Dr Helen Marriott

PhD

Department of Infection, Immunity and Cardiovascular Disease

Lecturer in Respiratory Infection

h.m.marriott@sheffield.ac.uk
+44 114 215 9537

Full contact details

Dr Helen Marriott
Department of Infection, Immunity and Cardiovascular Disease
The Medical School
Beech Hill Road
Sheffield
S10 2RX
Profile

I joined the University of Sheffield in 1995 from the Department of Respiratory Physiology, Papworth Hospital, Cambridge.  From 1995 to 2001 my research was on the pulmonary circulation, focusing on animal models of pulmonary hypertension.  This formed the basis of my PhD, "Pulmonary hypertension: susceptibility and treatment in rat models".

In 2001 I joined Professor David Dockrell´s group as a post-doctoral research associate investigating the role of macrophage apoptosis in pneumococcal infection.

In 2005 I was awarded an independent fellowship from the British Lung Foundation to investigate the effects of influenza A virus on macrophage innate immune function.

In 2015 I was appointed Lecturer in Respiratory Infection.

Since 2016 I have been the academic lead of the Medical School Flow Cytometry Core Facility.

Research interests

My main research interest is in the role of macrophages in host defense against respiratory pathogens, in particular Streptococcus pneumoniae. I am also interested in the effect of influenza A virus on macrophage function, its effect on the regulation of macrophage apoptosis and how this may lead to increased susceptibility to bacterial superinfections. I have been using a variety of in vitro, in vivo models and computational models to support this research.


Current projects

Re-engaging antimicrobial killing by macrophages to combat antimicrobial resistance.

I was awarded an Innovation grant from the MRC as part of the cross research council initiative Tackling Antimicrobial Resistance to identify new targets for novel antibacterials. We have described macrophage apoptosis associated killing as an alternative means by which macrophages kill bacteria, however we have found that this is subverted in some patients at increased risk of bacterial infections, such as Chronic Obstructive Lung Disease. In addition some bacteria, notably S. aureus, can prevent macrophage apoptosis associated killing to survive within macrophages. In this grant we will screen and develop compounds to engage macrophage apoptosis as a means to improve bacterial killing and modify inflammation. By targeting the host, rather than the pathogen, we anticipate this will avoid antimicrobial resistance. This concept is a focus of the MRC Collaborative Grant, Optimising Innate Host Defence to Combat Antimicrobial Resistance of which I am a co-investigator.

Molecular analysis of the effects of influenza A virus on macrophage innate immune function (Wellcome Trust VIP award and Personal fellowship from the British Lung Foundation).

The aim of my fellowship was to characterise the effect of influenza A virus on macrophage function, with particular emphasis on its effect on the regulation of macrophage apoptosis. I have established in vitro macrophage and in vivo murine models of IAV infection and shown that both human monocyte derived macrophages and murine macrophages undergo apoptosis after influenza A virus infection. I am investigating how this relates to cytokine production, generation of antimicrobial molecules and killing of bacteria.

Role of ubiquitination in regulation of macrophage viability after bacterial infection.

Macrophages do not undergo apoptosis after infection with S. aureus or N. meningitidis, and I have shown this is associated with an increase in the anti-apoptotic protein Mcl-1. I am currently investigating the mechanism behind this up-regulation focusing on the role of post-translational modifications, in particular ubiquitination, and identifying the bacterial factors responsible.

Publications

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Journal articles

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Journal articles

Chapters

Conference proceedings papers

  • Marsh EK, Prestwich EC, Marriott HM, Williams L, Hart AR, Muir CF, Parker LC, Jonker MR, Heijink IH, Timens W , Fife M et al (2018) S98 Pellino-1 regulates the responses of the airway to viral infection. The influence of influenza: from cells to humans RIS download Bibtex download
  • Braithwaite AT, Pickworth JA, Arnold ND, West LE, Iremonger JE, Thompson AA, Marriott HM & Lawrie A (2018) T6 Vascular smooth muscle derived TRAIL underlies pulmonary vascular remodelling in sugen 5416/hypoxia mice. BTS/BALR/BLF Early Career Investigators awards symposium RIS download Bibtex download
  • Dickinson RS, Thompson AAR, Thomson JP, Murphy F, Marriott HM, Tavares A, Willson J, Williams L, Lewis A, Forbes S , Stimson RH et al (2016) S104 Hypoxia preconditions the innate immune response to acute bacterial pulmonary infections. Thorax, Vol. 71(Suppl 3) (pp A61.2-A62) RIS download Bibtex download
  • Marsh EK, Prestwich EC, Marriott HM, Paiva C, Bennett JA, Jonker MR, Timens W, Heijink IH, Parker LC, Dockrell DH & Sabroe I (2016) The Role Of Pellino-1 In Modulating Signalling Pathways Controlling The Inflammatory Response To Viral Stimuli In Airway Epithelial Cells. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol. 193 RIS download Bibtex download
  • Gill SK, Marriott HM, Peachell PT & Immunity DI (2014) Prostaglandin E2 Activates Ep4 Receptors To Inhibit Pro-Inflammatory Cytokine Release From Human Lung Macrophages. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol. 189 RIS download Bibtex download
  • Bingle CD, Araujo B, Wallace WA, Marriott HM, Hirani N & Bingle L (2013) BPIFB1/LPLUNC1 IS A NOVEL MARKER FOR THE BRONCHIOLISED EPITHELIUM IN IPF. THORAX, Vol. 68 (pp A139-A139) RIS download Bibtex download
  • Thompson AAR, Marriott HM, Williams L, Shaw G, Hameed A, Parmar S, Preston JA, Simon MC, Johnson RS, Foster SJ , Dockrell DH et al (2013) HYPOXIA INDUCES HYPOTHERMIA AND SICKNESS BEHAVIOUR IN MICE FOLLOWING SUBCUTANEOUS INJECTION OF LIVE STAPHYLOCOCCUS AUREUS. THORAX, Vol. 68 (pp A140-A140) RIS download Bibtex download
  • Thompson AAR, Elks PM, Marriott HM, Higgins KR, Parmar S, Shaw G, Eamsamarng S, McGrath EE, Formenti F, Van Eeden FJ , Kinnula VL et al (2012) HYPOXIA-INDUCIBLE FACTOR 2 alpha REGULATES NEUTROPHILIC INFLAMMATION IN HUMANS, MICE AND ZEBRAFISH. THORAX, Vol. 67 (pp A1-A1) RIS download Bibtex download
  • Araujo B, Bingle L, Marriott H, Howie S, Wallace W, Hirani N & Bingle CD (2012) LPLUNC1 (BPIFB1) As A Novel Marker For The Abnormal Epithelium In Pulmonary Fibrosis. B28. EMERGING BIOMARKERS OF COMPLEX LUNG DISEASES RIS download Bibtex download
  • Thompson AAR, Elks PM, Marriott HM, Higgins KR, Shaw G, Parmar S, Formenti F, Van Eden FJ, Pugh CW, Sabroe I , Dockrell DH et al (2012) HIF-2± Regulates Neutrophilic Inflammation In Humans, Mice And Zebrafish. C31. REGULATION OF LUNG INFLAMMATION RIS download Bibtex download
  • McGrath EE, Lawrie A, Marriott H, Mercer PF, Cross SS, Chambers RC, Dockrell DH & Whyte MKB (2011) S111 The role of TNF-related apoptosis inducing ligand (TRAIL) in pulmonary fibrosis. Thorax, Vol. 66(Suppl 4) (pp A51-A52) RIS download Bibtex download
  • McGrath EE, Marriott H, Lawrie A, Cross S, Mercer P, Hellewell P, Chambers RC, Dockrell D & Whyte MK (2011) The Role Of TNF-Related Apoptosis Inducing Ligand (TRAIL) In Idiopathic Pulmonary Fibrosis (IPF). D14. INSIGHTS INTO PATHOGENESIS OF LUNG FIBROSIS AND GRANULOMAS RIS download Bibtex download
  • Marriott HM, Whyte M & Dockrell D (2011) Fas Ligand Contributes To The Pulmonary Response To Streptococcus Pneumoniae. A35. LESSONS IN LUNG INFECTIONS RIS download Bibtex download
  • Webster SJ, Daigneault M, Marriott HM, Bewley MA, Read RC & Dockrell DH (2010) MONOCYTE DEATH PROCESSES ARE SUBVERTED BY MENINGOCOCCI. J INFECTION, Vol. 61(6) (pp 517-518) RIS download Bibtex download
  • Daigneault M, Collini P, Marriott HM, Preston J, Mitchell T & Dockrell D (2010) Monocytes promote T-lymphocyte apoptosis following Streptococcus pneumoniae challenge. IMMUNOLOGY, Vol. 131 (pp 179-179) RIS download Bibtex download
  • Marriott HM & Dockrell DH (2010) Regulation Of Apoptosis In A549 Cells After Influenza A Virus Infection. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol. 181 RIS download Bibtex download
  • Marriott HM, Whyte MK & Dockrell DH (2009) Macrophage Apoptosis after Influenza A Virus Infection.. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol. 179 RIS download Bibtex download
  • Mcgrath EE, Marriott HM, Hellewell PG, Dockrell DH & Whyte MK (2007) The role of TNF-related apoptosis inducing ligand in acute lung injury. THORAX, Vol. 62 (pp A62-A62) RIS download Bibtex download
  • Marriott HM, Read RC, Whyte MKB & Dockrell DH (2007) Maintenance of macrophage viability after meningococcal infection. IMMUNOLOGY, Vol. 120 (pp 82-82) RIS download Bibtex download
  • Marriott HM, Hellewell PG, Whyte MK & Dockrell DH (2005) Apoptotic macrophages limit inflammation in pneumococcal pneumonia. JOURNAL OF LEUKOCYTE BIOLOGY (pp 77-77) RIS download Bibtex download
  • Eneser M, Barer GR, Marriott HM & Emery CJ (2002) Quantification of pulmonary haemodynamic and vascular changes in chronic hypoxia and recovery in rats. JOURNAL OF PHYSIOLOGY-LONDON, Vol. 544 (pp 116P-116P) RIS download Bibtex download
  • Eneser M, Marriott HM, Emery CJ & Higenbottam TW (2001) Reversal of pulmonary hypertension and vascular remodelling by an endothelin receptor antagonist in the chronic hypoxic rat model. THORAX, Vol. 56 (pp 75-76) RIS download Bibtex download
  • Marriott HM, Emery CJ & Barer GR (2001) Fawn-hooded rats and pulmonary hypertension: histological and haemodynamic comparison with Wistar rats. J PHYSIOL-LONDON, Vol. 533 (pp 80P-81P) RIS download Bibtex download
  • Emery CJ, Lal H, Marriot HM, Whittle BJR, Shorr R, Bentley M, Zhao X & Higenbottam TW (2000) Pulmonary vasodilation by airway delivered prostacyclin analogues, UT-15 & pegylated UT-15. THORAX, Vol. 55 (pp A35-A35) RIS download Bibtex download
  • Marriott HM, Smith APL, Gonzalez AM, Emery CJ & Higenbottam TW (1999) Chronic dexfenfluramine treatment reduces the levels of serotonin transporter mRNA in normoxic and chronically hypoxic Wistar and Fawn hooded rats.. THORAX, Vol. 54 (pp A40-A40) RIS download Bibtex download
  • Emery CJ, Marriott HM, Laude EA, Prevost L & Higenbottam TW (1999) Pulmonary vasoactivity of fenfluramines and selective serotonin reuptake inhibitors, fluoxetine and amitriptyline, in the rat. THORAX, Vol. 54 (pp A40-A40) RIS download Bibtex download
  • Marriott HM, Emery CJ & Higenbottam TW (1999) Inhalation of endothelin(A/B) receptor antagonist partially reverses chronic hypoxic pulmonary hypertension in the rat. THORAX, Vol. 54 (pp A40-A40) RIS download Bibtex download
  • Patel R, Marriott HM, Emery CJ, McCormack K, Smith APL & Higenbottam TW (1999) Anti-ET-1 oligonucleotides as a potential treatment for pulmonary hypertension.. THORAX, Vol. 54 (pp A39-A39) RIS download Bibtex download
  • Patel R, Marriott HM, Smith APL, Emery CJ & Higenbottam TW (1999) Transfer of phosphorothioate oligodeoxynucleotides from the airway to the perfusate in isolated perfused rat lungs. AM J RESP CRIT CARE, Vol. 159(3) (pp A168-A168) RIS download Bibtex download
  • Marriott HM, Smith APL, Gonzalez AM, Emery CJ & Higenbottam TW (1999) Chronic dexfenfluramine treatment reduces the levels of serotonin transporter mRNA in normoxic and chronically hypoxic wistar and fawn hooded rats.. AM J RESP CRIT CARE, Vol. 159(3) (pp A168-A168) RIS download Bibtex download
  • Emery CJ, Marriott HM, Laude EA, Bee D & Higenbottam TW (1999) Attenuated pulmonary and systemic vasodilator response to serotonin in the Fawn Hooded rat. AM J RESP CRIT CARE, Vol. 159(3) (pp A165-A165) RIS download Bibtex download
  • Emery CJ, Phillips HM, Marriott HM, Laude EA & Bee D (1998) Pulmonary and systemic action of 5-HT in the Fawn Hooded rat: a model of inherited pulmonary and systemic hypertension. J PHYSIOL-LONDON, Vol. 511P (pp 108P-109P) RIS download Bibtex download
  • Marriott HM, Smith APL, Emery CJ & Higenbottam TW (1998) Rapid increases in the levels of preproendothelin-1 mRNA in the isolated perfused rat lung exposed to acute hypoxia. Thorax, Vol. 53(SUPPL. 4) RIS download Bibtex download
  • Laude EA, Emery CJ, Marriott HM & Higenbottam TW (1998) Altered vascular reactivity to serotonin in the fawn hooded rat; a model of spontaneous pulmonary and systemic hypertension. Thorax, Vol. 53(SUPPL. 4) RIS download Bibtex download
  • Marriott H, Akamine S & Higenbottam TW (1997) The effect of dexfenfluramine on 5-hydroxytryptamine clearance in the porcine lung. Thorax, Vol. 52(SUPPL. 6) RIS download Bibtex download
  • Marriott HM, Laude EA, Emery CJ & Higenbottam TW (1997) Enhanced pulmonary vasoreactivity to serotonin in the Fawn hooded rat. Thorax, Vol. 52(SUPPL. 6) RIS download Bibtex download
Teaching activities

I contribute to several modules in the MSc in Molecular Medicine, I am module leader for the modules ‘Modulating Immunity’, ‘Molecular and Cellular Basis of Disease' and 'Pathogenicity of Viruses and Fungi' and am the lead for the Experimental Medicine pathway.

I contribute to teaching/supervision of students on the Medical School MBChB and Molecular Biology and Biotechnology BSc/ MBiolSci courses.

I also teach on the Postgraduate Induction course of the Faculty of Medicine, Dentistry and Health and am the unfair means officer for the Medical School (PGR).

Professional activities
  • Member of University of Sheffield Animal Welfare and Ethical Review Board / 3Rs Committee