Dr Anne-Gaelle Borycki
Room: D19 Firth Court building
Brief career history
The Borycki lab uses primarily mouse genetics to study how the extra-cellular matrix, Sonic hedgehog signaling and cellular structures such as the primary cilum contribute to the regulation of satellite cell activity and to skeletal muscle regeneration.
Satellite cell biology and skeletal muscle regeneration
Satellite cells are skeletal muscle-specific stem cells that reside in a sub-laminal position at the periphery of myofibres. Satellite cells are normally quiescent, but become activated and expand rapidly to produce new muscle progenitor cells that differentiate and fuse to damaged fibres to repair diseased or injured muscles. Therefore, satellite cells hold the promise to be harnessed for the treatment of muscular dystrophies or age-related sarcopenia. However, the use of satellite cells for therapeutic purpose requires prior knowledge of their biology.
Research in our lab focuses on several aspects of satellite cell biology:
To investigate these issues, we use a combination of ex-vivo and in-vivo approaches in the mouse, as well as mouse genetic tools such as conditional knockout, transgenic reporter, and gain-of-function mutant lines.
Our current external collaborations include:
Peter Zammit (UCL), Jennifer Morgan (UCL), Paolo de Coppi (UCL), James Briscoe (Crick Institute), Olivier Lefebvre (Strasbourg).
Undergraduate and postgraduate taught modules
Level 4 Tutor
- Pickering J, Cunliffe VT, Van Eeden F & Borycki A-G (2016) Hedgehog signalling acts upstream of Laminin alpha1 transcription in the zebrafish paraxial mesoderm. Matrix Biology. View this article in WRRO
- Jaafar Marican H, Cruz Migoni S & Borycki AG (2016) Asymmetric Distribution of Primary Cilia Allocates Satellite Cells for Self-Renewal. Stem Cell Reports, 6(6), 788-805. View this article in WRRO
- Borycki AG (2013) The myotomal basement membrane: Insight into laminin-111 function and its control by Sonic hedgehog signaling. Cell Adhesion and Migration, 7(1), 72-81.
- Anderson C, Williams VC, Moyon B, Daubas P, Tajbakhsh S, Buckingham ME, Shiroishi T, Hughes SM & Borycki AG (2012) Sonic hedgehog acts cell-autonomously on muscle precursor cells to generate limb muscle diversity. Genes and Development, 26(18), 2103-2117.
- Anderson C, Thorsteinsdóttir S & Borycki A-G (2009) Sonic hedgehog-dependent synthesis of laminin α1 controls basement membrane assembly in the myotome. Development, 136(20), 3495-3504.
- Rayagiri SS, Ranaldi D, Raven A, Mohamad Azhar IF, Lefebvre O, Zammit PS & Borycki AG () Basal lamina remodeling at the skeletal muscle stem cell niche mediates stem cell self-renewal. Nature Communications.