Dr Paul Heath

Department of Neuroscience

Senior Scientific Officer

p.heath@sheffield.ac.uk
+44 114 222 2254

Full contact details

Dr Paul Heath
Department of Neuroscience
385a Glossop Road
Sheffield
S10 2HQ
Profile
  • 2007 Senior Scientific Officer, Manager of Affymetrix microarray core facility, University of Sheffield, UK
  • 2001–2007 Postdoctoral Research Associate. Academic Neurology Unit, University of Sheffield.
  • 1998-2001 Associate programmer, HSBC Bank, Griffin House, Silver Street Head, Sheffield.
  • 1994-1998 Postdoctoral Research Associate, Department of Biomedical Science, University of Sheffield.
  • 1990-1994 Research Assistant, Department of Biomedical Science, University of Sheffield.
  • Jan 1990–Jun 1990 Experimental Officer, Biotechnology, I.C.I. Pharmaceuticals, Alderley Park, Macclesfield, Cheshire.
  • 1987-1989 Experimental Officer, I.C.I. Diagnostics, Gadbrook Park, Northwich, Cheshire.
  • January to October 1987. Visiting Research Scientist, Department of Microbiology and Molecular Genetics, University of California, Irvine, California,
  • 1982-1986 Molecular Biologist / Clinical Cytogeneticist,Centre for Human Genetics, Langhill, 117, Manchester Road, Sheffield.
  • 1979-1982 Research Scientist, University Department of Haematology, Royal Hallamshire Hospital, Sheffield.
Research interests

The role of GluR2 editing in motor neuron disease.


The AMPA receptor subunit GluR2 controls calcium entry to the motor neurone. During development it undergoes post transcriptional RNA editing giving rise to a mature form which blocks the receptor pore. There is evidence which suggests that in some forms of motor neurone disease this process is faulty giving rise to a receptor that becomes calcium permeable. In collaboration with colleagues in Edinburgh I am investigating this evidence in our own material.

Selective vulnerability to neurodegeneration of motor neuron populations.


In motor neuron disease there is a loss of motor neurons from the spinal cord and motor cortex but the motor neurons controlling eye movement (oculomotor) and the pelvic floor are spared. I am currently involved with a project to examine the gene expression profiles of both the spinal cord and oculomotor motor neurons in order to determine what mechanisms might be involved in the differential vulnerability of the two groups.

Microarray analysis of RNA extracted from formalin-fixed paraffin embedded samples.


There is a large archive of tissue samples collected for histological analysis that are formalin fixed and then embedded in paraffin. I have begun a project to develop a reliable methodology to unlock the RNA from these samples and make it available for microarray studies. This will enable us to carry out gene expression studies of some valuable samples in a number of areas.

Publications

Journal articles

Chapters

Conference proceedings papers

  • Appleby-Mallinder CS, Heath PR & Highley JR (2019) DNA Methylation in Amyotrophic Lateral Sclerosis. JOURNAL OF PATHOLOGY, Vol. 249 (pp S15-S15) RIS download Bibtex download
  • Vazquez-Villasenor I, Garwood CJ, Heath PR, Simpson JE & Wharton SB (2019) Investigating senescence activation in response to oxidative DNA damage in neurones in vitro. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 45 (pp 39-39) RIS download Bibtex download
  • Ameen-Ali KE, Simpson JE, Wharton SB, Heath PR, Sharp P & Berwick J (2017) Early cellular changes and cognitive decline in an Alzheimer's mouse model. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 43 (pp 42-43) RIS download Bibtex download
  • Vazquez-Villasenor I, Simpson JE, Garwood CJ, Ince PG, Heath PR & Wharton SB (2017) Neuronal senescence as a contributor to neurodegeneration. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 43 (pp 43-43) RIS download Bibtex download
  • Nafee T, Sanchez-Lopez JA, Heath PR, Ola B & Fazeli A (2015) The role of microRNAs in mediating a state of enhanced endometrial receptivity in response to trophoblast cells: an integrative analysis. HUMAN REPRODUCTION, Vol. 30 (pp 106-106) RIS download Bibtex download
  • Alsomali KY, Simpson J & Heath PR (2014) Investigating RNA editing in the pathogenesis of amyotrophic lateral sclerosis. FEBS JOURNAL, Vol. 281 (pp 685-686) RIS download Bibtex download
  • Al-Mashhadi SA, Simpson JE, Heath PR, Dickman MJ, Ince PG & Wharton SB (2014) Oxidative stress and DNA damage in cerebral white matter lesions of the ageing human brain. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 40 (pp 33-34) RIS download Bibtex download
  • Simpson JE, Ince PG, Matthews F, Shaw PJ, Heath PR, Brayne C, Garwood C, Abdalla H, Higginbottom A & Wharton SB (2014) A neuronal DNA damage response is detected at low Braak stages and correlates with cognitive impairment in the ageing brain. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 40 (pp 31-32) RIS download Bibtex download
  • Al-Mashhadi SA, Simpson JE, Heath PR, Dickman MJ, Ince PG & Wharton SB (2013) Oxidative stress and DNA damage in cerebral white matter lesions of the ageing human brain. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 39 (pp 36-37) RIS download Bibtex download
  • Waller R, Heath PR, Woodroofe MN, Francese S, Wharton SB, Ince PG, Sharrack B & Simpson JE (2012) Microarray analysis of the astrocyte transcriptome in normal appearing white matter in multiple sclerosis. JOURNAL OF NEUROIMMUNOLOGY, Vol. 253(1-2) (pp 54-54) RIS download Bibtex download
  • Green NH, Nicholls Z, Heath PR, Highley JR, Cooper-Knock J, Corfe BM, Wilson JH, Rattray M, Liu X, MacNeil S & Bury JP (2012) Barrett's Metaplasia: Exploiting a Tissue-engineered Model to Understand the Response of the Oesophageal Squamous Mucosa to Bile and Acid Exposure. JOURNAL OF PATHOLOGY, Vol. 228 (pp S3-S3) RIS download Bibtex download
  • Ferraiuolo L, Higginbottom A, Heath PR, Barber S, Greenald D, Kirby J & Shaw PJ (2012) DYSREGULATION OF THE CROSS-TALK WITH ASTROCYTES AS A CONTRIBUTORY FACTOR TO MOTOR NEURON INJURY IN MOTOR NEURON DISEASE. JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, Vol. 83(3) RIS download Bibtex download
  • Brockington A, Ning K, Heath P, Wood E, Malik K, Fusi N, Wharton S, Ince PG & Shaw PJ (2012) 156 Motor neurones subtypes resistant to degeneration in amyotrophic lateral sclerosis show distinct synaptic characteristics across species. Journal of Neurology, Neurosurgery & Psychiatry, Vol. 83(3) (pp e1.110-e1) RIS download Bibtex download
  • Simpson JE, Ince PG, Heath PR, Raman R, Shaw PJ, Gelsthorpe C, Baxter L, Forster G, Matthews F, Savva G , Brayne C et al (2011) Microarray analysis of the astrocyte transcriptome in the ageing brain: relationship to Alzheimer's pathology and ApoE genotype. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 37 (pp 33-33) RIS download Bibtex download
  • Highley R, Kirby J, Jansweijer JA, Heath PR, Shaw PJ & Ince PG (2011) TARDBP mutations, amyotrophic lateral sclerosis and alternative splicing. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, Vol. 37 (pp 20-20) RIS download Bibtex download
  • Simpson J, Ince P, Heath P, Raman R, Shaw P, Gelsthorpe C, Baxter L, Forster G, Matthews F, Savva G , Brayne C et al (2010) Microarray analysis of the astrocyte transcriptome in the ageing brain: relationship to Alzheimer's pathology and ApoE genotype. BRAIN PATHOLOGY, Vol. 20 (pp 46-46) RIS download Bibtex download
  • Brockington A, Heath P, Holden H, Kasher P, Autiero M, Claes F, Lambrechts D, Carmeliet P & Shaw PJ (2009) TRANSCRIPTIONAL PROFILING OF SPINAL MOTOR NEURONES IN THE VEGF(delta/delta) MOUSE MODEL OF AMYOTROPHIC LATERAL SCLEROSIS. JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, Vol. 80(11) RIS download Bibtex download
  • Cookson VJ, Waite SL, Heath PR, Milo M, Anumba DO & Chapman NR (2009) Identifying Regulatory Gene Networks in the Myometrium Governed by NF-kappa B. REPRODUCTIVE SCIENCES, Vol. 16(3) (pp 113A-113A) RIS download Bibtex download
  • Chico TJ, Milo M, Packham IM, Gray C, Heath PR, Hellewell PG, Ingham PW & Crossman DC (2007) Determining the transcriptional effect of shear stress in vivo. HEART, Vol. 93(2) RIS download Bibtex download
  • Blackburn D, Sargsyan S, Monk P, Shaw P, Heath P & Holden H (2007) The role of microglial cells in MND. NEURON GLIA BIOLOGY, Vol. 2 (pp S56-S56) RIS download Bibtex download

Datasets

Grants
  • YCR
  • MNDA
  • Sheffield Hospitals Charitable Trust