Dr Daniel Humphreys

Dan Humphreys

Lecturer
Department of Biomedical Science
University of Sheffield
Western Bank
Sheffield S10 2TN
United Kingdom

Room: C 08 Florey building
Telephone: +44(0) 114 222 4632
Email: d.humphreys@sheffield.ac.uk

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Overview

Career History

  • 2016: Lecturer, Department of Biomedical Science, The University of Sheffield
  • 2014 - 2016: Research Fellow and Principal Investigator, University of Cambridge
  • 2008 - 2013: Postdoctoral Research Associate, University of Cambridge
  • 2003 - 2007: PhD, University of Cambridge
  • 2002 - 2003: Masters of Biological Research, University of Manchester
  • 1999 - 2002: Bachelors of Science, University of Liverpool

Research Interests

Manipulation of mammalian host cell biology by bacterial toxins and virulence effectors.

Research

Manipulation of mammalian host cell biology by bacterial toxins and virulence effectors

To establish infections within mammalian hosts, bacterial pathogens have evolved sophisticated strategies to exploit host cell biology. They deploy toxins and virulence effector proteins into our cells that hijack cellular processes to promote pathogen survival, replication and dissemination, and interfere with the host immune response.

We are interested in the cell biology subverted by bacterial pathogens and how this ultimately contributes to diseases that can have devastating consequences to the health of humans and animals. Understanding host-pathogen interactions is especially important when considering the human health threat posed by many bacterial pathogens that continue to develop multidrug resistance.

Bacterial manipulation of host cell biology

Bacterial pathogens are known to inject virulence effectors that hijack the actin cytoskeleton. For example, enteropathogenic and enterohaemorrhagic Escherichia coli generate ‘actin pedestals’ to colonise the host cell surface whilst Salmonella and Shigella invade cells by generating ‘membrane ruffles’.

Once inside cells, Salmonella survives within a vacuole and ‘evades destruction within lysosomes’. Shigella on the other hand uses ‘actin-based propulsion to ‘’rocket’ through the cell and even propel itself into uninfected neighbouring cells for dissemination. Salmonella, E.coli and Shigella also secrete cytolethal distending toxins that cause DNA damage to induce ‘cell cycle arrest’ and interfere with immune cell signalling.

Opportunities

Positions available

There are currently no positions available in the lab. However, we always welcome speculative applications.

To express interest, please feel free to email me your CV and research interests.

Email: d.humphreys@sheffield.ac.uk


PhD Opportunities

Selected publications

Journal articles