Dr Anthony J Bullock
BSc, PhD
Department of Materials Science and Engineering
Research Associate
- Profile
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I joined the Tissue Engineering group in 2000 as a senior postdoctoral research associate, involved in interdisciplinary work in the development of tissue engineering of skin and wound healing.
I obtained my degree in Biomedical Technology at Sheffield City Polytechnic in 1992, including a year-long industrial placement at Porton Down, learning cell culture and rudimentary experiments on in vitro skin models. I then researched the role of protein kinase C in calcium signalling during agonist-induced platelet activation, and development of partial agonists to the IP3 receptor at the University of Bath.
My PhD in physiology, at the Physiological Laboratory in Liverpool, focussed on species and developmental changes in response to hypoxia in the smooth muscle of the ureter. I then became a research associate at Liverpool University studying the effects of bacterial infection on the human ureter.
- Research interests
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I’m currently working on an interdisciplinary project funded by Proctor and Gamble to investigate inflammatory cytokines and their effect on tissue-engineered skin models. Data on the effects on the morphology of the developing epidermis is being used to develop an agent-based computer model of human epidermis.
This interdisciplinary research incorporates the following areas:-
- Tissue engineering of skin
- Inflammation and its effects on epidermal morphology
- Agent-based computer modelling
- Publications
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Journal articles
- Spatiotemporal release of VEGF from biodegradable polylactic-co-glycolic acid microspheres induces angiogenesis in chick chorionic allantoic membrane assay. International Journal of Pharmaceutics, 561, 236-243.
- Bacteria induced pH changes in tissue-engineered human skin detected non-invasively using Raman confocal spectroscopy. Applied Spectroscopy Reviews, 1-14.
- Characterisation of structural changes in collagen with Raman spectroscopy. Applied Spectroscopy Reviews, 54(6), 509-542.
- Visualisation of the insertion of a membrane for the treatment of preterm rupture of fetal membranes using a synthetic model of a pregnant uterus. Journal of Biomaterials Applications, 33(2), 234-244.
- Stem Cell-Based Tissue-Engineered Laryngeal Replacement. Stem Cells Transl Med, 6(2), 677-687. View this article in WRRO
- Developing repair materials for stress urinary incontinence to withstand dynamic distension. PLoS One, 11(3). View this article in WRRO
- Development of an implantable synthetic membrane for the treatment of preterm premature rupture of fetal membranes. Journal of Biomaterials Applications, 30(7), 995-1003.
- Production of ascorbic acid releasing biomaterials for pelvic floor repair. Acta Biomaterialia, 29, 188-197. View this article in WRRO
- Application of Tissue Engineering to Pelvic Organ Prolapse and Stress Urinary Incontinence. LUTS: Lower Urinary Tract Symptoms, 7(2), 63-70. View this article in WRRO
- Tissue engineered buccal mucosa for urethroplasty: Progress and future directions. Advanced Drug Delivery Reviews, 82-83, 69-76. View this article in WRRO
- Biomaterials for Pelvic Floor Reconstructive Surgery: How Can We Do Better?. BioMed Research International, 2015, 1-20. View this article in WRRO
- The effect of ascorbic acid and fluid flow stimulation on the mechanical properties of a tissue engineered pelvic floor repair material. Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, 228(9), 867-875.
- Development of a Basement Membrane Substitute Incorporated Into an Electrospun Scaffold for 3D Skin Tissue Engineering. Journal of Biomaterials and Tissue Engineering, 4(9), 686-692.
- Acute In Vivo Response to an Alternative Implant for Urogynecology. BioMed Research International, 2014. View this article in WRRO
- Surface functionalization of electro-spun Poly(L) Lactic Acid scaffolds with heparin to induce angiogenesis. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 8, 169-169.
- Developing a tissue engineered repair material for treatment of stress urinary incontinence and pelvic organ prolapse-which cell source?. Neurourol Urodyn, 33(5), 531-537.
- Glucomannan-poly(N-vinyl pyrrolidinone) bicomponent hydrogels for wound healing. Journal of Materials Chemistry B.
- Development of a one-step approach for the reconstruction of full thickness skin defects using minced split thickness skin grafts and biodegradable synthetic scaffolds as a dermal substitute.. Burns.
- Tissue engineering airway mucosa: a systematic review.. Laryngoscope.
- Development of bilayer and trilayer nanofibrous/microfibrous scaffolds for regenerative medicine. Biomaterials Science, 1(9), 942-951.
- Comparison of candidate scaffolds for tissue engineering for stress urinary incontinence and pelvic organ prolapse repair.. BJU Int, 112(5), 674-685.
- Skin stem cell hypotheses and long term clone survival--explored using agent-based modelling.. Sci Rep, 3, 1904. View this article in WRRO
- High molecular weight plant heteropolysaccharides stimulate fibroblasts but inhibit keratinocytes.. Carbohydr Res, 375, 90-99.
- Tissue engineering as a potential alternative or adjunct to surgical reconstruction in treating pelvic organ prolapse: comment on Boennelycke et al.. Int Urogynecol J, 24(5), 881.
- Electrospun biaxially distensible bilayer membranes to treat cleft palate. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 6, 340-340.
- Mesh social networking: a patient-driven process.. BJU Int, 109(12), E45-E46.
- Examining the effect of subjecting a tissue engineered pelvic floor repair material to fixed and dynamic stress during its production to improve the biomechanical properties for future use in stress urinary incontinence and pelvic organ prolapse. EUROPEAN UROLOGY SUPPLEMENTS, 11(1), E1065-EU223.
- Postproduction processing of electrospun fibres for tissue engineering.. J Vis Exp(66).
- Are biomechanical properties predictive of the success of prostheses used in stress urinary incontinence and pelvic organ prolapse? A systematic review.. Neurourol Urodyn, 31(1), 13-21.
- Methods to reduce the contraction of tissue-engineered buccal mucosa for use in substitution urethroplasty.. Eur Urol, 60(4), 856-861.
- THE EFFECT OF SCAFFOLD RESTRAINT ON THE PROPERTIES OF TISSUE ENGINEERED PROSTHESES BEING DEVELOPED FOR USE IN STRESS URINARY INCONTINENCE (SUI) AND PELVIC ORGAN PROLAPSE (POP). EUR UROL SUPPL, 10(2), 291-291.
- Investigation of seven candidate scaffolds for the production of an autologous tissue engineered connective tissue for use in stress urinary incontinence and pelvic organ prolapse. European Cells and Materials, 22(SUPPL.3), 3.
- Creating a tissue engineered autologous prosthesis for use in stress urinary incontinence (SUI) and pelvic organ prolapse (POP) repair. European Cells and Materials, 22(SUPPL.2), 65.
- Developing biodegradable scaffolds for tissue engineering of the urethra.. BJU Int, 107(2), 296-302.
- DEVELOPING AN AUTOLOGOUS TISSUE ENGINEERED PROSTHESIS FOR USE IN STRESS URINARY INCONTINENCE AND PELVIC ORGAN PROLAPSE. INT UROGYNECOL J, 21, S325-S326.
- Development of a calcium-chelating hydrogel for treatment of superficial burns and scalds.. Regen Med, 5(1), 55-64.
- Co-culture of intestinal epithelial and stromal cells in 3D collagen-based environments. REGEN MED, 4(3), 397-406.
- METHODS TO REDUCE THE CONTRACTION OF TISSUE ENGINEERED BUCCAL MUCOSA FOR USE IN SUBSTITUTION URETHROPLASTY. EUR UROL SUPPL, 8(4), 186-186.
- EVALUATION OF STERILIZATION METHODS ON BIOLOGICAL AND MECHANICAL PROPERTIES OF A SYNTHETIC BIODEGRADABLE ELECTROSPUN SCAFFOLD FOR THE CREATION OF TISSUE ENGINEERED BUCCAL MUCOSA FOR CLINICAL USE. EUR UROL SUPPL, 8(4), 185-185.
- Inhibition of keratinocyte-driven contraction of tissue-engineered skin in vitro by calcium chelation and early restraint but not submerged culture.. J Burn Care Res, 29(2), 369-377.
- Tissue engineered, ex vivo, and in vivo models for the study of polymersomes diffusion across the skin. 8th World Biomaterials Congress 2008, 1, 414.
- Decellularization and sterilization of porcine urinary bladder matrix for tissue engineering in the lower urinary tract.. Regen Med, 3(2), 145-156.
- Randomized, controlled, single-blind study on use of autologous keratinocytes on a transfer dressing to treat nonhealing diabetic ulcers.. Regen Med, 2(6), 887-902.
- Transglutaminase inhibitors induce hyperproliferation and parakeratosis in tissue-engineered skin.. Br J Dermatol, 156(2), 247-257.
- The effect of induced biphasic pulsed currents on re-epithelialization of a novel wound healing model.. Bioelectromagnetics, 28(1), 31-41.
- Use of an in vitro model of tissue-engineered skin to investigate the mechanism of skin graft contraction.. Tissue Eng, 12(11), 3119-3133.
- Investigation of keratinocyte regulation of collagen I synthesis by dermal fibroblasts in a simple in vitro model.. Br J Dermatol, 154(3), 401-410.
- Use of human fibroblasts in the development of a xenobiotic-free culture and delivery system for human keratinocytes.. Tissue Eng, 12(2), 245-255.
- Tissue-engineered buccal mucosa for substitution urethroplasty.. BJU Int, 93(6), 807-811.
- Developmental and species differences in the response of the ureter to metabolic inhibition.. Pflugers Arch, 436(3), 443-448.
- Developmental changes in intracellular pH buffering power in smooth muscle. Pflugers Arch., 435(4), 575-577.
- The effects of metabolic inhibition on force, Ca2+ and pHi in guinea-pig ureteric smooth muscle. Pflugers Arch., 435(2), 240-246.
- The role of the sarcolemmal Ca(2+)-ATPase in the pH transients associated with contraction in rat smooth muscle. J.Physiol, 505 ( Pt 2), 329-336.
- Enantiomers of myo-inositol-1,3,4-trisphosphate and myo-inositol-1,4,6 -trisphosphate: stereospecific recognition by cerebellar and platelet myo-inositol-1,4,5-trisphosphate receptors. Mol.Pharmacol., 50(5), 1223-1230.
- A role for protein phosphorylation in modulating Ca2+ elevation in rabbit platelets treated with thapsigargin.. Biochem J, 313, 83-89.
- Myo-inositol 1,4,6-trisphosphorothioate and myo-inositol 1,3,4-trisphosphorothioate: New synthetic Ca2+-mobilising partial agonists at the inositol 1,4,5-trisphosphate receptor. Bioorganic and Medicinal Chemistry Letters, 5(3), 203-208.
- Developing improved tissue-engineered buccal mucosa grafts for urethral reconstruction. Canadian Urological Association Journal, 12(5), E234-42.
- Application of layer-by-layer coatings to tissue scaffolds – development of an angiogenic biomaterial. J. Mater. Chem. B, 2(34), 5558-5568.
Chapters
- pH and Smooth Muscle Function: Regulation and Functional Effects. In Taylor EW, Eggington, S & Raven J (Ed.), Regulation of Tissue pH in Plants and Animals: A Reappraisal of Current Techniques Cambridge, UK: Cambridge University Press.
Conference proceedings papers
- Estradiol Releasing Scaffolds for the Treatment of Pelvic Floor Disorders. TISSUE ENGINEERING PART A, Vol. 21 (pp S202-S202)
- Nine Year Follow Up on Tissue Engineered Buccal Mucosa used for Urethroplasty - What have we Learnt?. TISSUE ENGINEERING PART A, Vol. 21 (pp S34-S34)
- Physiological and Biological Effects of Konjac Glucomannan Hydrogels on Human Tissue Engineered Skin. TISSUE ENGINEERING PART A, Vol. 21 (pp S304-S304)
- Composite Materials for Pelvic Floor Repair Which Cope with Dynamic Distension and Support Cell Integration. TISSUE ENGINEERING PART A, Vol. 21 (pp S215-S215)
- RESPONSE OF SCAFFOLD MATERIALS DESIGNED FOR TREATMENT OF STRESS URINARY INCONTINENCE (SUI) AND PELVIC ORGAN PROLAPSE (POP) TO DISTENSION. NEUROUROLOGY AND URODYNAMICS, Vol. 34 (pp S291-S292)
- Improving elastogenesis in a tissue engineered repair material for pelvic floor surgery. BRITISH JOURNAL OF SURGERY, Vol. 101 (pp 60-60)
- ACUTE HOST RESPONSE AGAINST A NOVEL TISSUE ENGINEERED REPAIR MATERIAL FOR THE SURGICAL MANAGMENT OF STRESS URINARY INCONTINENCE AND PELVIC ORGAN PROLAPSE. NEUROUROLOGY AND URODYNAMICS, Vol. 32(6) (pp 555-556)
- Biomechanical optimisation of a tissue engineered prosthesis for pelvic floor repair. BRITISH JOURNAL OF SURGERY, Vol. 100 (pp 66-66)
- DEVELOPING AN AUTOLOGOUS ENGINEERED CONNECTIVE TISSUE USING A BIODEGRADABLE SCAFFOLD FOR THE TREATMENT OF STRESS URINARY INCONTINENCE AND PELVIC ORGAN PROLAPSE. NEUROUROLOGY AND URODYNAMICS, Vol. 31(6) (pp 917-919)
- Assessment of a variable stress rig to improve the properties of a tissue engineered prosthesis for use in stress urinary incontinence and pelvic organ prolapse. BRITISH JOURNAL OF SURGERY, Vol. 99 (pp 58-59)
- Engineering a novel tissue engineered autologus prosthesis for use in stress urinary incontinence (SUI) and pelvic organ prolapse (POP) repair. BRITISH JOURNAL OF SURGERY, Vol. 98(6) (pp E6-E6)
- Increasing collagen production and contraction with ascorbate in a tissue engineered autologus prosthesis for use in the treatment of stress urinary incontinence (SUI) and pelvic organ prolapse (POP). BRITISH JOURNAL OF SURGERY, Vol. 98(6) (pp E7-E7)
- Creating a tissue engineered prosthesis for stress urinary incontinence and pelvic organ prolapse, which scaffold?. BRITISH JOURNAL OF SURGERY, Vol. 98 (pp 4-5)
- Development of aseptically produced scaffolds for substitution urethroplasty. BRITISH JOURNAL OF SURGERY, Vol. 98 (pp 54-54)
- COMPARATIVE INVESTIGATION OF SEVEN CANDIDATE SCAFFOLDS FOR THE PRODUCTION OF AN AUTOLOGOUS TISSUE ENGINEERED CONNECTIVE TISSUE FOR USE IN STRESS URINARY INCONTINENCE AND PELVIC ORGAN PROLAPSE. NEUROUROLOGY AND URODYNAMICS, Vol. 30(6) (pp 868-870)
- A NOVEL TISSUE ENGINEERING APPROACH FOR CREATING PROSTHESES FOR THE TREATMENT OF STRESS URINARY INCONTINENCE (SUI) & PELVIC ORGAN PROLAPSE (POP). EUROPEAN UROLOGY SUPPLEMENTS, Vol. 9(2) (pp 328-328)
- DEVELOPING AN AUTOLOGOUS TISSUE ENGINEERED PROSTHESIS FOR USE IN STRESS URINARY INCONTINENCE AND PELVIC ORGAN PROLAPSE. NEUROUROLOGY AND URODYNAMICS, Vol. 29(6) (pp 1129-1130)
- Investigation of the mechanism of contraction of tissue-engineered skin. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, Vol. 86(3) (pp A27-A28)
- Regulation of the MYC network in the progression to oesophageal adenocarcinoma. GUT, Vol. 54 (pp A87-A87)
- Reducing the risks in the production of tissue engineered buccal mucosa for urethral reconstruction.. European Association of Urologists. Milan, Italy, 15 March 2013 - 19 March 2013.
- Metabolic inhibition in neonatal and adult guinea pig ureter.. Fetal and Neonatal Physiology Society. Arica, Chile, 14 August 1996 - 18 August 1996.
- pH regulation in neonate and adult guinea pig ureteric smooth muscle. Fetal and Neonatal Physiology Society. St. Margherita Ligure, Italy, 8 June 1997 - 11 June 1997.
- The effects of metabolic inhibition on force and intracellular calcium in guinea pig ureter.. The Physiology Society. Edinburgh, 2 July 1996 - 6 July 1996.
- Spatiotemporal release of VEGF from biodegradable polylactic-co-glycolic acid microspheres induces angiogenesis in chick chorionic allantoic membrane assay. International Journal of Pharmaceutics, 561, 236-243.