Dr Zoltán Kis

School of Chemical, Materials and Biological Engineering

Senior Lecturer (Associate Professor)

Zoltan Kis
Profile picture of Zoltan Kis
z.kis@sheffield.ac.uk

Full contact details

Dr Zoltán Kis
School of Chemical, Materials and Biological Engineering
G58
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD
Profile

I am a Senior Lecturer (Associate Professor) in the School of Chemical, Materials and Biological Engineering at The University of Sheffield, and an Honorary Lecturer at the Department of Chemical Engineering, Imperial College London.

I am leading a multidisciplinary team that is innovating and digitalising RNA vaccine and therapeutics production platform technologies. My work is addressing the challenges of producing large volumes of RNA-based vaccines and therapeutics, rapidly, at high quality and at low cost in a disease-agnostic manner.

I previously worked as a Research Associate in the Future Vaccine Manufacturing Hub at Imperial College London. I obtained my Ph.D. in Bioengineering from Imperial College London, UK, hold an M.Sc. in Applied Biotechnology and a B.Eng. in Chemical with Biochemical Engineering.

Research interests

Our exciting research combines experimental and computational modelling techniques to innovate RNA vaccine and therapeutics production multi-product platform processes. On the experimental front, we are developing, intensifying and scaling up continuous mRNA enzymatic synthesis, purification and formulation processes, alongside developing bespoke manufacturing equipment. Our modelling work streamlines process development and deploys software-based sensors and digital twins for monitoring and automating multi-product mRNA manufacturing. Our work is embedded into the Quality-by-Digital-Design framework and follows techno-economic considerations. Our efforts will enable the rapid development and mass manufacturing of high-quality mRNA vaccines/therapeutics at low costs against a wide range of diseases.

Key research areas include:

  • Experimental mRNA vaccine and RNA therapeutics manufacturing process development: process intensification, continuous manufacturing and scale-up.
  • Development and optimisation of continuous in vitro transcription (IVT) reaction for mRNA synthesis, continuous mRNA downstream purification processes based on continuous chromatography and tangential flow filtration unit operations, as well as continuous LNP formulation unit operations.
  • Development of new unit operations and design of process equipment for mRNA manufacturing.
  • Process digitalisation: development of soft sensors and digital twins to monitor and control the manufacturing of RNA vaccines, RNA therapeutics and other biopharmaceuticals.
  • Techno-economic modelling for reducing the costs, increasing production rates and increasing production volumes of RNA vaccines, RNA therapeutics and other biopharmaceuticals.
  • Quality by Digital Design for consistently ensuring product quality, support scale-up, technology transfer, and for accelerating the regulatory approval process.
Publications

Journal articles

Chapters

Preprints

Research group

For the latest info on our research and team, please visit our research team website.

Grants
DateFunderRoleTitleFunding amount

1 Oct 2024

– 

30 Sep 2027

Coalition for Epidemic Preparedness Innovations (CEPI)Principal InvestigatorRNAboxTM: An automated continuous closed RNA platform process-in-a-box for rapid outbreak-response disease-agnostic RNA vaccine development and mass-manufacturing at high-quality and low-cost

£3.7 million

(US $4. 8 million)

May 2024 – 

May 2026

Biotechnology and Biological Sciences Research Council (BBSRC). BB/Y007514/1. Engineering Biology, Mission Awards.Co-InvestigatorElectrospun mucoadhesive matrices for polymersome-mediated mRNA vaccine delivery£1.72 million

Nov 2023

Nov 2028

UK Department of Health and Social Care and Engineering and Physical Sciences Research Council (EPSRC)Co-InvestigatorUK-south-east Asia-vaccine manufacturing research hub£7.6 million

Oct 2023 –

Oct 2025

Innovate UK.Principal InvestigatorAutomated and digitalised RNA process-in-a-box for rapid outbreak-responsedisease-agnostic RNA vaccine/therapeutic development and manufacturing at high-quality and low cost£2 million
7 June 2023BBSRCCo-InvestigatorNanoAnalyzer: An emerging technology to analyse life at the nanoscale£242,400
20 October 2022.BBSRC Doctoral Training (CDT/DTC/DTP).Co-SupervisoriCASE PhD studentship with AstraZeneca Plc£84,000
March 2022.ESPRC Doctoral Training PartnershipCo-SupervisoriCASE PhD studentship with RedShift BioAnalytics, Inc.£130,200

Dec 2021 –

Jul 2022

United Kingdom Research and Innovation (UKRI), Higher Education Innovation Fund (HEIF)Co-InvestigatorInnovating the process of RNA vaccine and therapeutic manufacturing – building external partnerships and exploring commercialisation opportunities£74,331

Dec 2021 –

Oct 2024

Wellcome Leap RNA Readiness + Response (R3)Principal InvestigatorDigitalised, small-scale and high-throughput process for distributed and automated RNA production for therapy and pandemic preparedness.Multi-million USD (confidential)
2020-2022UKRI, EPSRC, EP/V01479X/1Co-applicantMeeting the UK demand for COVID19/SARS-CoV-2 vaccines via integrated manufacturing and supply chain optimisation£448,148
2020-2022Wellcome Innovator AwardCo-applicantA pathway to a live-attenuated whole parasite malaria vaccine.£727,458
2020-2021Kidney Research UK. Paediatric Innovation Grant AwardCo-InvestigatorCell catcher: developing a new method for cell isolation and preservation from urine samples of Bardet-Biedl Syndrome patients£40,000
Teaching activities
  • I am a Fellow of the Higher Education Academy and I am passionate about teaching and training the next generation of chemical and biological engineers.
  • I am supervising and co-supervising the PhD projects in the field of mRNA production, purification, formulation and analysis in our research team.
  • I am teaching the following modules: 1) CPE336-CPE6043, Biopharmaceutical Manufacturing, 3rd Year UG & M.Sc., Module Leader & Lecturer 2) BIE103 Introduction to Bioengineering, 1st Year UG, Lecturer
Professional activities and memberships
  • July 2019 – Present. American Institute of Chemical Engineers (AIChE), Postdoctoral Member.
  • Nov 2018 – Present. Institution of Chemical Engineers, London (IChemE), U.K., Professional Member.
  • June 2018 – Present. European Federation of Biotechnology (EFB).
  • June 2018 – Present. European Biotechnology Network (EBN).
  • June 2018 – Present. Developing Countries Vaccine Manufacturers Network (DCVMN).
  • May 2018 – Present. The Network in Bioprocessing (BioProNET), a BBSRC Network in Industrial Biotechnology and Bioenergy co-sponsored by EPSRC (U.K. based).
  • May 2018 – Present. The Food Waste Network (FoodWasteNet), a BBSRC Network in Industrial Biotechnology and Bioenergy (U.K. based).
  • April 2018 – Present. Vaccine Research Network (VRN) at Imperial College London.
  • March 2012 – Present. European Society of Cardiology (ESC).
  • February 2012 – Present. American Association for the Advancement of Science (AAAS).
  • November 2010 – Present. European Association for Cancer Research (EACR).

I am regularly invited to speak at scientific and industry conferences, and to advise policy experts, non-governmental organisations and companies. For example, I provided consultancy on techno-economic modelling to the WHO mRNA Technology Transfer Hub. I sit on the Sanofi mRNA CMC Advisory Board and the Pfizer mRNA Technology Advisory Board.