Theory & Practise

Intermediate ImmunohistochemistryIntermediate Immunohistochemistry

17 April 2018 – book now

This course is accredited by the Institute of Biomedical Science and will provide you with six IBMS credits.

This course is the second module in the series of Theory & Practise Immunohistochemistry aimed at those working within the NHS, higher education institutions or within industry. The Intermediate course is a one day theory and practical workshop which aims to provide a more in depth focus on IHC and its applications within your workplace.

On completion of the course, students will understand:

  • What factors need to be considered in order to create a successful IHC method which allows for multiple identification of cellular markers/antigens in a single tissue section.
  • The issues associated with multiple IHC staining.

On completion of the course, students will be able to:

  • Apply a sound approach to the creation of a multiple IHC staining method.
  • Explain why some reactions aren’t compatible and find ways of resolving this.
  • Prepare reagents and antibodies suitable for use in IHC techniques.
  • Be confident in how to react when automation goes wrong.
  • Use a microscope to examine IHC staining.

Intermediate ImmunohistochemistryThe topics covered in the module include:

  • Overview of IHC
  • Use of polymer kits, tyramide amplification, fluorescence for multiple staining
  • How to create a IHC protocol
  • Automation
  • Troubleshooting
  • Microscope analysis

Is this course right for me?

The intermediate IHC course is suitable for anyone who has a basic background in IHC and wishes to gain a better understanding of what’s involved in taking this technique a step further. This one day workshop contains both a theoretical and practical element. Refreshments and lunch will be provided. Delegates are invited to bring their own slides for troubleshooting if desired.

17 April 2018 – book now

SITraN, Department of Neuroscience, The University of Sheffield, Sheffield, S10 2HQ, United Kingdom

The cost of this one day workshop is £180. For more information, contact:

Natalie Kennerley -, 0114 222 6600
Lynne Baxter –, 0114 2222272/51