Professor Stephen A Renshaw
MA FRCP PhD
School of Medicine and Population Health
Head of Division of Clinical Medicine
Deputy Dean of School of Medicine and Population Health
Sir Arthur Hall Professor of Medicine
s.a.renshaw@sheffield.ac.uk
+44 114 215 9574
+44 114 215 9574
+44 114 215 9004
(Lindsey Crowson - PA)
Room FU33, F Floor, The Medical School
Full contact details
Professor Stephen A Renshaw
School of Medicine and Population Health
Room FU33, F Floor
The Medical School
Beech Hill Road
Sheffield
S10 2RX
School of Medicine and Population Health
Room FU33, F Floor
The Medical School
Beech Hill Road
Sheffield
S10 2RX
- Profile
-
For enquiries please contact - SMPH-West-Operational@sheffield.ac.uk
I have worked for the University of Sheffield since 1998. I am the Sir Arthur Hall Professor of Medicine and Director of the Bateson Centre - a large cross-faculty research centre (former MRC Centre, 40PIs, over 130 members and active grant funding of over £30m).
I have held important leadership roles in large cross-institution partnerships, including being founding director of an MRC Doctoral Training Partnership across 4 major civic universities of the North of England – highly regarded as a model for student-led, cross-institutional, innovative postgraduate research training. I also have national and International leadership roles through membership of REF 2021 sub-panel 1, and having been principal organiser of Neutrophil 2021 and the Zebrafish Disease Models meeting 2022.
I studied medicine at Cambridge and then at Oxford Clinical School. After a clinical rotation in Nottingham, I moved to Sheffield to take up a Wellcome Trust Clinical Research Training Fellowship, to work on the regulation of neutrophil lifespan with Professor Moira Whyte.
After 3 years as Clinical Lecturer in Respiratory Medicine, I was awarded an MRC Clinician Scientist Fellowship to develop an innovative model of neutrophil biology in the transparent, genetically-tractable larval zebrafish. I have been able to develop a number of unique transgenic zebrafish which have allowed several important advances in our understanding of inflammation biology and of host-pathogen interaction.
In 2008 I was awarded an MRC Senior Clinical Fellowship and in 2014 an MRC Programme Grant to continue this work. I continue clinical work in Respiratory Medicine with a special interest in Interstitial Lung Disease associated with a range of multisystem diseases.
- Research interests
-
Diseases of immunity cause much illness in the developed world – on one hand we are beset by a range of antibiotic resistance bacterial infections, while on the other hand our immune systems are responsible for many the common diseases of ageing – heart disease, stroke and COPD.
Understanding the regulation of innate immune cells, neutrophils and macrophages, in infection and inflammation will help us tune the immune system to the exact level needed to cope with the current level of threat. More host defence to fight antibiotic-resistant organisms; less host defence to prevent lung damage in response to environmental pollutants.
To improve our understanding, I have set up a model system in which the genes controlling regulation of innate immune cell function can be identified. The model I have chosen is the Zebrafish, which is both genetically manipulable and transparent, leading to easy visualisation of immune cells during infection and inflammation.
This model allows me to test the ability of a range of candidate genes to influence host-pathogen interaction and the resolution of inflammation, and additionally to screen for novel genes involved in this process. At the same time, I can see every immune cell during the whole of an infection or an inflammatory episode, where necessary imaging intracellular signalling events in real-time.
The small size of our model also lends itself to drug screening and this has identified several potential new therapies for immune disease.
Current projects
- Identification of key regulatory pathways in inflammation resolution.
- Non-mammalian models of host-pathogen interaction.
- Identification of novel compounds targeting inflammation resolution using zebrafish in vivo screens.
- Publications
-
Show: Featured publications All publications
Featured publications
Journal articles
- Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection. PLoS Pathogens, 18(4). View this article in WRRO
- Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics. Proceedings of the National Academy of Sciences, 118(44). View this article in WRRO
- Commensal bacteria augment Staphylococcus aureus infection by inactivation of phagocyte-derived reactive oxygen species. PLoS Pathogens, 17(9).
- Pioneer neutrophils release chromatin within in vivo swarms. eLife, 10. View this article in WRRO
- Neutrophils use selective autophagy receptor Sqstm1/p62 to target Staphylococcus aureus for degradation in vivo in zebrafish. Autophagy, 17(6), 1448-1457. View this article in WRRO
- Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction. PLoS Pathogens, 17(3). View this article in WRRO
- Human-specific staphylococcal virulence factors enhance pathogenicity in a humanised zebrafish C5a receptor model. Journal of Cell Science, 134(5).
- Deletion of cftr leads to an excessive neutrophilic response and defective tissue repair in a zebrafish model of sterile inflammation. Frontiers in Immunology, 11(1).
- Polymersomes eradicating intracellular bacteria. ACS Nano, 14(7), 8287-8298. View this article in WRRO
- The failure of microglia to digest developmental apoptotic cells contributes to the pathology of RNASET2-deficient leukoencephalopathy. Glia, 68(7), 1531-1545. View this article in WRRO
- Semaphorin 3F signaling actively retains neutrophils at sites of inflammation. Journal of Clinical Investigation, 130(6), 3221-3237. View this article in WRRO
- The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils. Autophagy, 17(4), 888-902.
- Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation. eLife, 8. View this article in WRRO
- The CXCL12/CXCR4 signaling axis retains neutrophils at inflammatory sites in zebrafish. Frontiers in Immunology, 10. View this article in WRRO
- 15-keto-prostaglandin E2 activates host peroxisome proliferator-activated receptor gamma (PPAR-γ) to promote Cryptococcus neoformans growth during infection. PLoS Pathogens, 15(3). View this article in WRRO
- CFTR protects against Mycobacterium abscessus infection by fine-tuning host oxidative defenses. Cell Reports, 26(7), 1828-1840.e4. View this article in WRRO
- PGE2 production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolution in vivo. Science Advances, 4(9). View this article in WRRO
- Human skin commensals augment Staphylococcus aureus pathogenesis. Nature Microbiology, 3(8), 881-890. View this article in WRRO
- Reverse Migration of Neutrophils: Where, When, How, and Why?. Trends in Immunology, 37(5), 273-286.
- A Spaetzle-like role for nerve growth factor beta in vertebrate immunity to Staphylococcus aureus. SCIENCE, 346(6209), 641-646. View this article in WRRO
- Tnfa signaling through tnfr2 protects skin against oxidative stress-induced inflammation.. PLoS Biol, 12(5), e1001855.
- The IL-1 family in fish: swimming through the muddy waters of inflammasome evolution.. Dev Comp Immunol, 46(1), 53-62.
- Clonal expansion during Staphylococcus aureus infection dynamics reveals the effect of antibiotic intervention.. PLoS Pathog, 10(2), e1003959.
- A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism.. Sci Transl Med, 6(225), 225ra29.
- Serum and glucocorticoid-regulated kinase 1 regulates neutrophil clearance during inflammation resolution.. J Immunol, 192(4), 1796-1805.
- Zebrafish tissue injury causes upregulation of interleukin-1 and caspase-dependent amplification of the inflammatory response.. Dis Model Mech, 7(2), 259-264.
- Hypoxia inducible factor signaling modulates susceptibility to mycobacterial infection via a nitric oxide dependent mechanism.. PLoS Pathog, 9(12), e1003789.
- Zebrafish as a model for the study of neutrophil biology.. J Leukoc Biol, 94(4), 633-642.
- Repelled from the wound, or randomly dispersed? Reverse migration behaviour of neutrophils characterized by dynamic modelling.. J R Soc Interface, 9(77), 3229-3239.
- Neutrophil-delivered myeloperoxidase dampens the hydrogen peroxide burst after tissue wounding in zebrafish. Current Biology, 22(19), 1818-1824.
- A privileged intraphagocyte niche is responsible for disseminated infection of Staphylococcus aureus in a zebrafish model.. Cell Microbiol, 14(10), 1600-1619.
- Live Imaging of Tumor Initiation in Zebrafish Larvae Reveals a Trophic Role for Leukocyte-Derived PGE 2. Current Biology.
- Deficiency of tumour necrosis factor-related apoptosis-inducing ligand exacerbates lung injury and fibrosis.. Thorax, 67(9), 796-803.
- A model 450 million years in the making: zebrafish and vertebrate immunity.. Dis Model Mech, 5(1), 38-47.
- Simultaneous intravital imaging of macrophage and neutrophil behaviour during inflammation using a novel transgenic zebrafish.. Thromb Haemost, 105(5), 811-819.
- Activation of hypoxia-inducible factor-1α (Hif-1α) delays inflammation resolution by reducing neutrophil apoptosis and reverse migration in a zebrafish inflammation model.. Blood, 118(3), 712-722.
- Pivotal Advance: Pharmacological manipulation of inflammation resolution during spontaneously resolving tissue neutrophilia in the zebrafish.. J Leukoc Biol, 87(2), 203-212.
- A novel vertebrate model of Staphylococcus aureus infection reveals phagocyte-dependent resistance of zebrafish to non-host specialized pathogens.. Cell Microbiol, 10(11), 2312-2325.
- Identifying and hurdling obstacles to translational research.. Nat Rev Immunol, 7(1), 77-82.
- A transgenic zebrafish model of neutrophilic inflammation.. Blood, 108(13), 3976-3978.
- Acceleration of human neutrophil apoptosis by TRAIL.. J Immunol, 170(2), 1027-1033.
All publications
Journal articles
- Correction: A subset of neutrophil phagosomes is characterised by pulses of Class I PI3K activity. Disease Models & Mechanisms, 18(10).
- Determining the importance of the stringent response for methicillin-resistant staphylococcus aureus virulence in vivo. The Journal of Infectious Diseases. View this article in WRRO
- A subset of neutrophil phagosomes is characterised by pulses of Class I PI3K activity. Disease Models & Mechanisms, 18(9). View this article in WRRO
- EPS7.09Verapamil limits inflammatory damage by reducing epithelial calcium activity in preclinical models of cystic fibrosis. Journal of Cystic Fibrosis, 24, S62-S62.
- Curcumin-mediated NRF2 induction limits inflammatory damage in, preclinical models of cystic fibrosis. Biomedicine & Pharmacotherapy, 186. View this article in WRRO
- Tetracycline and oxacillin act synergistically on biofilms and display increased efficacy in vivo against staphylococcus aureus. Current Microbiology, 81. View this article in WRRO
- WS16.04: When cystic fibrosis triggers a protective immunity against tuberculosis: loss of CFTR confers resistance to Mycobacterium marinum infections in cystic fibrosis zebrafish models. Journal of Cystic Fibrosis, 23(Supp 1), S30-S30. View this article in WRRO
- P207 The longitudinal assessment of anti-virulence gene expression in Pseudomonas aeruginosa grown in cystic fibrosis sputum mimics. Journal of Cystic Fibrosis, 23, S132-S133.
- Macrophage transplantation rescues RNASET2-deficient leukodystrophy by replacing deficient microglia in a zebrafish model. Proceedings of the National Academy of Sciences, 121(21). View this article in WRRO
- Experimental evolution of Staphylococcus aureus in macrophages: dissection of a conditional adaptive trait promoting intracellular survival. mBio, 15(6). View this article in WRRO
- What can we learn about fish neutrophil and macrophage response to immune challenge from studies in zebrafish. Fish & Shellfish Immunology, 148. View this article in WRRO
- Dimethyl fumarate in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial. Nature Communications, 15. View this article in WRRO
- Correction to “A
p21‐GFP zebrafish model of senescence for rapid testing of senolytics in vivo”. Aging Cell, 23(3).
- Emerging strategies to target virulence in Pseudomonas aeruginosa respiratory infections. Critical Reviews in Microbiology, 50(6), 1037-1052. View this article in WRRO
- Analysis of the distal urinary tract in larval and adult zebrafish reveals homology to the human system. Disease Models & Mechanisms, 16(7). View this article in WRRO
- P235 Pharmacological activation of NRF2 has protective effects during Mycobacterium abscessus infection by promoting host defences and reducing inflammatory damage in the context of cystic fibrosis. Journal of Cystic Fibrosis, 22, S137-S137.
- Higher dose corticosteroids in patients admitted to hospital with COVID-19 who are hypoxic but not requiring ventilatory support (RECOVERY): a randomised, controlled, open-label, platform trial. The Lancet, 401(10387), 1499-1507. View this article in WRRO
- A p21‐GFP zebrafish model of senescence for rapid testing of senolytics in vivo. Aging Cell, 22(6). View this article in WRRO
- Activated PI3K Delta Syndrome-1 mutations cause neutrophilia in zebrafish larvae. Disease Models & Mechanisms, 16(3). View this article in WRRO
- Clonal population expansion of Staphylococcus aureus occurs due to escape from a finite number of intraphagocyte niches. Scientific Reports, 13(1), 1-11. View this article in WRRO
- Baricitinib in patients admitted to hospital with COVID-19 (RECOVERY) : a randomised, controlled, open-label, platform trial and updated meta-analysis. The Lancet, 400(10349), 359-368. View this article in WRRO
- The EGFR/ErbB inhibitor neratinib modifies the neutrophil phosphoproteome and promotes apoptosis and clearance by airway macrophages. Frontiers in Immunology, 13. View this article in WRRO
- A subset of gut leukocytes has telomerase-dependent “hyper-long” telomeres and require telomerase for function in zebrafish. Immunity & Ageing, 19(1).
- Phagosomal acidification Is required to kill Streptococcus pneumoniae in a Zebrafish model. Cellular Microbiology, 2022. View this article in WRRO
- Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection. PLoS Pathogens, 18(4). View this article in WRRO
- A zebrafish reporter line reveals immune and neuronal expression of endogenous retrovirus. Disease Models & Mechanisms, 15(4).
- Roscovitine Worsens Mycobacterium abscessus Infection by Reducing DUOX2-mediated Neutrophil Response. American Journal of Respiratory Cell and Molecular Biology, 66(4), 439-451.
- Casirivimab and imdevimab in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial. The Lancet, 399(10325), 665-676. View this article in WRRO
- Aspirin in patients admitted to hospital with COVID-19 (RECOVERY) : a randomised, controlled, open-label, platform trial. The Lancet, 399(10320), 143-151. View this article in WRRO
- Demonstration of the role of cell wall homeostasis in Staphylococcus aureus growth and the action of bactericidal antibiotics. Proceedings of the National Academy of Sciences, 118(44). View this article in WRRO
- Commensal bacteria augment Staphylococcus aureus infection by inactivation of phagocyte-derived reactive oxygen species. PLoS Pathogens, 17(9).
- Pioneer neutrophils release chromatin within in vivo swarms. eLife, 10. View this article in WRRO
- Neutrophils use selective autophagy receptor Sqstm1/p62 to target Staphylococcus aureus for degradation in vivo in zebrafish. Autophagy, 17(6), 1448-1457. View this article in WRRO
- Convalescent plasma in patients admitted to hospital with COVID-19 (RECOVERY): a randomised controlled, open-label, platform trial. The Lancet, 397(10289), 2049-2059. View this article in WRRO
- Tocilizumab in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial. The Lancet, 397(10285), 1637-1645. View this article in WRRO
- Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction. PLoS Pathogens, 17(3). View this article in WRRO
- Human-specific staphylococcal virulence factors enhance pathogenicity in a humanised zebrafish C5a receptor model. Journal of Cell Science, 134(5).
- Azithromycin in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial. Lancet, 397(10274), 605-612. View this article in WRRO
- The role of macrophages in Staphylococcus aureus infection. Frontiers in Immunology, 11. View this article in WRRO
- Quantification of pulmonary perfusion in idiopathic pulmonary fibrosis with first pass dynamic contrast-enhanced perfusion MRI. Thorax, 76(2), 144-151. View this article in WRRO
- Evaluation of the anti-inflammatory effects of synthesised tanshinone I and isotanshinone I analogues in zebrafish. PLOS ONE, 15(10). View this article in WRRO
- Deletion of cftr leads to an excessive neutrophilic response and defective tissue repair in a zebrafish model of sterile inflammation. Frontiers in Immunology, 11(1).
- Polymersomes eradicating intracellular bacteria. ACS Nano, 14(7), 8287-8298. View this article in WRRO
- The failure of microglia to digest developmental apoptotic cells contributes to the pathology of RNASET2-deficient leukoencephalopathy. Glia, 68(7), 1531-1545. View this article in WRRO
- Real world experience of response to pirfenidone in patients with idiopathic pulmonary fibrosis: a two centre retrospective study. Sarcoidosis Vasculitis and Diffuse Lung Diseases, 37(2), 218-224.
- Developing novel host-based therapies targeting microbicidal responses in macrophages and neutrophils to combat bacterial antimicrobial resistance. Frontiers in Immunology, 11.
- Semaphorin 3F signaling actively retains neutrophils at sites of inflammation. Journal of Clinical Investigation, 130(6), 3221-3237. View this article in WRRO
- The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils. Autophagy, 17(4), 888-902.
- Human-specific staphylococcal virulence factors enhance pathogenicity in a humanised zebrafish C5a receptor model.
- Identifying causation in hypersensitivity pneumonitis: a British perspective.. BMJ Open Respir Res, 6(1).
- 2601. Identification of Staphylococcus aureus Genetic Factors Associatiated with the Subversion of Macrophage Phagosomal Acidification. Open Forum Infectious Diseases, 6(Supplement_2), S904-S904.
- Polymeric nanobiotics as a novel treatment for mycobacterial infections. Journal of Controlled Release, 314, 116-124.
- Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation. eLife, 8. View this article in WRRO
- Evaluation of the anti-inflammatory effects of synthesised tanshinone I and isotanshinone I analogues in zebrafish.
- The CXCL12/CXCR4 signaling axis retains neutrophils at inflammatory sites in zebrafish. Frontiers in Immunology, 10. View this article in WRRO
- A transgenic zebrafish line for in vivo visualisation of neutrophil myeloperoxidase. PLoS ONE, 14(4). View this article in WRRO
- Neutrophils use selective autophagy receptor p62/SQSTM1 to targetStaphylococcus aureusfor degradationin vivoin zebrafish.
- Hyperpolarised xenon magnetic resonance spectroscopy for the longitudinal assessment of changes in gas diffusion in IPF. Thorax, 74(5), 500-502. View this article in WRRO
- 15-keto-prostaglandin E2 activates host peroxisome proliferator-activated receptor gamma (PPAR-γ) to promote Cryptococcus neoformans growth during infection. PLoS Pathogens, 15(3). View this article in WRRO
- The autophagic response toStaphylococcus aureusprovides an intracellular niche in neutrophils.
- CFTR protects against Mycobacterium abscessus infection by fine-tuning host oxidative defenses. Cell Reports, 26(7), 1828-1840.e4. View this article in WRRO
- Hif-1α-Induced Expression of Il-1β Protects against Mycobacterial Infection in Zebrafish.. Journal of Immunology, 202(2), 494-502. View this article in WRRO
- Staphylococcus aureus: setting its sights on the human innate immune system. Microbiology, 165(4), 367-385.
- PGE2 production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolution in vivo. Science Advances, 4(9). View this article in WRRO
- Human skin commensals augment Staphylococcus aureus pathogenesis. Nature Microbiology, 3(8), 881-890. View this article in WRRO
- A method for transplantation of human HSCs into zebrafish, to replace humanised murine transplantation models. F1000Research, 7(594). View this article in WRRO
- Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics. Methods Mol Biol, 1736, 147-156.
- The triune of intestinal microbiome, genetics and inflammatory status and its impact on lower gastrointestinal anastomosis healing. FEBS Journal, 285(7), 1212-1225. View this article in WRRO
- The identification of Staphylococcus aureus factors required for pathogenicity and growth in human blood. Infection and Immunity, 85(11). View this article in WRRO
- Dissemination ofCryptococcus neoformansvia localised proliferation and blockage of blood vessels.
- NR4A orphan nuclear receptor family members, NR4A2 and NR4A3, regulate neutrophil number and survival.. Blood, 130(8), 1014-1025. View this article in WRRO
- Bacterial size matters: multiple mechanisms controlling septum cleavage and diplococcus formation are critical for the virulence of the opportunistic pathogen Enterococcus faecalis. PLoS Pathogens, 13(7). View this article in WRRO
- Expression and regulation of drug transporters in vertebrate neutrophils. Scientific Reports, 7(4967). View this article in WRRO
- Targeting Neutrophilic Inflammation using Polymersome-Mediated Cellular Delivery. Journal of Immunology, 189(9), 3596-3604. View this article in WRRO
- Zebrafish screens for new colitis treatments - a bottom-up approach. The FEBS Journal, 284(3), 399-401.
- Purification of Nanoparticles by Size and Shape. Scientific Reports, 6. View this article in WRRO
- Identification of benzopyrone as a common structural feature in compounds with anti-inflammatory activity in a zebrafish phenotypic screen.. Disease Models and Mechanisms, 9(6), 621-632. View this article in WRRO
- Reverse Migration of Neutrophils: Where, When, How, and Why?. Trends in Immunology, 37(5), 273-286.
- Cryptococcus neoformans Intracellular Proliferation and Capsule Size Determines Early Macrophage Control of Infection.. Scientific Reports, 6. View this article in WRRO
- Defining the phenotype of neutrophils following reverse migration in zebrafish. Journal of Leukocyte Biology, 98(6), 975-981.
- Glucocerebrosidase 1 deficient Danio rerio mirror key pathological aspects of human Gaucher disease and provide evidence of early microglial activation preceding alpha-synuclein-independent neuronal cell death. Human Molecular Genetics, 24(23), 6640-6652. View this article in WRRO
- Exploring the HIFs, buts and maybes of hypoxia signalling in disease: lessons from zebrafish models. Disease Models & Mechanisms, 8(11), 1349-1360.
- Existence of a Colonizing Staphylococcus aureus Strain Isolated in Diabetic Foot Ulcers. Diabetes, 64(8), 2991-2995. View this article in WRRO
- Functional drug screening reveals anticonvulsants as enhancers of mTOR-independent autophagic killing of Mycobacterium tuberculosis through inositol depletion. EMBO Molecular Medicine, 7(2), 127-139. View this article in WRRO
- A Spaetzle-like role for nerve growth factor beta in vertebrate immunity to Staphylococcus aureus. SCIENCE, 346(6209), 641-646. View this article in WRRO
- PH-sensitive tubular polymersomes: Formation and applications in cellular delivery. ACS Nano, 8(5), 4650-4661.
- Tnfa signaling through tnfr2 protects skin against oxidative stress-induced inflammation.. PLoS Biol, 12(5), e1001855.
- The IL-1 family in fish: swimming through the muddy waters of inflammasome evolution.. Dev Comp Immunol, 46(1), 53-62.
- Clonal expansion during Staphylococcus aureus infection dynamics reveals the effect of antibiotic intervention.. PLoS Pathog, 10(2), e1003959.
- A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism.. Sci Transl Med, 6(225), 225ra29.
- Serum and glucocorticoid-regulated kinase 1 regulates neutrophil clearance during inflammation resolution.. J Immunol, 192(4), 1796-1805.
- Zebrafish tissue injury causes upregulation of interleukin-1 and caspase-dependent amplification of the inflammatory response.. Dis Model Mech, 7(2), 259-264.
- Inhibitors of neutrophil recruitment identified using transgenic zebrafish to screen a natural product library.. Dis Model Mech, 7(1), 163-169.
- Investigating the role of a prototype GIMAP in neutrophil regulation using an in vivo zebrafish model. IMMUNOLOGY, 143, 113-114.
- Hypoxic signalling modulates neutrophil nitric oxide in a zebrafish tuberculosis model. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 44, 12-12.
- Hypoxia inducible factor signaling modulates susceptibility to mycobacterial infection via a nitric oxide dependent mechanism.. PLoS Pathog, 9(12), e1003789.
- Zebrafish as a model for the study of neutrophil biology.. J Leukoc Biol, 94(4), 633-642.
- Zebrafish as a novel vertebrate model to dissect enterococcal pathogenesis.. Infect Immun, 81(11), 4271-4279.
- PhagoSight: an open-source MATLAB® package for the analysis of fluorescent neutrophil and macrophage migration in a zebrafish model.. PLoS One, 8(8), e72636.
- Cxcl8 (IL-8) Mediates Neutrophil Recruitment and Behavior in the Zebrafish Inflammatory Response. JOURNAL OF IMMUNOLOGY, 190(8), 4349-4359.
- Incremental shuttle walk test in the assessment of patients with obstructive sleep apnea-hypopnea syndrome.. J Sleep Res, 22(4), 471-477.
- Glucose metabolism impacts the spatiotemporal onset and magnitude of HSC induction in vivo. BLOOD, 121(13), 2483-2493.
- Hypoxia-inducible factor 2α regulates key neutrophil functions in humans, mice and zebrafish. Blood, 123(3), 366-376.
- Fully synthetic polymer vesicles for intracellular delivery of antibodies in live cells.. FASEB J, 27(1), 98-108.
- Repelled from the wound, or randomly dispersed? Reverse migration behaviour of neutrophils characterized by dynamic modelling.. J R Soc Interface, 9(77), 3229-3239.
- Neutrophil-delivered myeloperoxidase dampens the hydrogen peroxide burst after tissue wounding in zebrafish. Current Biology, 22(19), 1818-1824.
- A zebrafish model to study and therapeutically manipulate hypoxia signaling in tumorigenesis.. Cancer Res, 72(16), 4017-4027.
- Drift-Diffusion Analysis of Neutrophil Migration during Inflammation Resolution in a Zebrafish Model.. Adv Hematol, 2012, 792163.
- A privileged intraphagocyte niche is responsible for disseminated infection of Staphylococcus aureus in a zebrafish model.. Cell Microbiol, 14(10), 1600-1619.
- Live Imaging of Tumor Initiation in Zebrafish Larvae Reveals a Trophic Role for Leukocyte-Derived PGE 2. Current Biology.
- The neutrophil's eye-view: Inference and visualisation of the chemoattractant field driving cell chemotaxis in vivo. Plos One, 7(4).
- Deficiency of tumour necrosis factor-related apoptosis-inducing ligand exacerbates lung injury and fibrosis.. Thorax, 67(9), 796-803.
- A method for the in vivo measurement of zebrafish tissue neutrophil lifespan.. ISRN Hematol, 2012, 915868.
- A model 450 million years in the making: zebrafish and vertebrate immunity.. Dis Model Mech, 5(1), 38-47.
- Simultaneous intravital imaging of macrophage and neutrophil behaviour during inflammation using a novel transgenic zebrafish.. Thromb Haemost, 105(5), 811-819.
- The NOTCH pathway contributes to cell fate decision in myelopoiesis. Haematologica, 96(12), 1753-1760.
- Strategies of professional phagocytes in vivo: unlike macrophages, neutrophils engulf only surface-associated microbes. J CELL SCI, 124(18), 3053-3059.
- TNF-related apoptosis-inducing ligand (TRAIL) regulates inflammatory neutrophil apoptosis and enhances resolution of inflammation.. J Leukoc Biol, 90(5), 855-865.
- Activation of hypoxia-inducible factor-1α (Hif-1α) delays inflammation resolution by reducing neutrophil apoptosis and reverse migration in a zebrafish inflammation model.. Blood, 118(3), 712-722.
- A Zebrafish Model Of Delayed Inflammation Resolution: Pharmacological And Genetic Activation Of The HIF1a Pathway. B36. AIRWAY IMMUNE MECHANISMS AND INFLAMMATION: ANIMAL MODELS, A2858-A2858.
- Regulation of neutrophil senescence by microRNAs.. PLoS One, 6(1), e15810.
- Effective caspase inhibition blocks neutrophil apoptosis and reveals Mcl-1 as both a regulator and a target of neutrophil caspase activation.. PLoS One, 6(1), e15768.
- Zebrafish models of the immune response: taking it on the ChIn.. BMC Biol, 8, 148.
- Burkholderia cenocepacia Creates an Intramacrophage Replication Niche in Zebrafish Embryos, Followed by Bacterial Dissemination and Establishment of Systemic Infection. INFECT IMMUN, 78(4), 1495-1508.
- Pivotal Advance: Pharmacological manipulation of inflammation resolution during spontaneously resolving tissue neutrophilia in the zebrafish.. J Leukoc Biol, 87(2), 203-212.
- Oxazolone-Induced Enterocolitis in Zebrafish Depends on the Composition of the Intestinal Microbiota. GASTROENTEROLOGY, 137(5), 1757-1767.
- Using in vivo zebrafish models to understand the biochemical basis of neutrophilic respiratory disease.. Biochem Soc Trans, 37(Pt 4), 830-837.
- Using Transgenic Zebrafish To Screen for Small Molecules That Promote Resolution of Neutrophilic Inflammation.. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 179.
- Pathways regulating lipopolysaccharide-induced neutrophil survival revealed by lentiviral transduction of primary human neutrophils.. Immunology, 127(2), 249-255.
- Evolution of the inflammatory response in vertebrates: Fish TNF-alpha is a powerful activator of endothelial cells but hardly activates phagocytes. J IMMUNOL, 181(7), 5071-5081.
- A novel vertebrate model of Staphylococcus aureus infection reveals phagocyte-dependent resistance of zebrafish to non-host specialized pathogens.. Cell Microbiol, 10(11), 2312-2325.
- Modeling inflammation in the zebrafish: how a fish can help us understand lung disease.. Exp Lung Res, 33(10), 549-554.
- Unraveling tissue regeneration pathways using chemical genetics.. J Biol Chem, 282(48), 35202-35210.
- Identifying and hurdling obstacles to translational research.. Nat Rev Immunol, 7(1), 77-82.
- A transgenic zebrafish model of neutrophilic inflammation.. Blood, 108(13), 3976-3978.
- Granulocyte apoptosis in the pathogenesis and resolution of lung disease.. Clin Sci (Lond), 110(3), 293-304.
- Expression of pro-apoptotic Bfk isoforms reduces during malignant transformation in the human gastrointestinal tract.. FEBS Lett, 579(17), 3646-3650.
- Three novel Bid proteins generated by alternative splicing of the human Bid gene.. J Biol Chem, 279(4), 2846-2855.
- Acceleration of Human Neutrophil Apoptosis by Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL). Clinical Science, 104(s49), 58P-58P.
- Acceleration of human neutrophil apoptosis by TRAIL.. J Immunol, 170(2), 1027-1033.
- Identification and Characterisation of 3 Novel Bid Isoforms. Clinical Science, 103(s47), 54P-54P.
- Expression and Function of Novel Isoforms of the Apoptosis Regulating Protein, Bid, in Human Neutrophils. Clinical Science, 101(s45), 2P-2P.
- Inflammatory neutrophils retain susceptibility to apoptosis mediated via the Fas death receptor.. J Leukoc Biol, 67(5), 662-668.
- Apoptosis and the regulation of neutrophil lifespan.. Biochem Soc Trans, 27(6), 802-807.
- Mechanisms of Neutrophil Apoptosis and the Regulation of Inflammation. Biochemical Society Transactions, 27(5), A134-A134.
- Correction: 15-keto-prostaglandin E2 activates host peroxisome proliferator-activated receptor gamma (PPAR-γ) to promote Cryptococcus neoformans growth during infection. PLOS Pathogens, 16(11), e1009058-e1009058.
Book chapters
- Measuring inflammatory cell migration in the zebrafish. (pp. 261-275).
Conference proceedings
- 6 Assessing the role of PET-CT in diagnosing cardiac sarcoid: a retrospective review of referral pathways and diagnostic outcomes in a newly established tertiary centre service. BSCI BSCCT Abstracts Conference 2–4 April (pp A2.3-A2)
- Hyperpolarised 129-xenon diffusion-weighted MRI in interstitial lung disease. Imaging (pp PA3157-PA3157)
- Longitudinal change in hyperpolarised 129-xenon MR spectroscopy in interstitial lung disease. Imaging (pp PA3158-PA3158)
- S125 Quantitative CT and hyperpolarised 129-xenon diffusion-weighted MRI in interstitial lung disease. Thorax, Vol. 74(Suppl 2) (pp a79). Westminster, London, United Kingdom, 4 December 2019 - 4 December 2019. View this article in WRRO
- Balancing the Immune Response in Cystic Fibrosis: Using Zebrafish Models of Inflammation to Uncover New Therapeutic Approaches. A62. MUCUS, MUCINS, MUCOCILIARY UNIT IN LUNG DISEASE (pp A7331-A7331)
- S2- O13: Neutrophils activated with Hif-1 alpha are protective in zebrafish tuberculosis in vivo models. European Journal of Clinical Investigation, Vol. 49(S1) (pp 67-68). Coimbra, Portugal, 22 May 2019 - 22 May 2019. View this article in WRRO
- The role of augmenting material in Staphylococcus aureus interaction with macrophages. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Vol. 49 (pp 205-205)
- Lc3-associated phagocytosis provides an intracellular niche for Staphylococcus aureus in neutrophils. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Vol. 49 (pp 203-204)
- Probing diffusion and perfusion in idiopathic pulmonary fibrosis with hyperpolarised xenon and dynamic contrast-enhanced magnetic resonance imaging. Thorax, Vol. 73(Suppl 4) (pp A44-A45). London, UK, 5 December 2018 - 5 December 2018. View this article in WRRO
- Estimation of hidden chemoattractant field from observed cell migration patterns. IFAC-PapersOnLine, Vol. 51(15) (pp 766-771). Stockholm, Sweden, 9 July 2018 - 9 July 2018. View this article in WRRO
- Balancing the immune response in cystic fibrosis: using zebrafish models of inflammation to uncover new therapeutic approaches. Journal of Cystic Fibrosis, Vol. 17(S3) (pp 58-59). Belgrade, Serbia, 6 June 2018 - 6 June 2018. View this article in WRRO
- Dynamic Contrast-Enhanced Magnetic Resonance Imaging for the Evaluation of Perfusion Heterogeneity in Idiopathic Pulmonary Fibrosis. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol. 197
- Late breaking abstract - Hyperpolarised gas MRI demonstrates sub-clinical progression in IPF over 6 months. European Respiratory Journal, Vol. 50(Suppl 61). Milan, Italy, 9 September 2017 - 9 September 2017. View this article in WRRO
- Polymersomes for targeting and eradicating intracellular parasites. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 253
- Synthesis of novel tanshinones for probing the inflammatory response in zebrafish. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 254
- Co-operation during Staphylococcus aureus pathogenesis. Journal of Infection, Vol. 71(6) (pp 684-684)
- SAT0477 Rituximab Treatment for Connective Tissue Disease Associated Interstitial Lung Disease: A Retrospective Case Series. Annals of the Rheumatic Diseases, Vol. 74 (pp 833-833)
- Hypoxia signaling modulates neutrophil nitric oxide in a zebrafish tuberculosis model. EUROPEAN RESPIRATORY JOURNAL, Vol. 44
- THE CREATION OF A SIMULATED PAN-DEANERY MEDICAL THORACOSCOPY AND INDWELLING PLEURAL CATHETER COURSE. THORAX, Vol. 68 (pp A108-A109)
- A Bayesian framework for identifying cell migration dynamics. 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) (pp 3455-3458), 3 July 2013 - 7 July 2013.
- A Bayesian framework for identifying cell migration dynamics. 2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) (pp 3459-3462)
- HYPOXIA-INDUCIBLE FACTOR 2α REGULATES NEUTROPHILIC INFLAMMATION IN HUMANS, MICE AND ZEBRAFISH. THORAX, Vol. 67 (pp A1-A1)
- STANDARDISATION OF BRONCHOSCOPY TRAINING ACROSS YORKSHIRE AND HUMBER DEANERY. THORAX, Vol. 67 (pp A171-A172)
- Macrophage-delivered signals drive inflammation resolution in an in vivo zebrafish model. IMMUNOLOGY, Vol. 137 (pp 255-255)
- Local affine texture tracking for serial registration of zebrafish images.. ISBI (pp 434-437)
- HIF-2± Regulates Neutrophilic Inflammation In Humans, Mice And Zebrafish. C31. REGULATION OF LUNG INFLAMMATION (pp A4173-A4173)
- Exploring neutrophil behaviour in a zebrafish model of inflammation through the generation of novel parameters using MatLab algorithms. IMMUNOLOGY, Vol. 137 (pp 254-254)
- Assessment of exercise capacity following continuous positive airway pressure therapy in patients with obstructive sleep apnoea/hypopnoea syndrome using the incremental shuttle walk test (ISWT). JOURNAL OF SLEEP RESEARCH, Vol. 21 (pp 347-347)
- INDIVIDUAL CELL TRACKING IN A TRANSGENIC ZEBRAFISH INFLAMMATION MODEL REVEALS THE FATES OF INFLAMMATORY NEUTROPHILS DURING INFLAMMATION RESOLUTION. THORAX, Vol. 65 (pp A56-A56)
- Wiskott Aldrich syndrome protein knockdown enhances collateral vessel formation in zebrafish. EUROPEAN HEART JOURNAL, Vol. 31 (pp 622-622)
- SCREENING FOR NOVEL ANTI-INFLAMMATORY THERAPIES IN A WHOLE ANIMAL IN VIVO MODEL. THORAX, Vol. 64 (pp A42-A43)
- Using transgenic zebrafish models to help understand inflammatory lung disease. THORAX, Vol. 62 (pp A116-A117)
- Lentivirus: A tool for the study of neutrophil biology. JOURNAL OF LEUKOCYTE BIOLOGY (pp 36-36)
- Regulation of granulocyte apoptosis by 3 novel isoforms of the BCL-2 protein, Bid. THORAX, Vol. 58 (pp 31-31)
Preprints
- Verapamil limits inflammation by restoring VGCC-driven epithelial Ca2+ in models of cystic fibrosis, Cold Spring Harbor Laboratory.
- Biallelic mutations in SUPV3L1 cause an inherited neurodevelopmental disorder with variable leukodystrophy due to aberrant mitochondrial double stranded RNA processing, Springer Science and Business Media LLC.
- Curcumin-mediated NRF2 induction limits inflammatory damage in preclinical models of cystic fibrosis, Cold Spring Harbor Laboratory.
- Pulses of Class I PI3kinase activity identify the release and recapture of prey from neutrophil phagosomes, Cold Spring Harbor Laboratory.
- Macrophage transplantation rescues RNASET2-deficient leukodystrophy by replacing deficient microglia in a zebrafish model, Cold Spring Harbor Laboratory.
- Determining the importance of the stringent response for methicillin-resistantStaphylococcus aureusvirulence using a zebrafish model of infection, Cold Spring Harbor Laboratory.
- WITHDRAWN: Biallelic mutation ofSUPV3L1causes an inherited leukodystrophy-associated neurodevelopmental disorder due to aberrant mitochondrial double stranded RNA processing, Cold Spring Harbor Laboratory.
- Analysis of the Distal Urinary Tract in Larval and Adult Zebrafish Reveals Unrecognized Homology to the Human System, Cold Spring Harbor Laboratory.
- A p21-GFP zebrafish model of senescence for rapid testing of senolyticsin vivo, Cold Spring Harbor Laboratory.
- The EGFR/ErbB inhibitor neratinib modifies the neutrophil phosphoproteome and promotes apoptosis and clearance by airway macrophages, Cold Spring Harbor Laboratory.
- A subset of gut leukocytes have telomerase-dependent “hyper-long” telomeres and require telomerase for function in zebrafish, Cold Spring Harbor Laboratory.
- Roscovitine exacerbates Mycobacterium abscessus infection by reducing NADPH oxidase-dependent neutrophil trafficking, Cold Spring Harbor Laboratory.
- A zebrafish reporter line reveals immune and neuronal expression of endogenous retrovirus, Cold Spring Harbor Laboratory.
- Blood vessel occlusion byCryptococcus neoformansis a mechanism for haemorrhagic dissemination of infection, Cold Spring Harbor Laboratory.
- Deletion ofcftrleads to an excessive neutrophilic response and defective tissue repair in a zebrafish model of sterile inflammation, Cold Spring Harbor Laboratory.
- Endogenous pioneer neutrophils release NETs during the swarming response in zebrafish, Cold Spring Harbor Laboratory.
- PGE2production at sites of tissue injury promotes an anti-inflammatory neutrophil phenotype and determines the outcome of inflammation resolutionin vivo, Cold Spring Harbor Laboratory.
- Mycophenolate mofetil increases susceptibility to opportunistic fungal infection independent of lymphocytes, Cold Spring Harbor Laboratory.
- Targeting mononuclear phagocytes for eradicating intracellular parasites, Cold Spring Harbor Laboratory.
- 15-keto-prostaglandin E2activates host peroxisome proliferator-activated receptor gamma (PPAR-γ) to promoteCryptococcus neoformansgrowth during infection, Cold Spring Harbor Laboratory.
- The CXCL12/CXCR4 signalling axis retains neutrophils at inflammatory sites in zebrafish.
- Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection. PLoS Pathogens, 18(4). View this article in WRRO
- Professional activities and memberships
-
- Director, The Bateson Centre.
- REMOVE Director, MRC Doctoral Training Partnership, Discovery Medicine North.
- Deputy Director and Sheffield Lead, MRC SHIELD Antimicrobial Research Consortium.
- REMOVE MRC Programme Grant Holder.
- REMOVE: Current sponsor of 3 Wellcome Trust/Royal Society Sir Henry Dale Fellows, 1 Wellcome Trust Clinical Training Fellow and 1 European Leukodystrophy Association Fellow.
- Remove: Board Member and co-chair of the Trainee Programme, Zebrafish Disease Models Society.
- Principal Organiser, Neutrophil in Immunity Meeting 2021 and Zebrafish Disease Models meeting 2022.
- Research Excellence Framework, subpanel 1.