Dr Helen Marriott PhD
Lecturer in Respiratory Infection
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield
Beech Hill Road
Tel: +44 (0)114 215 9537
Fax: +44 (0)114 271 1863
I joined the University of Sheffield in 1995 from the Department of Respiratory Physiology, Papworth Hospital, Cambridge. From 1995 to 2001 my research was on the pulmonary circulation, focusing on animal models of pulmonary hypertension. This formed the basis of my PhD, "Pulmonary hypertension: susceptibility and treatment in rat models".
In 2001 I joined Professor David Dockrell´s group as a post-doctoral research associate investigating the role of macrophage apoptosis in pneumococcal infection. In 2005 I was awarded an independent fellowship from the British Lung Foundation to investigate the effects of influenza A virus on macrophage innate immune function.
In 2015 I was appointed Lecturer in Respiratory Infection.
My main research interest is in the role of macrophages in host defense against respiratory pathogens, in particular Streptococcus pneumoniae. Additionally, I am interested in the effect of influenza A virus on macrophage function, its effect on the regulation of macrophage apoptosis and how this may lead to increased susceptibility to bacterial superinfections. I have been using a variety of in vitro and in vivo models and am currently developing computational models to support this research.
My main collaboration is with Professor David Dockrell. I also collaborate on murine in vivo models with Professor Moira Whyte, Dr Sarah Walmsley and Dr Colin Bingle. My work on the development of computational models is in collaboration with Dr Alex Best (Mathematics and Statistics), Professor Rod Smallwood (Computer Science) and Professor Mike Boots (University of Exeter).
My main teaching interest is in supporting the development of strong fundamental research skills in students. I contribute to several modules in the MSc in Molecular Medicine, I am module leader for the Experimental Medicine pathway modules 'Molecular and Cellular Basis of Disease' and 'Model Systems in Medical Research', and the Microbes and Infection pathway module 'Pathogenicity of Viruses and Fungi'. I also teach on the Postgraduate Induction course of the Faculty of Medicine, Dentistry and Health.
- Committee member of the British Association for Lung Research.
- Member of University of Sheffield ethical review panel/3Rs Committee.
- Computational models of macrophage innate immune responses to Staphylococcus aureus.
- Role of ubiquitination in regulation of macrophage viability after bacterial infection.
- The role of apoptosis in murine models of pneumococcal pneumonia.
For key publications see below. For a full list of publications click here.
- Alveolar Macrophage Apoptosis-Associated Bacterial Killing Helps Prevent Murine Pneumonia.. American Journal of Respiratory and Critical Care Medicine. View this article in WRRO
- Inability to sustain intraphagolysosomal killing of Staphylococcus aureus predisposes to bacterial persistence in macrophages. Cellular Microbiology, 18(1), 80-96. View this article in WRRO
- Pneumolysin Activates Macrophage Lysosomal Membrane Permeabilization and Executes Apoptosis by Distinct Mechanisms without Membrane Pore Formation. mBio, 5(5). View this article in WRRO
- Hypoxia-inducible factor 2α regulates key neutrophil functions in humans, mice and zebrafish. Blood, 123(3), 366-376.
- A decoy receptor 3 analogue reduces localised defects in phagocyte function in pneumococcal pneumonia.. Thorax, 67(11), 985-992. View this article in WRRO
- Monocytes regulate the mechanism of T-cell death by inducing Fas-mediated apoptosis during bacterial infection. PLoS Pathogens, 8(7), 30. View this article in WRRO
- Deficiency of tumour necrosis factor-related apoptosis-inducing ligand exacerbates lung injury and fibrosis.. Thorax, 67(9), 796-803. View this article in WRRO
- Interleukin-1β regulates CXCL8 release and influences disease outcome in response to Streptococcus pneumoniae, defining intercellular cooperation between pulmonary epithelial cells and macrophages.. Infect Immun, 80(3), 1140-1149. View this article in WRRO
- TNF-related apoptosis-inducing ligand (TRAIL) regulates inflammatory neutrophil apoptosis and enhances resolution of inflammation.. J Leukoc Biol, 90(5), 855-865. View this article in WRRO
- Proteomic evaluation and validation of cathepsin D regulated proteins in macrophages exposed to Streptococcus pneumoniae.. Mol Cell Proteomics, 10(6), M111.008193. View this article in WRRO
- Prolyl hydroxylase 3 (PHD3) is essential for hypoxic regulation of neutrophilic inflammation in humans and mice.. J Clin Invest, 121(3), 1053-1063. View this article in WRRO
- A cardinal role for cathepsin d in co-ordinating the host-mediated apoptosis of macrophages and killing of pneumococci.. PLoS Pathog, 7(1), e1001262. View this article in WRRO
- Distinct cell death programs in monocytes regulate innate responses following challenge with common causes of invasive bacterial disease.. J Immunol, 185(5), 2968-2979.
- The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages.. PLoS One, 5(1), e8668. View this article in WRRO
- Reactive oxygen species regulate neutrophil recruitment and survival in pneumococcal pneumonia.. Am J Respir Crit Care Med, 177(8), 887-895. View this article in WRRO
- Decreased alveolar macrophage apoptosis is associated with increased pulmonary inflammation in a murine model of pneumococcal pneumonia.. J Immunol, 177(9), 6480-6488. View this article in WRRO
- Inhibition of macrophage apoptosis by Neisseria meningitidis requires nitric oxide detoxification mechanisms.. Infect Immun, 74(1), 729-733.
- Dynamic changes in Mcl-1 expression regulate macrophage viability or commitment to apoptosis during bacterial clearance. J CLIN INVEST, 115(2), 359-368. View this article in WRRO
- Nitric oxide levels regulate macrophage commitment to apoptosis or necrosis during pneumococcal infection.. FASEB J, 18(10), 1126-1128. View this article in WRRO
- Alveolar macrophage apoptosis contributes to pneumococcal clearance in a resolving model of pulmonary infection.. J Immunol, 171(10), 5380-5388. View this article in WRRO