Professor Colin D Bingle
Professor of Respiratory Cell and Molecular Biology
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield
Beech Hill Road
Tel: +44 (0)114 215 9514
Fax: +44 (0)114 271 1863
I am Professor of Respiratory Cell and Molecular Biology in the Department of Infection, Immunity and Cardiovascular Disease at the University of Sheffield. I have been at Sheffield since 1997 when I was appointed to a Lectureship to support the development of molecular and cell biology within the Department of Medicine and Pharmacology.
My research has been focused on lung epithelial cell biology since I undertook a Postdoctoral Fellowship at Washington University in St Louis between 1991 and 1993. During that time I became interested in the molecular basis of cell type specific gene expression and I published the first ever paper that identified a specific functional role for a transcription factor (HNF-3α) in the regulation of a lung specific gene (Biochem J 1993;295:227-232). Since this time my work has continued to employ molecular biology techniques to address questions relating to the regulation, structure, expression and function of pulmonary epithelial genes.
My long-standing research interests have been focused on cellular differentiation and regulation of gene expression within the developing and adult pulmonary epithelium. This work involves the isolation of pulmonary epithelial cell specific genes as well as the transcription factors, which regulate their expression. Over the past few years my interests have expanded into the fields of the genetics of complex diseases, pulmonary immunology, innate immunity and host defence. During my time in Sheffield I have also come to appreciate the importance of utilising clinical material as a scientific resource and I have established multiple collaborations to undertake such work.
My teaching interests lie in aspects of genetics and comparative genomics, which I teach on the MSc in Molecular Medicine. I also teach on the Undergraduate Medical and Dental Courses.
- I am Chair of the Biochemical Society Awards Committee.
- I am a member of The Fellowship and Awards Committee of the European Respiratory Society.
- I am Editor-in-Chief of Biochemical Society Transactions.
- I am Faculty representative on the University Health and Safety Committee .
My research projects largely focus on the identification and characterisation of novel innate immune regulators, particularly members of the WFDC (Oncogene 2002;21:2768-2773) and BPIF/PLUNC (Hum Mol Gen 2002;11:937-943) protein families. Indeed we identified the later family of proteins and they have gone on to become a significant area of my research focus. Latterly, I have become involved in studies designed to investigate the function and expression of these genes in the developing and diseased respiratory tract using a wide range of clinical samples and understanding the biology of these genes is a key focus of my work. My continuing studies in this area are designed to elucidate the role these proteins play in a range of pulmonary disease including chronic obstructive pulmonary disease (COPD), pulmonary fibrosis, cystic fibrosis (CF) and asthma in adults as well as in neonatal and paediatric diseases including RSV bronchiolitis and otitis media. I have expanded my studies into the more tractable murine model systems with the aim of identifying the function of individual genes from these gene families.
My group works extensively with primary airway epithelial cells (both human and murine) in differentiated air liquid interface cultures as tools to understand the regulation of airway epithelial cell specific genes. This work also now involves studies of salivary gland biology. At a more clinical level we are also studying host/pathogen interactions using both viral (Influenza A, RSV) and bacterial (Staphylococcus aureus and NTHi) infections. We are presently developing these models to understand the biology of co-infections as well as the role of inflammatory cells
This applied work has allowed me to continue to study the role of epithelial differentiation in the respiratory tract and to use disease models and our novel genes to study aspects of pulmonary cell plasticity in the lung. It has also led to new avenues of work on epithelial responses in nasopharyngeal infections and in otitis media and latterly to more applied studies on the biology of multiciliogenesis.
2018 – 2019: Biotechnology and Biological Sciences Research Council project grant. BPIFA1: from anti-viral peptide to immunomodulator.
2017 – 2021: Commonwealth Scholarship PhD Studentship. Dissecting the genetics and proteomics of the airway epithelia to identify novel mediators of multiciliogenesis pathways.
2018 – 2021: Government of Saudi Arabia PhD Studentship. The role of host cells and Staphylococcus aureus virulence factors in the initiation of respiratory infection.
2018 – 2021: Government of Saudi Arabia PhD Studentship. A role for BPIFB1 in the immunity of the respiratory tract.
2017 – 2020: Government of Saudi Arabia PhD Studentship. Uncovering the role of BPIFA1 in Influenza infection of the lung.
For key publications see below. For a full list of publications click here.
- Lu H, Anujan P, Zhou F, Zhang Y, Chong YL, Bingle CD & Roy S (2019) Mcidas mutant mice reveal a two-step process for the specification and differentiation of multiciliated cells in mammals. Development, 146(6).
- Mulay A, Hood DW, Williams D, Russell C, Brown SDM, Bingle L, Cheeseman M & Bingle CD (2018) Loss of the homeostatic protein BPIFA1, leads to exacerbation of otitis media severity in the Junbo mouse model. Scientific Reports, 8(1). View this article in WRRO
- Akram K, Moyo N, Leeming G, Bingle L, Jasim S, Hussain S, Schorlemmer A, Kipar A, Digard P, Tripp R , Shohet V et al (2017) An innate defense peptide BPIFA1/SPLUNC1 restricts influenza A virus infection. Mucosal immunology, 11, 71-81. View this article in WRRO
- Mulay A, Akram K, Williams D, Armes H, Russell C, Hood D, Armstrong S, Stewart JP, Brown SD, Bingle L & Bingle CD (2016) An in vitro model of murine middle ear epithelium.. Disease Models and Mechanisms, 9, 1405-1417. View this article in WRRO
- Leeming GH, Kipar A, Hughes DJ, Bingle L, Bennett E, Moyo NA, Tripp RA, Bigley AL, Bingle CD, Sample JT & Stewart JP (2015) Gammaherpesvirus infection modulates the temporal and spatial expression of SCGB1A1 (CCSP) and BPIFA1 (SPLUNC1) in the respiratory tract. Laboratory Investigation, 95(6), 610-624. View this article in WRRO
- Gao J, Ohlmeier S, Nieminen P, Toljamo T, Tiitinen S, Kanerva T, Bingle L, Araujo B, Rönty M, Höyhtyä M , Bingle CD et al (2015) Elevated sputum BPIFB1 levels in smokers with chronic obstructive pulmonary disease: a longitudinal study. American Journal of Physiology-Lung Cellular and Molecular Physiology, 309(1), l17-l26.
- Saferali A, Obeidat ME, Bérubé J-C, Lamontagne M, Bossé Y, Laviolette M, Hao K, Nickle DC, Timens W, Sin DD , Postma DS et al (2015) Polymorphisms Associated with Expression of BPIFA1/BPIFB1 and Lung Disease Severity in Cystic Fibrosis. American Journal of Respiratory Cell and Molecular Biology, 53(5), 607-614.
- Bingle CD, Araujo B, Wallace WA, Hirani N & Bingle L (2013) What is top of the charts? BPIFB1/LPLUNC1 localises to the bronchiolised epithelium in the honeycomb cysts in UIP. Thorax, 68(12), 1167-1168.
- Musa M, Wilson K, Sun L, Mulay A, Bingle L, Marriott HM, LeClair EE & Bingle CD (2012) Differential localisation of BPIFA1 (SPLUNC1) and BPIFB1 (LPLUNC1) in the nasal and oral cavities of mice.. Cell Tissue Res, 350(3), 455-464. View this article in WRRO
- Bingle L, Wilson K, Musa M, Araujo B, Rassl D, Wallace WA, LeClair EE, Mauad T, Zhou Z, Mall MA & Bingle CD (2012) BPIFB1 (LPLUNC1) is upregulated in cystic fibrosis lung disease.. Histochem Cell Biol, 138(5), 749-758. View this article in WRRO
- Bingle CD (2011) Towards defining the complement of mammalian WFDC-domain-containing proteins.. Biochem Soc Trans, 39(5), 1393-1397.
- Shin OS, Uddin T, Citorik R, Wang JP, Della Pelle P, Kradin RL, Bingle CD, Bingle L, Camilli A, Bhuiyan TR , Shirin T et al (2011) LPLUNC1 modulates innate immune responses to Vibrio cholerae.. J Infect Dis, 204(9), 1349-1357.
- Bingle CD, Seal RL & Craven CJ (2011) Systematic nomenclature for the PLUNC/PSP/BSP30/SMGB proteins as a subfamily of the BPI fold-containing superfamily.. Biochemical Society Transactions, 39(4), 977-983. View this article in WRRO
- Bingle CD, Wilson K, Lunn H, Barnes FA, High AS, Wallace WA, Rassl D, Campos MA, Ribeiro M & Bingle L (2010) Human LPLUNC1 is a secreted product of goblet cells and minor glands of the respiratory and upper aerodigestive tracts.. Histochem Cell Biol, 133(5), 505-515. View this article in WRRO
- Bingle L, Barnes FA, Lunn H, Musa M, Webster S, Douglas CWI, Cross SS, High AS & Bingle CD (2009) Characterisation and expression of SPLUNC2, the human orthologue of rodent parotid secretory protein.. Histochem Cell Biol, 132(3), 339-349. View this article in WRRO
- Bingle L, Barnes FA, Cross SS, Rassl D, Wallace WA, Campos MA & Bingle CD (2007) Differential epithelial expression of the putative innate immune molecule SPLUNC1 in cystic fibrosis.. Respir Res, 8, 79. View this article in WRRO
- Bingle L, Cross SS, High AS, Wallace WA, Rassl D, Yuan G, Hellstrom I, Campos MA & Bingle CD (2006) WFDC2 (HE4): a potential role in the innate immunity of the oral cavity and respiratory tract and the development of adenocarcinomas of the lung.. Respir Res, 7, 61. View this article in WRRO
- Bingle CD & Craven CJ (2002) PLUNC: a novel family of candidate host defence proteins expressed in the upper airways and nasopharynx.. Hum Mol Genet, 11(8), 937-943.