Dr Phil Jackson
School of Biosciences
Research Fellow
+44 114 222 2813
Firth Court
Full contact details
Dr Phil Jackson
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
- Profile
-
- 2021-present: Research Fellow, School of Biosciences, University of Sheffield.
- 2019-2021: Research Fellow, Dept. of Chemical and Biological Engineering, University of Sheffield.
- 2010-2018: Postdoctoral Research Associate, Dept. of Chemical and Biological Engineering, University of Sheffield.
- 1989-2010: Senior Product Application Specialist, Applied Biosystems.
- 1988-1989: Postdoctoral Research Associate, Dept. of Biochemistry, University of Leeds.
- 1987-1988: Postdoctoral Research Associate, Medical School, University of Leeds.
- 1983-1987: PhD ‘The control of ATP synthesis in heart mitochondria’, Dept. of Biochemistry, University of Leeds.
- Research interests
-
My background is in the characterisation and quantification of proteins and peptides by mass spectrometry, with a diverse range of projects including the proteomic analysis of pathogenic bacteria and the development of quantitative assays for human tumour markers.
Since joining the University in 2010, I have worked in the research groups of Professors Neil Hunter FRS and Mark Dickman (Chemical and Biological Engineering) and more recently with Prof. Matt Johnson and Dr Andy Hitchcock. Outside Sheffield, I work on collaborative projects with Prof. Josef Komenda, Dr Roman Sobotka, and Dr Vendula Krynická (Institute of Microbiology, The Czech Academy of Sciences, Třeboň, Czech Republic).
My input into our photosynthesis-based research programmes, involving purple bacterial, cyanobacterial, and eukaryotic model organisms, is focused on the following themes:- Proteomic analysis of complexes involved in solar energy capture and electron transport to support structure determination by cryo-EM.
- Quantitative proteomics of photosynthetic membranes in conjunction with targeted mutations to investigate adaptive mechanisms.
- Analysis of protein-protein interactions within photosynthetic energy transducing complexes by affinity tagging and covalent crosslinking.
- Publications
-
Show: Featured publications All publications
Featured publications
Journal articles
- Cryo-EM structures of light-harvesting 2 complexes from
Rhodopseudomonas palustris
reveal the molecular origin of absorption tuning. Proceedings of the National Academy of Sciences, 119(43).
- 2.4-Å structure of the double-ring Gemmatimonas phototrophica photosystem. Science Advances, 8(7). View this article in WRRO
- Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation. Science Advances, 8(6). View this article in WRRO
- Cryo-EM structure of the dimeric Rhodobacter sphaeroides RC-LH1 core complex at 2.9 Å : the structural basis for dimerisation. Biochemical Journal, 478(21), 3923-3937. View this article in WRRO
- Cryo-EM structure of the monomeric Rhodobacter sphaeroides RC–LH1 core complex at 2.5 Å. Biochemical Journal, 478(20), 3775-3790. View this article in WRRO
- Comparative proteomics of thylakoids from Arabidopsis grown in laboratory and field conditions. Plant Direct, 5(10). View this article in WRRO
- Genes linking copper trafficking and homeostasis to the biogenesis and activity of the cbb3-type cytochrome c oxidase in the enteric pathogen Campylobacter jejuni. Frontiers in Microbiology, 12. View this article in WRRO
- How the O2-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls. Nature Plants, 7(3), 365-375. View this article in WRRO
- Structures of Rhodopseudomonas palustris RC-LH1 complexes with open or closed quinone channels. Science Advances, 7(3). View this article in WRRO
- Developmental acclimation of the thylakoid proteome to light intensity in Arabidopsis. The Plant Journal, 105(1), 223-244. View this article in WRRO
- Xanthophyll carotenoids stabilise the association of cyanobacterial chlorophyll synthase with the LHC-like protein HliD. Biochemical Journal, 477(20), 4021-4036. View this article in WRRO
- Proteorhodopsin overproduction enhances the long-term viability of Escherichia coli. Applied and Environmental Microbiology, 86(1). View this article in WRRO
- Depletion of the FtsH1/3 Proteolytic Complex Suppresses the Nutrient Stress Response in the Cyanobacterium Synechocystis sp strain PCC 6803. The Plant Cell, 31(12), 2912-2928.
- Membrane organization of photosystem I complexes in the most abundant phototroph on Earth. Nature Plants, 5(8), 879-889. View this article in WRRO
- The ChlD subunit links the motor and porphyrin binding subunits of magnesium chelatase. Biochemical Journal, 476(13), 1875-1887. View this article in WRRO
- Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase. FEBS Letters, 592(18), 3062-3073. View this article in WRRO
- Probing the local lipid environment of the Rhodobacter sphaeroides cytochrome bc1 and Synechocystis sp. PCC 6803 cytochrome b6f complexes with styrene maleic acid. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1859(3), 215-225. View this article in WRRO
- Identification of protein W, the elusive sixth subunit of the Rhodopseudomonas palustris reaction center-light harvesting 1 core complex. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1859(2), 119-128. View this article in WRRO
- PufQ regulates porphyrin flux at the haem/bacteriochlorophyll branchpoint of tetrapyrrole biosynthesis via interactions with ferrochelatase.. Molecular Microbiology. View this article in WRRO
- Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll–Protein Complex. ACS Synthetic Biology, 5(9), 948-954. View this article in WRRO
- Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina. Journal of Bacteriology, 198(9), 1393-1400. View this article in WRRO
- Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803. Frontiers in Plant Science, 7. View this article in WRRO
- Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1847(2), 189-201. View this article in WRRO
- Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force Microscopy. Journal of Biological Chemistry, 289(43), 29927-29936. View this article in WRRO
- Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides.. Biochim Biophys Acta, 1837(10), 1769-1780. View this article in WRRO
- Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1837, e118-e118.
- A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase.. Plant Cell, 26(3), 1267-1279.
- Three-dimensional structure of the Rhodobacter sphaeroides RC-LH1-PufX complex: dimerization and quinone channels promoted by PufX.. Biochemistry, 52(43), 7575-7585.
- Identification of an 8-vinyl reductase involved in bacteriochlorophyll biosynthesis in Rhodobacter sphaeroides and evidence for the existence of a third distinct class of the enzyme.. Biochem J, 450(2), 397-405.
- Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803.. J Biol Chem, 287(33), 27823-27833.
- Quantitative proteomic analysis of intracytoplasmic membrane development in Rhodobacter sphaeroides.. Mol Microbiol, 84(6), 1062-1078.
- Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities.. ISME J, 6(4), 875-885.
All publications
Journal articles
- Cryo-EM structure of the four-subunit
Rhodobacter sphaeroides
cytochrome
bc
1
complex in styrene maleic acid nanodiscs. Proceedings of the National Academy of Sciences, 120(12).
- Absolute quantification of cellular levels of photosynthesis-related proteins in Synechocystis sp. PCC 6803. Photosynthesis Research.
- FtsH4 protease controls the biogenesis of PSII complex by dual regulation of high light-inducible proteins. Plant Communications.
- Cryo-EM structures of light-harvesting 2 complexes from
Rhodopseudomonas palustris
reveal the molecular origin of absorption tuning. Proceedings of the National Academy of Sciences, 119(43).
- 2.4-Å structure of the double-ring Gemmatimonas phototrophica photosystem. Science Advances, 8(7). View this article in WRRO
- Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation. Science Advances, 8(6). View this article in WRRO
- Cryo-EM structure of the dimeric Rhodobacter sphaeroides RC-LH1 core complex at 2.9 Å : the structural basis for dimerisation. Biochemical Journal, 478(21), 3923-3937. View this article in WRRO
- Cryo-EM structure of the monomeric Rhodobacter sphaeroides RC–LH1 core complex at 2.5 Å. Biochemical Journal, 478(20), 3775-3790. View this article in WRRO
- Comparative proteomics of thylakoids from Arabidopsis grown in laboratory and field conditions. Plant Direct, 5(10). View this article in WRRO
- Genes linking copper trafficking and homeostasis to the biogenesis and activity of the cbb3-type cytochrome c oxidase in the enteric pathogen Campylobacter jejuni. Frontiers in Microbiology, 12. View this article in WRRO
- How the O2-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls. Nature Plants, 7(3), 365-375. View this article in WRRO
- Structures of Rhodopseudomonas palustris RC-LH1 complexes with open or closed quinone channels. Science Advances, 7(3). View this article in WRRO
- Developmental acclimation of the thylakoid proteome to light intensity in Arabidopsis. The Plant Journal, 105(1), 223-244. View this article in WRRO
- Xanthophyll carotenoids stabilise the association of cyanobacterial chlorophyll synthase with the LHC-like protein HliD. Biochemical Journal, 477(20), 4021-4036. View this article in WRRO
- Proteorhodopsin overproduction enhances the long-term viability of Escherichia coli. Applied and Environmental Microbiology, 86(1). View this article in WRRO
- Depletion of the FtsH1/3 Proteolytic Complex Suppresses the Nutrient Stress Response in the Cyanobacterium Synechocystis sp strain PCC 6803. The Plant Cell, 31(12), 2912-2928.
- Membrane organization of photosystem I complexes in the most abundant phototroph on Earth. Nature Plants, 5(8), 879-889. View this article in WRRO
- The ChlD subunit links the motor and porphyrin binding subunits of magnesium chelatase. Biochemical Journal, 476(13), 1875-1887. View this article in WRRO
- Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase. FEBS Letters, 592(18), 3062-3073. View this article in WRRO
- Probing the local lipid environment of the Rhodobacter sphaeroides cytochrome bc1 and Synechocystis sp. PCC 6803 cytochrome b6f complexes with styrene maleic acid. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1859(3), 215-225. View this article in WRRO
- Identification of protein W, the elusive sixth subunit of the Rhodopseudomonas palustris reaction center-light harvesting 1 core complex. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1859(2), 119-128. View this article in WRRO
- PufQ regulates porphyrin flux at the haem/bacteriochlorophyll branchpoint of tetrapyrrole biosynthesis via interactions with ferrochelatase.. Molecular Microbiology. View this article in WRRO
- Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll–Protein Complex. ACS Synthetic Biology, 5(9), 948-954. View this article in WRRO
- Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina. Journal of Bacteriology, 198(9), 1393-1400. View this article in WRRO
- Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803. Frontiers in Plant Science, 7. View this article in WRRO
- Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1847(2), 189-201. View this article in WRRO
- Aberrant Assembly Complexes of the Reaction Center Light-harvesting 1 PufX (RC-LH1-PufX) Core Complex of Rhodobacter sphaeroides Imaged by Atomic Force Microscopy. Journal of Biological Chemistry, 289(43), 29927-29936. View this article in WRRO
- Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides.. Biochim Biophys Acta, 1837(10), 1769-1780. View this article in WRRO
- Integration of energy and electron transfer processes in the photosynthetic membrane of Rhodobacter sphaeroides. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1837, e118-e118.
- A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase.. Plant Cell, 26(3), 1267-1279.
- Three-dimensional structure of the Rhodobacter sphaeroides RC-LH1-PufX complex: dimerization and quinone channels promoted by PufX.. Biochemistry, 52(43), 7575-7585.
- Identification of an 8-vinyl reductase involved in bacteriochlorophyll biosynthesis in Rhodobacter sphaeroides and evidence for the existence of a third distinct class of the enzyme.. Biochem J, 450(2), 397-405.
- Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803.. J Biol Chem, 287(33), 27823-27833.
- Quantitative proteomic analysis of intracytoplasmic membrane development in Rhodobacter sphaeroides.. Mol Microbiol, 84(6), 1062-1078.
- 2-Carboxy-D-arabinitol 1-phosphate (CA1P) phosphatase: Evidence for a wider role in plant Rubisco regulation. Biochemical Journal, 442(3), 733-742.
- Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities.. ISME J, 6(4), 875-885.
- Multidimensional proteomic analysis of the soluble subproteome of the emerging nosocomial pathogen Ochrobactrum anthropi. J PROTEOME RES, 5(11), 3145-3153.
- A combined shotgun and multidimensional proteomic analysis of the insoluble subproteome of the obligate thermophile, Geobacillus thermoleovorans T80. J PROTEOME RES, 5(9), 2465-2473.
- Origin of dimeric structure in the ribonuclease superfamily. BIOCHEMISTRY-US, 37(12), 4008-4022.
- Protease digestion studies of an equilibrium intermediate in the unfolding of creatine kinase. BIOCHEM J, 321, 83-88.
- THE OCCURRENCE OF THE THAUMATIN-LIKE, PATHOGENESIS-RELATED PROTEIN, PR-5, IN INTERCELLULAR FLUIDS FROM NICOTIANA SPECIES AND FROM AN INTERSPECIFIC NICOTIANA HYBRID. PHYSIOL MOL PLANT P, 41(1), 1-10.
- STRUCTURE OF A 16 KDA INTEGRAL MEMBRANE-PROTEIN THAT HAS IDENTITY TO THE PUTATIVE PROTON CHANNEL OF THE VACUOLAR H+ -ATPASE. PROTEIN ENG, 5(1), 7-15.
- IDENTIFICATION BY PROTEIN MICROSEQUENCING OF A PROTEINASE-V8-CLEAVAGE SITE IN A FOLDING INTERMEDIATE OF CHICK MUSCLE CREATINE-KINASE. BIOCHEM J, 280, 809-811.
- PURIFICATION AND CHARACTERIZATION OF HMW GLUTENIN SUBUNITS ENCODED BY CHROMOSOME-1B OF DURUM-WHEAT (TRITICUM-DURUM). J CEREAL SCI, 14(2), 111-116.
- A class of amphipathic proteins associated with lipid storage bodies in plants. Possible similarities with animal serum apolipoproteins. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1088(1), 86-94.
- CHARACTERIZATION OF THE MAJOR PROTEIN-COMPONENT FROM ALEURONE CELLS OF BARLEY (HORDEUM-VULGARE L). J EXP BOT, 41(225), 385-392.
- THE PRESENCE OF A THAUMATIN-LIKE PROTEIN, A CHITINASE AND A GLUCANASE AMONG THE PATHOGENESIS-RELATED PROTEINS OF POTATO (SOLANUM-TUBEROSUM). PHYSIOL MOL PLANT P, 36(4), 325-338.
- Purification and partial amino acid sequence of human urine protein 1. Evidence for homology with rabbit uteroglobin.. J Chromatogr, 452, 359-367.
- The mitochondrial ATP synthase inhibitor protein binds near the C-terminus of the F1 beta-subunit.. FEBS Lett, 229(1), 224-228.
- INTERACTION BETWEEN THE SOLUBLE-F1 ATPASE AND ITS NATURALLY-OCCURRING INHIBITOR PROTEIN - STUDIES USING HYDROPHILIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND IMMUNOELECTRON MICROSCOPY. EUR J BIOCHEM, 157(1), 181-186.
- SITES OF PROTEIN PROTEIN-INTERACTION ON THE MITOCHONDRIAL F1-ATPASE INHIBITOR PROTEIN. BIOCHEM J, 235(2), 577-583.
- INTERACTION BETWEEN F1-ATPASE AND ITS NATURALLY-OCCURRING INHIBITOR PROTEIN - STUDIES USING A SPECIFIC ANTI-INHIBITOR ANTIBODY. BIOCHIM BIOPHYS ACTA, 806(1), 64-74.
- BINDING OF MITOCHONDRIAL ATPASE FROM OX HEART TO ITS NATURALLY-OCCURRING INHIBITOR PROTEIN - LOCALIZATION BY ANTIBODY-BINDING. BIOSCIENCE REP, 3(10), 921-926.
- SYNTHESIS OF ANDROGEN-DEPENDENT SECRETORY PROTEINS BY RAT SEMINAL-VESICLES. MOL CELL ENDOCRINOL, 21(3), 255-262.
- FUNCTIONAL-DEVELOPMENT OF SEX ACCESSORY-ORGANS OF THE MALE-RAT - USE OF ESTRADIOL BENZOATE TO IDENTIFY THE NEONATAL-PERIOD AS CRITICAL FOR DEVELOPMENT OF NORMAL PROTEIN-SYNTHETIC AND SECRETORY CAPABILITIES. BIOCHEM J, 194(3), 895-905.
- ANDROGENIC REGULATION OF MESSENGER-RNA SEQUENCE COMPLEXITY IN ACCESSORY SEXUAL TISSUES OF THE MALE-RAT STUDIED WITH FRACTIONATED COMPLEMENTARY-DNA. EUR J BIOCHEM, 102(2), 431-440.
-
STN7 is not essential for developmental acclimation of Arabidopsis to light intensity. The Plant Journal.
- High cyclic electron transfer via the PGR5 pathway in the absence of photosynthetic control. Plant Physiology.
- Production of Trimeric SARS‐CoV‐2 Spike Protein by CHO Cells for Serological COVID‐19 Testing. Biotechnology and Bioengineering.
Chapters
- N-terminal protein sequencing for special applications. (pp. 287-300).
- Validation of protein sequencing in a regulated laboratory. (pp. 309-318).
Conference proceedings papers
- PROBING PROTEIN-STRUCTURE WITH PROTEASES - STUDIES OF AN EQUILIBRIUM INTERMEDIATE IN PROTEIN UNFOLDING. BIOCHEMICAL SOCIETY TRANSACTIONS, Vol. 23(3) (pp S477-S477)
- EVIDENCE FOR A HUMAN UTEROGLOBIN-LIKE PROTEIN OCCURRING IN THE URINE OF PATIENTS WITH RENAL-FAILURE. BIOCHEM SOC T, Vol. 16(6) (pp 970-971)
- Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing.. Ann Clin Biochem, Vol. 25 ( Pt 3) (pp 319-324). England
- GEL-PERMEATION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN THE STUDY OF BINDING BETWEEN SOLUBLE F1-ATPASE AND ITS NATURALLY-OCCURRING INHIBITOR PROTEIN. BIOCHEM SOC T, Vol. 14(6) (pp 1199-1200)
- SITES OF PROTEIN PROTEIN-INTERACTION ON THE MITOCHONDRIAL ATPASE INHIBITOR PROTEIN. BIOCHEM SOC T, Vol. 14(1) (pp 143-144)
- PROTEIN INHIBITORS OF THE MITOCHONDRIAL ATPASE FROM MAMMALIAN-TISSUES. BIOCHEM SOC T, Vol. 13(4) (pp 748-749)
- IMMUNOLOGICAL CHARACTERIZATION OF THE INTERACTION BETWEEN THE F1-ATPASE FROM OX HEART-MITOCHONDRIA AND ITS NATURALLY-OCCURRING INHIBITOR PROTEIN. BIOCHEM SOC T, Vol. 13(1) (pp 226-226)
Preprints
- Supercharged PGR5-dependent cyclic electron transfer compensates for mis-regulated chloroplast ATP synthase, Cold Spring Harbor Laboratory.
- Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Cold Spring Harbor Laboratory.
- Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 Testing, Authorea, Inc..
- Purification of recombinant SARS-CoV-2 spike, its receptor binding domain, and CR3022 mAb for serological assay.
- Cryo-EM structures of light-harvesting 2 complexes from
Rhodopseudomonas palustris
reveal the molecular origin of absorption tuning. Proceedings of the National Academy of Sciences, 119(43).
- Teaching activities
-
Level 3 modules
MBB380 Research project
- Professional activities and memberships
-
- Invited speaker: ERC PhotoRedesign Collaborators’ Conference, Munich, Germany (2022).
- Invited speaker: Scientific Advances in Metabolomics and Systems Biology Symposium, Zurich, Switzerland (2018).
- Invited seminar: Institute of Microbiology, The Czech Academy of Sciences, Třeboň, Czech Republic (2017).
- Visiting researcher: Washington University in St Louis, USA (2015).
- Invited seminar: Midwest Mass Spectometry Discussion Group, St Louis, USA (2014).
- Invited speaker: ProteoMMX Conference, Chester, UK (2014).
- Visiting researcher: Washington University in St Louis, USA (2011).