Spinning disk confocal imaging

Spinning disk confocal imaging is a high-speed fluorescence imaging technique optimised for capturing dynamic and light-sensitive processes in living cells. Its ability to rapidly acquire images across multiple focal planes makes it well-suited for studying fast intracellular events in real time.

In our research, we use spinning disk confocal imaging to visualise and track changes in intracellular calcium levels within cells of the auditory system under various experimental conditions. This is particularly useful because calcium signaling plays a critical role in auditory cell function, including neurotransmission and cellular excitability. By monitoring these changes, we gain valuable insights into how auditory cells respond to stimuli, stress, or injury which contributes to our understanding of the mechanisms underlying hearing function and dysfunction.