Dr Indrajit Lahiri
School of Biosciences
Lecturer in Electron Microscopy
Full contact details
School of Biosciences
D14A
Firth Court
Western Bank
Sheffield
S10 2TN
- Profile
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2022 - present: Lecturer in Electron Microscopy, University of Sheffield
2019-2022: Assistant Professor, IISER Mohali, India
2015-2019: Postdoctoral Researcher, University of California, San Diego, USA
2014-2015: Postdoctoral Researcher, Harvard University, USA
2008-2014: PhD, State University of New York at Albany, USA
- Research interests
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We study the essential processes of genome replication, repair and compaction in eukaryotic parasites belonging to the phylum Apicomplexa.
Apicomplexans cause deadly diseases like malaria (Plasmodium sp.) and toxoplasmosis (Toxoplasma sp.). These parasites affect over 200 million people worldwide each year. Our interest lies in understanding how apicomplexans carry out DNA transactions within the context of their host - specifically how these processes occur in an organelle called the 'apicoplast'. To answer this question we use electron cryomicroscopy, fast enzyme kinetics, next-generation DNA sequencing and associated biochemical and biophysical techniques.
- Publications
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Journal articles
- Structural basis of multitasking by the apicoplast DNA polymerase from Plasmodium falciparum.. Nucleic Acids Res, 53(19).
- Cryo-EM reconstruction of yeast ADP-actin filament at 2.5 Å resolution. A comparison with vertebrate F-actin. Structure, 33(3), 435-442.e3. View this article in WRRO
- Elf1 promotes Rad26’s interaction with lesion-arrested Pol II for transcription-coupled repair. Proceedings of the National Academy of Sciences, 121(3). View this article in WRRO
- Lis1 relieves cytoplasmic dynein-1 autoinhibition by acting as a molecular wedge. Nature Structural & Molecular Biology, 30(9), 1357-1364. View this article in WRRO
- Transient state kinetics of plasmodium falciparum apicoplast DNA polymerase suggests the involvement of accessory factors for efficient and accurate DNA synthesis. Biochemistry, 61(21), 2319-2333.
- Glu289 residue in the pore-forming motif of Vibrio cholerae cytolysin is important for efficient β-barrel pore formation. Journal of Biological Chemistry, 298(10).
- Editorial: Nucleic Acid Polymerases: The Two-Metal-Ion Mechanism and Beyond. Frontiers in Molecular Biosciences, 9.
- Structural Basis for Cytoplasmic Dynein-1 Regulation by Lis1. Elife, 11.
- Structures and function of the amino acid polymerase cyanophycin synthetase. Nature Chemical Biology, 17(10), 1101-1110.
- Pyrophosphate release acts as a kinetic checkpoint during high-fidelity DNA replication by the Staphylococcus aureus replicative polymerase PolC. Nucleic Acids Research, 49(14), 8324-8338.
- Structure of LRRK2 in Parkinson’s disease and model for microtubule interaction. Nature, 588(7837), 344-349.
- 3.1 Å structure of yeast RNA polymerase II elongation complex stalled at a cyclobutane pyrimidine dimer lesion solved using streptavidin affinity grids. Journal of Structural Biology, 207(3), 270-278.
- cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud. Journal of Structural Biology, 203(3), 230-235.
- Structural basis for the initiation of eukaryotic transcription-coupled DNA repair. Nature, 551(7682), 653-657.
- Three residues of the interdomain linker determine the conformation and single-base deletion fidelity of y-family translesion polymerases. Journal of Biological Chemistry, 289(10), 6323-6331.
- Kinetic Characterization of Exonuclease-Deficient Staphylococcus aureus PolC, a C-family Replicative DNA Polymerase. Plos One, 8(5).
- Human polymerase kappa uses a template-slippage deletion mechanism, but can realign the slipped strands to favour base substitution mutations over deletions. Nucleic Acids Research, 41(9), 5024-5035.
- Map/model validation and machine learning in EM: general discussion. Faraday Discussions, 240, 229-242.
Preprints
- Structural basis of multitasking by the apicoplast DNA polymerase from Plasmodium falciparum, Cold Spring Harbor Laboratory.
- CryoEM Reconstruction of Yeast ADP-Actin Filament at 2.5 Å resolution. A comparison with mammalian and avian F-actin, Cold Spring Harbor Laboratory.
- Elf1 promotes Rad26’s interaction with lesion-arrested Pol II for transcription-coupled repair, Cold Spring Harbor Laboratory.
- Lis1 relieves cytoplasmic dynein-1 autoinhibition by acting as a molecular wedge, Springer Science and Business Media LLC.
- Lis1 relieves cytoplasmic dynein-1 auto-inhibition by acting as a molecular wedge, bioRxiv.
- Structural Basis for Cytoplasmic Dynein-1 Regulation by Lis1, bioRxiv.
- Parkinson’s Disease-linked LRRK2 structure and model for microtubule interaction, bioRxiv.
- Structural basis of multitasking by the apicoplast DNA polymerase from Plasmodium falciparum.. Nucleic Acids Res, 53(19).
- Research group
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Ms. Anamika Kumari (BBSRC White Rose DTP PhD student)
Dr. Anjali Yadav (Postdoctoral researcher)
- Teaching activities
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MBB409