Flow Cytometry Core Service

Overview

The Medical School Flow Cytometry Core Facility is a resource available for all in the University. The lab comprises of 5 analysers and 1 cell sorter, and is supported by two dedicated members of Technical staff.

Services provided by the facility

Experimental design – including advice regarding controls, dyes, fluorochrome choice, cell number etc

Training – users are trained to run samples independently on the analysers *for an extra fee, samples can be run for users by Flow Cytometry staff

Analysis – advice can be provided to assist with data analysis

Machines available

Analysers

2 x FACSCalibur - 2 lasers 4 detectors. Tube loading only.
Attune Autosampler - 2 lasers 6 detectors. Tube and 96 well plate
LSRII - 4 lasers 13 detectors. Tube loading only.
FACSArray - 2 lasers 4 detectors. 96 well plate loading only for CBA assays

Sorter

FACSAria IIu - 3 lasers 9 detectors. Tube loading only

Miscalleaneous

Countess - automated cell counter
gentleMACS Dissociator - automated tissue dissociator seperating organs and tissues into sterile single cell suspensions

Attune and Gentle MACS