Dr Lynne Prince
Department of Infection, Immunity and Cardiovascular Disease
Lecturer
l.r.prince@sheffield.ac.uk
+44 114 215 9546
+44 114 215 9546
+44 114 271 1863
(Fax)
The Medical School
Full contact details
Dr Lynne Prince
Department of Infection, Immunity and Cardiovascular Disease
The Medical School
Beech Hill Road
Sheffield
S10 2RX
Department of Infection, Immunity and Cardiovascular Disease
The Medical School
Beech Hill Road
Sheffield
S10 2RX
- Profile
-
For enquiries, please contact – iicd-operational@sheffield.ac.uk
I graduated from Newcastle University in 1997 with a degree in Medical Microbiology and relocated to Sheffield to study for an MSc in pathological sciences (University of Sheffield, 1999). This was followed by my PhD entitled `The Regulation of Neutrophil Apoptosis by Bacterial Products´ (University of Sheffield, 2000-04) under the supervision of Professor Moira Whyte.
In 2004 I was awarded a University of Sheffield Russell Research Fellowship to study the molecular mechanisms of neutrophil activation and apoptosis.
- Research interests
-
Inflammation and inflammatory diseases such as chronic obstructive pulmonary disease, or rheumatoid arthritis are extremely difficult to treat effectively. During inflammation, inflammatory cells, in particular neutrophils and monocytes, are thought to be key mediators of both tissue damage and perpetuation of further inflammatory signalling.
My research focuses on the role of neutrophils during infection and inflammation, and how their inflammatory potential and function is regulated by apoptosis. I also study how the immune response is subverted by pathogens such as Staphylococcus aureus and Haemophilus influenzae, via the Florey Institute for host-pathogen interactions.
Current projects:
- 2018-2021 “Do ErbB inhibitors have therapeutic potential in the resolution of inflammatory disease?” Faculty of MDH Studentship (Kimberley Herman) co-supervisor: Prof Steve Renshaw.
- 2018-2021 “Investigating phagocyte defects in diabetes” IICD/SHIELD studentship (Rebecca Dowey) lead supervisor Prof Ian Sabroe.
- 2017-2021 “Using super-resolution microscopy to understand the intracellular fate of Staphylococcus aureus” Florey/MRC SHIELD PhD Studentship (Yin Xin Ho) co-supervisor: Dr Ashley Cadby.
- 2017-2021 “Find-me and eat me: understanding how signals from dying cells control and subvert macrophage behavior” MRC DiMeN PhD studentship (Olivier Tardy) lead supervisor: Dr Iwan Evans.
- 2014-ongoing “Effect of Hypoglycemia on Inflammatory Responses and the Response to Low Dose Endotoxemia in Humans. MRC Clinical Training Fellowship (Ahmed Iqbal) co-supervisor with: Prof Ian Sabroe, Prof Sheila Francis & Prof Simon Heller.
- 2014-ongoing: The role of Pellino-1 in host responses to Haemophilus influenzae (in collaboration with Prof Ian Sabroe and Prof Alison Condliffe).
I am grateful to the Medical Research Council and British Lung Foundation for supporting recent research.
- Publications
-
Show: Featured publications All publications
Featured publications
Journal articles
- Effect of Hypoglycemia on Inflammatory Responses and the Response to Low Dose Endotoxemia in Humans.. Journal of Clinical Endocrinology and Metabolism, 104(4), 1187-1199. View this article in WRRO
- Identification of Staphylococcus aureus Factors Required for Pathogenicity and Growth in Human Blood. Infection and immunity, 85(11). View this article in WRRO
- NR4A orphan nuclear receptor family members, NR4A2 and NR4A3, regulate neutrophil number and survival. Blood, 130, 1014-1025. View this article in WRRO
- A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism.. Sci Transl Med, 6(225), 225ra29. View this article in WRRO
- Roles of neutrophils in the regulation of the extent of human inflammation through delivery of IL-1 and clearance of chemokines.. J Leukoc Biol, 93(1), 7-19. View this article in WRRO
- Pellino-1 selectively regulates epithelial cell responses to rhinovirus.. J Virol, 86(12), 6595-6604.
- Staphylococcus aureus induces eosinophil cell death mediated by α-hemolysin.. PLoS One, 7(2), e31506. View this article in WRRO
- Pathways regulating lipopolysaccharide-induced neutrophil survival revealed by lentiviral transduction of primary human neutrophils.. Immunology, 127(2), 249-255.
- Ex vivo-expanded bone marrow CD34+ derived neutrophils have limited bactericidal ability.. Stem Cells, 26(10), 2552-2563.
- Subversion of a lysosomal pathway regulating neutrophil apoptosis by a major bacterial toxin, pyocyanin.. J Immunol, 180(5), 3502-3511. View this article in WRRO
- Impairment of apoptotic cell engulfment by pyocyanin, a toxic metabolite of Pseudomonas aeruginosa.. Am J Respir Crit Care Med, 177(1), 35-43. View this article in WRRO
- The role of interleukin-1beta in direct and toll-like receptor 4-mediated neutrophil activation and survival.. Am J Pathol, 165(5), 1819-1826.
- The role of neutrophil apoptosis in the resolution of acute lung injury in newborn infants. THORAX, 58(11), 961-967.
- Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span.. J Immunol, 170(10), 5268-5275.
- Induction of Neutrophil Apoptosis by the Pseudomonas aeruginosa Exotoxin Pyocyanin: A Potential Mechanism of Persistent Infection. Journal of Immunology, 168(4), 1861-1868. View this article in WRRO
- Macrophage Phenotype Is Associated with Disease Severity in Preterm Infants with Chronic Lung Disease. PLoS ONE, 9(8), e103059-e103059. View this article in WRRO
All publications
Journal articles
- The Epidermal Growth Factor Ligand Spitz Modulates Macrophage Efferocytosis, Wound Responses and Migration Dynamics During Drosophila Embryogenesis. Frontiers in Cell and Developmental Biology, 9, 636024.
- Isolation and high throughput flow cytometric apoptosis assay of human neutrophils to enable compound library screening. Bio-protocol, 10(11).
- Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation. eLife, 8. View this article in WRRO
- The CXCL12/CXCR4 signaling axis retains neutrophils at inflammatory sites in zebrafish. Frontiers in Immunology, 10. View this article in WRRO
- Pellino-1 regulates immune responses to Haemophilus influenzae in models of inflammatory lung disease. Frontiers in Immunology, 10. View this article in WRRO
- Effect of Hypoglycemia on Inflammatory Responses and the Response to Low Dose Endotoxemia in Humans.. Journal of Clinical Endocrinology and Metabolism, 104(4), 1187-1199. View this article in WRRO
- A genome-wide screen identifies factors involved in S. aureus-induced human neutrophil cell death and pathogenesis. Frontiers in Immunology, 10. View this article in WRRO
- Identification of Staphylococcus aureus Factors Required for Pathogenicity and Growth in Human Blood. Infection and immunity, 85(11). View this article in WRRO
- NR4A orphan nuclear receptor family members, NR4A2 and NR4A3, regulate neutrophil number and survival. Blood, 130, 1014-1025. View this article in WRRO
- Ticagrelor potentiates adenosine-induced stimulation of neutrophil chemotaxis and phagocytosis. Vascular Pharmacology, 71, 201-207. View this article in WRRO
- A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism.. Sci Transl Med, 6(225), 225ra29. View this article in WRRO
- Serum and glucocorticoid-regulated kinase 1 regulates neutrophil clearance during inflammation resolution.. J Immunol, 192(4), 1796-1805.
- Muckle-Wells syndrome without mutation in exon 3 of the NLRP3 gene, identified by evidence of excessive monocyte production of functional interleukin 1β and rapid response to anakinra. Clinical and Experimental Dermatology.
- Roles of neutrophils in the regulation of the extent of human inflammation through delivery of IL-1 and clearance of chemokines.. J Leukoc Biol, 93(1), 7-19. View this article in WRRO
- Pellino-1 selectively regulates epithelial cell responses to rhinovirus.. J Virol, 86(12), 6595-6604.
- Staphylococcus aureus induces eosinophil cell death mediated by α-hemolysin.. PLoS One, 7(2), e31506. View this article in WRRO
- The role of TLRs in neutrophil activation. CURR OPIN PHARMACOL, 11(4), 397-403. View this article in WRRO
- Investigating The Role Of Pellino1, An E3 Ubiquitin Ligase, In Modulating Signalling Pathways Controlling The Inflammatory Response. B31. COMPLICATING INFECTIONS: INFLUENZA AND RHINOVIRUS.
- Prosurvival activity for airway neutrophils in severe asthma. THORAX, 65(8), 684-689.
- The rise and rise of Staphylococcus aureus: laughing in the face of granulocytes.. Clin Exp Immunol, 157(2), 216-224.
- Pathways regulating lipopolysaccharide-induced neutrophil survival revealed by lentiviral transduction of primary human neutrophils.. Immunology, 127(2), 249-255.
- The generation of highly purified primary human neutrophils and assessment of apoptosis in response to Toll-like receptor ligands.. Methods Mol Biol, 517, 191-204.
- Ex vivo-expanded bone marrow CD34+ derived neutrophils have limited bactericidal ability.. Stem Cells, 26(10), 2552-2563.
- Subversion of a lysosomal pathway regulating neutrophil apoptosis by a major bacterial toxin, pyocyanin.. J Immunol, 180(5), 3502-3511. View this article in WRRO
- Impairment of apoptotic cell engulfment by pyocyanin, a toxic metabolite of Pseudomonas aeruginosa.. Am J Respir Crit Care Med, 177(1), 35-43. View this article in WRRO
- Inhibition of neutrophil apoptosis by ATP is mediated by the P2Y11 receptor.. J Immunol, 179(12), 8544-8553.
- Translational mini-review series on Toll-like receptors: networks regulated by Toll-like receptors mediate innate and adaptive immunity. Clin Exp Immunol, 147, 199-207.
- Translational mini-review series on Toll-like receptors: networks regulated by Toll-like receptors mediate innate and adaptive immunity.. Clin Exp Immunol, 147(2), 199-207.
- Endotoxin tolerance induces selective alterations in neutrophil function.. J Leukoc Biol, 78(6), 1301-1305.
- The role of interleukin-1beta in direct and toll-like receptor 4-mediated neutrophil activation and survival.. Am J Pathol, 165(5), 1819-1826.
- What can we learn from highly purified neutrophils?. Biochem Soc Trans, 32(Pt3), 468-469.
- Alveolar macrophage apoptosis contributes to pneumococcal clearance in a resolving model of pulmonary infection.. J Immunol, 171(10), 5380-5388. View this article in WRRO
- The role of neutrophil apoptosis in the resolution of acute lung injury in newborn infants. THORAX, 58(11), 961-967.
- Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span.. J Immunol, 170(10), 5268-5275.
- Induction of Neutrophil Apoptosis by the Pseudomonas aeruginosa Exotoxin Pyocyanin: A Potential Mechanism of Persistent Infection. Journal of Immunology, 168(4), 1861-1868. View this article in WRRO
- The CXCL12/CXCR4 signalling axis retains neutrophils at inflammatory sites in zebrafish.
- Macrophage Phenotype Is Associated with Disease Severity in Preterm Infants with Chronic Lung Disease. PLoS ONE, 9(8), e103059-e103059. View this article in WRRO
- A Bittersweet Response to Infection in Diabetes; Targeting Neutrophils to Modify Inflammation and Improve Host Immunity. Frontiers in Immunology, 12.
Chapters
- Pathogen Recognition Receptors and the Lung, Reference Module in Biomedical Sciences Elsevier
Conference proceedings papers
- Hypoglycaemia increases platelet reactivity and promotes formation of proatherogenic platelet-leucocyte aggregates. DIABETOLOGIA, Vol. 60 (pp S532-S532)
- Effect of Hypoglycemia on Platelet Reactivity and Platelet-Leukocyte Kinetics. DIABETES, Vol. 66 (pp A41-A41)
- Hypoglycemia Increases Circulating Proatherogenic Intermediate Monocytes in Healthy Volunteers. DIABETES, Vol. 65 (pp A104-A104)
- TICAGRELOR POTENTIATES NEUTROPHIL PHAGOCYTOSIS VIA INHIBITION OF ADENOSINE REUPTAKE BY ERYTHROCYTES. Journal of the American College of Cardiology, Vol. 65(10) (pp A259-A259)
- The role of neutrophils as anti-inflammatory mediators of airway inflammation. CLINICAL AND EXPERIMENTAL ALLERGY, Vol. 41(12) (pp 1830-1830)
- INVESTIGATING THE ROLE OF PELLIN01, AN E3 UBIQUITIN LIGASE, IN MODULATING SIGNALLING PATHWAYS CONTROLLING THE INFLAMMATORY RESPONSE. THORAX, Vol. 66 (pp A60-A61)
- DIESEL EXHAUST PARTICLES ALTER MONOCYTE DIFFERENTIATION IN VITRO BUT HAVE LITTLE IMPACT ON NEUTROPHIL FUNCTION. THORAX, Vol. 65 (pp A66-A67)
- Subversion of neutrophil apoptosis by Staphylococcus aureus. THORAX, Vol. 62 (pp A114-A115)
- Lentivirus: A tool for the study of neutrophil biology. JOURNAL OF LEUKOCYTE BIOLOGY (pp 36-36)
- A Pseudomonas aeruginosa exotoxin, pyocyanin, impairs phagocytosis and clearance of apoptotic cells. JOURNAL OF LEUKOCYTE BIOLOGY (pp 51-51)
- The Pseudomonas aeruginosa exotoxin pyocyanin induces cathepsin D-mediated neutrophil apoptosis. JOURNAL OF LEUKOCYTE BIOLOGY (pp 43-43)
- The role of toll-like receptors in the regulation of neutrophilic lung inflammation. EUROPEAN RESPIRATORY JOURNAL, Vol. 22 (pp 48S-49S)
- Effect of Hypoglycemia on Inflammatory Responses and the Response to Low Dose Endotoxemia in Humans.. Journal of Clinical Endocrinology and Metabolism, 104(4), 1187-1199. View this article in WRRO
- Professional activities
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- Deputy Chair of School Graduate Research Committee.
- Post-Graduate Year Tutor for the Medical School and Staff-Student Liaison Committee member.
- I teach on the MBChB Phase 2a course.
- Member of the Society for Leukocyte Biology.