Two-photon imaging
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Two-photon microscopy is a powerful imaging technique that enables high-resolution visualisation of fluorescent signals deep within tissue, making it ideal for both ex vivo and in vivo applications.
In our research, we use two-photon microscopy to monitor cells within the auditory system, tracking changes in fluorescence intensity that reflect spontaneous electrical activity during development and normal functioning following maturation. This approach is especially useful because it allows us to observe how neuronal activity patterns emerge and evolve in real time, providing crucial insights into the maturation of auditory circuits and the factors that influence normal and abnormal auditory development.
