The University of Sheffield
Chemical and Biological Engineering

Dr Mark Dickman

Reader in Bioanalytical Science and Engineering
Director of Marketing and Recruitment

Contact

T: +44 (0)114 222 7541
F: +44 (0)114 222 7501

E: m.dickman@sheffield.ac.uk

Dr DickmanBiography

I obtained a First Class Honours Degree in Biochemistry/Chemistry at the University of Sheffield. I continued at the Krebs Institute, where I obtained my PhD. Following my PhD I joined a biotechnology company, Transgenomic LTD where I worked as a research scientist developing analytical techniques including DNA/RNA Chromatography.

Since joining the Department of Chemical and Biological Engineering at the University of Sheffield, my research has focused on the development and application analytical techniques to study biological systems. In particular, biological mass spectrometry in conjunction with bioseparations have been utilised to study a wide variety of biological systems. Using these analytical approaches we are interested in identifying and characterising protein complexes, protein-RNA/DNA complexes, protein post translational modifications and RNA post transcriptional modifications.

Research Interests

  • Biological mass spectrometry
  • Bioseparations
  • Post-translational and post-transcriptional modifications
  • Proteomics
  • CRISPR systems

Links

Teaching

  • CPE 2003 Separation Processes
  • CPE 221 Biochemistry

md

Recent Publications

Quantitative proteomic analysis of intracytoplasmic membrane development in Rhodobacter sphaeroides. Jackson PJ, Lewis HJ, Tucker JD, Hunter CN, Dickman MJ. Mol Microbiol. 2012, 84(6):1062-78.

Rapid Resonance Raman micro-spectroscopy to probe carbon dioxide fixation by single cells in microbial communities. Li M, Canniffe DP, Jackson PJ, Davison PA, FitzGerald S, Dickman MJ, Burgess GJ, Hunter CN, Huang WE. ISME J. 2012 (4):875-85.

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Cruz A, Hautbergue GM, Artymiuk PA, Baker PJ, Bokori-Brown M, Chang CT, Dickman MJ, Phillips HL, Essex-Lopresti A, Harding SV, Mahadi NM, Marshall LE, Mohamed R, Nathan S,. Ngugi SA, Ong C, Ooi WF, Partridge LJ, Raih MF, Ruzhenikov S, Sakar-Tyson M, Sedelnikova SE, Smither SJ, Tan P, Titball RW, Wilson SA, Rice DW. Science, 2011, 334 (6057) 821-824.

Characterisation and quantification of RNA post-transcriptional modifications using stable isotope labeling of RNA in conjunction with mass spectrometry analysis. Waghmare SP, Dickman MJ. Anal Chem. 2011, 83(12):4894-901.

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions. Wiedenheft B, van Duijn E, Bultema J, Waghmare S, Zhou K, Barendregt A, Westphal W, Heck A, Boekema E, Dickman M, Doudna JA. Proc Natl Acad Sci U S A., 2011, 108(25):10092-7.

Structural basis for CRISPR RNA-guided DNA recognition by Cascade. Jore MM, Lundgren M, van Duijn E, Bultema JB, Westra ER, Waghmare SP, Wiedenheft B, Pul U, Wurm R, Wagner R, Beijer MR, Barendregt A, Zhou K, Snijders AP, Dickman MJ, Doudna JA, Boekema EJ, Heck AJ, van der Oost J, Brouns SJ. . Nat Struct Mol Biol. 2011, May;18(5):529-36.

Analysis of arginine and lysine methylation utilizing peptide separations at neutral pH and electron transfer dissociation mass spectrometry. Snijders AP, Hung ML, Wilson SA, Dickman MJ. J Am Soc Mass Spectrom. 2010 (1):88-96. Epub 2009 Sep 20.

Studying the mechanism of RNA separations using RNA chromatography and its application in the analysis of ribosomal RNA and RNA:RNA interactions. Waghmare SP, Pousinis P, Hornby DP, Dickman MJ. J Chromatogr A. 2009 Feb 27;1216(9):1377-82. Epub 2008 Dec 30.

Shotgun proteome analysis utilising mixed mode (reversed phase-anion exchange chromatography) in conjunction with reversed phase liquid chromatography mass spectrometry analysis.Phillips HL, Williamson JC, van Elburg KA, Snijders AP, Wright PC, Dickman MJ. Proteomics. 2010 Aug;10(16):2950-60.

Small CRISPR RNAs guide antiviral defense in prokaryotes. Brouns SJ, Jore MM, Lundgren M, Westra ER, Slijkhuis RJ, Snijders AP, Dickman MJ, Makarova KS, Koonin EV, van der Oost J. Science. 2008 Aug 15;321(5891):960-4.

Post-translational modifications of the linker histone variants and their association with cell mechanisms. Wood C, Snijders A, Williamson J, Reynolds C, Baldwin J, Dickman M. (2009) FEBS J. 2009 Jul;276(14):3685-97.

Characterization of post-translational modifications of the linker histones H1 and H5 from chicken erythrocytes using mass spectrometry. Snijders AP, Pongdam S, Lambert SJ, Wood CM, Baldwin JP, Dickman MJ. J Proteome Res. 2008 (10):4326-35. Epub 2008 Aug 28.

Enrichment and analysis of RNA centred on ion pair reverse phase methodology. Dickman, MJ. and Hornby, DP. RNA. 2006, 12;691-6.