Professor Colin D Bingle
Professor of Respiratory Cell and Molecular Biology
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield
Beech Hill Road
Tel: +44 (0)114 215 9514
Fax: +44 (0)114 271 1863
Currently, I am a Professor of Respiratory Cell and Molecular Biology in the Department of Infection, Immunity and Cardiovascular Disease at the University of Sheffield. I have been at Sheffield since 1997 when I was appointed to a Lectureship to support the development of molecular and cell biology within the Unit following the appointment of Professor Moira Whyte.
My research has been focused on lung epithelial cell biology since I undertook a Postdoctoral Fellowship at Washington University in St Louis between 1991 and 1993. During that time I became interested in the molecular basis of cell type specific gene expression and I published the first ever paper that identified a specific functional role for a transcription factor (HNF-3) in the regulation of a lung specific gene (Biochem J 1993;295:227-232). Since this time my work has continued to employ molecular biology techniques to address questions relating to the regulation, structure, expression and function of pulmonary epithelial genes.
My long-standing research interests have been focused on cellular differentiation and regulation of gene expression within the developing and adult pulmonary epithelium. This work involves the isolation of pulmonary epithelial cell specific genes as well as the transcription factors, which regulate their expression. Over the past few years my interests have expanded into the fields of the genetics of complex diseases, pulmonary immunology, innate immunity and host defence. Over my time in Sheffield I have also come to appreciate the importance of utilising clinical material as a scientific resource and I have established multiple collaborations to enable the collection and use of such samples for my work.
My teaching interests lie in aspects of genetics and comparative genomics which I teach on the MSc in Molecular Medicine. I also teach on the Undergraduate Medical Course
- I am a member of the Council of the European Respiratory Society.
- I am a member of the Animal Science Group at the Society of Biology
- I am Faculty representative on the University Health and Safety Committee and Chair of the Medical School Health and Safety Committee.
- I am Editor of Biochemical Society Transactions.
- I am on the Editorial Board of Experimental Lung Research and I am an Editorial Advisor for the Biochemical Journal.
My research projects largely focus on the identification and characterisation of novel innate immune regulators, particularly members of the WFDC (Oncogene 2002;21:2768-2773) and PLUNC (Hum Mol Gen 2002;11:937-943) protein families. Indeed we identified the later family of proteins and they have gone on to become a significant area of my research focus. Laterly, I have become involved in studies designed to investigate the function and expression of these genes in the developing and diseased respiratory tract using a wide range of clinical samples (J Path 2005; 205: 491-497, Resp Res 2006; April 6;7:61, Resp Res 2007; Nov7:8:79).
My continuing and future studies in this area will elucidate the role these proteins play in a range of pulmonary disease including chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, cystic fibrosis (CF) and asthma in adults as well as in neonatal and paediatric diseases including RSV bronchiolitis and otitis media. I am also expanding my studies into the more tractable model systems with the aim of identifying the function of individual genes from these gene families.
I also work extensively with primary human airway epithelial cells in differentiated air liquid interface cultures as tools to understand the regulation of airway epithelial cell specific genes. At a more clinical level we are also studying host/pathogen interactions using both viral (RSV) and bacterial (Pseudomonas and NTHi) infections. We are presently using these models to understand the biology of co-infections with both RSV and NTHi.
I am using comparative genomic approaches to identify the molecular basis for the evolution of these host defence protein families. This work involves analysis and characterisation of gene families from a range of vertebrate species including chickens, cows and frogs as well as SNP analysis and studies of recent evolutionary events in the primate lineage.
2013 - 2016 £324,581 Biotechnology and Biological Sciences Research Council. The role of SPLUNC1/BPIFA1 in the host response to respiratory virus infection.
2013 - 2017 £61,000 University of Sheffield Florey Institute Research Studentship. Interactions of airway glycoproteins with Staphylococcus aureus in the respiratory epithelium
2012 - 2015 £52,000 University of Sheffield Research Studentship. The role of PLUNCs in otitis media.
2014 - 2018 £72,514 University of Sheffield A*STAR Research Studentship. Generation of Primary Ciliary Dyskinesia (PCD) phenotype in vitro and assessment of impaired ciliary function on pathogen infection (with Dr Sudipito Roy).
2014 - 2017 £54,114 University of Sheffield Research Studentship. The role of BPIFA1 in the respiratory epithelium
For key publications see below. For a full list of publications click here.
- Mulay A, Hood DW, Williams D, Russell C, Brown SDM, Bingle L, Cheeseman M & Bingle CD (2018) Loss of the homeostatic protein BPIFA1, leads to exacerbation of otitis media severity in the Junbo mouse model. Scientific Reports, 8(1). View this article in WRRO
- Akram K, Moyo N, Leeming G, Bingle L, Jasim S, Hussain S, Schorlemmer A, Kipar A, Digard P, Tripp R, Shohet V, Bingle C & Stewart J (2017) An innate defense peptide BPIFA1/SPLUNC1 restricts influenza A virus infection. Mucosal immunology, 11, 71-81. View this article in WRRO
- Mulay A, Akram K, Williams D, Armes H, Russell C, Hood D, Armstrong S, Stewart JP, Brown SD, Bingle L & Bingle CD (2016) An in vitro model of murine middle ear epithelium.. Disease Models and Mechanisms, 9, 1405-1417. View this article in WRRO
- Leeming GH, Kipar A, Hughes DJ, Bingle L, Bennett E, Moyo NA, Tripp RA, Bigley AL, Bingle CD, Sample JT & Stewart JP (2015) Gammaherpesvirus infection modulates the temporal and spatial expression of SCGB1A1 (CCSP) and BPIFA1 (SPLUNC1) in the respiratory tract. Laboratory Investigation, 95(6), 610-624. View this article in WRRO
- Gao J, Ohlmeier S, Nieminen P, Toljamo T, Tiitinen S, Kanerva T, Bingle L, Araujo B, Rönty M, Höyhtyä M, Bingle CD, Mazur W & Pulkkinen V (2015) Elevated sputum BPIFB1 levels in smokers with chronic obstructive pulmonary disease: a longitudinal study. American Journal of Physiology-Lung Cellular and Molecular Physiology, 309(1), L17-L26.
- Saferali A, Obeidat M, Bérubé J-C, Lamontagne M, Bossé Y, Laviolette M, Hao K, Nickle DC, Timens W, Sin DD, Postma DS, Strug LJ, Gallins PJ, Paré PD, Bingle CD & Sandford AJ (2015) Polymorphisms Associated with Expression of BPIFA1/BPIFB1 and Lung Disease Severity in Cystic Fibrosis. American Journal of Respiratory Cell and Molecular Biology, 53(5), 607-614.
- Bingle CD, Araujo B, Wallace WA, Hirani N & Bingle L (2013) What is top of the charts? BPIFB1/LPLUNC1 localises to the bronchiolised epithelium in the honeycomb cysts in UIP. Thorax, 68(12), 1167-1168.
- Musa M, Wilson K, Sun L, Mulay A, Bingle L, Marriott HM, LeClair EE & Bingle CD (2012) Differential localisation of BPIFA1 (SPLUNC1) and BPIFB1 (LPLUNC1) in the nasal and oral cavities of mice.. Cell Tissue Res, 350(3), 455-464. View this article in WRRO
- Bingle L, Wilson K, Musa M, Araujo B, Rassl D, Wallace WA, LeClair EE, Mauad T, Zhou Z, Mall MA & Bingle CD (2012) BPIFB1 (LPLUNC1) is upregulated in cystic fibrosis lung disease.. Histochem Cell Biol, 138(5), 749-758. View this article in WRRO
- Bingle CD (2011) Towards defining the complement of mammalian WFDC-domain-containing proteins.. Biochem Soc Trans, 39(5), 1393-1397.
- Shin OS, Uddin T, Citorik R, Wang JP, Della Pelle P, Kradin RL, Bingle CD, Bingle L, Camilli A, Bhuiyan TR, Shirin T, Ryan ET, Calderwood SB, Finberg RW, Qadri F, Larocque RC & Harris JB (2011) LPLUNC1 modulates innate immune responses to Vibrio cholerae.. J Infect Dis, 204(9), 1349-1357.
- Bingle CD, Seal RL & Craven CJ (2011) Systematic nomenclature for the PLUNC/PSP/BSP30/SMGB proteins as a subfamily of the BPI fold-containing superfamily.. Biochemical Society Transactions, 39(4), 977-983. View this article in WRRO
- Bingle CD, Wilson K, Lunn H, Barnes FA, High AS, Wallace WA, Rassl D, Campos MA, Ribeiro M & Bingle L (2010) Human LPLUNC1 is a secreted product of goblet cells and minor glands of the respiratory and upper aerodigestive tracts.. Histochem Cell Biol, 133(5), 505-515. View this article in WRRO
- Bingle L, Barnes FA, Lunn H, Musa M, Webster S, Douglas CWI, Cross SS, High AS & Bingle CD (2009) Characterisation and expression of SPLUNC2, the human orthologue of rodent parotid secretory protein.. Histochem Cell Biol, 132(3), 339-349. View this article in WRRO
- Bingle L, Barnes FA, Cross SS, Rassl D, Wallace WA, Campos MA & Bingle CD (2007) Differential epithelial expression of the putative innate immune molecule SPLUNC1 in cystic fibrosis.. Respir Res, 8, 79. View this article in WRRO
- Bingle L, Cross SS, High AS, Wallace WA, Rassl D, Yuan G, Hellstrom I, Campos MA & Bingle CD (2006) WFDC2 (HE4): a potential role in the innate immunity of the oral cavity and respiratory tract and the development of adenocarcinomas of the lung.. Respir Res, 7, 61. View this article in WRRO
- Bingle CD & Craven CJ (2002) PLUNC: a novel family of candidate host defence proteins expressed in the upper airways and nasopharynx.. Hum Mol Genet, 11(8), 937-943.