Theory & Practise

IHC advancedAdvanced Immunohistochemistry

22-23 May 2018 – book now

This course is accredited by the Institute of Biomedical Science.

This course is the third module in the Theory & Practise series and will provide advanced training in immunohistochemistry (IHC) to those working in the NHS, Higher Education or within industry. The course will teach you the knowledge and confidence to manipulate complex multiple IHC staining techniques to fit applications within your workplace.

On completion of the course, delegates will understand:

  • How IHC can be linked to other techniques to enhance their research or diagnostic potential.
  • When to use different techniques to best demonstrate the signal of interest.
  • How IHC can be used as a powerful diagnostic tool.

On completion of the course, delegates will be able to:

  • Carry out a triple/multiple IHC staining technique.
  • Explain why the different reagents used in the technique were chosen.
  • Discuss the use of Image Analysis software.
  • Develop their own techniques/methods for a multiple IHC staining technique or use IHC combined with another technique.

Vector labsThe topics covered in this 2 day practical module include:

  • How multiple IHC techniques are used in research.
  • How IHC can combined with in situ hybridization
  • How IHC can be used with gene sequencing/Affymetrix
  • How multiple IHC is used as a diagnostic tool
  • How to use Image analysis software.

Is the course right for me?

The advanced IHC course is suitable for anyone who has a basic science background and wishes to further their understanding of theory and practical experience in current and popular immunohistochemical techniques. It will consist of a series of lectures and lab based practical sessions and is taught over two days. Refreshment breaks and lunches will be provided.
The course will provide you with an in depth knowledge of the more advanced uses of IHC and you will leave having gained the skills to perform and manipulate IHC on tissue to identify complex/three different antigens/cellular components in a single tissue section

22-23 May 2018 – book now

SITraN, Department of Neuroscience, The University of Sheffield, Sheffied, S10 2HQ, United Kingdom

The cost of this two day course is £525. For more information, contact:

Natalie Kennerley -, 0114 222 6600
Lynne Baxter –, 0114 2222272/51