Dr Hannes Maib

School of Biosciences

Research Fellow

h.maib@sheffield.ac.uk

Addison Building

Full contact details

Dr Hannes Maib
School of Biosciences
Addison Building
Sheffield
S10 2TN

Profile: • 2023 – Present - Wellcome Trust Early Career Fellow, University of Sheffield

• 2019-2023 - Postdoctoral Research Assistant, University of Dundee, Cell and Developmental Biology, Supervisor: Dr. David Murray

• 2015-2019 - PhD University of Sheffield, Supervisor: Prof Elizabeth Smythe

• 2013-2015 - MSc, Life and Medical Sciences, University of Bonn, Germany Supervisor: Prof Thorsten Lang

• 2010-2013 - Bsc, Molekulare Biomedizin, University of Bonn

Research interests: Membrane identity changes in control of contact site formation

Membrane identity is key to cellular function, with each organelle displaying distinct identity determinants. Among the main factors imposing this are phosphoinositides, a small family of phospholipids that act as signpost of membrane identity. Lipid kinases and phosphatases are capable of interconverting these lipids by addition or removal of phosphate groups, leading to 8 distinct phosphoinositides. Key interest of my lab is to understand how these conversion cascades are regulated and how these changes to membrane identity influence cellular processes. The recent realisation that almost all organelles are in constant communication with each other, through formation of dynamic membrane contact sites, has opened up new questions regarding the interplay of membrane identity and contact site formation. Importantly, these contact sites are essential for the intracellular transport of lipids and misregulation is associated with disease and hereditary disorders. Therefore, it is essential to gain a better understanding of the molecular mechanisms that regulate this fundamental aspect of cell biology in order to develop novel interventions.

Publications

Maib H, Adarska P, Hunton R, Vines JH, Strutt D, Bottanelli F & Murray DH (2024) Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides. Journal of Cell Biology, 223(6).
Vines JH, Maib H, Buckley CM, Gueho A, Zhu Z, Soldati T, Murray DH & King JS (2023) A PI(3,5)P2 reporter reveals PIKfyve activity and dynamics on macropinosomes and phagosomes. Journal of Cell Biology, 222(9).
Maib H & Murray DH (2022) A mechanism for exocyst-mediated tethering via Arf6 and PIP5K1C-driven phosphoinositide conversion. Current Biology, 32(13), 2821-2833.e6.
Gill MR, Jarman PJ, Hearnden V, Fairbanks SD, Bassetto M, Maib H, Palmer J, Ayscough KR, Thomas JA & Smythe C (2022) A Ruthenium(II) Polypyridyl Complex Disrupts Actin Cytoskeleton Assembly and Blocks Cytokinesis.. Angew Chem Weinheim Bergstr Ger, 134(27), e202117449.
Maib H, Ferreira F, Vassilopoulos S & Smythe E (2018) Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation. The Journal of Cell Biology, 217(12), 4253-4266.
Maib H, Smythe E & Ayscough KR (2017) Forty years on: clathrin-coated pits continue to fascinate. Molecular Biology of the Cell, 28(7), 843-847.
Edwards-Hicks J, Apostolova P, Buescher JM, Maib H, Stanczak MA, Corrado M, Klein Geltink RI, Maccari ME, Villa M, Carrizo GE , Sanin DE et al () Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function. Nature Immunology.

Maib H, Adarska P, Hunton R, Vines J, Strutt D, Bottanelli F & Murray DH (2023) Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides, Cold Spring Harbor Laboratory.
Alshahrani F, Zhu Z, Ferreira F, McComb A, Maib H, Pruzina S, Robinson D, Murray D & Smythe E (2023) Rme-6 integrates EGFR trafficking and signalling to regulate ERK1/2 signalosome dynamics, Cold Spring Harbor Laboratory.

School of Biosciences