Brain slice electrophysiology

We use brain slice electrophysiology to investigate the physiological properties of distinct brainstem cell types based on their intrinsic biophysical characteristics. This technique allows for the detailed investigation of neurons involved in processing and modulating sound frequency, including those specialised for high- or low-pitch signals.

This approach is useful in our research because it allows us to directly measure how individual brainstem neurons involved in auditory processing respond to stimuli and adapt under different conditions. By understanding the functional properties of these cells, we would be able to identify how their activity contributes to pitch perception and how this may be altered by factors such as aging, noise exposure, or genetic changes—ultimately helping to pinpoint mechanisms underlying hearing loss. 

Shown here are example images of a brainstem slice with a patch electrode targeting a neuron, alongside a current-clamp recording illustrating its electrical response.