Flow cytometry core service
The Medical School's flow cytometry facility is a core facility based in the RHH Medical School and provides a service to all at the University and externally.
Flow cytometry is a technique that enables single whole particles/cells to be analysed. Events pass through lasers and physical properties such as relative size (FSC) and granularity/internal complexity (SSC) are detected. If there is any fluorescence also associated with the cell this can also be seen, with the emitted light being diverted via a series of mirrors and filters and collected by PMTs with specific bandpass filters in front of them.
Phycoerthrin (PE) - Excitation Max 561 nm
- Emission Max 576nm
- Detection Band pass filter 575/26nm (ie 562-588nm)
Using physical or fluorescent properties, cells can be separated from a parent population into different tubes, this is called cell sorting. Cells can be bulk sorted into tubes, or single cells can be sorted into 96/384 well plates.
- Immunophenotyping (up to 40 colours on Cytek Aurora 5 laser)
- Cell cycle
- Calcium flux
- Cell counts
- Detection of Extracellular vesicles, bacteria, algae
- Transfection efficiency (e,g GFP, mCherry)
The facility comprises
- 2 Cytek Aurora spectral analysers
- 1 LSRII analyser
- 1 FACSMelody cell sorter
- A soluble cytokine quantification service
- Analysis software FCSExpress (available from August 2023)
Unlike the traditional flow cytometers which detect only a small portion of a fluorochromes spectral emission, the spectral analysers collect all the light emitted. This enables multiple fluorochromes to be separated and analysed simultaneously.
In May 2023 we took delivery of a second Cytek Aurora. This is a five-laser system with a plate loader capable of detecting up to 40 different colours.
Our staff can help you with all aspects of your work including planning and running experiments and data analysis.
Technical time can be booked on the Flow Google Calendar and all the machines can be booked through the Flow Lab on Clustermarket. Please contact the facility for access.
Contact us at email@example.com.
LSRII: £45 per hour
Aurora 3-laser or 5-laser: £55 per hour
FACSMelody - £80
UV laser 355nm
Violet laser 405nm
Blue laser 488nm
Red laser 633nm
Blue laser 488nm
Red laser 640nm
- Aurora (3-laser)
16 channels (420-829nm)
14 channels (498-829nm)
8 channels (652-829nm)
- Aurora (5-laser)
10 channels 365-829nm
16 channels 420-829nm
14 channels 498-829nm
10 channels 567-829nm
8 channels 652-829nm
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