Dr Lynne Prince
School of Medicine and Population Health
Senior Lecturer in Innate Immunity
Departmental Director of One University.
l.r.prince@sheffield.ac.uk
+44 114 215 9546
+44 114 215 9546
+44 114 271 1863
(Fax)
The Medical School
Full contact details
Dr Lynne Prince
School of Medicine and Population Health
The Medical School
Beech Hill Road
Sheffield
S10 2RX
School of Medicine and Population Health
The Medical School
Beech Hill Road
Sheffield
S10 2RX
- Profile
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For enquiries please contact - SMPH-West-Operational@sheffield.ac.uk
I graduated from Newcastle University in 1997 with a degree in Medical Microbiology and relocated to Sheffield to study for an MSc in Pathological Sciences (University of Sheffield/Sheffield Hallam University, 1999). This was followed by my PhD entitled `The Regulation of Neutrophil Apoptosis by Bacterial Products´ (University of Sheffield, 2000-04) under the supervision of Professor Moira Whyte. In 2004 I was awarded a University of Sheffield Russell Research Fellowship to study the molecular mechanisms of neutrophil activation and apoptosis.
I was appointed to Senior Lecturer in Innate Immunity in 2022.
- Research interests
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For enquiries, please contact – ClinMed-Operational@sheffield.ac.uk
Inflammation and inflammatory diseases such as chronic obstructive pulmonary disease or rheumatoid arthritis are extremely difficult to treat effectively. During inflammation, inflammatory cells, in particular neutrophils and monocytes, are thought to be key mediators of both tissue damage and perpetuation of further inflammatory signalling. My research focuses on the role of neutrophils during infection and inflammation and how their inflammatory potential and function is regulated by cell death programme, in particular, apoptosis and NETosis. We are currently studying whether an orally active protein kinase C beta inhibitor (ruboxistaurin) and a clinical EGFR/ErbB inhibitor (neratinib) have therapeutic potential in lung and cardiovascular diseases.
My group also studies how the neutrophilic immune response is subverted by pathogens such as Staphylococcus aureus via the Florey Institute for host-pathogen interactions.
My research has been supported by the British Lung Foundation, the Medical Research Council, the Rosetrees Trust, Asthma UK, Dunhill Medical Trust and The University of Sheffield (including the HELSI Institute).
- Publications
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Show: Featured publications All publications
Featured publications
Journal articles
- Effect of Hypoglycemia on Inflammatory Responses and the Response to Low Dose Endotoxemia in Humans.. Journal of Clinical Endocrinology and Metabolism, 104(4), 1187-1199. View this article in WRRO
- Identification of Staphylococcus aureus Factors Required for Pathogenicity and Growth in Human Blood. Infection and immunity, 85(11). View this article in WRRO
- NR4A orphan nuclear receptor family members, NR4A2 and NR4A3, regulate neutrophil number and survival. Blood, 130(8), 1014-1025. View this article in WRRO
- Macrophage phenotype is associated with disease severity in preterm infants with chronic lung disease. PLoS ONE, 9(8). View this article in WRRO
- A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism.. Sci Transl Med, 6(225), 225ra29. View this article in WRRO
- Roles of neutrophils in the regulation of the extent of human inflammation through delivery of IL-1 and clearance of chemokines.. J Leukoc Biol, 93(1), 7-19. View this article in WRRO
- Pellino-1 selectively regulates epithelial cell responses to rhinovirus.. J Virol, 86(12), 6595-6604.
- Staphylococcus aureus induces eosinophil cell death mediated by α-hemolysin.. PLoS One, 7(2), e31506. View this article in WRRO
- Pathways regulating lipopolysaccharide-induced neutrophil survival revealed by lentiviral transduction of primary human neutrophils.. Immunology, 127(2), 249-255.
- Ex vivo-expanded bone marrow CD34+ derived neutrophils have limited bactericidal ability.. Stem Cells, 26(10), 2552-2563.
- Subversion of a lysosomal pathway regulating neutrophil apoptosis by a major bacterial toxin, pyocyanin.. J Immunol, 180(5), 3502-3511. View this article in WRRO
- Impairment of apoptotic cell engulfment by pyocyanin, a toxic metabolite of Pseudomonas aeruginosa.. Am J Respir Crit Care Med, 177(1), 35-43. View this article in WRRO
- The role of interleukin-1beta in direct and toll-like receptor 4-mediated neutrophil activation and survival.. Am J Pathol, 165(5), 1819-1826.
- The role of neutrophil apoptosis in the resolution of acute lung injury in newborn infants. THORAX, 58(11), 961-967.
- Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span.. J Immunol, 170(10), 5268-5275.
- Induction of Neutrophil Apoptosis by the Pseudomonas aeruginosa Exotoxin Pyocyanin: A Potential Mechanism of Persistent Infection. Journal of Immunology, 168(4), 1861-1868. View this article in WRRO
All publications
Journal articles
- Barriers to equality, diversity and inclusion in research and academia stubbornly persist. So, what are we doing about it?. Disease Models & Mechanisms, 16(7).
- The EGFR/ErbB inhibitor neratinib modifies the neutrophil phosphoproteome and promotes apoptosis and clearance by airway macrophages. Frontiers in Immunology, 13. View this article in WRRO
- Enhanced neutrophil extracellular trap formation in COVID-19 is inhibited by the PKC inhibitor ruboxistaurin. ERJ Open Research. View this article in WRRO
- A bittersweet response to infection in diabetes ; targeting neutrophils to modify inflammation and improve host immunity. Frontiers in Immunology, 12. View this article in WRRO
- The epidermal growth factor ligand Spitz modulates macrophage efferocytosis, wound responses and migration dynamics during Drosophila embryogenesis. Frontiers in Cell and Developmental Biology, 9. View this article in WRRO
- Isolation and high throughput flow cytometric apoptosis assay of human neutrophils to enable compound library screening. Bio-protocol, 10(11). View this article in WRRO
- Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation. eLife, 8, ---. View this article in WRRO
- Pellino-1 regulates immune responses to Haemophilus influenzae in models of inflammatory lung disease. Frontiers in Immunology, 10. View this article in WRRO
- The CXCL12/CXCR4 signaling axis retains neutrophils at inflammatory sites in zebrafish. Frontiers in Immunology, 10. View this article in WRRO
- Effect of Hypoglycemia on Inflammatory Responses and the Response to Low Dose Endotoxemia in Humans.. Journal of Clinical Endocrinology and Metabolism, 104(4), 1187-1199. View this article in WRRO
- A genome-wide screen identifies factors involved in S. aureus-induced human neutrophil cell death and pathogenesis. Frontiers in Immunology, 10. View this article in WRRO
- Identification of Staphylococcus aureus Factors Required for Pathogenicity and Growth in Human Blood. Infection and immunity, 85(11). View this article in WRRO
- NR4A orphan nuclear receptor family members, NR4A2 and NR4A3, regulate neutrophil number and survival. Blood, 130(8), 1014-1025. View this article in WRRO
- Ticagrelor potentiates adenosine-induced stimulation of neutrophil chemotaxis and phagocytosis. Vascular Pharmacology, 71, 201-207. View this article in WRRO
- Macrophage phenotype is associated with disease severity in preterm infants with chronic lung disease. PLoS ONE, 9(8). View this article in WRRO
- A zebrafish compound screen reveals modulation of neutrophil reverse migration as an anti-inflammatory mechanism.. Sci Transl Med, 6(225), 225ra29. View this article in WRRO
- Serum and glucocorticoid-regulated kinase 1 regulates neutrophil clearance during inflammation resolution.. J Immunol, 192(4), 1796-1805.
- Muckle-Wells syndrome without mutation in exon 3 of the NLRP3 gene, identified by evidence of excessive monocyte production of functional interleukin 1β and rapid response to anakinra. Clinical and Experimental Dermatology.
- Roles of neutrophils in the regulation of the extent of human inflammation through delivery of IL-1 and clearance of chemokines.. J Leukoc Biol, 93(1), 7-19. View this article in WRRO
- Pellino-1 selectively regulates epithelial cell responses to rhinovirus.. J Virol, 86(12), 6595-6604.
- Staphylococcus aureus induces eosinophil cell death mediated by α-hemolysin.. PLoS One, 7(2), e31506. View this article in WRRO
- The role of TLRs in neutrophil activation. CURR OPIN PHARMACOL, 11(4), 397-403. View this article in WRRO
- Investigating The Role Of Pellino1, An E3 Ubiquitin Ligase, In Modulating Signalling Pathways Controlling The Inflammatory Response. B31. COMPLICATING INFECTIONS: INFLUENZA AND RHINOVIRUS.
- Prosurvival activity for airway neutrophils in severe asthma. THORAX, 65(8), 684-689.
- The rise and rise of Staphylococcus aureus: laughing in the face of granulocytes.. Clin Exp Immunol, 157(2), 216-224.
- Pathways regulating lipopolysaccharide-induced neutrophil survival revealed by lentiviral transduction of primary human neutrophils.. Immunology, 127(2), 249-255.
- The generation of highly purified primary human neutrophils and assessment of apoptosis in response to Toll-like receptor ligands.. Methods Mol Biol, 517, 191-204.
- Ex vivo-expanded bone marrow CD34+ derived neutrophils have limited bactericidal ability.. Stem Cells, 26(10), 2552-2563.
- Subversion of a lysosomal pathway regulating neutrophil apoptosis by a major bacterial toxin, pyocyanin.. J Immunol, 180(5), 3502-3511. View this article in WRRO
- Impairment of apoptotic cell engulfment by pyocyanin, a toxic metabolite of Pseudomonas aeruginosa.. Am J Respir Crit Care Med, 177(1), 35-43. View this article in WRRO
- Inhibition of neutrophil apoptosis by ATP is mediated by the P2Y11 receptor.. J Immunol, 179(12), 8544-8553.
- Translational mini-review series on Toll-like receptors: networks regulated by Toll-like receptors mediate innate and adaptive immunity. Clin Exp Immunol, 147, 199-207.
- Translational mini-review series on Toll-like receptors: networks regulated by Toll-like receptors mediate innate and adaptive immunity.. Clin Exp Immunol, 147(2), 199-207.
- Endotoxin tolerance induces selective alterations in neutrophil function.. J Leukoc Biol, 78(6), 1301-1305.
- The role of interleukin-1beta in direct and toll-like receptor 4-mediated neutrophil activation and survival.. Am J Pathol, 165(5), 1819-1826.
- What can we learn from highly purified neutrophils?. Biochem Soc Trans, 32(Pt3), 468-469.
- Alveolar macrophage apoptosis contributes to pneumococcal clearance in a resolving model of pulmonary infection.. J Immunol, 171(10), 5380-5388. View this article in WRRO
- The role of neutrophil apoptosis in the resolution of acute lung injury in newborn infants. THORAX, 58(11), 961-967.
- Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span.. J Immunol, 170(10), 5268-5275.
- Toll-Like Receptor (TLR)2 and TLR4 in Human Peripheral Blood Granulocytes: A Critical Role for Monocytes in Leukocyte Lipopolysaccharide Responses. The Journal of Immunology, 168(9), 4701-4710.
- Induction of Neutrophil Apoptosis by the Pseudomonas aeruginosa Exotoxin Pyocyanin: A Potential Mechanism of Persistent Infection. Journal of Immunology, 168(4), 1861-1868. View this article in WRRO
- Inflammatory neutrophils retain susceptibility to apoptosis mediated via the Fas death receptor. Journal of Leukocyte Biology, 67(5), 662-668.
Chapters
- Pathogen Recognition Receptors and the Lung, Reference Module in Biomedical Sciences Elsevier
Conference proceedings papers
- Hypoglycaemia increases platelet reactivity and promotes formation of proatherogenic platelet-leucocyte aggregates. DIABETOLOGIA, Vol. 60 (pp S532-S532)
- Effect of Hypoglycemia on Platelet Reactivity and Platelet-Leukocyte Kinetics. DIABETES, Vol. 66 (pp A41-A41)
- Hypoglycemia Increases Circulating Proatherogenic Intermediate Monocytes in Healthy Volunteers. DIABETES, Vol. 65 (pp A104-A104)
- TICAGRELOR POTENTIATES NEUTROPHIL PHAGOCYTOSIS VIA INHIBITION OF ADENOSINE REUPTAKE BY ERYTHROCYTES. Journal of the American College of Cardiology, Vol. 65(10) (pp A259-A259)
- INVESTIGATING THE ROLE OF PELLIN01, AN E3 UBIQUITIN LIGASE, IN MODULATING SIGNALLING PATHWAYS CONTROLLING THE INFLAMMATORY RESPONSE. THORAX, Vol. 66 (pp A60-A61)
- The role of neutrophils as anti-inflammatory mediators of airway inflammation. CLINICAL AND EXPERIMENTAL ALLERGY, Vol. 41(12) (pp 1830-1830)
- DIESEL EXHAUST PARTICLES ALTER MONOCYTE DIFFERENTIATION IN VITRO BUT HAVE LITTLE IMPACT ON NEUTROPHIL FUNCTION. THORAX, Vol. 65 (pp A66-A67)
- Subversion of neutrophil apoptosis by Staphylococcus aureus. THORAX, Vol. 62 (pp A114-A115)
- Lentivirus: A tool for the study of neutrophil biology. JOURNAL OF LEUKOCYTE BIOLOGY (pp 36-36)
- A Pseudomonas aeruginosa exotoxin, pyocyanin, impairs phagocytosis and clearance of apoptotic cells. JOURNAL OF LEUKOCYTE BIOLOGY (pp 51-51)
- The Pseudomonas aeruginosa exotoxin pyocyanin induces cathepsin D-mediated neutrophil apoptosis. JOURNAL OF LEUKOCYTE BIOLOGY (pp 43-43)
- The role of toll-like receptors in the regulation of neutrophilic lung inflammation. EUROPEAN RESPIRATORY JOURNAL, Vol. 22 (pp 48S-49S)
Preprints
- Pulses of Class I PI3kinase activity identify the release and recapture of prey from neutrophil phagosomes, Cold Spring Harbor Laboratory.
- Determining the importance of the stringent response for methicillin-resistantStaphylococcus aureusvirulence using a zebrafish model of infection, Cold Spring Harbor Laboratory.
- Multi-modal imaging reveals dynamic interactions of Staphylococcus aureus within human neutrophils, Cold Spring Harbor Laboratory.
- The EGFR/ErbB inhibitor neratinib modifies the neutrophil phosphoproteome and promotes apoptosis and clearance by airway macrophages, Cold Spring Harbor Laboratory.
- Enhanced neutrophil extracellular trap formation in COVID-19 is inhibited by the PKC inhibitor ruboxistaurin, Cold Spring Harbor Laboratory.
- The CXCL12/CXCR4 signalling axis retains neutrophils at inflammatory sites in zebrafish.
- Effect of Hypoglycemia on Inflammatory Responses and the Response to Low Dose Endotoxemia in Humans.. Journal of Clinical Endocrinology and Metabolism, 104(4), 1187-1199. View this article in WRRO
- Teaching activities
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- MSc module lead (Molecular Medicine): Model Systems in Research.
- Deputy module lead (BMS): Pharmacology of Respiratory Disease
- MBChB teaching: I facilitate the ILA programme for phase 1 and give a Respiratory pharmacology lecture for phase 2a.
- Faculty postgraduate induction course (PIC)
- Professional activities and memberships
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Deputy Faculty PGR Director (Progression).
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Member of the Faculty Graduate Research Committee (FGRC).
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Deputy Department Theme Lead.
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Member of the Society for Leukocyte Biology.
Previous roles: Chair of Medical School Graduate Research Committee (SGRC), Medical School Lead for Postgraduate Researcher Development, Medical School PGR Year Tutor, Co-Deputy Dept PGR Lead, Dept admissions tutor.
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