Dr Thushan de Silva FRCP (Glasg) FRCPath DTM&H PhD
Honorary Senior Clinical Lecturer
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield
Beech Hill Road
Tel: +44 (0)114 215 9532
Fax: +44 (0)114 271 1863
I am a clinician scientist and Wellcome Trust Intermediate Clinical Fellow, currently based at the MRC Unit The Gambia at LSHTM as a Principal Investigator in the Vaccines and Immunity Theme. I am also an Honorary Consultant Physician in Infectious Diseases at Sheffield Teaching Hospital NHS Foundation Trust.
I completed my undergraduate medical training at the University of Bristol in 2000. Following postgraduate clinical training in General Internal Medicine, Infectious Diseases and HIV in Oxford and London, I moved to Sheffield for higher specialist training in Infectious Diseases & Microbiology in 2004. I was awarded a British Infection Society Research Fellowship (2007-8), followed by an MRC Clinical Research Training Fellowship (2008 – 11) under the supervision of Professor Sarah Rowland-Jones (Oxford) and Professor Robin Weiss (UCL) to study the pathogenesis of HIV-2 infection and molecular epidemiology of HIV-1 and HIV-2, based primarily at the MRC Unit The Gambia.
I joined the University of Sheffield in 2013 as an NIHR Academic Clinical Lecturer in Infectious Diseases & Microbiology and was awarded an Academy of Medical Sciences starter grant to undertake pilot studies of live attenuated influenza vaccination (LAIV) in adult volunteers. Data generated during these lead to the successful award of a Wellcome Trust Intermediate Clinical Fellowship in 2016 under the sponsorship of Professor Beate Kampmann (MRC Gambia/Imperial College London) to be undertaken between Imperial College London, MRC Unit The Gambia at LSHTM and the University of Sheffield.
My interests fall into three main areas:
- My primary interest is the study of anti-viral immune responses in the context of both vaccination and natural infection. Building on skills gained during my PhD, which focused on HIV-specific humoral and T-cell correlates of protection from disease progression in HIV-2 infection, the focus of my current research is the detailed mucosal and systemic immunogenicity of live attenuated influenza vaccine (LAIV) in Gambian children using a systems vaccinology approach, including interactions with the host nasopharyngeal microbiome.
- Extending my work on immune responses in immunosuppressed hosts, I am interested in vaccine responses in Haematopoietic Stem Cell Transplant patients, collaborating with Professor John Snowden and Professor Diana Greenfield at STH.
- Aligning with The Florey Institute strategy, I am leading pilot studies characterizing MRSA (Sri Lanka) and Streptococcus pyogenes (The Gambia) isolates via whole genome sequencing.
I am co-organiser of the Sheffield Diploma in Tropical Medicine and Hygiene (DTM&H) course that prepares candidates to sit the Royal College of Physicians DTM&H examination. When based in Sheffield, I am involved in delivering the undergraduate (MBChB) Infectious Diseases, Tropical Medicine and Microbiology curriculum at the University of Sheffield.
- Fellow of the Royal Society of Tropical Medicine and Hygiene.
- Member of the British HIV Association, British Infection Association and the European Society of Clinical Microbiology and Infectious Diseases.
- Member of HIC-Vac network.
- Immunogenicity of intranasal live attenuated influenza vaccination (LAIV) and bidirectional interactions with the host microbiome in Gambian children (Wellcome Trust Intermediate Clinical Fellowship 2016 – 2020).
- Nasopharyngeal virome sequencing to determine the impact of respiratory viral interference with live attenuated influenza vaccine immunogenicity in Gambian children (Global Health Seed Grant, Imperial College London, 2017).
- A study of scabies and group A streptococcal pyoderma in The Gambia (The SPYDERM study) – HEFCE/ODA grant via The University of Sheffield (2018), collaboration with Dr. Claire Turner, MBB.
- Analysis of the immune response to Fendrix (an MPL-adjuvanted vaccine) as compared to double-dose Engerix B in HIV-infected non-responders to standard Hepatitis B vaccination courses (British HIV Association Research Award 2013).
For key publications see below. For a full list of publications click here.
- de Lima DS, Cardozo LE, Maracaja-Coutinho V, Suhrbier A, Mane K, Jeffries D, Silveira ELV, Amaral PP, Rappuoli R, de Silva TI & Nakaya HI (2019) Long noncoding RNAs are involved in multiple immunological pathways in response to vaccination. Proceedings of the National Academy of Sciences, 116(34), 17121-17126.
- Lindsey BB, Jagne YJ, Armitage EP, Singanayagam A, Sallah HJ, Drammeh S, Senghore E, Mohammed NI, Jeffries D, Höschler K , Tregoning JS et al (2019) Effect of a Russian-backbone live-attenuated influenza vaccine with an updated pandemic H1N1 strain on shedding and immunogenicity among children in The Gambia : an open-label, observational, phase 4 study. The Lancet Respiratory Medicine. View this article in WRRO
- McTavish S, Snow S, Cook E, Pichon B, Coleman S, Coombs G, Pang S, Arias C, Diaz L, Boldock E , Davies S et al (2019) Genomic and epidemiological evidence of a dominant Panton-Valentine leucocidin-positive Methicillin Resistant Staphylococcus aureus lineage in Sri Lanka and presence among isolates from the United Kingdom and Australia. Frontiers in Cellular and Infection Microbiology, 9. View this article in WRRO
- Miller PDE, de Silva TI, Leonard H, Anthias C, Hoschler K, Goddard K, Peggs K, Madrigal A & Snowden JA (2019) A comparison of viral microneutralization and haemagglutination inhibition assays as measures of seasonal inactivated influenza vaccine immunogenicity in the first year after reduced intensity conditioning, lymphocyte depleted allogeneic haematopoietic stem cell transplant. Vaccine, 37(3), 452-457. View this article in WRRO
- Lindsey BB, Armitage EP, Kampmann B & de Silva TI (2018) The efficacy, effectiveness, and immunogenicity of influenza vaccines in Africa: a systematic review. The Lancet Infectious Diseases. View this article in WRRO
- de Silva T, Leligdowicz A, Carlson J, Garcia-Knight M, Onyango C, Miller N, Yindom LM, Hue S, Jaye A, Dong T , Cotten M et al (2018) HLA-associated polymorphisms in the HIV-2 capsid highlight key differences between HIV-1 and HIV-2 immune adaptation. AIDS, 6, 704-709. View this article in WRRO
- Armitage EP, Camara J, Bah S, Forster AS, Clarke E, Kampmann B & de Silva TI (2018) Acceptability of intranasal live attenuated influenza vaccine, influenza knowledge and vaccine intent in The Gambia. Vaccine, 36(13), 1772-1780. View this article in WRRO
- de Silva TI, Gould V, Mohammed NI, Cope A, Meijer A, Zutt I, Reimerink J, Kampmann B, Hoschler K, Zambon M & Tregoning JS (2017) Comparison of mucosal lining fluid sampling methods and influenza-specific IgA detection assays for use in human studies of influenza immunity. Journal of Immunological Methods.
- Garcia-Knight MA, Slyker J, Payne BL, Pond SLK, de Silva TI, Chohan B, Khasimwa B, Mbori-Ngacha D, John-Stewart G, Rowland-Jones SL & Esbjörnsson J (2016) Viral Evolution and Cytotoxic T Cell Restricted Selection in Acute Infant HIV-1 Infection. Scientific Reports, 6. View this article in WRRO
- Fryer HR, Van Tienen C, Van Der Loeff MS, Aaby P, Da Silva ZJ, Whittle H, Rowland-Jones SL & de Silva TI (2015) Predicting the extinction of HIV-2 in rural Guinea-Bissau. AIDS, 29(18), 2479-2486. View this article in WRRO
- Jayasooriya S, de Silva T, Njie-Jobe J, Sanyang C, Leese A, Bell A, McAulay K, Yanchun P, Long HM, Dong T , Whittle HC et al (2015) Early Virological and Immunological Events in Asymptomatic Epstein-Barr Virus Infection in African Children. PLoS Pathogens, 11(3). View this article in WRRO
- de Silva TI, Green ST, Cole J, Stone BJ, Dockrell DH & Vedio AB (2014) Successful use of Fendrix in HIV-infected non-responders to standard hepatitis B vaccines. Journal of Infection, 68(4), 397-399.
- de Silva TI, Peng Y, Leligdowicz A, Zaidi I, Li L, Griffin H, Blais M-E, Vincent T, Saraiva M, Yindom L-M , van Tienen C et al (2013) Correlates of T-cell-mediated viral control and phenotype of CD8+ T cells in HIV-2, a naturally contained human retroviral infection. Blood, 121(21), 4330-4339.
- Daigneault M, De Silva TI, Bewley MA, Preston JA, Marriott HM, Mitchell AM, Mitchell TJ, Read RC, Whyte MKB & Dockrell DH (2012) Monocytes Regulate the Mechanism of T-cell Death by Inducing Fas-Mediated Apoptosis during Bacterial Infection. PLoS Pathogens, 8(7). View this article in WRRO