRITISH JOURNAL OF DERMATOLOGY, Vol. 149 (pp 4-4) RIS download Bibtex download
  • Messenger AG, McDonagh AJ & Tazi-Ahnini R (2003) Genetic factors in alopecia areata. EXPERIMENTAL DERMATOLOGY, Vol. 12(2) (pp 224-224) RIS download Bibtex download
  • Wengraf DA, McDonagh AJG, Cork MJ, Gawkrodger D, Messenger AG & Tazi-Ahnini R (2003) Genetic analysis of the autoimmune regulator (aire) gene: potential role of aire in susceptibility to alopecia universalis. BRITISH JOURNAL OF DERMATOLOGY, Vol. 148(4) (pp 849-850) RIS download Bibtex download
  • McDonagh AJG, Messenger AG, Birch MP, Cork MJ & Tazi-Ahnini R (2003) Alopecia areata: age at onset, atopy & disease associations. BRITISH JOURNAL OF DERMATOLOGY, Vol. 148(4) (pp 866-867) RIS download Bibtex download

Theses / Dissertations

  • Tazi-Ahnini R (2013) Screening for protein-protein interaction at the caspase recruitment domain on the autoimmune regulator protein. RIS download Bibtex download
  • Tazi-Ahnini R (2013) A study to determine the temporal changes in expression profile of Aire-regulated genes after induction of Aire expression. RIS download Bibtex download
  • Tazi-Ahnini R (2012) A study to determine the role of the JAK-STAT pathway in AIRE-regulated gene expression. RIS download Bibtex download
  • Tazi-Ahnini R (2008) A study into the regulation and function of the autoimmune regulator (AIRE) gene. RIS download Bibtex download
  • Lovewell TRJ (2008) A study into the Regulation and Function of the Autoimmune Regulator (AIRE) Gene. University of Sheffield. RIS download Bibtex download
  • Tazi-Ahnini R (2007) The role of the autoimmune regulator (AIRE) gene in alopecia areata: genetic analysis of AIRE haplotypes. RIS download Bibtex download
  • Tazi-Ahnini R (2007) Refining the localisation of the psoriasis susceptibility locus (PSORS4) on chromosome 1q21. RIS download Bibtex download
  • Vasilopoulos Y (2007) The Role of Protease and Protease Inhibitors in the Regulation of the Skin Barrier: genetic predisposition to atopic dematitis and psoriasis. University of Sheffield. RIS download Bibtex download
  • Wegraf DA (2007) The role of the autoimmune gene (AIRE) gene in alopecia areata: genetic analysis of AIRE haplotypes. University of Sheffield. RIS download Bibtex download
  • Sagoo GS (2007) Refining the localisation of the Psoriasis susceptibility locus (PSORS4) on chromosome 1q21. University of Sheffield. RIS download Bibtex download
  • Tazi-Ahnini R (2006) The role of protease and protease inhibitors in the regulation of the skin barrier: genetic predisposition to atopic dermatitis and psoriasis. RIS download Bibtex download
  • Tazi-Ahnini R (2005) Role of cystatin A, a cystein protease inhibitor in the pathogenesis of psoriasis. RIS download Bibtex download
  • Tazi-Ahnini R (2004) The role of alpha-helix coiled coil rod homologue (HCR) in the pathogenesis of psoriasis. RIS download Bibtex download
  • Tazi-Ahnini R (2003) Characterisation of psoriasis susceptibility genes within the major histocompatibility complex-epidermal gene cluster (MHC-EGC). RIS download Bibtex download
  • Tazi-Ahnini R (2003) Genetic and functional analysis of the AIRE gene promoter: determination of its role in the pathogenesis of alopecia areata. RIS download Bibtex download
  • Tazi-Ahnini R (2001) The genetic basis of psoriasis. RIS download Bibtex download

Datasets

Research group

Team Members

  • Dr Thomas Lovewell, post-doctoral Research Assistant, 2008-present, funded by The Ministry of High Education, Saudi Arabia
  • Dr Osama Al-Amer, post-doctoral Research Assistant, funded by The Ministry of High Education, Saudi Arabia, 2014-16,
  • Dr Fatma Hamed, PhD student, 2014-16, funded by the Libyan Government he Ministry of High Education
  • Mrs Sarah Al-Maghribi. PhD student, 2014-16, funded by The Ministry of High Education, Saudi Arabia
Teaching interests
Professional activities
  • Regular reviewer for several Molecular Dermatology and Human Molecular Genetics journals.
  • Reviewer for national and international grants.
  • Member of Organising Committee of the 7th World Congress for Hair Research I Edinburgh, 4-6th May, 2013; www.hair2013.org/committee.asp