Dr Jagroop Pandhal
BSc (Hons), MSc (Hons), PhD
Department of Chemical and Biological Engineering
Senior Lecturer in Biological Engineering
+44 114 222 4914
Full contact details
Department of Chemical and Biological Engineering
Sir Robert Hadfield Building
I am a biological engineer with a passion for exploiting the diverse functional capabilities of microbes to answer emerging societal challenges. We are largely focussed on metabolic engineering of microbial systems for making all sorts of products from high to medium/low value, including therapeutic proteins and polyols for bio-plastics. We also use microbes for bioremediation and resource recovery, including toxic industrial waste streams all the way to chemical warfare agents. More recently we have become fascinated with the concept of creating synthetic microbial communities to face some of the more complex challenges. We apply a variety of tools to uncover interactions and metabolic functions, with quantitative proteomics being one of our favourite tools.
My undergraduate was in Microbiology in the Department of Molecular Biology and Biotechnology at The University of Sheffield. I then spent time doing research in Chicago, USA followed a year of research in applied aspects of biosciences in Nottingham. However, I returned to Sheffield for my doctoral studies and worked at the interface of life sciences and engineering, developing quantitative proteomics tools to complement environment-focused projects with functional characterisation. After a successful PDRA funded by the Bioprocess Research Industry Club I was awarded an Independent Fellowship from NERC. Since 2015, I have led my own research team as an academic in the Department of Chemical and Biological Engineering (CBE). Although this means I have spent a long time in Sheffield, I have made an excellent base to collaborate with academics across all faculties, and have some brilliant friends to share ideas and write grants with.
I am particularly proud of my research group, and so far, have been lucky to supervise an incredibly international team of PhD students and PDRA’s with representatives from Portugal, Italy, India, Cameroon, Iraq, UK, Kuwait, Nigeria, Malaysia, China, Brazil and Mexico. My other role in the department is PGR Admissions Tutor, and having undertaken my PhD here many, many years ago, I feel well placed to share the merits of being part of CBE.
- Research interests
My Research interests are:
- Metabolic engineering
- Quantitative proteomics (metaproteomics and glycosylation)
- Synthetic microbial communities
- Algae Biotechnology
It is widely recognised that the fundamental training of a biologist and an engineer is different. Mathematical theories and quantitative methods are at the forefront of engineering approaches, and therefore their application to complex systems, including biological, is a useful attribute.
However, biologists have the advantage of formulating better testable hypotheses, experimental designs and data interpretation from these complex biological systems. This is namely due to different techniques and strategies used by life scientists to qualitatively decipher complex systems.
The skills of an engineer and life scientist are therefore complementary. I work at this interface to reveal (hopefully useful) information about complex biological systems.
- Metabolic insights into infochemicals induced colony formation and flocculation in scenedesmus subspicatus unraveled by quantitative proteomics. Frontiers in Microbiology, 11.
- Structural equation modelling reveals that nutrients and physicochemistry act additively on the dynamics of a microcosm-based biotic community. Biology, 8(4). View this article in WRRO
- Engineering Pathways in Central Carbon Metabolism Help to Increase Glycan Production and Improve N-Type Glycosylation of Recombinant Proteins in E. coli. BioEngineering, 6(1). View this article in WRRO
- Circular economy fertilization: Testing micro and macro algal species as soil improvers and nutrient sources for crop production in greenhouse and field conditions. Geoderma, 334, 113-123. View this article in WRRO
- Metaproteomics of Freshwater Microbial Communities, 145-155.
- The Effect of High-Intensity Ultraviolet Light to Elicit Microalgal Cell Lysis and Enhance Lipid Extraction.. Metabolites, 8(4). View this article in WRRO
- Cell lysis and detoxification of cyanotoxins using a novel combination of microbubble generation and plasma microreactor technology for ozonation. Frontiers in Microbiology, 9. View this article in WRRO
- Harvesting environmental microalgal blooms for remediation and resource recovery: A laboratory scale investigation with economic and microbial community impact assessment. Biology, 7(1), 4-4. View this article in WRRO
- Biotechnological conversion of methane to methanol: evaluation of progress and potential. AIMS Bioengineering, 5(1), 1-38. View this article in WRRO
- Quantitative proteomics of a B12‐dependent alga grown in coculture with bacteria reveals metabolic tradeoffs required for mutualism. New Phytologist, 217(2), 599-612. View this article in WRRO
- Competitive growth experiments with a high-lipid Chlamydomonas reinhardtii mutant strain and its wild-type to predict industrial and ecological risks. AMB Express, 7. View this article in WRRO
- Producing a glycosylating Escherichia coli cell factory: The placement of the bacterial oligosaccharyl transferase pglB onto the genome. Biochemical and Biophysical Research Communications. View this article in WRRO
- Generation of Recombinant N-Linked Glycoproteins in E. coli. Methods in molecular biology (Clifton, N.J.), 233-250. View this article in WRRO
- Erratum to: The use of natural infochemicals for sustainable and efficient harvesting of the microalgae Scenedesmus spp. for biotechnology: insights from a meta-analysis. Biotechnology Letters.
- The use of natural infochemicals for sustainable and efficient harvesting of the microalgae Scenedesmus spp. for biotechnology: insights from a meta-analysis.. Biotechnol Lett. View this article in WRRO
- A metaproteomic analysis of the response of a freshwater microbial community under nutrient enrichment.. Frontiers in Microbiology, 7, 1172-1172. View this article in WRRO
- Quantitative definition and monitoring of the host cell protein proteome using iTRAQ - a study of an industrial mAb producing CHO-S cell line. Biotechnology Journal, 11(8), 1014-1024. View this article in WRRO
- Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli, 17-35.
- Escherichia coli as a glycoprotein production host: recent developments and challenges. Current Opinion in Biotechnology, 30, 205-210.
- Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species.. Biochem J, 458(3), 499-511. View this article in WRRO
- Synthetic microbial ecosystems for biotechnology.. Biotechnol Lett, 36(6), 1141-1151.
- Monitoring earths's canary. Planet Earth(WINTER), 22-24.
- Expanding the bioprocessing toolbox ofEscherichia colithrough metabolic engineering and synthetic biology: an emerging glycosylation chassis. Pharmaceutical Bioprocessing, 1(3), 221-224.
- Inverse metabolic engineering to improve Escherichia coli as an N-glycosylation host.. Biotechnol Bioeng, 110(9), 2482-2493.
- Genome-scale identification and characterization of ethanol tolerance genes in Escherichia coli.. Metab Eng, 15, 124-133.
- HILIC- and SCX-based quantitative proteomics of Chlamydomonas reinhardtii during nitrogen starvation induced lipid and carbohydrate accumulation.. J Proteome Res, 11(12), 5959-5971.
- An insight into iTRAQ: where do we stand now?. Anal Bioanal Chem, 404(4), 1011-1027.
- Systematic metabolic engineering for improvement of glycosylation efficiency in Escherichia coli.. Biochem Biophys Res Commun, 419(3), 472-476.
- Methods in quantitative proteomics: Setting iTRAQ on the right track. Current Proteomics, 8(1), 17-30.
- Improving N-glycosylation efficiency in Escherichia coli using shotgun proteomics, metabolic network analysis, and selective reaction monitoring.. Biotechnol Bioeng, 108(4), 902-912.
- N-Linked glycoengineering for human therapeutic proteins in bacteria.. Biotechnol Lett, 32(9), 1189-1198.
- Exploiting cyanobacterial P450 pathways.. Curr Opin Microbiol, 13(3), 301-306.
- Exploiting cyanobacterial P450 pathways. Current Opinion in Microbiology, 13(3), 301-306.
- Comparative proteomics study of salt tolerance between a nonsequenced extremely halotolerant cyanobacterium and its mildly halotolerant relative using in vivo metabolic labeling and in vitro isobaric labeling.. J Proteome Res, 8(2), 818-828.
- A systems biology approach to investigate the response of Synechocystis sp. PCC6803 to a high salt environment.. Saline Systems, 5, 8. View this article in WRRO
- Isolation and characterisation of microsatellite loci in the papillose woolly bat, Kerivoula papillosa (Chiroptera : Vespertilionidae). CONSERV GENET, 9(3), 751-756.
- A cross-species quantitative proteomic study of salt adaptation in a halotolerant environmental isolate using 15N metabolic labelling.. Proteomics, 8(11), 2266-2284.
- Proteomics with a pinch of salt: a cyanobacterial perspective.. Saline Systems, 4, 1. View this article in WRRO
- Isolation of 22 new Haliaeetus microsatellite loci and their characterization in the critically endangered Madagascar fish-eagle (Haliaeetus vociferoides) and three other Haliaeetus eagle species. MOL ECOL NOTES, 7(4), 711-715.
- A quantitative proteomic analysis of light adaptation in a globally significant marine cyanobacterium Prochlorococcus marinus MED4.. J Proteome Res, 6(3), 996-1005.
- A predicted microsatellite map of the passerine genome based on chicken-passerine sequence similarity.. Mol Ecol, 15(5), 1299-1320.
- Assessment of 17 new whiskered auklet (Aethia pygmaea) microsatellite loci in 42 seabirds identifies 5-15 polymorphic markers for each of nine Alcinae species. MOL ECOL NOTES, 5(2), 289-297.
- Isolation and characterization of microsatellite loci from two inbreeding bark beetle species (Coccotrypes). MOL ECOL NOTES, 3(2), 270-273. View this article in WRRO
- Isolation of 22 new Haliaeetus microsatellite loci and their characterization in the critically endangered Madagascar fish-eagle (Haliaeetus vociferoides) and three other Haliaeetus eagle species. Molecular Ecology Notes, 0(0), 070621074211164-???.
- Designing synthetic bacterial consortia for landfill leachate treatment based on community matrices and regression tree analysis.
- Microwave-Assisted Extraction for Microalgae: From Biofuels to Biorefinery. Biology, 7(1). View this article in WRRO
- CHAPTER 3. Making Sense Out of the Proteome: the Utility of iTRAQ and TMT, Quantitative Proteomics (pp. 49-79). Royal Society of Chemistry
Conference proceedings papers
- Plasma Reactor Characterization of Lignocellulosic Materials on Agricultural Waste Treatment. Advanced Science Letters, Vol. 24(12) (pp 9502-9504)
- Application of the Microbubble Enhanced Ozonolysis Technology in Biomass Utilisation. Fourth International Conference on Advances in Bio-Informatics, Bio-Technology and Environmental Engineering - ABBE 2016
- Optimisation of Protein Extraction Methods for Metaproteomics of Freshwater Microbial Communities. USES Conference Proceedings
- Teaching interests
I teach fundamental concepts in biotechnology to first year students through to more complex unit operations involved in environmental engineering to MEng and MSc students. This covers about 350 students.
- Teaching activities
- CPE190 Engineering with Living Systems I
- CPE 408/6081 Environmental Engineering: Liquid Effluents
- MAT308 Research Seminars in Bioengineering