The Wolfson Light Microscopy Facility
The Light Microscopy Facility (LMF) is an open access facility of the Faculty of Science at the University of Sheffield. It offers a range of microscopes, including confocal (scanning and spinning disc), widefield and deconvolution microscopes. There are also three super-resolution light microscopes, one based on Structured Illumination, another on Localisation microscopy, an AiryScan confocal and a light sheet microscope. The microscopes are available to all members of the Faculty of Science. University users from outside the faculty with no access to the type of microscopy needed are welcome as are external user. Please get in touch with us to discuss your imaging needs and we will point you to the best system.
The Facility is located on B Floor in the North Wing of Firth Court and can be accessed by using the far right corner entrance when coming from the quad (the green central area of Firth Court).
- Darren Robinson, LMF Manager: email@example.com, 0114 2224682
- Christa Walther, Senior Experimental Officer in Super-resolution Light Microscopy: firstname.lastname@example.org, 0114 2222776
- Nick van Hateren, Light Sheet Microscope Manager: email@example.com, 0114 2222377
The diversity of systems available offers a range of techniques. We offer training in the use of the systems and advice for a number of techniques such as:
- Brightfield, DIC, phase contrast imaging
- Fluorescence imaging
- Live cell imaging
- Photoactivation/ Photobleaching (e.g. FRAP)
- TIRF microscopy
- Super-resolution Microscopy (SIM/STORM/PALM/ AiryScan)
- Light Sheet Microscopy
If you are interested in using microscopy for a project please get in touch and we will try to answer your questions, e.g. about which microscope to choose, appropriate sample preparation and relevant image analysis. For further information on the different systems, what they have been used for and how to access them, please use the links above.
Acknowledging the Wolfson Light Microscopy Facility
If you include any images obtained from the Wolfson Light Microscopy Facility within your published work please ensure that you acknowledge the Facility, stating for example: "Imaging work was performed at the Wolfson Light Microscopy Facility, using the [insert relevant type] microscope." Any publication using data acquired with either the N-STORM or the OMX the MRC grant MK/K0157531/1 needs to be acknowledged.
This will help to underpin the existence of the Wolfson Light Microscopy Facility by demonstrating its value to the research community and thereby enhance future funding applications.
On the page below you can find a list of recent publications containing data obtained within the Wolfson Light Microscopy Facility.
Please email us with the details of any publication acknowledging the facility and we will be happy to include your publication in the list above.
Multiphoton Microscopy is available at the Kroto Imaging Facility within the Centre for Biomaterials and Tissue Engineering. For more information please contact:
- Dr Nicola Green, firstname.lastname@example.org
Multiphoton Microscopy & intravital imaging is available at the Microscopy Core Facility in the Medical School. For more information please contact:
- Colin Gray, 0114 2261234, email@example.com
A Flow Cytometry core facilitiy exists in the Medical School. The facility provides a central service for experimental design and samples acquisition. For prices and information please contact:
- Medical School, 0114 2259084, firstname.lastname@example.org
High Content Imaging service is provided by the RNAi Facility (SRSF) within the Department of Biomedical Sciences. For information on design, carry out and interpretation of screening studies please contact:
- Dr Steve Brown, 0114 2223690, email@example.com
Cryo-Electron Microscopy is available within the Molecular Biology and Biotechnology department. Further information can be found here www.sheffield.ac.uk/mbb/research/facilities/cryo-em.