Dr Jason King
Advanced Vice-Chancellor’s Fellow
Room: D09 Florey building
The main focus of the laboratory is to understand how cells perform macropinocytosis – the bulk capture of extracellular fluid. This plays important and distinct roles in diverse cell types such as macrophages, dendritic cells and neurons, by allowing cells to sample their environment and regulating membrane turnover. However macropinocytosis also allows cancer cells to scavenge the extracellular nutrients required to support their growth, and provides a route for pathogens and prions to enter host cells.
The diverse importance of macropinocytosis has only recently become clear, and both the formation and maturation of macropinosomes is poorly understood. My laboratory is thus trying to answer two fundamental questions:
Please see our personal lab website for more information on our work
Autophagy and lysosomal degradation pathways
In collaboration with Cecile Perrault (Mechanical engineering), and Paul Evans (Medical School) we are now extending these studies to physiologically relevant cells and tissues. This will further our understanding of this pathway, and determine the role of mechanically-induced autophagy in cellular homeostasis.
Our primary experimental system is the soil amoeba Dictyostelium discoideum. This allows us to use powerful molecular techniques to dissect autophagy in a simple model system. In addition, as Dictyostelium exclusively use phagocytosis and macropinocytosis to take up nutrients, they are an excellent model for phagocytic immune cells. The ease with which Dictyostelium can be genetically manipulated allows us to circumvent the experimental limitations of macrophages and neutrophils, which we are currently exploiting to understand both infectious disease and fluid uptake, in collaboration with Simon Johnston.
Undergraduate and postgraduate taught modules
Postgraduate studentship opportunities
We advertise PhD opportunities (Funded or Self-Funded) on FindAPhD.com
For further information and details of other projects on offer, please see the department PhD Opportunities page:
- Buckley CM, Heath VL, Guého A, Bosmani C, Knobloch P, Sikakana P, Personnic N, Dove SK, Michell RH, Meier R , Hilbi H et al (2019) PIKfyve/Fab1 is required for efficient V-ATPase and hydrolase delivery to phagosomes, phagosomal killing, and restriction of Legionella infection. PLoS Pathogens, 15(2). View this article in WRRO
- Buckley CM, Gopaldass N, Bosmani C, Johnston SA, Soldati T, Insall RH & King JS (2016) WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors. Proc Natl Acad Sci U S A, 113(40), E5906-E5915. View this article in WRRO
- Gerstenmaier L, Pilla R, Herrmann L, Herrmann H, Prado M, Villafano GJ, Kolonko M, Reimer R, Soldati T, King JS & Hagedorn M (2015) The autophagic machinery ensures nonlytic transmission of mycobacteria. Proceedings of the National Academy of Sciences, 112(7), E687-E692.
- Calvo-Garrido J, King JS, Muñoz-Braceras S & Escalante R (2014) Vmp1 Regulates PtdIns3P Signaling During Autophagosome Formation inDictyostelium discoideum. Traffic, 15(11), 1235-1246.
- King JS, Gueho A, Hagedorn M, Gopaldass N, Leuba F, Soldati T & Insall RH (2013) WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion. Molecular Biology of the Cell, 24(17), 2714-2726. View this article in WRRO
- King JS, Veltman DM & Insall RH (2011) The induction of autophagy by mechanical stress. AUTOPHAGY, 7(12), 1490-1499.