Chromatography

ChromatographyThe Chromatography Service is an analytical facility based in the Department of Chemistry and is offered to all University of Sheffield departments, as well as external customers.

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Chromatography is a technique for separating and analysing chemical mixtures and the department's service comprises three main parts.

The first two are:

  • High Performance Liquid Chromatography (HPLC) with either single wavelength, photodiode array, evaporative light scattering or differential refractive index detectors.
  • Gas Chromatography (GC) with either flame ionisation or thermal conductivity detectors.

Both HPLC and GC require a minimum of 2mg's of sample for routine analysis.

Peaks can be identified by running reference materials and quantitative analysis can be achieved by preparing standards. A range of stationary phases are available including chiral columns.

We also offer:

  • Gel Permeation Chromatography (GPC) with differential refractive index detection, comparative molecular weight averages (from 500 to 2,000,000) can be determined using a range of columns and mobile phases calibrated with polystyrene standards. A minimum of 5mg's is required for analysis.

Techniques

Prices and turnaround times for the Chromatography Service are available by contacting Rob Hanson.

University of Sheffield customers should bring their samples along with a completed Internal service form and purchase order form (members of the Department of Chemistry do not need a purchase order form) to lab C43 in the Dainton Building. 

External customers should send their samples along with a completed External service form, safety information and purchase order form. 

Contact: Rob Hanson, R.J.Hanson@sheffield.ac.uk