Theory & Practise

Beginners ImmunohistochemistryBeginners Immunohistochemistry

7-8 March 2018 – book now

This course is accredited by the Institute of Biomedical Science

This course will provide a basic training in IHC theory and practise to those working within the NHS, higher education institutions or within industry. The course will teach you the knowledge and confidence to manipulate basic IHC techniques to fit applications within your workplace.

On completion of the course, students will understand:

  • The complex interactions of an antigen and antibody and how this impacts on immunohistochemistry reactions
  • Mathematics relating to molarity and ratios

On completion of the course, students will be able to:

  • Apply a sound theoretical approach to the application of immunohistochemistry within the work environment
  • Carry out immunohistochemistry protocols to demonstrate how proteins are distributed within a tissue section
  • Identify positive staining within a tissue section using a light microscope
  • Discuss the interaction of an antigen and antibody and how this impacts on the immunohistochemistry reactions
  • Explain why the use of control samples are important.
  • Prepare reagents and antibodies including calculation of molarity and dilutions
  • Use histology laboratory equipment safely
  • Complete a risk assessment for a piece of equipment and input a chemical onto a COSHH form

The topics covered in the module include:

  • IHCIntroduction to IHC
  • History and background of IHC staining
  • Laboratory safety including COSHH and risk assessments
  • Fixation techniques, antigen retrieval
  • Mathematics for IHC
  • Tissue sectioning, non-specific staining
  • Control samples
  • False positive and false negative staining
  • Wax processing
  • IHC staining techniques
  • Light microscopy

Is the course right for me?

The beginners IHC course is suitable for anyone who has a basic science background and wishes to gain an understanding of theory and practical experience in current and popular immunohistochemical techniques. It will consist of a series of lectures and lab based practical sessions and is taught over two days. Refreshment breaks and lunch will be provided.

The course will provide you with a sound knowledge of the basics behind IHC and you will leave having gained the skills to perform and manipulate IHC on tissue to identify one antigen/cellular component in a single tissue section.

7-8 March 2018 – book now

SITraN, Department of Neuroscience, The University of Sheffield, Sheffied, S10 2HQ, United Kingdom

The cost of this two day course is £495. To find out more information, contact:

Natalie Kennerley -, 0114 222 6600
Lynne Baxter –, 0114 2222272/51