Dr Claire Turner
0114 222 2819
Microbiology, Streptococcus pyogenes, Group A streptococcus, bacterial evolution, scarlet fever, necrotising fasciitis, tonsilitis, whole genome sequencing, recombination.
My research focus is on trying to understand the mechanisms behind upsurges in disease caused by the human pathogen Streptococcus pyogenes (also known as group A Streptococcus). Upsurges occur frequently and can be associated with specific genotypes. I use a combination of whole genome sequencing, epidemiology, clinical analysis and phenotypic work to try and identify what makes strains successful at causing disease and become suddenly more abundant.
Figure 1. Whole genome sequencing of 131 isolates of emm89 S. pyogenes from the UK identified an emergent clade variant that differed from the rest of the population of emm89 S. pyogenes by a high number of SNPs. This high level of variation is unexpected within a genotype but, importantly, the majority of the SNPs actually clustered in to six regions within the genome, which is indicative of recombination.
Figure 2. (A) The largest region of recombination with emm89 encompassed the toxin genes nga and slo, which encode for NADase and Streptolysin O, and when combined can be lethal to host cells. These toxins are also thought to promote survival of S. pyogenes inside host cells. Compared to the previous variant, this region in the emergent variant contained a high number of SNPs (indicated by vertical lines; black = synonymous, red = non-synonymous) indicating that this region had been obtained from an external donor. (B) The expression of nga was high in the emergent variant (red) compared to nearly undetectable levels in the previous emm89 variant (blue).
If you interested in joining the group then please contact me directly by email at firstname.lastname@example.org.
I have a FULLY FUNDED PhD studentship, available to home, EU or international students. If you wish to self fund a PhD then please contact me directly to discuss potential projects.
- Turner CE, Bedford L, Brown NM, Judge K, Török ME, Parkhill J & Peacock SJ (2017) Community outbreaks of group A Streptococcus revealed by genome sequencing. Scientific Reports, 7(1). View this article in WRRO
- Afshar B, Turner CE, Lamagni T, Smith K, Al-Shahib A, Underwood A, Holden M, Efstratiou A & Sriskandan S (2017) Enhanced nasopharyngeal infection and shedding associated with an epidemic lineage of emm3 group A Streptococcus. Virulence. View this article in WRRO
- Al-Shahib A, Underwood A, Afshar B, Turner CE, Lamagni T, Sriskandan S & Efstratiou A (2016) Emergence of a novel lineage containing a prophage in emm/M3 group A Streptococcus associated with upsurge in invasive disease in the UK. Microbial Genomics, 2(6). View this article in WRRO
- Turner CE, Pyzio M, Song B, Lamagni T, Meltzer M, Chow JY, Efstratiou A, Curtis S & Sriskandan S (2016) Scarlet fever upsurge in england and molecular-genetic analysis in North-West London, 2014. Emerging Infectious Diseases, 22(6), 1075-1078.
- Gierula M, Turner CE, Mosavie M, Siggins MK, Sriskandan S, Edwards RJ, Pichon B, Lynskey NN, Kearns AM & Smith DS (2016) Identification of commonly expressed exoproteins and proteolytic cleavage events by proteomic mining of clinically relevant UK isolates of Staphylococcus aureus. Microbial Genomics, 2(2). View this article in WRRO
- Turner CE, Lamagni T, Holden MTG, David S, Jones MD, Game L, Efstratiou A & Sriskandan S (2015) Turner et al. Reply to “Emergence of the Same Successful Clade among Distinct Populations of emm 89 Streptococcus pyogenes in Multiple Geographic Regions”. mBio, 6(6), e01883-15-e01883-15. View this article in WRRO
- Turner CE, Abbott J, Lamagni T, Holden MTG, David S, Jones MD, Game L, Efstratiou A & Sriskandan S (2015) Emergence of a New Highly Successful Acapsular Group A Streptococcus Clade of Genotype emm 89 in the United Kingdom. mBio, 6(4), e00622-15-e00622-15. View this article in WRRO
- Turner CE & Sriskandan S (2015) Panton–Valentine leucocidin expression by Staphylococcus aureus exposed to common antibiotics. Journal of Infection, 71(3), 338-346.
- Lynskey NN, Turner CE, Heng LS & Sriskandan S (2015) A Truncation in the Regulator RocA Underlies Heightened Capsule Expression in Serotype M3 Group A Streptococci. Infection and Immunity, 83(4), 1732-1733. View this article in WRRO
- Lynskey NN, Goulding D, Gierula M, Turner CE, Dougan G, Edwards RJ & Sriskandan S (2013) RocA Truncation Underpins Hyper-Encapsulation, Carriage Longevity and Transmissibility of Serotype M18 Group A Streptococci. PLoS Pathogens, 9(12), e1003842-e1003842.
- Alam FM, Bateman C, Turner CE, Wiles S & Sriskandan S (2013) Non-Invasive Monitoring of Streptococcus pyogenes Vaccine Efficacy Using Biophotonic Imaging. PLoS ONE, 8(11), e82123-e82123. View this article in WRRO
- Turner CE, Dryden M, Holden MTG, Davies FJ, Lawrenson RA, Farzaneh L, Bentley SD, Efstratiou A & Sriskandan S (2013) Molecular Analysis of an Outbreak of Lethal Postpartum Sepsis Caused by Streptococcus pyogenes. Journal of Clinical Microbiology, 51(7), 2089-2095. View this article in WRRO
- Alam FM, Turner CE, Smith K, Wiles S & Sriskandan S (2013) Inactivation of the CovR/S Virulence Regulator Impairs Infection in an Improved Murine Model of Streptococcus pyogenes Naso-Pharyngeal Infection. PLoS ONE, 8(4), e61655-e61655.
- Turner CE, Sommerlad M, McGregor K, Davies FJ, Pichon B, Chong DLW, Farzaneh L, Holden MTG, Spratt BG, Efstratiou A & Sriskandan S (2012) Superantigenic Activity of emm3 Streptococcus pyogenes Is Abrogated by a Conserved, Naturally Occurring smeZ Mutation. PLoS ONE, 7(10), e46376-e46376. View this article in WRRO
- Kurupati P, Turner CE, Tziona I, Lawrenson RA, Alam FM, Nohadani M, Stamp GW, Zinkernagel AS, Nizet V, Edwards RJ & Sriskandan S (2010) Chemokine-cleaving Streptococcus pyogenes protease SpyCEP is necessary and sufficient for bacterial dissemination within soft tissues and the respiratory tract. Molecular Microbiology, 76(6), 1387-1397.
- Turner CE, Kurupati P, Jones MD, Edwards RJ & Sriskandan S (2009) Emerging Role of the Interleukin‐8 Cleaving Enzyme SpyCEP in Clinical Streptococcus pyogenes Infection. The Journal of Infectious Diseases, 200(4), 555-563.
- Turner CE, Kurupati P, Wiles S, Edwards RJ & Sriskandan S (2009) Impact of immunization against SpyCEP during invasive disease with two streptococcal species: Streptococcus pyogenes and Streptococcus equi. Vaccine, 27(36), 4923-4929.
- Zinkernagel AS, Timmer AM, Pence MA, Locke JB, Buchanan JT, Turner CE, Mishalian I, Sriskandan S, Hanski E & Nizet V (2008) The IL-8 Protease SpyCEP/ScpC of Group A Streptococcus Promotes Resistance to Neutrophil Killing. Cell Host & Microbe, 4(2), 170-178.
- Turner C & Sriskandan S (2007) Streptococcus pyogenes under pressure. Nature Medicine, 13(8), 909-910.
- Edwards RJ, Taylor GW, Ferguson M, Murray S, Rendell N, Wrigley A, Bai Z, Boyle J, Finney SJ, Jones A, Russell HH, Turner C, Cohen J, Faulkner L & Sriskandan S (2005) Specific C‐Terminal Cleavage and Inactivation of Interleukin‐8 by Invasive Disease Isolates of Streptococcus pyogenes. The Journal of Infectious Diseases, 192(5), 783-790.
- Efstratiou A, Lamagni T & Turner CE (2016) Streptococci and Enterococci In Cohen J, Powderly W & Opal S (Ed.), Infectious Diseases 4th edition Elsevier