Dr Annica K.B. Gad

PhD

Department of Oncology and Metabolism

Lecturer in Cancer Biology

a.k.gad@sheffield.ac.uk
+44 114 215 9681

The Medical School

Full contact details

Dr Annica K.B. Gad
Department of Oncology and Metabolism
The Medical School
Beech Hill Road
Sheffield
S10 2RX

Profile

For enquiries, please contact - iicd-om-operational@sheffield.ac.uk

Annica K.B. Gad obtained her PhD in 2005 at the Karolinska Institutet in Stockholm, Sweden, studying “Cell Cycle Control by Components of Cell Anchorage” with Staffan Strömblad as her supervisor. Her postdoctoral work started with Mario Gimona at the Consorzio Mario Negri Sud Research Institute, Italy, focussing on the cytoskeletal crosslinking that underlies podosome formation. She then joined Pontus Aspenström at the Ludwig Institute for Cancer Research in Uppsala, Sweden, and also back at the Karolinska Institutet in Stockholm, where she worked on the biology of the Rho GTPases.

In 2012, Dr Gad obtained a position as a Senior Researcher at the Karolinska Institute for her studies of the molecular mechanisms that control the mechanical properties of single cells, particularly in relation to cancer. Then in 2017, Dr Gad was recruited to the University of Madeira in Portugal, where she continued to study the mechanical properties of cells as a Senior Researcher.

In 2019, she moved to a Lecturer position at the University of Sheffield.

Current PhD Opportunities

Research interests

Dr Gad’s research is aimed at the identification of the molecular mechanisms that govern cancer cell metastasis and the cancer-promoting ability of cancer-associated fibroblasts. Her approach is via the control of the mechanical properties of cells, with the further aim being the development of drugs against cancer metastasis. As such drugs will be based only on cell mechanics, they will represent a previously unknown, mechano-based, class of drugs. Her research is thus focussed on how regulators of the cytoskeleton control cellular contractile forces and cellular stiffness, through approaches that include bioengineering, biochemistry and advanced microscopy, such as super-resolution fluorescence microscopy, collodial probe atomic force microscopy, and traction force microscopy. Studies of the mechanical properties of cells require advanced equipment and expertise from different research fields, and therefore, by its very nature, her research is highly interdisciplinary. As tumour cell invasion and metastasis is the number one cause of cancer deaths, the identification of a previously unknown, mechano-based, class of target proteins for the development of drugs that block cell invasion has great promise to provide significantly improved prevention of human deaths due to cancers.

Current projects:

The Molecular Control of the Mechanical Properties of Human Cells and Cancer.

PhD project directory

https://www.sheffield.ac.uk/postgraduate/phd/scholarships/projects

Treating cancer by targeting the mechanical properties of cells

Department of Oncology and Metabolism PhD Research Project, Self Funded

Dr A Gad

Application Deadline: Applications accepted all year round.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Danielsson F, Peterson M, Caldeira Araújo H, Lautenschläger F & Gad A (2018) Vimentin diversity in health and disease. Cells, 7(10). View this article in WRRO
Xu L, Braun LJ, Rönnlund D, Widengren J, Aspenström P & Gad AKB (2018) Nanoscale localization of proteins within focal adhesions indicates discrete functional assemblies with selective force‐dependence. The FEBS Journal, 285(9), 1635-1652. View this article in WRRO
Alkasalias T, Alexeyenko A, Hennig K, Danielsson F, Lebbink RJ, Fielden M, Turunen SP, Lehti K, Kashuba V, Madapura HS , Bozoky B et al (2017) RhoA knockout fibroblasts lose tumor-inhibitory capacity in vitro and promote tumor growth in vivo. Proceedings of the National Academy of Sciences, 114(8), E1413-E1421. View this article in WRRO
Terriac E, Coceano G, Mavajian Z, Hageman TAG, Christ AF, Testa I, Lautenschläger F & Gad AKB (2017) Vimentin Levels and Serine 71 Phosphorylation in the Control of Cell-Matrix Adhesions, Migration Speed, and Shape of Transformed Human Fibroblasts. Cells, 6(1). View this article in WRRO
(2015) Correction to Supporting Information for Danielsson et al., Majority of differentially expressed genes are down-regulated during malignant transformation in a four-stage model. Proceedings of the National Academy of Sciences, 112(51), E7158-E7158.
Rönnlund D, Gad AKB, Blom H, Aspenström P & Widengren J (2013) Spatial organization of proteins in metastasizing cells. Cytometry Part A, 83(9), 855-865.
Danielsson F, Skogs M, Huss M, Rexhepaj E, O'Hurley G, Klevebring D, Ponten F, Gad AKB, Uhlen M & Lundberg E (2013) Majority of differentially expressed genes are down-regulated during malignant transformation in a four-stage model. Proceedings of the National Academy of Sciences, 110(17), 6853-6858.
Thullberg M, Gad A, Le Guyader S & Stromblad S (2007) Oncogenic H-Ras V12 promotes anchorage-independent cytokinesis in human fibroblasts. Proceedings of the National Academy of Sciences, 104(51), 20338-20343.
Gad A, Thullberg M, Dannenberg J-H, Riele HT & Strömblad S (2004) Retinoblastoma Susceptibility Gene Product (pRb) and p107 Functionally Separate the Requirements for Serum and Anchorage in the Cell Cycle G1-phase. Journal of Biological Chemistry, 279(14), 13640-13644.

All publications

Journal articles

Tonge JJ, Notley SV, Dunning MJ, López-Guajardo A, Medcalf JD, Heldin P, Panoutsos G & Gad AKB (2022) Hyaluronan nanoscale clustering and Hyaluronan synthase 2 expression are linked to the invasion of child fibroblasts and infantile fibrosarcoma in vitro and in vivo. Scientific Reports, 12, 1-10. View this article in WRRO
Shaebani MR, Stankevicins L, Vesperini D, Urbanska M, Flormann DAD, Terriac E, Gad AKB, Cheng F, Eriksson JE & Lautenschläger F (2022) Effects of Vimentin on the Migration, Search Efficiency, and Mechanical Resilience of Dendritic Cells. Biophysical Journal.
Rezk R, Marín-García R & Gad AKB (2021) The Fibrillar Matrix: Novel Avenues for Breast Cancer Detection and Treatment. Engineering, 7(10), 1375-1380.
Flormann DAD, Schu M, Terriac E, Thalla D, Kainka L, Koch M, Gad AKB & Lautenschläger F (2021) A novel universal algorithm for filament network tracing and cytoskeleton analysis.. FASEB J, 35(5), e21582.
Danielsson F, Peterson M, Caldeira Araújo H, Lautenschläger F & Gad A (2018) Vimentin diversity in health and disease. Cells, 7(10). View this article in WRRO
Xu L, Braun LJ, Rönnlund D, Widengren J, Aspenström P & Gad AKB (2018) Nanoscale localization of proteins within focal adhesions indicates discrete functional assemblies with selective force‐dependence. The FEBS Journal, 285(9), 1635-1652. View this article in WRRO
Alkasalias T, Alexeyenko A, Hennig K, Danielsson F, Lebbink RJ, Fielden M, Turunen SP, Lehti K, Kashuba V, Madapura HS , Bozoky B et al (2017) RhoA knockout fibroblasts lose tumor-inhibitory capacity in vitro and promote tumor growth in vivo. Proceedings of the National Academy of Sciences, 114(8), E1413-E1421. View this article in WRRO
Terriac E, Coceano G, Mavajian Z, Hageman TAG, Christ AF, Testa I, Lautenschläger F & Gad AKB (2017) Vimentin Levels and Serine 71 Phosphorylation in the Control of Cell-Matrix Adhesions, Migration Speed, and Shape of Transformed Human Fibroblasts. Cells, 6(1). View this article in WRRO
Fallqvist B, Fielden ML, Pettersson T, Nordgren N, Kroon M & Gad AKB (2016) Experimental and computational assessment of F-actin influence in regulating cellular stiffness and relaxation behaviour of fibroblasts. Journal of the Mechanical Behavior of Biomedical Materials, 59, 168-184.
Xu L, Rönnlund D, Aspenström P, Braun LJ, Gad AKB & Widengren J (2016) Resolution, target density and labeling effects in colocalization studies - suppression of false positives by nanoscopy and modified algorithms. FEBS Journal, 283(5), 882-898. View this article in WRRO
(2015) Correction to Supporting Information for Danielsson et al., Majority of differentially expressed genes are down-regulated during malignant transformation in a four-stage model. Proceedings of the National Academy of Sciences, 112(51), E7158-E7158.
Blom M, Reis K, Nehru V, Blom H, Gad AKB & Aspenström P (2015) RhoD is a Golgi component with a role in anterograde protein transport from the ER to the plasma membrane. Experimental Cell Research, 333(2), 208-219.
Rönnlund D, Xu L, Perols A, Gad AKB, Eriksson Karlström A, Auer G & Widengren J (2014) Multicolor Fluorescence Nanoscopy by Photobleaching: Concept, Verification, and Its Application To Resolve Selective Storage of Proteins in Platelets. ACS Nano, 8(5), 4358-4365.
Spielmann T, Xu L, Gad AKB, Johansson S & Widengren J (2014) Transient state microscopy probes patterns of altered oxygen consumption in cancer cells. FEBS Journal, 281(5), 1317-1332.
Rathje L-SZ, Nordgren N, Pettersson T, Rönnlund D, Widengren J, Aspenström P & Gad AKB (2014) Oncogenes induce a vimentin filament collapse mediated by HDAC6 that is linked to cell stiffness. Proceedings of the National Academy of Sciences, 111(4), 1515-1520. View this article in WRRO
Gad AKB, Rönnlund D, Widengren J & Aspenström P (2014) Analysis of Rho GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy, 339-357.
Rönnlund D, Gad AKB, Blom H, Aspenström P & Widengren J (2013) Spatial organization of proteins in metastasizing cells. Cytometry Part A, 83(9), 855-865.
Danielsson F, Skogs M, Huss M, Rexhepaj E, O'Hurley G, Klevebring D, Ponten F, Gad AKB, Uhlen M & Lundberg E (2013) Majority of differentially expressed genes are down-regulated during malignant transformation in a four-stage model. Proceedings of the National Academy of Sciences, 110(17), 6853-6858.
Jagodic M, Stridh P, Gad AKB, Paine A, Udekwu KI, Sjöholm LK, Svensson M & Pan-Hammarström Q (2013) Nurture your scientific curiosity early in your research career. Nature Genetics, 45(2), 116-118.
Gad AKB, Nehru V, Ruusala A & Aspenström P (2012) RhoD regulates cytoskeletal dynamics via the actin nucleation–promoting factor WASp homologue associated with actin Golgi membranes and microtubules. Molecular Biology of the Cell, 23(24), 4807-4819. View this article in WRRO
Gad AKB, Rönnlund D, Spaar A, Savchenko AA, Petranyi G, Blom H, Szekely L, Widengren J & Aspenström P (2012) Rho GTPases link cellular contractile force to the density and distribution of nanoscale adhesions. The FASEB Journal, 26(6), 2374-2382.
Gad AKB & Aspenström P (2010) Rif proteins take to the RhoD: Rho GTPases at the crossroads of actin dynamics and membrane trafficking. Cellular Signalling, 22(2), 183-189.
Gad A, Lach S, Crimaldi L & Gimona M (2008) Plectin deposition at podosome rings requires myosin contractility. Cell Motility and the Cytoskeleton, 65(8), 614-625.
Thullberg M, Gad A, Le Guyader S & Stromblad S (2007) Oncogenic H-Ras V12 promotes anchorage-independent cytokinesis in human fibroblasts. Proceedings of the National Academy of Sciences, 104(51), 20338-20343.
Thullberg M, Gad A, Beeser A, Chernoff J & Strömblad S (2007) The kinase-inhibitory domain of p21-activated kinase 1 (PAK1) inhibits cell cycle progression independent of PAK1 kinase activity. Oncogene, 26(12), 1820-1828.
Päll T, Gad A, Kasak L, Drews M, Strömblad S & Kogerman P (2004) Recombinant CD44-HABD is a novel and potent direct angiogenesis inhibitor enforcing endothelial cell-specific growth inhibition independently of hyaluronic acid binding. Oncogene, 23(47), 7874-7881.
Gad A, Thullberg M, Dannenberg J-H, Riele HT & Strömblad S (2004) Retinoblastoma Susceptibility Gene Product (pRb) and p107 Functionally Separate the Requirements for Serum and Anchorage in the Cell Cycle G1-phase. Journal of Biological Chemistry, 279(14), 13640-13644.
Bakhiet M, Tjernlund A, Mousa A, Gad A, Strömblad S, Kuziel WA, Seiger Å & Andersson J (2001) RANTES promotes growth and survival of human first-trimester forebrain astrocytes. Nature Cell Biology, 3(2), 150-157.
Kim HR, Warrington SJ, López-Guajardo A, Al Hennawi K, Cook SL, Griffith ZDJ, Symmes D, Zhang T, Qu Z, Xu Y , Chen R et al () ALD-R491 regulates vimentin filament stability and solubility, cell contractile force, cell migration speed and directionality. Frontiers in Cell and Developmental Biology, 10.
Evans CA, Kim HR, Macfarlane SC, Nowicki PIA, Baltes C, Xu L, Widengren J, Lautenschläger F, Corfe BM & Gad AKB () Metastasising Fibroblasts Show an HDAC6-Dependent Increase in Migration Speed and Loss of Directionality Linked to Major Changes in the Vimentin Interactome. International Journal of Molecular Sciences, 23(4), 1961-1961.

Conference proceedings papers

Nordgren N, Rathje L-S, Pettersson T, Ronnlund D, Widengren J, Aspenstrom P & Gad A (2015) Oncogene induced stiffening of living cells. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 249
Danielsson F, Lundberg E, Uhlen M & Gad AK (2014) Profiling the Molecular changes during malignant transformation and response to different oxygen levels, using a combined transcriptomics and proteomics approach. MOLECULAR BIOLOGY OF THE CELL, Vol. 25
Serebryannyy L, Parilla M, Laster K, Yu H-J, Blom H, Gad AK, Gottardi CJ, Kosak ST & de Lanerolle P (2013) Nuclear Actin Dynamics Regulate Nuclear Structure and Transcription. MOLECULAR BIOLOGY OF THE CELL, Vol. 24
Thullberg M, Gad A & Strömblad S (2007) 316 POSTER Oncogenic H-Ras V12 promotes anchorage-independent cytokinesis. European Journal of Cancer Supplements, Vol. 5(4) (pp 61-61)

Professional activities: 1. ASPIC - The Portuguese Association for Cancer Research
2. EACR - European Association for Cancer Research. EACR Ambassador since 2018.
3. COST action CA 15214, an Integrative Action for Multidisciplinary Studies on Cellular Structural Networks, Working Group number 4: “Mechanobiological principles of rare and common diseases”