Dr Stephen Brown
School of Biosciences
RNAi Screening Facility Manager
+44 114 222 3690
Full contact details
School of Biosciences
Alfred Denny Building
- 2008 - present: University of Sheffield (School of Biosciences), Sheffield RNAi Screening Facility Manager (SRSF)
- 2004 - 2008: University of Manchester (Faculty of Life Sciences), MRC Career Development Fellowship
- 2004: University of Cambridge (Pathology), Post-Doctoral research
- 1997 - 2003: University of Cambridge (Zoology), Post-Doctoral research
- 1997: University of Nottingham, PhD
- Research interests
Functional genomics, siRNA genome screens, drug screening, cell models of Human disease.
Functional Genomics and RNAi Screening
- Many diseases have an unknown or poorly defined molecular diagnosis. There is a need to identify the molecular nature of these orphan diseases and the changes these mutations cause to cells and tissues.
- The Sheffield RNAi Screening Facility uses robotics and automated systems to leverage RNAi/small molecules and genome editing tools to identify novel biological and disease candidates.
- I use Drosophila and Human cell models of disease. In particular, I use normal, cancer and Human embryonic stem cells. Recently we have been growing 3D tumour spheroids for screening.
- I collaborate with Dr. Xiubo Zhao using self-assembling nano-peptides, Dr. Ivana Barbaric and Dr. Paul Gokhale investigating miR regulation of Human embryonic stem cells and Dr. Martin Zeidler identifying new members of the JAK/STAT signalling pathway.
I have managed over 50 RNAi library screens for both UK and international scientists. My laboratory uses both automation (robotics) and molecular cell biology to identify genes involved in disease. I am also involved in teaching Masters and PhD students in the art of “Functional Genomics”.
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- Surfactant like peptides for targeted gene delivery to cancer cells. Biochemical and Biophysical Research Communications.
- Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides. Frontiers in Bioengineering and Biotechnology, 10. View this article in WRRO
- Chemically-modified dsRNA induces RNAi effects in insects in vitro and in vivo: A potential new tool for improving RNA-based plant protection.. Journal of Biological Chemistry, 102311-102311.
- Peptide-functionalised magnetic silk nanoparticles produced by a swirl mixer for enhanced anticancer activity of ASC-J9. Colloids and Surfaces B: Biointerfaces, 216.
- Rationally designed cationic amphiphilic peptides for selective gene delivery to cancer cells. International Journal of Pharmaceutics, 617.
- Rationally designed short cationic α-helical peptides with selective anticancer activity. Journal of Colloid and Interface Science, 607(Part 1), 488-501. View this article in WRRO
- Designed antitumor peptide for targeted siRNA delivery into cancer spheroids. ACS Applied Materials & Interfaces, 13(42), 49713-49728. View this article in WRRO
- Correlation between the secondary structure and surface activity of β-sheet forming cationic amphiphilic peptides and their anticancer activity. Colloids and Surfaces B: Biointerfaces, 209(Part 2). View this article in WRRO
- Stiffness-tuneable nanocarriers for controlled delivery of ASC-J9 into colorectal cancer cells. Journal of Colloid and Interface Science, 594, 513-521. View this article in WRRO
- Identification and validation of ERK5 as a DNA damage modulating drug target in glioblastoma. Cancers, 13(5). View this article in WRRO
- Direct on-chip differentiation of intestinal tubules from induced pluripotent stem cells. International Journal of Molecular Sciences, 21(14). View this article in WRRO
- Comprehensive functional profiling of long non-coding RNAs through a novel pan-cancer integration approach and modular analysis of their protein-coding gene association networks. BMC Genomics, 20(1), ---. View this article in WRRO
- NOVEL DRUG TARGETS TO AUGMENT TEMOZOLOMIDE SENSITIVITY IN HIGH-GRADE BRAIN TUMOURS. Neuro-Oncology, 20(suppl_5), v346-v346.
- A genome-wide RNAi screen identifies MASK as a positive regulator of cytokine receptor stability.. Journal of Cell Science, 131(13), 0-0. View this article in WRRO
- Compound heterozygous variants in NBAS as a cause of atypical osteogenesis imperfecta.. BONE, 94, 65-74. View this article in WRRO
- Mechanisms of JAK/STAT pathway negative regulation by the short coreceptor Eye Transformer/Latran. Molecular Biology of the Cell, 27(3), 421-598. View this article in WRRO
- Applied RNAi. From Fundamental Research to Therapeutic Applications. Edited by Patrick Arbuthnot and Marc S. Weinberg. ChemMedChem, 10(11), 1934-1935.
- Methotrexate Is a JAK/STAT Pathway Inhibitor. PLoS ONE, 10(7), ---. View this article in WRRO
- Effect of methotrexate on JAK/STAT pathway activation in myeloproliferative neoplasms. The Lancet, 385, S98-S98.
- Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library. Development, 141(20), 3994-4005.
- Genome-wide RNAi screen identifies the Parkinson disease GWAS risk locus SREBF1 as a regulator of mitophagy. Proceedings of the National Academy of Sciences, 111(23), 8494-8499.
- The Sheffield RNAi Screening Facility (SRSF): Portfolio Growth and Technology Development. Combinatorial Chemistry & High Throughput Screening, 17(4), 319-321.
- Putting the 'fun' into functional genomics: A review of RNAi genomewide cellular screens. European Pharmaceutical Review, 17(6).
- Advances in genome-wide RNAi cellular screens: a case study using the Drosophila JAK/STAT pathway. BMC Genomics, 13(1). View this article in WRRO
- Institutional Profile: The Sheffield RNAi screening facility: a service for high-throughput, genome-wide Drosophila RNAi screens. Future Medicinal Chemistry, 2(12), 1805-1812.
- Unphosphorylated STATs go nuclear. Current Opinion in Genetics & Development, 18(5), 455-460.
- Induction of HIF-2α is dependent on mitochondrial O2 consumption in an O2-sensitive adrenomedullary chromaffin cell line. American Journal of Physiology-Cell Physiology, 294(6), C1305-C1312.
- The red flour beetle's large nose: An expanded odorant receptor gene family in Tribolium castaneum. Insect Biochemistry and Molecular Biology, 38(4), 387-397.
- Functional mitochondria are required for O2but not CO2sensing in immortalized adrenomedullary chromaffin cells. American Journal of Physiology-Cell Physiology, 294(4), C945-C956.
- Does chronic stimulation of both nicotinic and opioid receptors contribute to the loss of O2 and CO2 sensitivity in perinatal adrenomedullary chromaffin cells?. The FASEB Journal, 22(S1).
- Role of ATP‐sensitive K + channels in glucose sensing by immortalized adrenomedullary chromaffin cells. The FASEB Journal, 22(S1).
- Stem cell regulation by JAK/STAT signaling in Drosophila. Seminars in Cell & Developmental Biology, 19(4), 407-413.
- Editorial. Seminars in Cell & Developmental Biology, 19(4), 309-310.
- JAK/STAT signalling inDrosophila controls cell motility during germ cell migration. Developmental Dynamics, 235(4), 958-966.
- Characterisation of Upd2, a Drosophila JAK/STAT pathway ligand. Developmental Biology, 288(2), 420-433.
- crossveinless-cis a RhoGAP required for actin reorganisation during morphogenesis. Development, 132(10), 2389-2400.
- Amyloid ? peptides mediate physiological remodelling of the acute O sensitivity of adrenomedullary chromaffin cells following chronic hypoxia. Cardiovascular Research, 64(3), 536-543.
- Acute and chronic hypoxic regulation of recombinant hNav1.5 α subunits. Biochemical and Biophysical Research Communications, 324(4), 1289-1295.
- H2O2 regulates recombinant Ca2+ channel α1C subunits but does not mediate their sensitivity to acute hypoxia. Biochemical and Biophysical Research Communications, 318(1), 135-141.
- Novel level of signalling control in the JAK/STAT pathway revealed by in situ visualisation of protein-protein interaction during Drosophiladevelopment. Development, 130(14), 3077-3084.
- The Fertile Field of Drosophila JAK/STAT Signalling. Current Biology, 12(16), R569-R575.
- Identification of the first invertebrate interleukin JAK/STAT receptor, the Drosophila gene domeless. Current Biology, 11(21), 1700-1705.
- Drosophila grain encodes a GATA transcription factor required for cell rearrangement during morphogenesis. Development, 127(22), 4867-4876.
- A transgene with repeated DNA causes high frequency, post-transcriptional suppression of ACC-oxidase gene expression in tomato. The Plant Journal, 15(6), 737-746.
- Ethylene receptor expression is regulated during fruit ripening, flower senescence and abscission. Plant Molecular Biology, 31(6), 1227-1231.
- Designing RNAi Screens to Identify JAK/STAT Pathway Components, Methods in Molecular Biology (pp. 81-97). Humana Press
Conference proceedings papers
- Generation of a model of iPSC-derived human intestinal organoids. HUMAN GENE THERAPY, Vol. 28(8) (pp A26-A26)
- Methotrexate Is a Suppressor of JAK/STAT Pathway Activation Which Inhibits JAK2V617F Induced Signalling. Blood, Vol. 124(21) (pp 4577-4577)
- Regulation of Oxygen Sensitivity in Adrenal Chromaffin Cells. Annals of the New York Academy of Sciences, Vol. 1177(1) (pp 132-139)
- 18-P019 The University of Sheffield RNAi Screening facility: Genome-wide RNAi screens in Drosophila cells using a 2nd generation in silico optimized library. Mechanisms of Development, Vol. 126 (pp S290-S290)
- Acute Hypoxic Regulation of Recombinant THIK-1 Stably Expressed in HEK293 Cells (pp 203-208)
- Chemically modified dsRNA induces RNAi effects in insects in vitro and in vivo: A potential new tool for improving RNA-based plant protection, Cold Spring Harbor Laboratory.
- Comprehensive functional profiling of long non-coding RNAs through a novel pan-cancer integration approach and modular analysis of their protein-coding gene association networks, Cold Spring Harbor Laboratory.
- A genome-wide RNAi screen identifies MASK as a positive regulator of cytokine receptor stability, Cold Spring Harbor Laboratory.
- Fabrication of antibody functionalized silk fibroin micro-well arrays using reactive inkjet printing for circulating tumour cell capture. Dr. X. Zhao (Chem Eng) and Dr. S. Brown
- Engineering and Physical Sciences Research Council. Nov 2016 - Oct 2019 (£333,657)
- Teaching activities
Undergraduate and postgraduate taught modules
- BMS109 1st year Tutor
- BMS110 Research Topics in Biomedicine
- BMS356 Biomedical Technology & Drug Discovery
- Level 3 Practical and Dissertation Modules
- BMS6011 The Biotech and Pharmaceutical Industry
- BMS6012 3D Tissue Culture and Genome Editing
- BMS6013 Small Molecule and Functional Genomic Screening
- BMS6014 Genomic Approaches to Drug Discovery
- MSc Genomic Approaches to Drug Discovery (module leader)
- Professional activities and memberships
- Founding member and co-organiser of the UK RNAi and Functional Genomics Group (London, 2011-)
- Founding member and co-organiser of the Stem Cell Phenotyping Consortium (2014-)
- EMBO Practical Course Tutor; High-throughput RNAi and Data Analysis (2010)
- SRSF Symposium co-organiser (Sheffield, 2010)
- Guest Editor of Seminars in Cell and Developmental Biology (2008)
- MRC Career Development Fellowship Award 2004-2008
Postgraduate studentship opportunities
We advertise PhD opportunities (Funded or Self-Funded) on FindAPhD.com
For further information and details of other projects on offer, please see the department PhD Opportunities page.