Dr Thushan de Silva

MBChB BSc (Hons) DTM&H MRCP FRCPath PhD

Department of Infection, Immunity and Cardiovascular Disease

Senior Clinical Lecturer in Infectious Diseases and Honorary Consultant Physician in Infectious Diseases

t.desilva@sheffield.ac.uk
+44 114 215 9522

+44 114 271 1863 (Fax)

The Medical School

Full contact details

Dr Thushan de Silva
Department of Infection, Immunity and Cardiovascular Disease
The Medical School
Beech Hill Road
Sheffield
S10 2RX

Profile

I completed my undergraduate medical training at the University of Bristol in 2000. Following postgraduate clinical training in General Internal Medicine, Infectious Diseases and HIV in Oxford and London, I moved to Sheffield for higher specialist training in Infectious Diseases & Microbiology in 2004. I was awarded a British Infection Society Research Fellowship (2007-8), followed by an MRC Clinical Research Training Fellowship and PhD (2008 – 11) under the supervision of Professor Sarah Rowland-Jones (Oxford/Sheffield) and Professor Robin Weiss (UCL) to study the pathogenesis of HIV-2 infection and the molecular epidemiology of HIV-1 and HIV-2 in West Africa, based primarily at the MRC Unit The Gambia.

I joined the University of Sheffield in 2013 as an NIHR Academic Clinical Lecturer in Infectious Diseases & Microbiology and was awarded an Academy of Medical Sciences starter grant to undertake pilot studies of live attenuated influenza vaccination (LAIV) in adult volunteers. Data generated during these lead to the successful award of a Wellcome Trust Intermediate Clinical Fellowship in 2016 under the sponsorship of Professor Beate Kampmann (MRC Unit The Gambia/LSHTM), based between Imperial College London and the MRC Unit The Gambia.

I returned to the University of Sheffield as a Senior Clinical Lecturer in 2020. I am part of the Academic Clinical Infection Group in the Department of Infection, Immunity and Cardiovascular Disease (IICD), along with Prof. Sarah Rowland-Jones, Dr. Tom Darton and Dr. Paul Collini. I have been an Honorary Consultant Physician in Infectious Diseases at the South Yorkshire Regional Department of Infection and Tropical Medicine in Sheffield since 2016. I continue to be a Visiting Scientist and Principal Investigator in the Vaccines and Immunity Theme at the MRC Unit The Gambia at LSHTM and am also an Honorary Associate Professor at the London School of Hygiene and Tropical Medicine.

Research interests

My work focuses broadly on the following areas and is increasingly integrating next-generation sequencing data with immunology, clinical and epidemiological data derived from clinical trials and observational studies in human cohorts:

Anti-viral immune responses in the context of vaccination and natural infection, Building on skills gained during my PhD, which characterised HIV-specific humoral and T-cell correlates of protection from disease progression in HIV-2 infection in West Africa, a major focus now is on the innate and adaptive immune response to influenza and influenza vaccines. I am now also involved in studies characterising the magnitude and durability of SARS-CoV-2 specific T-cell responses across the COVID-19 disease spectrum.

SARS-CoV-2 molecular epidemiology. As part of the COG-UK consortium, I am leading SARS-CoV-2 sequencing in Sheffield, including a specific focus on the use of rapid sequencing in tracking hospital transmission and utility to infection, prevention and control. I am also part of efforts to enhance the availability of SARS-CoV-2 sequence data and analysis in West Africa.

The molecular epidemiology of potentially vaccine-preventable infections that have a high burden yet are relatively neglected in low and middle-income countries. E.g. influenza, SARS-CoV-2 and other respiratory viruses, Streptococcus pyogenes, Staphylococcus aureus.

Key Current Projects

Immunogenicity of intranasal live attenuated influenza vaccination (LAIV) and bidirectional interactions with the host microbiome in Gambian children (Wellcome Trust Intermediate Clinical Fellowship, 2016 – 2020). Key collaborators: Dr. Helder Nakaya, University of Sao Paulo and Prof. Debby Bogaert, University of Edinburgh.
University of Sheffield lead for the COVID-19 Genomics Consortium UK (COG-UK), using Oxford Nanopore Technology to sequence SARS-CoV-2 isolates in South Yorkshire. This involves a key collaboration with Dr. Matthew Parker at the Sheffield Bioinformatics Core.
Study of SARS-CoV-2 T-cell responses as part of the ISARIC4C consortium and UK Coronavirus Immunology Consortium (UK-CIC).
Surveillance for SARS-CoV-2 and other respiratory viruses by establishment and expansion of a sentinel surveillance platform for influenza-like illness in The Gambia, with Prof. Beate Kampmann.
Immunological imprinting of Live Attenuated Influenza Vaccine in children naïve vs exposed to influenza viruses; with Prof. Florian Krammer, Icahn School of Medicine at Mount Sinai, New York (Bill and Melinda Gates Foundation, 2019 – 2021).
Several clinical and molecular epidemiology studies forming part of the MRC Unit The Gambia Strep A research portfolio, as part of a Strategic Partnership Award between the University of Sheffield and the MRC Gambia (UoS GCRF), with Dr. Claire Turner, MBB, and key collaborators Dr. Michael Marks, LSHTM; Dr. Annette Erhart, MRCG and Dr. Abdul Karim Sesay, MRCG.
Use of differentiated nasal epithelial cell air liquid interface models to explore early host-influenza interactions; with Prof. Colin Bingle, IICD (Wellcome Trust Intermediate Clinical Fellowship, 2020 – 2021).

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Weissman D, Alameh M-G, de Silva T, Collini P, Hornsby H, Brown R, LaBranche CC, Edwards RJ, Sutherland L, Santra S , Mansouri K et al (2020) D614G Spike mutation increases SARS CoV-2 susceptibility to neutralization. Cell Host & Microbe.
Korber B, Fischer WM, Gnanakaran S, Yoon H, Theiler J, Abfalterer W, Hengartner N, Giorgi EE, Bhattacharya T, Foley B , Hastie KM et al (2020) Tracking changes in SARS-CoV-2 Spike: evidence that D614G increases infectivity of the COVID-19 virus. Cell. View this article in WRRO
Minter A, Hoschler K, Jagne YJ, Sallah H, Armitage E, Lindsey B, Hay JA, Riley S, de Silva TI & Kucharski A (2020) Estimation of seasonal influenza attack rates and antibody dynamics in children using cross-sectional serological data. The Journal of Infectious Diseases. View this article in WRRO
Jarju S, Greenhalgh K, Wathuo M, Banda M, Camara B, Mendy S, Sowe G, Dahaba PO, Jammeh L, Bajinka Y , Sanneh P et al (2020) Viral Etiology, Clinical Features and Antibiotic Use in Children <5 Years of Age in the Gambia Presenting With Influenza-like Illness. Pediatric Infectious Disease Journal, Publish Ahead of Print.
Keeley AJ, Evans C, Colton H, Ankcorn M, Cope A, State A, Bennett T, Giri P, de Silva TI & Raza M (2020) Roll-out of SARS-CoV-2 testing for healthcare workers at a large NHS foundation trust in the United Kingdom, March 2020. Eurosurveillance, 25(14). View this article in WRRO
Mackenzie GA, Vilane A, Salaudeen R, Hogerwerf L, van den Brink S, Wijsman LA, Overduin P, Janssens TKS, de Silva TI, van der Sande MAB , Kampmann B et al (2019) Respiratory syncytial, parainfluenza and influenza virus infection in young children with acute lower respiratory infection in rural Gambia. Scientific Reports, 9(1).
Armitage EP, Senghore E, Darboe S, Barry M, Camara J, Bah S, Marks M, Cerami C, Roca A, Antonio M , Turner CE et al (2019) High burden and seasonal variation of paediatric scabies and pyoderma prevalence in the Gambia : a cross-sectional study. PLOS Neglected Tropical Diseases, 13(10). View this article in WRRO
de Lima DS, Cardozo LE, Maracaja-Coutinho V, Suhrbier A, Mane K, Jeffries D, Silveira ELV, Amaral PP, Rappuoli R, de Silva TI & Nakaya HI (2019) Long noncoding RNAs are involved in multiple immunological pathways in response to vaccination. Proceedings of the National Academy of Sciences, 116(34), 17121-17126. View this article in WRRO
Lindsey BB, Jagne YJ, Armitage EP, Singanayagam A, Sallah HJ, Drammeh S, Senghore E, Mohammed NI, Jeffries D, Höschler K , Tregoning JS et al (2019) Effect of a Russian-backbone live-attenuated influenza vaccine with an updated pandemic H1N1 strain on shedding and immunogenicity among children in The Gambia : an open-label, observational, phase 4 study. The Lancet Respiratory Medicine. View this article in WRRO
Miller PDE, de Silva TI, Leonard H, Anthias C, Hoschler K, Goddard K, Peggs K, Madrigal A & Snowden JA (2019) A comparison of viral microneutralization and haemagglutination inhibition assays as measures of seasonal inactivated influenza vaccine immunogenicity in the first year after reduced intensity conditioning, lymphocyte depleted allogeneic haematopoietic stem cell transplant. Vaccine, 37(3), 452-457. View this article in WRRO
Lindsey BB, Armitage EP, Kampmann B & de Silva TI (2018) The efficacy, effectiveness, and immunogenicity of influenza vaccines in Africa: a systematic review. The Lancet Infectious Diseases. View this article in WRRO
de Silva T, Leligdowicz A, Carlson J, Garcia-Knight M, Onyango C, Miller N, Yindom LM, Hue S, Jaye A, Dong T , Cotten M et al (2018) HLA-associated polymorphisms in the HIV-2 capsid highlight key differences between HIV-1 and HIV-2 immune adaptation. AIDS, 6, 704-709. View this article in WRRO
de Silva TI, Gould V, Mohammed NI, Cope A, Meijer A, Zutt I, Reimerink J, Kampmann B, Hoschler K, Zambon M & Tregoning JS (2017) Comparison of mucosal lining fluid sampling methods and influenza-specific IgA detection assays for use in human studies of influenza immunity. Journal of Immunological Methods.
Fryer HR, Van Tienen C, Van Der Loeff MS, Aaby P, Da Silva ZJ, Whittle H, Rowland-Jones SL & de Silva TI (2015) Predicting the extinction of HIV-2 in rural Guinea-Bissau. AIDS, 29(18), 2479-2486. View this article in WRRO
Jayasooriya S, de Silva TI, Njie-jobe J, Sanyang C, Leese AM, Bell AI, McAulay KA, Yanchun P, Long HM, Dong T , Whittle HC et al (2015) Early Virological and Immunological Events in Asymptomatic Epstein-Barr Virus Infection in African Children. PLOS Pathogens, 11(3). View this article in WRRO
de Silva TI, Green ST, Cole J, Stone BJ, Dockrell DH & Vedio AB (2014) Successful use of Fendrix in HIV-infected non-responders to standard hepatitis B vaccines. Journal of Infection, 68(4), 397-399.
de Silva TI, Peng Y, Leligdowicz A, Zaidi I, Li L, Griffin H, Blais M-E, Vincent T, Saraiva M, Yindom L-M , van Tienen C et al (2013) Correlates of T-cell–mediated viral control and phenotype of CD8+ T cells in HIV-2, a naturally contained human retroviral infection. Blood, 121(21), 4330-4339.
de Silva TI, Aasa-Chapman M, Cotten M, Hue S, Robinson J, Bibollet-Ruche F, Sarge-Njie R, Berry N, Jaye A, Aaby P , Whittle H et al (2012) Potent Autologous and Heterologous Neutralizing Antibody Responses Occur in HIV-2 Infection across a Broad Range of Infection Outcomes. Journal of Virology, 86(2), 930-946.
de Silva TI, Turner R, Hué S, Trikha R, van Tienen C, Onyango C, Jaye A, Foley B, Whittle H, Rowland-Jones SL & Cotten M (2010) HIV-1 subtype distribution in the Gambia and the significant presence of CRF49_cpx, a novel circulating recombinant form. Retrovirology, 7(1). View this article in WRRO
Lindsey B, Jagne YJ, Armitage E, Singanayagam A, Sallah H, Drammeh S, Senghore E, Mohammed N, Jeffries D, Höschler K , Tregoning J et al () The impact of an updated pandemic H1N1 strain on shedding and immunogenicity to Russian-backbone live attenuated influenza vaccine among children in The Gambia: an open-label, observational, phase 4 study.. The Lancet Respiratory Medicine.

All publications

Journal articles

Abaleke E, Abbas M, Abbasi S, Abbott A, Abdelaziz A, Abdelbadiee S, Abdelfattah M, Abdul B, Abdul Rasheed A, Abdul-Kadir R , Abdulmumeen A et al (2021) Azithromycin in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial. The Lancet.
Williams EJ, Mair L, de Silva TI, Green DJ, House P, Cawthron K, Gillies C, Wigfull J, Parsons H & Partridge DG (2021) Evaluation of procalcitonin as a contribution to antimicrobial stewardship in SARS-CoV-2 infection: a retrospective cohort study. Journal of Hospital Infection.
Volz E, Hill V, McCrone JT, Price A, Jorgensen D, O’Toole Á, Southgate J, Johnson R, Jackson B, Nascimento FF , Rey SM et al (2021) Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity. Cell, 184(1), 64-75.e11.
Jagne I, Keeley A, Bojang A, Camara B, Jallow E, Senghore E, Oluwalana C, Bah S, Turner C, Sesay AK , D’Alessandro U et al (2020) Impact of intra-partum Azithromycin on carriage of group A streptococcus in The Gambia: a posthoc analysis of a double-blind randomized placebo-controlled trial.
Weissman D, Alameh M-G, de Silva T, Collini P, Hornsby H, Brown R, LaBranche CC, Edwards RJ, Sutherland L, Santra S , Mansouri K et al (2020) D614G Spike mutation increases SARS CoV-2 susceptibility to neutralization. Cell Host & Microbe.
Remmington A, Haywood S, Edgar J, Green LR, de Silva T & Turner CE (2020) Cryptic prophages within a Streptococcus pyogenes genotype emm4 lineage. Microbial Genomics.
Boshier FAT, Pang J, Penner J, Hughes J, Parker M, Shepherd J, Alders N, Bamford A, Grandjean L, Grunewald S , Hatcher J et al (2020) Remdesivir induced viral RNA and subgenomic RNA suppression, and evolution of viral variants in SARS-CoV-2 infected patients.
Drake TM, Docherty AB, Harrison EM, Quint JK, Adamali H, Agnew S, Babu S, Barber CM, Barratt S, Bendstrup E , Bianchi S et al (2020) Outcome of Hospitalization for COVID-19 in Patients with Interstitial Lung Disease. An International Multicenter Study. American Journal of Respiratory and Critical Care Medicine, 202(12), 1656-1665.
Ogbe A, Kronsteiner B, Skelly D, Pace M, Brown A, Adland E, Adair K, Akhter HD, Ali M, Ali S-E , Angyal A et al (2020) T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses.
Peto L, Rodger G, Carter D, Osman K, Yavuz M, Johnson K, Raza M, Parker M, Wyles M, Andersson M , Justice A et al (2020) Diagnosis of SARS-CoV-2 infection with LamPORE, a high-throughput platform combining loop-mediated isothermal amplification and nanopore sequencing.
ten-Caten F, Gonzalez-Dias P, Castro Í, Ogava R, Giddaluru J, Silva JC, Martins F, Gonçalves A, Costa-Martins A, Araujo J , Viegas AC et al (2020) In-depth Analysis of Laboratory Parameters Reveals the Interplay Between Sex, Age and Systemic Inflammation in Individuals with COVID-19.
Korber B, Fischer WM, Gnanakaran S, Yoon H, Theiler J, Abfalterer W, Hengartner N, Giorgi EE, Bhattacharya T, Foley B , Hastie KM et al (2020) Tracking changes in SARS-CoV-2 Spike: evidence that D614G increases infectivity of the COVID-19 virus. Cell. View this article in WRRO
The COVID-19 Genomics UK (COG-UK) consortium , Wang D, Angyal A, Brown RL, Carrilero L, de Silva TI, Green LR, Groves DC, Keeley AJ, Lindsey BB , Parker MD et al (2020) An integrated national scale SARS-CoV-2 genomic surveillance network. The Lancet Microbe, 1(3), e99-e100. View this article in WRRO
Keeley AJ, Evans CM & de Silva TI (2020) Asymptomatic SARS-CoV-2 infection: the tip or the iceberg?. Thorax, 75(8), 621-622.
Minter A, Hoschler K, Jagne YJ, Sallah H, Armitage E, Lindsey B, Hay JA, Riley S, de Silva TI & Kucharski A (2020) Estimation of seasonal influenza attack rates and antibody dynamics in children using cross-sectional serological data. The Journal of Infectious Diseases. View this article in WRRO
Colton H, Ankcorn M, Yavuz M, Tovey L, Cope A, Raza M, Keeley AJ, State A, Poller B, Parker M , de Silva TI et al (2020) Improved sensitivity using a dual target, E and RdRp assay for the diagnosis of SARS-CoV-2 infection : experience at a large NHS Foundation Trust in the UK. Journal of Infection.
Jarju S, Greenhalgh K, Wathuo M, Banda M, Camara B, Mendy S, Sowe G, Dahaba PO, Jammeh L, Bajinka Y , Sanneh P et al (2020) Viral Etiology, Clinical Features and Antibiotic Use in Children <5 Years of Age in the Gambia Presenting With Influenza-like Illness. Pediatric Infectious Disease Journal, Publish Ahead of Print.
Keeley AJ, Evans C, Colton H, Ankcorn M, Cope A, State A, Bennett T, Giri P, de Silva TI & Raza M (2020) Roll-out of SARS-CoV-2 testing for healthcare workers at a large NHS foundation trust in the United Kingdom, March 2020. Eurosurveillance, 25(14). View this article in WRRO
Mackenzie GA, Vilane A, Salaudeen R, Hogerwerf L, van den Brink S, Wijsman LA, Overduin P, Janssens TKS, de Silva TI, van der Sande MAB , Kampmann B et al (2019) Respiratory syncytial, parainfluenza and influenza virus infection in young children with acute lower respiratory infection in rural Gambia. Scientific Reports, 9(1).
Armitage EP, Senghore E, Darboe S, Barry M, Camara J, Bah S, Marks M, Cerami C, Roca A, Antonio M , Turner CE et al (2019) High burden and seasonal variation of paediatric scabies and pyoderma prevalence in the Gambia : a cross-sectional study. PLOS Neglected Tropical Diseases, 13(10). View this article in WRRO
Darboe S, Okomo U, Muhammad A-K, Ceesay B, Jallow M, Usuf E, Tweed S, Akpalu E, Kwambana-Adams B, Kariuki S , Antonio M et al (2019) Community-acquired invasive bacterial disease in urban Gambia, 2005–2015: A hospital-based surveillance. Clinical Infectious Diseases, 69(Supplement_2), S105-S113. View this article in WRRO
de Lima DS, Cardozo LE, Maracaja-Coutinho V, Suhrbier A, Mane K, Jeffries D, Silveira ELV, Amaral PP, Rappuoli R, de Silva TI & Nakaya HI (2019) Long noncoding RNAs are involved in multiple immunological pathways in response to vaccination. Proceedings of the National Academy of Sciences, 116(34), 17121-17126. View this article in WRRO
Lindsey BB, Jagne YJ, Armitage EP, Singanayagam A, Sallah HJ, Drammeh S, Senghore E, Mohammed NI, Jeffries D, Höschler K , Tregoning JS et al (2019) Effect of a Russian-backbone live-attenuated influenza vaccine with an updated pandemic H1N1 strain on shedding and immunogenicity among children in The Gambia : an open-label, observational, phase 4 study. The Lancet Respiratory Medicine. View this article in WRRO
Darboe S, Dobreniecki S, Jarju S, Jallow M, Mohammed NI, Wathuo M, Ceesay B, Tweed S, Basu Roy R, Okomo U , Kwambana-Adams B et al (2019) Prevalence of Panton-Valentine Leukocidin (PVL) and antimicrobial resistance in community-acquired clinical Staphylococcus aureus in an urban Gambian hospital : a 11-year period retrospective pilot study. Frontiers in Cellular and Infection Microbiology, 9. View this article in WRRO
McTavish S, Snow S, Cook E, Pichon B, Coleman S, Coombs G, Pang S, Arias C, Diaz L, Boldock E , Davies S et al (2019) Genomic and epidemiological evidence of a dominant Panton-Valentine leucocidin-positive Methicillin Resistant Staphylococcus aureus lineage in Sri Lanka and presence among isolates from the United Kingdom and Australia. Frontiers in Cellular and Infection Microbiology, 9. View this article in WRRO
Miller PDE, de Silva TI, Leonard H, Anthias C, Hoschler K, Goddard K, Peggs K, Madrigal A & Snowden JA (2019) A comparison of viral microneutralization and haemagglutination inhibition assays as measures of seasonal inactivated influenza vaccine immunogenicity in the first year after reduced intensity conditioning, lymphocyte depleted allogeneic haematopoietic stem cell transplant. Vaccine, 37(3), 452-457. View this article in WRRO
Lindsey BB, Armitage EP, Kampmann B & de Silva TI (2018) The efficacy, effectiveness, and immunogenicity of influenza vaccines in Africa: a systematic review. The Lancet Infectious Diseases. View this article in WRRO
James KL, de Silva TI, Brown K, Whittle H, Taylor S, McVean G, Esbjörnsson J & Rowland-Jones SL (2018) Low-Bias RNA Sequencing of the HIV-2 Genome from Blood Plasma. Journal of Virology, 93(1). View this article in WRRO
Lindsey B, Jankey J, Armitage E, Jeffries D, Mohammed N, Drammeh S, Senghore E, Sallah H, Tregoning J, Hoschler K , Dong T et al (2018) 1663. Marked Improvement in Pandemic H1N1 Component Shedding and Immunogenicity in 2017–2018 Russian-Backbone Live Attenuated Influenza Vaccine (LAIV) in Gambian Children. Open Forum Infectious Diseases, 5(suppl_1), S50-S50.
Miller PDE, Forster AS, de Silva TI, Leonard H, Anthias C, Mayhew M, Klammer M, Paskar S, Hurst E, Peggs K , Madrigal A et al (2018) Sociodemographic and psychological determinants of influenza vaccine intention among recipients of autologous and allogeneic haematopoietic stem cell transplant: a cross-sectional survey of UK transplant recipients using a modified health belief model. BMJ Open, 8(8), e021222-e021222. View this article in WRRO
Knight GM, Clarkson M & de Silva TI (2018) Potential impact of influenza vaccine roll-out on antibiotic use in Africa.. Journal of Antimicrobial Chemotherapy, 73(8), 2197-2200. View this article in WRRO
de Silva T, Leligdowicz A, Carlson J, Garcia-Knight M, Onyango C, Miller N, Yindom LM, Hue S, Jaye A, Dong T , Cotten M et al (2018) HLA-associated polymorphisms in the HIV-2 capsid highlight key differences between HIV-1 and HIV-2 immune adaptation. AIDS, 6, 704-709. View this article in WRRO
Armitage EP, Camara J, Bah S, Forster AS, Clarke E, Kampmann B & de Silva TI (2018) Acceptability of intranasal live attenuated influenza vaccine, influenza knowledge and vaccine intent in The Gambia. Vaccine, 36(13), 1772-1780. View this article in WRRO
Miller PDE, de Silva TI, Skinner R, Gilleece M, Peniket A, Hamblin A, Greenfield D, Anthias C, Peggs K, Madrigal A & Snowden JA (2017) Erratum: Routine vaccination practice after adult and paediatric allogeneic haematopoietic stem cell transplant: a survey of UK NHS programmes. Bone Marrow Transplantation, 52(7), 1082-1082.
de Silva TI, Gould V, Mohammed NI, Cope A, Meijer A, Zutt I, Reimerink J, Kampmann B, Hoschler K, Zambon M & Tregoning JS (2017) Comparison of mucosal lining fluid sampling methods and influenza-specific IgA detection assays for use in human studies of influenza immunity. Journal of Immunological Methods.
de Silva TI & Kampmann B (2017) Value of immunogenicity studies of influenza vaccine in resource-limited settings. The Lancet Global Health, 5(3), e274-e274. View this article in WRRO
Miller PD, de Silva TI, Skinner R, Gilleece M, Peniket A, Hamblin A, Greenfield D, Anthias C, Peggs K, Madrigal A & Snowden JA (2017) Routine vaccination practice after adult and paediatric allogeneic haematopoietic stem cell transplant: a survey of UK NHS programmes.. Bone Marrow Transplant. View this article in WRRO
Parkash V, Mudhar HS, Wagner BE, Raoult D, Batty R, Lepidi H, Burke J, Collini P & de Silva T (2017) Bilateral Ocular Myositis Associated with Whipple's Disease. Ocular Oncology and Pathology, 3(1), 17-21. View this article in WRRO
Garcia-Knight MA, Slyker J, Payne BL, Pond SLK, de Silva TI, Chohan B, Khasimwa B, Mbori-Ngacha D, John-Stewart G, Rowland-Jones SL & Esbjörnsson J (2016) Viral Evolution and Cytotoxic T Cell Restricted Selection in Acute Infant HIV-1 Infection. Scientific Reports, 6. View this article in WRRO
Fryer HR, Van Tienen C, Van Der Loeff MS, Aaby P, Da Silva ZJ, Whittle H, Rowland-Jones SL & de Silva TI (2015) Predicting the extinction of HIV-2 in rural Guinea-Bissau. AIDS, 29(18), 2479-2486. View this article in WRRO
Jayasooriya S, de Silva TI, Njie-jobe J, Sanyang C, Leese AM, Bell AI, McAulay KA, Yanchun P, Long HM, Dong T , Whittle HC et al (2015) Early Virological and Immunological Events in Asymptomatic Epstein-Barr Virus Infection in African Children. PLOS Pathogens, 11(3). View this article in WRRO
de Silva TI, Debroy Kidambi A, Green ST, Mahadeva U, Mcgregor AC, Levy M & Hardcastle N (2015) Cutaneous leishmaniasis acquired during a brief visit to Cyprus. Journal of Infection, 70(3), 314-316.
Yindom L-M, de Silva TI & Rowland-Jones SL (2015) Identification of a novel three-domain KIR allele:KIR3DL1*087using high-resolution molecular techniques. Tissue Antigens, 85(2), 153-154.
Yindom L-M, de Silva TI & Rowland-Jones SL (2015) KIR3DL1*0250102: a novel three-domain KIR subtype identified in West Africa. Tissue Antigens, 85(2), 151-152.
Yindom L-M, de Silva TI & Rowland-Jones SL (2015) KIR3DL1*0250103: a novel three-domain KIR allele isolated in West African samples. Tissue Antigens, 86(2), 150-151.
Yindom L-M, James K, de Silva TI & Rowland-Jones SL (2015) KIR3DL1*0040102- a novel three-domain KIR subtype isolated from donors of African descent. Tissue Antigens, 86(3), 220-221.
Yindom L-M, de Silva TI & Rowland-Jones SL (2015) Full-length sequence ofKIR3DL1*0310102detected in DNA samples from West Africa. Tissue Antigens, 86(4), 312-313.
Yindom L-M, James K, de Silva TI & Rowland-Jones SL (2015) A novelKIR3DL1*0150103subtype identified in West Africa. Tissue Antigens, 86(1), 70-71.
Yindom L-M, de Silva TI & Rowland-Jones SL (2014) Description of a novelKIR3DL1*0150211allele isolated using molecular techniques. Tissue Antigens, 84(6), 596-597.
Yindom L-M, James K, de Silva TI & Rowland-Jones SL (2014) Isolation of full-length genomic sequences of theKIR3DL1*0040103allele from African donors using sequence-based techniques. Tissue Antigens, 84(6), 594-595.
Yindom L-M, Wong G, de Silva TI & Rowland-Jones SL (2014) Complete genomic sequence ofKIR3DL1*0150102. Tissue Antigens, 84(6), 595-596.
Yindom L-M, Wong G, de Silva TI & Rowland-Jones SL (2014) A new variant of killer-cell immunoglobulin-like receptorKIR3DL1*03101isolated using sequence-based techniques. Tissue Antigens, 84(5), 521-522.
Yindom L-M, James K, de Silva TI & Rowland-Jones SL (2014) Genomic full length sequence of a novel killer-cell immunoglobulin-like receptor,KIR3DL1*0010103identified by sequencing. Tissue Antigens, 84(5), 520-521.
Uchtenhagen H, Schiffner T, Bowles E, Heyndrickx L, LaBranche C, Applequist SE, Jansson M, De Silva T, Back JW, Achour A , Scarlatti G et al (2014) Boosting of HIV-1 Neutralizing Antibody Responses by a Distally Related Retroviral Envelope Protein. The Journal of Immunology, 192(12), 5802-5812.
de Silva TI, Green ST, Cole J, Stone BJ, Dockrell DH & Vedio AB (2014) Successful use of Fendrix in HIV-infected non-responders to standard hepatitis B vaccines. Journal of Infection, 68(4), 397-399.
Yindom L-M, James K, de Silva TI & Rowland-Jones SL (2014) Full-lengthKIR3DL1*022detected in an African donor. Tissue Antigens, 84(4), 427-429.
Yindom L-M, de Silva TI & Rowland-Jones SL (2014) A novelKIR3DL1*0200102allele isolated from a West African donor by sequence-based typing. Tissue Antigens, 83(4), 305-306.
Yindom L-M, de Silva TI & Rowland-Jones SL (2014) Full-length sequence ofKIR3DL1*01501allele found in Sub-Saharan Africa by long-range sequencing. Tissue Antigens, 83(2), 126-127.
de Silva T, Peng Y, Leligdowicz A, Zaidi I, Li L, Griffin H, Blais M-E, Vincent T, Saraiva M, Yindom L-M , van Tienen C et al (2013) The role of CD8+ T-cell responses in the pathogenesis of HIV-2, a naturally contained human retroviral infection. Journal of Infection, 67(4), 344-344.
de Silva TI, Peng Y, Leligdowicz A, Zaidi I, Li L, Griffin H, Blais M-E, Vincent T, Saraiva M, Yindom L-M , van Tienen C et al (2013) Correlates of T-cell–mediated viral control and phenotype of CD8+ T cells in HIV-2, a naturally contained human retroviral infection. Blood, 121(21), 4330-4339.
de Silva TI, van Tienen C, Onyango C, Jabang A, Vincent T, Loeff MFSVD, Coutinho RA, Jaye A, Rowland-Jones S, Whittle H , Cotten M et al (2013) Population dynamics of HIV-2 in rural West Africa. AIDS, 27(1), 125-134.
Vannberg FO, McDermid JM, Jaye A, MRC Gambia HIV Clinical Cohort Study & Wellcome Trust Case Control Consortium 3 (2012) Effect of HIV-2 infection on HIV-1 disease progression.. N Engl J Med, 367(20), 1962.
van Tienen C, Schim van der Loeff M & Whittle H (2012) Effect of HIV-2 Infection on HIV-1 Disease Progression. New England Journal of Medicine, 367(20), 1961-1963.
(2012) Effect of HIV-2 Infection on HIV-1 Disease Progression. New England Journal of Medicine, 367(20), 1961-1963.
Daigneault M, De Silva TI, Bewley MA, Preston JA, Marriott HM, Mitchell AM, Mitchell TJ, Read RC, Whyte MKB & Dockrell DH (2012) Monocytes Regulate the Mechanism of T-cell Death by Inducing Fas-Mediated Apoptosis during Bacterial Infection. PLoS Pathogens, 8(7). View this article in WRRO
Zaidi I, Peterson K, Jeffries D, Whittle H, de Silva T, Rowland-Jones S, Jaye A & de Jong BC (2012) Immune Reconstitution Inflammatory Syndrome and the Influence of T Regulatory Cells: A Cohort Study in the Gambia. PLoS ONE, 7(6). View this article in WRRO
van Tienen C, de Silva TI, Alcantara LCJ, Onyango CO, Jarju S, Gonçalves N, Vincent T, Aaby P, Whittle H, Schim van der Loeff M & Cotten M (2012) Molecular Epidemiology of Endemic Human T-Lymphotropic Virus Type 1 in a Rural Community in Guinea-Bissau. PLoS Neglected Tropical Diseases, 6(6). View this article in WRRO
van Tienen C, McConkey SJ, de Silva TI, Cotten M, Kaye S, Sarge-Njie R, da Costa C, Gonçalves N, Parker J, Vincent T , Jaye A et al (2012) Maternal Proviral Load and Vertical Transmission of Human T Cell Lymphotropic Virus Type 1 in Guinea-Bissau. AIDS Research and Human Retroviruses, 28(6), 584-590.
Sabbah S, Jagne YJ, Zuo J, de Silva T, Ahasan MM, Brander C, Rowland-Jones S, Flanagan KL & Hislop AD (2012) T-cell immunity to Kaposi sarcoma–associated herpesvirus: recognition of primary effusion lymphoma by LANA-specific CD4+ T cells. Blood, 119(9), 2083-2092.
de Silva TI, Aasa-Chapman M, Cotten M, Hue S, Robinson J, Bibollet-Ruche F, Sarge-Njie R, Berry N, Jaye A, Aaby P , Whittle H et al (2012) Potent Autologous and Heterologous Neutralizing Antibody Responses Occur in HIV-2 Infection across a Broad Range of Infection Outcomes. Journal of Virology, 86(2), 930-946.
Stewart B, Jobarteh ML, Sarge-Njie R, Alabi A, de Silva T, Peterson K, Peterson I, Whittle H, Rowland-Jones S, Jaye A , Cotten M et al (2011) Emergence of HBV resistance to lamivudine (3TC) in HIV/HBV co-infected patients in The Gambia, West Africa. BMC Research Notes, 4. View this article in WRRO
van Tienen C, van der Loeff MS, Peterson I, Cotten M, Andersson S, Holmgren B, Vincent T, de Silva T, Rowland-Jones S, Aaby P & Whittle H (2011) HTLV-1 and HIV-2 Infection Are Associated with Increased Mortality in a Rural West African Community. PLoS ONE, 6(12). View this article in WRRO
Peterson I, Togun O, de Silva T, Oko F, Rowland-Jones S, Jaye A & Peterson K (2011) Mortality and immunovirological outcomes on antiretroviral therapy in HIV-1 and HIV-2-infected individuals in the Gambia. AIDS, 25(17), 2167-2175.
Peterson K, Jallow S, Rowland-Jones SL & de Silva TI (2011) Antiretroviral Therapy for HIV-2 Infection: Recommendations for Management in Low-Resource Settings. AIDS Research and Treatment, 2011. View this article in WRRO
van Tienen C, de Silva TI, Alcantara LC, van der Loeff MS, Vincent T, Gonçalves N, Coutinho RA, Aaby P, Whittle H & Cotten M (2011) Molecular epidemiology of endemic Human T-Lymphotropic virus 1 (HTLV-1) in a community in rural Guinea-Bissau. Retrovirology, 8(S1).
van Tienen C, McConkey SJ, de Silva TI, Cotten M, Kaye S, Sarge-Njie R, da Costa C, Gonçalves N, Parker J, Vincent T , Jaye A et al (2011) Maternal proviral load and vertical transmission of Human T cell Lymphotropic Virus type 1 in Guinea-Bissau. Retrovirology, 8(S1).
de Silva T & Weiss RA (2010) HIV-2 goes global: An unaddressed issue in Indian anti-retroviral programmes. Indian Journal of Medical Research, 132(12), 660-662.
de Silva TI, Turner R, Hué S, Trikha R, van Tienen C, Onyango C, Jaye A, Foley B, Whittle H, Rowland-Jones SL & Cotten M (2010) HIV-1 subtype distribution in the Gambia and the significant presence of CRF49_cpx, a novel circulating recombinant form. Retrovirology, 7(1). View this article in WRRO
Onyango CO, Leligdowicz A, Yokoyama M, Sato H, Song H, Nakayama EE, Shioda T, de Silva T, Townend J, Jaye A , Whittle H et al (2010) HIV-2 capsids distinguish high and low virus load patients in a West African community cohort. Vaccine, 28, B60-B67.
de Silva TI, van Tienen C, Rowland-Jones SL & Cotten M (2010) Dual infection with HIV-1 and HIV-2: double trouble or destructive interference?. HIV Therapy, 4(3), 305-323.
Jallow S, Vincent T, Leligdowicz A, De Silva T, Van Tienen C, Alabi A, Sarge‐Njie R, Aaby P, Corrah T, Whittle H , Jaye A et al (2009) Presence of a Multidrug‐Resistance Mutation in an HIV‐2 Variant Infecting a Treatment‐Naive Individual in Caio, Guinea Bissau. Clinical Infectious Diseases, 48(12), 1790-1793.
de Silva TI, Cotten M & Rowland-Jones SL (2008) HIV-2: the forgotten AIDS virus. Trends in Microbiology, 16(12), 588-595.
Stone B, Patel TG, De Silva TI, Green ST & Chapman ALN (2008) Successful treatment of actinomyces brain abscesses using once daily parenteral ceftriaxone in an outpatient setting: a case series. Journal of Infection, 57(5), 426-427.
Rowland-Jones S & de Silva T (2008) Resisting Immune Exhaustion in HIV-1 Infection. PLoS Medicine, 5(5), 721-724. View this article in WRRO
de Silva T, Kudesia G, Joall A, Whittaker D, Ryan C & Davies S (2007) The significance of low-positive MOTA scoresin the BD-ProbeTec-Strand Displacement Amplification test for the detection of Neisseria gonorrhoeae. Journal of Infection, 55(3), e67-e68.
de Silva TI, Post FA, Griffin MD & Dockrell DH (2007) HIV-1 Infection and the Kidney: An Evolving Challenge in HIV Medicine. Mayo Clinic Proceedings, 82(9), 1103-1116. View this article in WRRO
de Silva TI, Cope A, Goepel J & Greig JM (2007) The use of adjuvant granulocyte-macrophage colony-stimulating factor in HIV-related disseminated atypical mycobacterial infection. Journal of Infection, 54(4), e207-e210.
de Silva T, Kudesia G, Joall A, Whittaker D, Davies S & Ryan C (2006) Significance of Low Positive Scores Obtained with a Method Other than Acceleration in the BDProbeTec-Strand Displacement Amplification Test for Detection of Neisseria gonorrhoeae. Journal of Clinical Microbiology, 44(12), 4628-4629.
Raychaudhuri M, de Silva T, Connor S & Poulton M (2006) Haemorrhagic acute disseminated encephalomyelitis: an unusual case of blindness in an HIV-infected patient. International Journal of STD & AIDS, 17(7), 495-497.
Chapman AL, Kudesia G, McKendrick MW & de Silva TI (2006) WITHDRAWN: Ongoing queries: Interpretation of serology in asymptomatic or atypical chronic Q fever. Journal of Infection.
Evans CM, Mudhar H, Rennie I, Green ST & de Silva TI (2006) WITHDRAWN: Spontaneous resolution of endophthalmitis due to nocardia species. Journal of Infection.
de Silva T, Chapman A, Kudesia G & McKendrick M (2006) Ongoing queries: Interpretation of serology in asymptomatic or atypical chronic Q fever. Journal of Infection, 52(4), e113-e116.
de Silva T (2005) Ventriculitis and hydrocephalus: an unusual presentation of toxoplasmosis in an adult with human immunodeficiency virus. Journal of Neurology, Neurosurgery & Psychiatry, 76(8), 1074-1074.
de Silva T (2005) HIV infection associated with Strongyloides stercoralis colitis resulting in Streptococcus bovis bacteraemia and meningitis. Sexually Transmitted Infections, 81(3), 276-277.
Peng Y, Mentzer AJ, Liu G, Yao X, Yin Z, Dong D, Dejnirattisai W, Rostron T, Supasa P, Liu C , López-Camacho C et al () Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19. Nature Immunology.
Peng Y, Mentzer AJ, Liu G, Yao X, Yin Z, Dong D, Dejnirattisai W, Rostron T, Supasa P, Liu C , Lopez-Camacho C et al () Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients.
Adams ER, Ainsworth M, Anand R, Andersson MI, Auckland K, Baillie JK, Barnes E, Beer S, Bell JI, Berry T , Bibi S et al () Antibody testing for COVID-19: A report from the National COVID Scientific Advisory Panel. Wellcome Open Research, 5, 139-139.
Lindsey B, Jagne YJ, Armitage E, Singanayagam A, Sallah H, Drammeh S, Senghore E, Mohammed N, Jeffries D, Höschler K , Tregoning J et al () The impact of an updated pandemic H1N1 strain on shedding and immunogenicity to Russian-backbone live attenuated influenza vaccine among children in The Gambia: an open-label, observational, phase 4 study.. The Lancet Respiratory Medicine.
Rowland-Jones S & de Silva T () Correction: Resisting Immune Exhaustion in HIV-1 Infection. PLoS Medicine, 5(6), e134-e134.
de Silva T, Evans C, Mudhar HS, Rennie I & Green ST () Isolated endogenous endophthalmitis secondary to Nocardia spp in an immunocompetent adult. Journal of Clinical Pathology, 59(11), 1226-1226.
Parker MD, Lindsey BB, Leary S, Gaudieri S, Chopra A, Wyles M, Angyal A, Green LR, Parsons P, Tucker RM , Brown R et al () periscope: sub-genomic RNA identification in SARS-CoV-2 ARTIC Network Nanopore Sequencing Data.
Williams EJ, Mair L, de Silva TI, Green DJ, House P, Cawthron K, Gillies C, Wigfull J, Parsons H & Partridge DG () Routine measurement of serum procalcitonin allows antibiotics to be safely withheld in patients admitted to hospital with SARS-CoV-2 infection.. View this article in WRRO
Goldhill DH, Lindsey B, Kugathasan R, Garza ZF, Jagne YJ, Sallah HJ, Goderski G, van Tol S, Höschler K, Meijer A , Barclay WS et al () The Consequences of Egg Adaptation in the H3N2 Component to the Immunogenicity of Live Attenuated Influenza Vaccine.
Lindsey BB, Höschler K & de Silva TI () Complexities in predicting the immunogenicity of live attenuated influenza vaccines. Clinical Infectious Diseases. View this article in WRRO
Stirrup OT, Hughes J, Parker M, Partridge DG, Shepherd JG, Blackstone J, Coll F, Keeley AJ, Lindsey BB, Marek A , Peters C et al () Rapid feedback on hospital onset SARS-CoV-2 infections combining epidemiological and sequencing data.

Chapters

Abrahamian FM, Aldape MJ, Aldasoro E, Allen UD, Al-Sum H, Anadkat MJ, Anders K, Angelakis E, Angus BJ, Antoniadou A , Arena F et al (2017) List of Contributors, Infectious Diseases (pp. xv-xxxvi). Elsevier
Ball TB, Barré-Sinoussi F, Biasin M, Blankson JN, Broliden K, Buckheit RW, Card CM, Clerici M, de Silva T, Fellay J , Fowke KR et al (2012) Contributors, Models of Protection Against HIV/SIV (pp. xi-xii). Elsevier
Moysi E, de Silva T & Rowland-Jones S (2012) Chapter 11 Immune Mechanisms of Viral Control in HIV-2 Infection, Models of Protection Against HIV/SIV (pp. 293-315).
Moysi E, de Silva T & Rowland-Jones S (2012) Immune Mechanisms of Viral Control in HIV-2 Infection, Models of Protection Against HIV/SIV (pp. 293-315). Elsevier

Conference proceedings papers

Miller PDE, de Silva T, Leonard H, Goddard K, Anthias C, Hoschler K, Peggs K, Madrigal A & Snowden JA (2017) Viral microneutralization assay to determine seasonal influenza vaccine immunogenicity in the first year post reduced intensity conditioning allogeneic HSCT. BONE MARROW TRANSPLANTATION, Vol. 52 (pp S269-S270)
Miller PDE, de Silva T, Skinner R, Gilleece M, Peniket A, Hamblin A, Greenfield D, Anthias C, Peggs K, Madrigal A , Snowden JA et al (2016) Routine Vaccination Programme (RVP) Practice after Adult and Paediatric Allogeneic Haematopoietic Stem Cell Transplant (HSCT): A British Society of Blood and Marrow Transplantation (BSBMT) Survey of UK NHS-Based Programmes. BONE MARROW TRANSPLANTATION, Vol. 51 (pp S400-S401)
Meiring J, De Silva T & Snowden J (2015) A study of adherence to a vaccination schedule following adult allogenic haematopoietic stem cell transplants in UK transplant centre. BONE MARROW TRANSPLANTATION, Vol. 50 (pp S203-S204)

Teaching interests: I am involved in delivering the undergraduate (MBChB) Infectious Diseases, Tropical Medicine and Microbiology curriculum at the University of Sheffield. I also lecture on the MSc in Antimicrobial Resistance (Florey Institute). I am also involved in the Sheffield Diploma in Tropical Medicine and Hygiene (DTM&H) course that prepares candidates to sit the Royal College of Physicians DTM&H examination.

Professional activities

Associate Editor, Journal of Infection (2019 - present)
Associate Editor, Transactions of the Royal Society of Tropical Medicine and Hygiene (2019 - present)
The Florey Institute Management Board, University of Sheffield (2019 - present)
University of Sheffield Biosafety Committee (2019 - present)
British Society of Blood and Marrow Transplantation, post-haematopoietic stem cell transplantation vaccination subgroup (2020 – present)