Dr Timothy D. Craggs
Lecturer in Biological Chemistry
Tel: +44-(0)114-22-29347
Fax: +44-(0)114-22-29346
email: t.craggs@sheffield.ac.uk
Biographical Sketch
Dr Tim Craggs obtained his MSci in Chemistry from the University of Cambridge in 2002. After his PhD (Cambridge 2007) and a postdoc in St Andrews, he took up a Lindemann Fellowship at Yale University (2010), followed by senior postdoc positions at Oxford (Kapanidis Lab) and Bristol (Dillingham Lab). In 2016 he was appointed to a Lectureship in Chemical Biology at the University of Sheffield.
Research Keywords
Single molecule Spectroscopy
Teaching Keywords
Biological Chemistry
Selected Publications:
- Cool and dynamic: single-molecule fluorescence-based structural biology.
Craggs TD, Nature Methods, 14(2), 123-124, (2017). - Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters.
Nott TJ, Craggs TD & Baldwin AJ, Nature Chemistry, 8(6), 569-575, (2016). - DNA and Protein Requirements for Substrate Conformational Changes Necessary for Human Flap Endonuclease-1-catalyzed Reaction.
Algasaier SI, Exell JC, Bennet IA, Thompson MJ, Gotham VJB, Shaw SJ, Craggs TD, Finger LD & Grasby JA, Journal of Biological Chemistry, 291(15), 8258-8268, (2016). - Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles.
Nott TJ, Petsalaki E, Farber P, Jervis D, Fussner E, Plochowietz A, Craggs TD, Bazett-Jones DP, Pawson T, Forman-Kay JD & Baldwin AJ, Molecular Cell, 57(5), 936-947, (2015). - Alternating-laser excitation: single-molecule FRET and beyond.
Hohlbein J, Craggs TD & Cordes T, Chem. Soc. Rev., 43(4), 1156-1171, (2014). - Single-molecule characterization of Fen1 and Fen1/PCNA complexes acting on flap substrates.
Craggs TD, Hutton RD, Brenlla A, White MF & Penedo JC, Nucleic Acids Research, 42(3), 1857-1872, (2014). - Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion.
Hohlbein J, Aigrain L, Craggs TD, Bermek O, Potapova O, Shoolizadeh P, Grindley NDF, Joyce CM & Kapanidis AN, Nature Communications, 4, (2013). - Six steps closer to FRET-driven structural biology.
Craggs TD & Kapanidis AN, Nature Methods, 9(12), 1157-1158, (2012). - PCNA and XPF cooperate to distort DNA substrates.
Hutton RD, Craggs TD, White MF & Penedo JC, Nucleic Acids Research, 38(5), 1664-1675, (2010). - Green fluorescent protein: structure, folding and chromophore maturation.
Craggs TD, Chemical Society Reviews, 38(10), 2865-2865, (2009).
Journal articles
- Bennet I, Finger LD, Baxter NJ, Ambrose B, Hounslow A, Thompson MJ, Exell JC, Shahari NNBM, Craggs TD, Waltho JP & Grasby JA (2018) Regional conformational flexibility couples substrate specificity and scissile phosphate diester selectivity in human flap endonuclease 1. Nucleic Acids Research, 46, 5618-5633. View this article in WRRO
- Hellenkamp B, Schmid S, Doroshenko O, Opanasyuk O, Kühnemuth R, Rezaei Adariani S, Ambrose B, Aznauryan M, Barth A, Birkedal V, Bowen ME, Chen H, Cordes T, Eilert T, Fijen C, Gebhardt C, Götz M, Gouridis G, Gratton E, Ha T, Hao P, Hanke CA, Hartmann A, Hendrix J, Hildebrandt LL, Hirschfeld V, Hohlbein J, Hua B, Hübner CG, Kallis E, Kapanidis AN, Kim JY, Krainer G, Lamb DC, Lee NK, Lemke EA, Levesque B, Levitus M, McCann JJ, Naredi-Rainer N, Nettels D, Ngo T, Qiu R, Robb NC, Röcker C, Sanabria H, Schlierf M, Schröder T, Schuler B, Seidel H, Streit L, Thurn J, Tinnefeld P, Tyagi S, Vandenberk N, Vera AM, Weninger KR, Wünsch B, Yanez-Orozco IS, Michaelis J, Seidel CAM, Craggs TD & Hugel T (2018) Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study. Nature Methods, 15(9), 669-676. View this article in WRRO
- Fisher GLM, Pastrana CL, Higman VA, Koh A, Taylor JA, Butterer A, Craggs TD, Sobott F, Murray H, Crump MP, Moreno-Herrero F & Dillingham M (2017) The structural basis for dynamic DNA binding and bridging interactions which condense the bacterial centromere. eLife, 6. View this article in WRRO
- Craggs TD (2017) Cool and dynamic: single-molecule fluorescence-based structural biology. Nature Methods, 14(2), 123-124.
- Meli M, Sustarsic M, Craggs TD, Kapanidis AN & Colombo G (2016) DNA Polymerase Conformational Dynamics and the Role of Fidelity-Conferring Residues: Insights from Computational Simulations. Frontiers in Molecular Biosciences, 3. View this article in WRRO
- Nott TJ, Craggs TD & Baldwin AJ (2016) Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters. Nature Chemistry, 8(6), 569-575.
- Algasaier SI, Exell JC, Bennet IA, Thompson MJ, Gotham VJB, Shaw SJ, Craggs TD, Finger LD & Grasby JA (2016) DNA and Protein Requirements for Substrate Conformational Changes Necessary for Human Flap Endonuclease-1-catalyzed Reaction. Journal of Biological Chemistry, 291(15), 8258-8268. View this article in WRRO
- Evans GW, Hohlbein J, Craggs T, Aigrain L & Kapanidis AN (2015) Real-time single-molecule studies of the motions of DNA polymerase fingers illuminate DNA synthesis mechanisms. Nucleic Acids Research, 43(12), 5998-6008.
- Nott T, Petsalaki E, Farber P, Jervis D, Fussner E, Plochowietz A, Craggs TD, Bazett-Jones D, Pawson T, Forman-Kay J & Baldwin A (2015) Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles. Molecular Cell, 57(5), 936-947.
- Blouin S, Craggs TD, Lafontaine DA & Penedo JC (2015) Functional studies of DNA-protein interactions using FRET techniques, 1334, 115-141.
- (2014) Additions and corrections published 30th October 2013 to 15th July 2014. Chemical Society Reviews, 43(17), 6470-6470.
- Hohlbein J, Craggs TD & Cordes T (2014) Alternating-laser excitation: single-molecule FRET and beyond. Chem. Soc. Rev., 43(4), 1156-1171.
- Craggs TD, Hutton RD, Brenlla A, White MF & Penedo JC (2014) Single-molecule characterization of Fen1 and Fen1/PCNA complexes acting on flap substrates. Nucleic Acids Research, 42(3), 1857-1872. View this article in WRRO
- Hohlbein J, Craggs TD & Cordes T (2014) Alternating-laser excitation: single-molecule FRET and beyond (vol 43, pg 1156, 2014). CHEMICAL SOCIETY REVIEWS, 43(17), 6472-6472.
- Robb NC, Cordes T, Hwang LC, Gryte K, Duchi D, Craggs TD, Santoso Y, Weiss S, Ebright RH & Kapanidis AN (2013) The Transcription Bubble of the RNA Polymerase–Promoter Open Complex Exhibits Conformational Heterogeneity and Millisecond-Scale Dynamics: Implications for Transcription Start-Site Selection. Journal of Molecular Biology, 425(5), 875-885.
- Hohlbein J, Aigrain L, Craggs TD, Bermek O, Potapova O, Shoolizadeh P, Grindley NDF, Joyce CM & Kapanidis AN (2013) Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion. Nature Communications, 4(1). View this article in WRRO
- Craggs TD & Kapanidis AN (2012) Six steps closer to FRET-driven structural biology. Nature Methods, 9(12), 1157-1158.
- Hutton RD, Craggs TD, White MF & Penedo JC (2010) PCNA and XPF cooperate to distort DNA substrates. Nucleic Acids Research, 38(5), 1664-1675. View this article in WRRO
- Craggs TD (2009) Green fluorescent protein: structure, folding and chromophore maturation. Chemical Society Reviews, 38(10), 2865-2865.
- Blouin S, Craggs TD, Lafontaine DA & Penedo JC (2009) Functional studies of DNA-protein interactions using FRET techniques. Methods in Molecular Biology, 543, 475-502.
- Orte A, Craggs TD, White SS, Jackson SE & Klenerman D (2008) Evidence of an Intermediate and Parallel Pathways in Protein Unfolding from Single-Molecule Fluorescence. Journal of the American Chemical Society, 130(25), 7898-7907.
- Kuprov I, Craggs TD, Jackson SE & Hore PJ (2007) Spin Relaxation Effects in Photochemically Induced Dynamic Nuclear Polarization Spectroscopy of Nuclei with Strongly Anisotropic Hyperfine Couplings. Journal of the American Chemical Society, 129(29), 9004-9013.
- Huang J-R, Craggs TD, Christodoulou J & Jackson SE (2007) Stable Intermediate States and High Energy Barriers in the Unfolding of GFP. Journal of Molecular Biology, 370(2), 356-371.
- Jackson SE, Craggs TD & Huang J-R (2006) Understanding the folding of GFP using biophysical techniques. Expert Review of Proteomics, 3(5), 545-559.
- Khan F, Kuprov I, Craggs TD, Hore PJ & Jackson SE (2006) 19 F NMR Studies of the Native and Denatured States of Green Fluorescent Protein. Journal of the American Chemical Society, 128(33), 10729-10737.
Conference proceedings papers
- Fisher GLM, Bateman BC, Craggs TD & Dillingham MS (2018) The Conformational Landscape of SMC: A FRET Study. BIOPHYSICAL JOURNAL, Vol. 114(3) (pp 209A-209A)
- Craggs TD, Sustarsic M, Mosayebi M, Kaju H, Hohlbein J, Biggin PC, Doye JPK & Kapanidis AN (2016) How Structure-Specific DNA-Binding Proteins Recognise their Substrates. Biophysical Journal, Vol. 110(3) (pp 514a-515a)
- Craggs TD, Sustarsic M, Hohlbein J, Cuthbert A, Taylor N, Evans G & Kapanidis AN (2014) A Novel FRET-Based Structure of DNA Polymerase Complexed with Kinked Gapped-DNA. BIOPHYSICAL JOURNAL, Vol. 106(2) (pp 273A-273A)
- Orte A, Craggs TD, White SS, Jackson SE & Klenerman D (2007) YFP unfolding kinetics studied in a single-molecule nano-flow cell. BIOPHYSICAL JOURNAL (pp 660A-660A)