Biomedical Materials

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OUR EQUIPMENT:

Mauna Kea Technologies Cellvizio Dual Band
Zeiss Lightsheet Z.1

MAUNA KEA TECHNOLOGIES CELLVIZIO DUAL BAND

3D cellular and sub-cellular imaging of tissue. 


DETAILED DESCRIPTION

This equipment is a fluorescent-based Confocal Laser Endomicroscopy (CLE) system, bringing the confocal microprobe in contact with the tissue of interest to record realtime images of the tissue at cellular resolution, in vivo. The dual-colour capability (488nm and 660nm excitations) allows the capture of functional and morphological images of molecular mechanisms in real time. 


DETAILED SPECIFICATIONS

Simultaneous 488nm and 660nm. First line 502 - 633nm. Second line 673 - 800 nm. 

Resolution: Between 3.3 and 1.4 μm 
Frame rate: 9-18 fps
Advanced mode: 40 fps
Image: png, bmp, jpeg, tiff, mhd
Video: avi, mpeg, mp4, swf
Linux Ubuntu
Laser class: 3R
Height: 310mm
Width: 480mm
Depth: 525mm
Weight: 35kg
Cellvizio Lab Software 2.1.0.


LOCATION
Kroto Research Institute, Red Hill, Sheffield City Centre, Sheffield, S3 7HQ

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ZEISS LIGHTSHEET Z.1

This equipment is used to image relatively large, living samples that are fluorescently labelled in some way. 


DETAILED DESCRIPTION

The equipment has incubation capabilities, so can be used with live samples (note that due to licensing limitations, this equipment cannot be used for imaging living organisms). There are five laser lines available with this instrument (405nm, 488nm, 514nm, 561nm, and 638nm) and the equipment is suitable for imaging whole samples up to approx 200 μm thick. 

The Lightsheet comes with three detection objectives (5x, 10x, and 20x) along with a zoom facility which allows between 0.3x and 2.5x magnification. As a result the equipment can achieve magnifications between 1.5x and 50x. 


LOCATION
Kroto Research Institute, Red Hill, Sheffield City Centre, Sheffield, S3 7HQ

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ENQUIRE HERE: royce@sheffield.ac.uk


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