Theory & Practise
This course is accredited by the Institute of Biomedical Science and will provide you with six IBMS credits.
This course is the second module in the series of Theory & Practise Immunohistochemistry aimed at those working within the NHS, higher education institutions or within industry. The Intermediate course is a one day theory and practical workshop which aims to provide a more in depth focus on IHC and its applications within your workplace.
On completion of the course, students will understand:
- What factors need to be considered in order to create a successful IHC method which allows for multiple identification of cellular markers/antigens in a single tissue section.
- The issues associated with multiple IHC staining.
On completion of the course, students will be able to:
- Apply a sound approach to the creation of a multiple IHC staining method.
- Explain why some reactions aren’t compatible and find ways of resolving this.
- Prepare reagents and antibodies suitable for use in IHC techniques.
- Be confident in how to react when automation goes wrong.
- Use a microscope to examine IHC staining.
The topics covered in the module include:
- Overview of IHC
- Use of polymer kits, tyramide amplification, fluorescence for multiple staining
- How to create a IHC protocol
- Microscope analysis
Is this course right for me?
The intermediate IHC course is suitable for anyone who has a basic background in IHC and wishes to gain a better understanding of what’s involved in taking this technique a step further. This one day workshop contains both a theoretical and practical element. Refreshments and lunch will be provided. Delegates are invited to bring their own slides for troubleshooting if desired.
17 April 2018 – book now
SITraN, Department of Neuroscience, The University of Sheffield, Sheffield, S10 2HQ, United Kingdom
The cost of this one day workshop is £180. For more information, contact: