RNAscope

RNAscope is an advanced in situ hybridisation technique that enables the precise detection of RNA molecules within individual cells in intact tissue. Unlike traditional methods, RNAscope offers high spatial resolution and sensitivity, allowing us to visualise gene expression patterns at the single-cell level.

In our research, we use RNAscope to map the expression of specific genes within the auditory system and to examine how these patterns change in response to factors such as noise exposure, aging, or genetic manipulation. This is particularly useful because it allows us to localise transcriptional changes to defined cell types and regions within the cochlea and brainstem, providing critical insight into the molecular mechanisms underlying auditory function and dysfunction.