Widefield Microscopes

Leica AF6000LX inverted microscope - Room DU12

Leica DMI4000B inverted microscope - Room DU12

AF6000LX This fully automated microscope primarily designed for live cell imaging. It is fitted with an environmental chamber maintained at 37°C, it is also equipped with a gas mixer for the supply of CO2 and a range of stage top incubators designed to fit various plates and dishes. The microscope can be used to acquire via Transmitted Light BrightField (TL-BF) or Phase contrast (TL-Ph) images. The microscope is fitted with appropriate filters for imaging a range of fluorophores.

  • Filter A4 is used for DAPI, Hoesch and other blue markers
  • Filter L5 for GFP, Alexa 488, FITC and other green markers
  • Filter N3 for TRITC, Alexa 543, 555 or 568 and other red markers
  • Filter Y3 for CY5, Topro, Alexa 647, Draq5 etc
  • Filter CHE for mCherry, Alexa 594 etc.

The N3 filter is not ideally suited to longer wavelength red fluorophores eg Alexa 594, mCherry etc but, well stained specimens with these markers can still be imaged. There is now a new CHE filter for mCherry or Alexa 594 which will work well with the A4 and L5 blue and green filters. However, there will be significant "cross talk" between images taken using either the N3 and CHE or CHE and Y3 filters.

The microscope control software has been upgraded to (LAS-X) which can support so called 6 dimensional imaging, xyz, time, colour and multiple locations (eg imaging every 10 minutes for 12 hours of transmitted phase contrast and GFP with 4 focus levels in each well of a 24 well plate =13824 images). The motorised stage can also be used to acquire “tiled” images. This microscope can also collect "tiled" images which can cover quite substantial areas of the slide or dish.
The microscope is fitted with 5x/0.12, 10x/0.3 Ph1, 20x/0.35 Ph1, 40x/0.6 Ph2 and 63x/0.7 Ph2 dry objectives which are all designed to work with tissue culture flasks, dishes and plates.

A 20x/0.7 Ph2 dry lens and a 100x/1.4 Oil immersion lens are also available but these can only be used with thin glass bottomed plates/dishes or specimens mounted under thin glass coverslips. These lenses are only available on a "first come first served" basis and must be returned to the shelf above the AF6000 computer at the end of the session. Please ensure that the normal lens is replaced or a plug is fitted into the microscope turret after removing any lens. Take care to ensure that dry lenses do not become contaminated with oil when using the 100x/1.4 lens. Only use lens tissue (never ordinary tissue or paper towel) when removing oil from lenses. Only use the oil provided and do not mix with oil from other sources.

DMI4000B This microscope is very similar to the AF6000 and has the same software control (LASAF) but, it is not automated. It is ideally suited to imaging fixed cells/tissue in culture plates or on slides. The microscope is fitted with a dual mode camera, black and white for fluorescence and colour for histochemical stained specimens (eg Haemotoxilin & Eosin, DAB etc), either mode can be used for brightfield or phase contrast imaging with transmitted light.  When using Phase Contrast imaging the "phase ring" in the condenser should be positioned as follows:

  • For the standard 10x/0.30 lens  use position number 7
  • For the standard 20x/0.35 lens use position number 7
  • For the special 20x/0.70 lens use position number 1
  • For the standard 40x/0.60 lens use position number 1
  • For the standard 63x/0.70 lens use position number 1
  • For bright field (no contrast enhancement) number 6 or 2

Fluorescence excitation is provided by a CoolLed LED light source (so no warm up/cool down time). Filters appropriate for imaging a range of fluorophores are fitted. 

  • Filter A is used for "live/dead" assays but can be used for images when the camera is working in colour mode.
  • Filter A4 is used for DAPI, Hoesch and other blue markers
  • Filter L5 for GFP, Alexa 488, FITC and other green markers
  • Filter N3 for TRITC, Alexa 543, 555 or 568 and other red markers
  • Filter BGR is not really suitable for taking images but can be used when the camera is working in colour mode.

The filters are not ideally suited to longer wavelength red fluorophores eg Alexa 594, mCherry etc but, well stained specimens with these markers can still be imaged. Far red markers such as CY5, Alexa 647 etc will not work on this microscope as the camera is not sensitive to these wavelengths. 

The microscope is fitted with 2.5x/0.07, 5x/0.15, 10x/0.3Ph1, 20x/0.35Ph1, 40x/0.6/Ph2 dry objectives as standard, higher magnifications may be available.

A 20x/0.7 Ph2 dry lens and a 100x/1.4 Oil immersion lens are also available but these can only be used with thin glass bottomed plates/dishes or specimens mounted under thin glass coverslips. These lenses are only available on a "first come first served" basis and must be returned to the shelf above the AF6000 computer at the end of the session. Please ensure that the normal lens is replaced or a plug is fitted into the microscope turret after removing any lens. Take care to ensure that dry lenses do not become contaminated with oil when using the 100x/1.4 lens. Only use lens tissue (never ordinary tissue or paper towel) when removing oil from lenses. Only use the oil provided and do not mix with oil from other sources.

Olympus IX73 inverted microscope - Room Jessop JB4, 65

Inverted Olympus IX73 microscopeThis microscope is primarily designed for tissue culture and general lab microscopy can be used to image cells and tissue using transmitted light with either phase contrast or epifluorescence. It can also be used for time-lapse imaging but at present only with specimens requiring room temperature. Fluorescence excitation is provided by a CoolLed LED light source suitable for imaging Blue (eg DAPI), Green (eg ALexa488, GFP, FITC etc) and Red (eg Alexa 543, 555 or 568, TRITC, Texas Red etc). The microscope has fairly simple to use control software (Micromanager). 

The microscope is fitted with 10x/0.3 UPlanFLNPh1, 20x/0.5 UPlanFLN Ph1, 40x/0.75 UPlanFLN Ph2 and 63x/0.7 Ph2 dry objectives which are all designed to work with glass slides, tissue culture flasks, plates etc and a 60x/1.35 UPlan S apo-chromatic oil immersion lens which can only be used with thin glass, bottomed plates/dishes or specimens mounted under thin glass coverslips. 

 Take care to ensure that dry lenses do not become contaminated with oil when using the 63x/1.35 lens. Only use lens tissue (never ordinary tissue or paper towel) when removing oil from lenses. Only use the oil provided on the shelf above and do not mix with oil from other sources.