Kroto Research Institute Confocal and Two-Photon Imaging Facility
This facility is located within the Kroto Research Institute and is part of the instrumentation available for Imaging and Analysis within the Centre for Biomaterials and Tissue Engineering.
The facility contains two Zeiss LSM510 Meta confocal microscopes which can be booked and operated independently of each other. Both microscopes have their own set of lasers and both can also be connected to a tuneable two-photon Ti-Sapphire laser.
There is an offline PC, running the Zeiss image analysis software, which can be booked for data analysis.
There is also a Leica inverted epifluorescent microscope with an environmental chamber and camera.
This open access facility is available for use by researchers based in the Kroto Institute, other users from within Sheffield University and external researchers from both academia and industry. For further details on the theory behind confocal imaging, the facilities available, how to book the facilities or register as a new user please see the links.
For further information or to discuss your requirements in more detail, please contact Dr Nicola Green
Publishing Images Obtained Using the Kroto Imaging Facility
If you would like to incorporate any images obtained within the Kroto Imaging Facility into your published work, please could you ensure that you acknowledge the Kroto Facility within the publication. For advice on how to do this, together with some suggested wording for use in the Materials and Methods section of a publication please follow the link to the Publishing Images webpage.
There is also a list of recent publications that include images or data obtained using the Kroto Imaging Facility. If you have a relevant recent publication which is not included in this list, please send the details to Dr Nicola Green.
Confocal Facility Hands-on Imaging Course
Do you have little or no experience of using a confocal microscope?
Would you like to learn some basic background theory in relation to confocal microscopy, but don't need an indepth understanding of the physics?
Do you want to know how to use the confocal microscope to image samples, particularly of a biological nature?
One day hands-on imaging courses are run at the Kroto, for people within the University of Sheffield.
The cost of this course is £50.
Numbers are limited to 6 to ensure everyone gets full access to the equipment and places are allocated on a first come basis.
To register or for more information contact Dr Nicola Green
Please note the latest course has now taken place, however if you are interested in attending a similar course at a later date, please contact Dr Green for a registration form and to be added to the waiting list.